##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936621.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086868
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12705682750803	31.0	31.0	33.0	28.0	34.0
2	31.16117136579603	31.0	31.0	34.0	28.0	34.0
3	30.982512135788337	31.0	31.0	34.0	28.0	34.0
4	34.95163442110726	35.0	35.0	37.0	32.0	37.0
5	35.15746898427408	35.0	35.0	37.0	33.0	37.0
6	35.10467968511355	36.0	35.0	37.0	32.0	37.0
7	30.99103847017301	35.0	35.0	37.0	0.0	37.0
8	32.994979151102065	35.0	35.0	37.0	17.0	37.0
9	36.20374507299874	39.0	35.0	39.0	32.0	39.0
10	35.97772774614764	37.0	35.0	39.0	32.0	39.0
11	36.25207844926891	37.0	35.0	39.0	32.0	39.0
12	36.346387969836265	38.0	35.0	39.0	32.0	39.0
13	36.27773289856726	38.0	35.0	39.0	32.0	39.0
14	37.24508587979405	39.0	36.0	40.0	32.0	41.0
15	37.433115152898054	39.0	36.0	41.0	32.0	41.0
16	37.41480474169816	39.0	36.0	40.0	32.0	41.0
17	37.29768932381853	39.0	36.0	40.0	32.0	41.0
18	37.24337730064737	39.0	36.0	40.0	32.0	41.0
19	37.098033983887646	39.0	36.0	40.0	32.0	41.0
20	37.06449725265626	39.0	35.0	40.0	31.0	41.0
21	37.10209795485744	39.0	35.0	40.0	32.0	41.0
22	37.2195565606863	39.0	35.0	40.0	32.0	41.0
23	37.30089026450314	39.0	35.0	40.0	32.0	41.0
24	37.22178406209402	39.0	35.0	40.0	32.0	41.0
25	36.91822834051605	39.0	35.0	40.0	31.0	41.0
26	36.889071165955755	39.0	35.0	40.0	31.0	41.0
27	36.93231836800789	39.0	35.0	40.0	31.0	41.0
28	36.91711688999952	39.0	35.0	40.0	31.0	41.0
29	36.88817685312292	39.0	35.0	40.0	31.0	41.0
30	36.67607473952679	39.0	35.0	40.0	31.0	41.0
31	36.66110880070073	39.0	35.0	40.0	31.0	41.0
32	36.554780341310995	39.0	35.0	40.0	30.0	41.0
33	36.4720545641237	39.0	35.0	40.0	30.0	41.0
34	36.3906058509405	39.0	35.0	40.0	30.0	41.0
35	36.352184441900945	39.0	35.0	40.0	30.0	41.0
36	36.30243783053692	39.0	35.0	40.0	30.0	41.0
37	36.2396924005491	39.0	35.0	40.0	30.0	41.0
38	36.06322662917668	38.0	35.0	40.0	29.0	41.0
39	35.988135633766014	38.0	35.0	40.0	29.0	41.0
40	35.89144219905269	38.0	35.0	40.0	29.0	41.0
41	35.72335831030079	38.0	35.0	40.0	28.0	41.0
42	35.794331050320736	38.0	35.0	40.0	29.0	41.0
43	35.80449419800748	38.0	35.0	40.0	29.0	41.0
44	35.63374393210583	38.0	35.0	40.0	28.0	41.0
45	35.562324955744394	38.0	34.0	40.0	28.0	41.0
46	35.47099371772837	38.0	34.0	40.0	28.0	41.0
47	35.40525252376553	38.0	34.0	40.0	28.0	41.0
48	35.3185060191302	38.0	34.0	40.0	27.0	41.0
49	35.23539197032206	38.0	34.0	40.0	27.0	41.0
50	35.076691925790435	38.0	34.0	40.0	26.0	41.0
51	33.41477713945024	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	43.0
9	119.0
10	140.0
11	95.0
12	54.0
13	47.0
14	55.0
15	88.0
16	156.0
17	332.0
18	527.0
19	959.0
20	1713.0
21	2661.0
22	3695.0
23	5122.0
24	7083.0
25	9426.0
26	11928.0
27	14027.0
28	16302.0
29	19816.0
30	25023.0
31	31958.0
32	42287.0
33	58070.0
34	85080.0
35	90951.0
36	109538.0
37	162169.0
38	215702.0
39	171691.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73431916295263	21.26974020764251	23.06094208312325	10.934998546281609
2	32.89884328179687	25.65150505857197	24.59682316527858	16.852828494352583
