##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936620.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	159070
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.000050292324133	31.0	31.0	33.0	28.0	34.0
2	31.067919783743005	31.0	31.0	34.0	28.0	34.0
3	30.769334255359276	31.0	30.0	34.0	27.0	34.0
4	34.71741371723141	35.0	35.0	37.0	32.0	37.0
5	35.04025271892878	35.0	35.0	37.0	32.0	37.0
6	35.051926824668385	35.0	35.0	37.0	32.0	37.0
7	31.023649965424028	35.0	35.0	37.0	0.0	37.0
8	33.0991010247061	35.0	35.0	37.0	17.0	37.0
9	36.307229521594266	39.0	35.0	39.0	32.0	39.0
10	36.09092223549381	37.0	35.0	39.0	32.0	39.0
11	36.29372603256428	37.0	35.0	39.0	32.0	39.0
12	36.2322688124725	37.0	35.0	39.0	32.0	39.0
13	35.90917206261394	38.0	35.0	39.0	31.0	39.0
14	36.93207392971647	39.0	35.0	40.0	31.0	41.0
15	37.255736468221535	39.0	35.0	40.0	32.0	41.0
16	37.22752247438235	39.0	35.0	40.0	32.0	41.0
17	37.21716225561074	39.0	35.0	40.0	32.0	41.0
18	37.079612749104164	39.0	35.0	40.0	32.0	41.0
19	36.810498522662975	38.0	35.0	40.0	32.0	41.0
20	36.72873577670208	38.0	35.0	40.0	32.0	41.0
21	36.672125479348715	38.0	35.0	40.0	31.0	41.0
22	36.737379769912614	38.0	35.0	40.0	32.0	41.0
23	36.88756522285786	38.0	35.0	40.0	32.0	41.0
24	36.81365436600239	38.0	35.0	40.0	32.0	41.0
25	36.486087885836426	38.0	35.0	40.0	31.0	41.0
26	36.499509649839695	38.0	35.0	40.0	31.0	41.0
27	36.484145344816746	38.0	35.0	40.0	31.0	41.0
28	36.507330106242534	38.0	35.0	40.0	31.0	41.0
29	36.51886590809078	38.0	35.0	40.0	31.0	41.0
30	36.27868234110769	38.0	35.0	40.0	31.0	41.0
31	36.17261582950902	38.0	35.0	40.0	30.0	41.0
32	35.88988495630854	38.0	35.0	40.0	30.0	41.0
33	35.608449110454515	38.0	35.0	40.0	29.0	41.0
34	35.45559816433017	38.0	35.0	40.0	27.0	41.0
35	35.292915068837615	38.0	34.0	40.0	26.0	41.0
36	35.168152385742125	38.0	34.0	40.0	25.0	41.0
37	35.0711824982712	38.0	34.0	40.0	25.0	41.0
38	34.91826240020117	38.0	34.0	40.0	25.0	41.0
39	34.884308794870186	38.0	34.0	40.0	24.0	41.0
40	34.76028163701515	38.0	34.0	40.0	24.0	41.0
41	34.571653988809956	38.0	34.0	40.0	23.0	41.0
42	34.62261268623876	38.0	34.0	40.0	23.0	41.0
43	34.494970767586594	37.0	34.0	40.0	23.0	41.0
44	34.2792858489973	37.0	33.0	40.0	23.0	41.0
45	34.20635569246244	37.0	33.0	40.0	23.0	41.0
46	34.12859747281071	37.0	33.0	40.0	22.0	41.0
47	34.058628276859245	37.0	33.0	40.0	22.0	41.0
48	33.99029358144213	37.0	33.0	40.0	22.0	41.0
49	33.82788709373232	37.0	33.0	40.0	20.0	41.0
50	33.63323693971208	36.0	33.0	40.0	20.0	41.0
51	31.91024706104231	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	16.0
11	12.0
12	8.0
13	3.0
14	8.0
15	7.0
16	26.0
17	37.0
18	104.0
19	149.0
20	309.0
21	462.0
22	726.0
23	1065.0
24	1492.0
25	2262.0
26	2850.0
27	3227.0
28	3303.0
29	3582.0
30	4172.0
31	5089.0
32	6670.0
33	9222.0
34	14792.0
35	15608.0
36	14433.0
37	20858.0
38	27596.0
39	20970.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.52832086502798	19.8290060979443	24.76519771169925	16.877475325328472
2	34.31633871880304	23.799585088325895	25.020431256679448	16.863644936191612
3	29.28019111083171	22.239265732067643	32.45363676368894	16.026906393411704
4	27.475953982523414	24.52379455585591	30.803419878041115	17.196831583579556
5	24.875840824794114	28.13289746652417	29.74790972527818	17.24335198340353
6	24.546426101716225	31.26359464386748	29.532910039605202	14.657069214811088
7	65.17570880744327	17.596655560445086	13.22939586345634	3.9982397686553086
8	74.96322373797699	5.146162067014521	14.042245552272584	5.8483686427359025
9	70.34701703652479	6.471364807946187	16.45753441880933	6.724083736719683
10	42.25875400766958	22.276356321116488	22.767963789526625	12.696925881687307
