##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936623.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434681
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.141759129108472	31.0	31.0	33.0	28.0	34.0
2	31.212820436135924	31.0	31.0	34.0	28.0	34.0
3	30.944255672550675	31.0	31.0	34.0	27.0	34.0
4	34.90868475962832	35.0	35.0	37.0	32.0	37.0
5	35.163936311916096	35.0	35.0	37.0	33.0	37.0
6	35.141789036097734	36.0	35.0	37.0	32.0	37.0
7	30.826037945067764	35.0	35.0	37.0	0.0	37.0
8	32.98229736289371	35.0	35.0	37.0	17.0	37.0
9	36.28728193778886	39.0	35.0	39.0	32.0	39.0
10	36.200224992580765	37.0	35.0	39.0	32.0	39.0
11	36.36600863621828	38.0	35.0	39.0	32.0	39.0
12	36.29165525983422	38.0	35.0	39.0	32.0	39.0
13	36.06779684412247	38.0	35.0	39.0	31.0	39.0
14	36.85630611873995	39.0	35.0	40.0	31.0	41.0
15	37.14449676889489	39.0	35.0	41.0	31.0	41.0
16	37.20070120387135	39.0	35.0	40.0	32.0	41.0
17	37.14395844308815	39.0	35.0	40.0	32.0	41.0
18	37.06602312960539	39.0	35.0	40.0	32.0	41.0
19	36.83849075528951	38.0	35.0	40.0	31.0	41.0
20	36.77051907030673	38.0	35.0	40.0	31.0	41.0
21	36.76503918965862	38.0	35.0	40.0	31.0	41.0
22	36.91486860479294	39.0	35.0	40.0	32.0	41.0
23	36.9949204129005	39.0	35.0	40.0	32.0	41.0
24	36.92183233221604	39.0	35.0	40.0	32.0	41.0
25	36.62019503958075	38.0	35.0	40.0	31.0	41.0
26	36.576638960525074	38.0	35.0	40.0	31.0	41.0
27	36.60641711968087	38.0	35.0	40.0	31.0	41.0
28	36.59514218472857	38.0	35.0	40.0	31.0	41.0
29	36.54626266158401	38.0	35.0	40.0	31.0	41.0
30	36.318350698558255	38.0	35.0	40.0	30.0	41.0
31	36.20285220656067	38.0	35.0	40.0	30.0	41.0
32	36.00949661935994	38.0	35.0	40.0	30.0	41.0
33	35.84000220851613	38.0	35.0	40.0	29.0	41.0
34	35.66705469068121	38.0	35.0	40.0	29.0	41.0
35	35.549695063736394	38.0	34.0	40.0	28.0	41.0
36	35.44801590131614	38.0	34.0	40.0	27.0	41.0
37	35.31435926576041	38.0	34.0	40.0	27.0	41.0
38	35.15068521513478	38.0	34.0	40.0	26.0	41.0
39	35.074969920470416	38.0	34.0	40.0	25.0	41.0
40	34.93703198437475	38.0	34.0	40.0	24.0	41.0
41	34.755629990728835	38.0	34.0	40.0	24.0	41.0
42	34.764314520303394	38.0	34.0	40.0	24.0	41.0
43	34.74921839233829	37.0	34.0	40.0	24.0	41.0
44	34.547088554595206	37.0	34.0	40.0	23.0	41.0
45	34.479004143268284	37.0	33.0	40.0	23.0	41.0
46	34.45329333465231	37.0	33.0	40.0	23.0	41.0
47	34.36033090933351	37.0	33.0	40.0	23.0	41.0
48	34.26244533347443	37.0	33.0	40.0	23.0	41.0
49	34.1804587732153	36.0	33.0	40.0	23.0	41.0
50	34.019540766677174	36.0	33.0	40.0	23.0	41.0
51	32.340877563086494	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	20.0
9	40.0
10	31.0
11	45.0
12	23.0
13	28.0
14	26.0
15	24.0
16	75.0
17	143.0
18	276.0
19	498.0
20	820.0
21	1234.0
22	1856.0
23	2677.0
24	3928.0
25	5372.0
26	6899.0
27	7602.0
28	8262.0
29	9361.0
30	11119.0
31	13927.0
32	18215.0
33	25440.0
34	38794.0
35	38899.0
36	42888.0
37	61807.0
38	76834.0
39	57515.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08028416240875	19.865372537562028	24.369135066865127	13.68520823316409
2	35.8041874386044	23.102228990915176	24.32588495931499	16.76769861116543
3	32.28781566252033	22.96235630266793	28.773744424071907	15.97608361073983
4	28.891762004780517	24.46023635723669	28.540930015344586	18.10707162263821
5	25.832507056899196	28.468002972294627	27.19419528343774	18.505294687368437
6	26.169535820521254	32.81739942624591	27.30301071360377	13.71005403962906
7	66.4687437454133	18.166425493637863	11.68328038262542	3.6815503783234145
8	77.76622396654098	6.439664949698745	11.403074898603803	4.391036185156471
9	71.73421428587861	6.856062261750571	13.96403339460432	7.445690057766501
10	45.01899093818225	22.454167538953854	19.627266892272726	12.899574630591168
11	34.703610233711615	23.344015496421513	25.402766626560624	16.549607643306242