3	33.07770584836429	24.397166905272766	26.157730285554454	16.367396960808488
4	29.236024981874525	25.14058744944188	26.785589418402232	18.83779815028136
5	27.017724323468904	29.60423896922165	24.32126072347332	19.056775983836125
6	26.69974642734904	35.69789523658807	24.52652944055764	13.075828895505252
7	68.39018169639736	17.98148441209052	9.974716340898802	3.653617550613322
8	78.58553200572655	7.835450119057697	8.933927579062038	4.645090296153718
9	72.3613171056651	7.934726204102062	11.193631609358267	8.510325080874587
10	42.2588575613598	24.0076071795287	19.106460030104852	14.62707522900665
11	30.8468921708984	26.342021294214202	24.929154230320517	17.881932304566885
12	27.859776900230752	23.03462794009944	29.441569721438114	19.66402543823169
13	24.760228473006844	24.111391631734488	31.71268268087753	19.41569721438114
14	20.941733494775814	26.856987233040257	30.975518646238548	21.225760625945377
15	20.022210608831983	25.928999657732128	33.99189229970889	20.056897433727002
16	24.450715266251283	25.851621356043236	29.217899505735744	20.479763871969734
17	24.874593786918005	25.567778239859855	28.096880209924297	21.460747763297842
18	24.68662247853465	25.603017109713416	29.544158076233728	20.166202335518204
19	23.911919386714853	27.151319203435925	27.23302185730006	21.70373955254916
20	24.092898125623353	28.00303256697225	29.16738739202921	18.73668191537519
21	24.079097001659814	27.890875432895257	29.74924277833187	18.280784787113063
22	22.840860159651402	24.242226286908807	30.214800693368467	22.702112860071324
23	22.246031716823016	26.521251890753984	30.263748679692476	20.968967712730525
24	23.253053728695665	25.708825726767188	29.31137911871543	21.726741425821718
25	22.747564561657903	27.545847333806865	27.722593728033214	21.98399437650202
26	21.190889786064176	26.4824247286699	28.37741105635643	23.94927442890949
27	22.11124073944582	26.28874895571495	28.98576460066908	22.61424570417015
28	19.637435272728613	26.526956355325577	30.11810081813063	23.71750755381518
29	21.937530592491452	26.05909825296172	28.600437219607166	23.40293393493966
30	22.26185700563454	25.545604434025105	29.983677870725796	22.20886068961456
31	23.30218573000585	26.141076929305125	28.454697350552227	22.102039990136795
32	25.140035404483342	26.845118266431616	26.445253701461446	21.569592627623592
33	23.26326656042868	24.820677395967127	28.069830006955765	23.846226036648424
34	21.572720882388662	26.269335374672913	30.032993887022158	22.124949855916263
35	21.422932683637754	27.24240662159526	27.978650581303345	23.35601011346364
36	22.617557973921397	27.24305067404689	27.21278020882021	22.926611143211503
37	21.96770905022505	27.225937280332108	28.314293916096528	22.492059753346314
38	21.794642955722317	26.780160976309908	29.020451425564097	22.404744642403678
39	22.703676987453857	26.466507432365294	27.615036968610724	23.214778611570125
40	22.810497686931626	25.567134187408225	29.8695885792939	21.752779546366256
41	19.77250227258508	26.87060434201761	27.73299057475241	25.6239028106449
42	20.224903116109775	26.55584670815591	29.708483458892893	23.51076671684142
