SRR2936621.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936621.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086868
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	6569	0.6043972221097687	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	6563	0.6038451771512272	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	6370	0.5860877309848114	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3640	0.33490727484846367	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2770	0.25486075585995727	Illumina PCR Primer Index 2 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1386	0.12752238542306887	Illumina PCR Primer Index 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1373	0.12632628801289578	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	1307	0.1202537934689401	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1283	0.11804561363477442	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	25	3.8906655E-5	45.0	1
GCTATCG	20	7.033117E-4	45.0	1
CCAATCG	20	7.033117E-4	45.0	12
TAACGTA	20	7.033117E-4	45.0	11
TTACGCG	25	3.8906655E-5	45.0	1
GCTACGC	20	7.033117E-4	45.0	29
CGACTAG	20	7.033117E-4	45.0	2
TAATGCG	20	7.033117E-4	45.0	1
ATCGCGA	20	7.033117E-4	45.0	1
CGGACTA	20	7.033117E-4	45.0	6
CGAACTC	30	2.1653923E-6	44.999996	21
TTAGACG	55	6.184564E-11	40.909092	1
CGGTCTA	55	6.184564E-11	40.909092	31
TACGGCT	800	0.0	39.9375	7
CGAATAT	260	0.0	39.80769	14
CGTTTTT	1725	0.0	38.347828	1
CTACGAA	275	0.0	37.636364	11
GCTACGA	275	0.0	37.636364	10
TTTACGG	90	0.0	37.500004	2
GACGGTC	60	1.5643309E-10	37.499996	29