12	31.096827328546684	20.95881807578431	29.48000947821506	18.46434511745395
13	26.74743087459539	23.637794152493438	32.224320823776516	17.39045414913465
14	20.767183290735044	26.582482326119617	32.755745017610614	19.894589365534728
15	18.917781085439668	23.648146571853843	38.421048999151104	19.01302334355539
16	24.710074744467782	23.477676733052512	32.537423995987865	19.27482452649184
17	25.255992325406446	23.175386087728704	29.28676431682084	22.281857270044007
18	26.829790121951504	22.393433345372813	31.280410231871187	19.4963663008045
19	26.07360340111484	24.289076357144666	28.234728456040177	21.402591785700317
20	25.51434270188943	26.264548024873413	29.852696575189626	18.368412698047536
21	23.774216034287214	26.075673884986923	31.60915706000492	18.540953020720945
22	23.70589006650854	21.761705710624575	32.39663109268636	22.13577313018052
23	22.00188183978596	25.188586572682038	31.48492802768007	21.324603559851937
24	23.47077512014558	24.025894851626823	30.764859747723044	21.738470280504554
25	23.073242216706046	26.00159657311914	29.277332112514692	21.647829097660125
26	21.630345011629217	25.306604153390648	29.54603490835808	23.51701592662205
27	21.72420694716355	25.694704852524037	31.13869711351543	21.442391086796984
28	19.91644447307336	25.561043615893038	31.79849130741854	22.724020603615063
29	22.02925823765014	25.749457648252395	30.7729116294478	21.448372484649664
30	24.516369475546433	24.480711142193933	30.6282078121657	20.374711570093933
31	26.062560820463744	24.37235581955503	29.550866037392936	20.01421732258829
32	26.70671135844447	25.289580174886872	27.914723670921894	20.088984795746768
33	26.312169153931276	24.607470765917995	27.14013264900007	21.94022743115066
34	22.608993721832793	24.27918404531139	31.876019425739795	21.235802807116023
35	22.93935092631148	25.89646200317014	29.604928671830606	21.55925839868777
36	27.23836560604213	25.05492533605104	28.336872327062835	19.369836730843996
37	24.299888884032196	26.961610928473984	28.466392595949674	20.272107591544145
38	24.968885228477895	26.808855229467127	26.43547797120187	21.78678157085311
39	23.410731087855233	23.83472017410469	28.752119370296835	24.002429367743243
40	25.685042594454323	23.41326167925444	29.254096682394675	21.64759904389656
41	23.247392915724404	23.077383184450206	28.554963294922025	25.120260604903365
42	24.35418157223343	23.218636195278837	29.62931437076845	22.797867861719283
43	25.002012970431192	23.15100038879086	28.931791359640748	22.9151952811372
44	23.471235227672707	24.00795065806879	28.792838886447765	23.727975227810738
45	21.710863828876807	24.48370184112027	27.782442756872282	26.02299157313064
46	23.736487217062628	25.298322217902324	27.74816474610116	23.217025818933884
47	21.605039097637118	24.73123969071572	31.48124716746303	22.182474044184126
48	23.69553764714814	25.583358830958797	28.624209477754953	22.096894044138114
49	23.794690819244458	23.42798512012257	30.459118295945764	22.318205764687207
50	21.60043802236583	24.793584260641712	29.50738587607924	24.098591840913222
51	21.632875603028427	24.806467271401324	28.040102972064574	25.520554153505675
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	132.0
1	541.0
2	950.0
3	3784.0
4	6618.0
5	4403.5
6	2189.0
7	2055.5
8	1922.0
9	1897.0
10	1872.0
11	1765.5
12	1659.0
13	1762.0
14	1865.0
15	1718.5
16	1572.0
17	1448.0
18	1324.0
19	1425.0
20	1526.0
21	1642.5
22	1759.0
23	1769.0
24	1779.0
25	2327.5
26	3416.5
27	3957.0
28	4391.0
29	4825.0
30	5842.5
31	6860.0
32	7669.5
33	8479.0
34	9472.0
35	10465.0
36	11172.0
37	11879.0
38	13055.0
39	14231.0
40	16211.0
41	18191.0
42	20088.5
43	21986.0
44	23327.0
45	24668.0
46	26883.5
47	29099.0
48	33699.0
49	38299.0
50	37049.0
51	35799.0
52	35123.0
53	34447.0
54	31760.5
55	29074.0
56	26982.0
57	24890.0
58	23886.5
59	22883.0
60	20615.5
61	18348.0
62	16445.5
63	14543.0