43	21.722601088632658	24.567472774982797	28.67339916162772	25.036526974756825
44	22.01067654949819	24.925657945583087	28.918231100740844	24.145434404177877
45	21.13826150001656	24.769613237302046	28.27638682894335	25.815738433738044
46	22.725666778302426	26.420503685820172	28.126966660164804	22.726862875712598
47	20.275507237309405	25.344108024157485	31.293956579823863	23.086428158709246
48	20.494485070864172	26.940622044259282	28.899369564657345	23.665523320219197
49	21.353835056326986	24.88048226647578	30.772826138960756	22.992856538236474
50	21.207543142313508	24.932926537537217	30.03649017175959	23.823040148389683
51	20.508746232293156	24.962184920339915	28.05556884552678	26.47350000184015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	337.0
1	920.0
2	1503.0
3	3908.0
4	6313.0
5	4323.5
6	2334.0
7	2196.0
8	2058.0
9	2070.5
10	2083.0
11	2045.0
12	2007.0
13	2010.5
14	2014.0
15	2145.5
16	2277.0
17	2281.0
18	2285.0
19	2340.5
20	2396.0
21	3256.0
22	4116.0
23	4702.0
24	5288.0
25	7018.0
26	9950.5
27	11153.0
28	13746.5
29	16340.0
30	19528.5
31	22717.0
32	25744.0
33	28771.0
34	33159.5
35	37548.0
36	38661.0
37	39774.0
38	43407.0
39	47040.0
40	49655.0
41	52270.0
42	56439.0
43	60608.0
44	66746.0
45	72884.0
46	90281.5
47	107679.0
48	98195.0
49	88711.0
50	86522.0
51	84333.0
52	76751.5
53	69170.0
54	63941.5
55	58713.0
56	55538.5
57	52364.0
58	50155.5
59	47947.0
60	44615.5
61	41284.0
62	37188.5
63	33093.0
64	28234.5
65	23376.0
66	19948.5
67	16521.0
68	14355.0
69	12189.0
70	9931.5
71	7674.0
72	6060.0
73	4446.0
74	3706.0
75	2399.0
76	1832.0
77	1313.0
78	794.0
79	569.0
80	344.0
81	289.0
82	234.0
83	196.5
84	159.0
85	115.0
86	71.0
87	59.0
88	47.0
89	40.0
90	33.0
91	25.0
92	17.0
93	12.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1086868.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.21671221123493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15240877220205	22.104252834282466
2	9.285558695566207	5.611581096489081
3	3.9334211091218463	3.56565160979794
4	2.3691193259921537	2.863479874703191
5	1.6059226151421597	2.4262850747632214
6	1.1542619752411745	2.0926801153340486
7	0.8556246176851818	1.8097913983408738
8	0.6570310370692966	1.5882654208777727
9	0.5201129609769459	1.4144493293256277
>10	6.020116615725724	43.95050224141722
>50	0.4076292310794117	7.363180787861323
>100	0.032824883552250876	1.6912115707901916
>500	0.0029840803227665167	0.6085512311193246
>1k	0.0020888562259365616	1.1516396983043435
>5k	8.952240968299549E-4	1.7584777165934313
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	6569	0.6043972221097687	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	6563	0.6038451771512272	Illumina PCR Primer Index 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGC	6370	0.5860877309848114	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3640	0.33490727484846367	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	2770	0.25486075585995727	Illumina PCR Primer Index 2 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	1386	0.12752238542306887	Illumina PCR Primer Index 2 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1373	0.12632628801289578	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	1307	0.1202537934689401	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	1283	0.11804561363477442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8401498618047453E-4	0.0	0.0	0.0784823916059724	0.0
2	1.8401498618047453E-4	0.0	0.0	0.5555412432788527	0.0
3	1.8401498618047453E-4	0.0	0.0	0.7129660639562486	0.0
4	1.8401498618047453E-4	0.0	0.0	1.0623185152198795	0.0
5	1.8401498618047453E-4	0.0	0.0	2.2570358129966106	0.0
6	1.8401498618047453E-4	0.0	0.0	2.7984079023395667	0.0
7	1.8401498618047453E-4	0.0	0.0	3.2781349713120638	0.0
8	1.8401498618047453E-4	0.0	0.0	3.996529477360636	0.0
9	1.8401498618047453E-4	0.0	0.0	4.225168097689876	0.0
10	1.8401498618047453E-4	0.0	0.0	5.385566600543949	0.0
11	1.8401498618047453E-4	0.0	0.0	6.115738065708071	0.0
12	1.8401498618047453E-4	0.0	0.0	7.345878248324544	0.0
13	1.8401498618047453E-4	0.0	0.0	7.64297044351292	0.0
14	1.8401498618047453E-4	0.0	0.0	7.798739129314692	0.0
15	1.8401498618047453E-4	0.0	0.0	8.100063669185218	0.0
16	1.8401498618047453E-4	0.0	0.0	8.53535111899513	0.0
17	1.8401498618047453E-4	0.0	0.0	8.989592112381633	0.0
18	1.8401498618047453E-4	0.0	0.0	9.499957676553178	0.0
19	1.8401498618047453E-4	0.0	0.0	9.8456298280932	0.0
20	1.8401498618047453E-4	0.0	0.0	10.185689522554718	0.0
21	1.8401498618047453E-4	0.0	0.0	10.598987181516062	0.0
22	1.8401498618047453E-4	0.0	0.0	11.022497672210426	0.0
23	1.8401498618047453E-4	0.0	0.0	11.432759083899793	0.0
24	1.8401498618047453E-4	0.0	0.0	11.79204834441717	0.0
25	1.8401498618047453E-4	0.0	0.0	12.119871042297685	0.0
26	1.8401498618047453E-4	0.0	0.0	12.445117530371673	0.0
27	1.8401498618047453E-4	0.0	0.0	12.789501577008432	0.0
28	1.8401498618047453E-4	0.0	0.0	13.100946941118885	0.0
29	1.8401498618047453E-4	0.0	0.0	13.443398830400747	0.0
30	1.8401498618047453E-4	0.0	0.0	13.87132568076344	0.0
31	1.8401498618047453E-4	0.0	0.0	14.229418843870645	0.0
32	1.8401498618047453E-4	0.0	0.0	14.569846568304524	0.0
33	2.7602247927071183E-4	0.0	0.0	14.918462959623431	0.0
34	2.7602247927071183E-4	0.0	0.0	15.284284752150215	0.0
35	2.7602247927071183E-4	0.0	0.0	15.66666789343324	0.0
36	2.7602247927071183E-4	0.0	0.0	16.02411700408881	0.0
37	2.7602247927071183E-4	0.0	0.0	16.388374669233063	0.0
38	2.7602247927071183E-4	0.0	0.0	16.804156530507843	0.0
39	2.7602247927071183E-4	0.0	0.0	17.42575915382549	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	25	3.8906655E-5	45.0	1
GCTATCG	20	7.033117E-4	45.0	1
CCAATCG	20	7.033117E-4	45.0	12
TAACGTA	20	7.033117E-4	45.0	11
TTACGCG	25	3.8906655E-5	45.0	1
GCTACGC	20	7.033117E-4	45.0	29
CGACTAG	20	7.033117E-4	45.0	2
TAATGCG	20	7.033117E-4	45.0	1
ATCGCGA	20	7.033117E-4	45.0	1
CGGACTA	20	7.033117E-4	45.0	6
CGAACTC	30	2.1653923E-6	44.999996	21
TTAGACG	55	6.184564E-11	40.909092	1
CGGTCTA	55	6.184564E-11	40.909092	31
TACGGCT	800	0.0	39.9375	7
CGAATAT	260	0.0	39.80769	14
CGTTTTT	1725	0.0	38.347828	1
CTACGAA	275	0.0	37.636364	11
GCTACGA	275	0.0	37.636364	10
TTTACGG	90	0.0	37.500004	2
GACGGTC	60	1.5643309E-10	37.499996	29
>>END_MODULE