64	12728.0
65	10913.0
66	9415.0
67	7917.0
68	6814.0
69	5711.0
70	4922.5
71	4134.0
72	3256.5
73	2379.0
74	1806.0
75	958.5
76	684.0
77	531.5
78	379.0
79	287.5
80	196.0
81	121.5
82	47.0
83	33.5
84	20.0
85	43.0
86	66.0
87	41.0
88	16.0
89	10.5
90	5.0
91	7.0
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434681.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.4192835568809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.30138333421618	25.165182818715557
2	8.996903946001346	6.013401682108793
3	3.566671852713265	3.5758685400051107
4	2.0217443191676145	2.702609867271025
5	1.4380924684804364	2.4030009992581256
6	1.0092785246837153	2.02376191225653
7	0.7671804601900437	1.7947034936872615
8	0.5922783245217783	1.583481381743011
9	0.43923933313504016	1.321115744103766
>10	5.568499602121147	43.86168781147457
>50	0.26431218560247105	5.27618131234365
>100	0.029597458283610035	1.7496026098306579
>500	0.002064938950019305	0.5106649742077387
>1k	0.002753251933359073	2.018736852994196
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3799	0.8739742477817065	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCG	1926	0.4430835486253138	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGC	1699	0.39086134429616204	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTC	1352	0.3110326883392649	No Hit
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	970	0.22315215065760868	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	705	0.16218790331300426	No Hit
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGC	545	0.12537930114267704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10352419360404526	0.0
2	0.0	0.0	0.0	0.6066517745197052	0.0
3	0.0	0.0	0.0	0.7640085487978541	0.0
4	0.0	0.0	0.0	1.0587074199240363	0.0
5	0.0	0.0	0.0	1.9849038720348946	0.0
6	0.0	0.0	0.0	2.362882205571442	0.0
7	0.0	0.0	0.0	2.8151679047393374	0.0
8	0.0	0.0	0.0	3.524883765335959	0.0
9	0.0	0.0	0.0	3.7749522063306196	0.0
10	0.0	0.0	0.0	4.815945486460186	0.0
11	0.0	0.0	0.0	5.717986293396767	0.0
12	0.0	0.0	0.0	6.848010380025812	0.0
13	0.0	0.0	0.0	7.164334304927062	0.0
14	0.0	0.0	0.0	7.32215118673234	0.0
15	0.0	0.0	0.0	7.633874036362298	0.0
16	0.0	0.0	0.0	8.116986939847843	0.0
17	0.0	0.0	0.0	8.63667839174015	0.0
18	0.0	0.0	0.0	9.266795650143438	0.0
19	0.0	0.0	0.0	9.65466629551326	0.0
20	0.0	0.0	0.0	10.056110112933393	0.0
21	0.0	0.0	0.0	10.534391887384082	0.0
22	0.0	0.0	0.0	10.999100489784462	0.0
23	0.0	0.0	0.0	11.433902102921452	0.0
24	0.0	0.0	0.0	11.786804576229464	0.0
25	0.0	0.0	0.0	12.12820436135925	0.0
26	0.0	0.0	0.0	12.460401995946453	0.0
27	0.0	0.0	0.0	12.865066566056488	0.0
28	0.0	0.0	0.0	13.215668501728855	0.0
29	0.0	0.0	0.0	13.614811781513339	0.0
30	0.0	0.0	0.0	14.112188018339886	0.0
31	0.0	0.0	0.0	14.512481566942194	0.0
32	0.0	0.0	0.0	14.878957212300515	0.0
33	0.0	0.0	0.0	15.257165599600627	0.0
34	0.0	0.0	0.0	15.675633395524533	0.0
35	0.0	0.0	0.0	16.13712124523501	0.0
36	0.0	0.0	0.0	16.52315146049632	0.0
37	0.0	0.0	0.0	16.91815377253664	0.0
38	0.0	0.0	0.0	17.42473215990577	0.0
39	0.0	0.0	0.0	18.31504022490056	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTA	20	7.02844E-4	45.000004	1
ACCGTCT	20	7.02844E-4	45.000004	35
CGCGTTT	20	7.02844E-4	45.000004	20
TTGCACG	40	6.7975634E-9	45.000004	1
CGTTTTA	80	0.0	45.000004	1
TAGCTAG	45	3.8380676E-10	45.000004	1
TGTAACG	20	7.02844E-4	45.000004	1
AATGTCG	20	7.02844E-4	45.000004	15
ATCGAAA	20	7.02844E-4	45.000004	17
CTATGAG	25	3.88679E-5	45.0	1
GTTAGAT	25	3.88679E-5	45.0	24
TACGCTC	25	3.88679E-5	45.0	20
TACCTAG	25	3.88679E-5	45.0	25
GCCACGA	25	3.88679E-5	45.0	43
AATCGCT	25	3.88679E-5	45.0	37
CGCATCG	30	2.1623746E-6	44.999996	21
CCGCTAG	30	2.1623746E-6	44.999996	10
CGTTTTT	1755	0.0	42.051285	1
TACGGGT	50	1.0786607E-9	40.5	4
TTCACGG	45	1.9235813E-8	40.000004	2
>>END_MODULE