11	32.24932419689445	23.885710693405418	27.763877538190734	16.1010875715094
12	29.789400892688754	21.696737285471805	31.626328031684164	16.887533790155278
13	22.74784685987301	28.264914817375995	31.481108945747156	17.506129377003834
14	16.43175960269064	32.09216068397561	32.56616583893883	18.90991387439492
15	15.35676117432577	25.744640724209468	42.51964543911485	16.378952662349906
16	16.75551643930345	23.90959954736908	40.099327340164706	19.23555667316276
17	18.547180486578235	22.554850066008676	32.51901678506318	26.37895266234991
18	20.89457471553404	23.56949770541271	35.22537247752562	20.31055510152763
19	22.407745017916643	26.984975168164958	31.03476456905765	19.572515244860753
20	26.394669013641792	25.798704972653546	30.237002577481615	17.569623436223047
21	22.163198591814925	26.367636889419753	32.28955805620167	19.17960646256365
22	18.753379015527756	24.93179103539322	31.30822908153643	25.006600867542588
23	18.29068963349469	26.073426793235683	32.104733765009115	23.531149808260515
24	20.736782548563525	23.136355063808388	35.0141447161627	21.112717671465393
25	17.394857609857294	25.698120324385492	32.819513421764	24.08750864399321
26	17.679637895266236	29.051361036021877	31.486138178160562	21.78286289055133
27	18.557867605456718	27.760105613880683	32.47815427170428	21.20387250895832
28	15.617652605771044	26.014961966429873	36.83724146602125	21.530143961777835
29	17.919783743006224	25.174451499339913	34.696674420066635	22.209090337587227
30	18.706229961652102	24.770226944112654	34.01961400641227	22.50392908782297
31	21.24473502231722	25.9684415666059	30.04463443766895	22.742188973407934
32	22.57559564971396	27.240208713145158	29.318539007983908	20.865656629156977
33	21.920538127868234	25.98478657194946	30.670773873137612	21.423901427044697
34	17.862576224303766	26.664361601810523	31.821210787703524	23.651851386182184
35	20.89897529389577	24.36411642673037	30.06475136732256	24.6721569120513
36	21.252907524989	24.265417740617337	31.67724900986987	22.804425724523796
37	18.842647890865656	27.56899478217137	32.518388131011505	21.06996919595147
38	20.03646193499717	28.389388319607722	28.55661029735337	23.01753944804174
39	19.453070975042433	24.983969321682277	32.80191110831709	22.761048594958194
40	22.991764631923054	23.85427799082165	31.56723455082668	21.586722826428616
41	18.151757088074433	24.41315144276105	31.124662098447224	26.31042937071729
42	21.516313572640975	23.837304331426417	29.677500471490536	24.96888162444207
43	22.943986923995723	25.12038725089583	28.888539636637955	23.047086188470484
44	19.70578990381593	26.578864650782673	29.906959200352045	23.80838624504935
45	18.957062928270574	27.01326460049035	28.753379015527752	25.276293455711325
46	21.478594329540453	26.196642987364054	29.41660904004526	22.90815364305023
47	18.474256616583894	25.458603130697178	31.72125479348714	24.345885459231784
48	19.870497265354874	22.781165524611808	33.059030615452315	24.289306594581003
49	18.880995788017856	23.21430816621613	34.720563274030305	23.18413277173571
50	18.233482114792228	25.12730244546426	32.57873891997234	24.06047651977117
51	17.8386873703401	24.933676997548247	29.431696737285474	27.79593889482618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	171.0
2	231.0
3	1807.0
4	3383.0
5	2288.5
6	1194.0
7	1116.0
8	1038.0
9	1010.0
10	982.0
11	981.5
12	981.0
13	944.0
14	907.0
15	831.5
16	756.0
17	786.0
18	816.0
19	718.5
20	621.0
21	702.0
22	783.0
23	844.5
24	906.0
25	1021.5
26	1231.5
27	1326.0
28	1526.5
29	1727.0
30	2024.0
31	2321.0
32	2570.5
33	2820.0
34	3368.5
35	3917.0
36	4505.5
37	5094.0
38	5786.0
39	6478.0
40	6991.5
41	7505.0
42	9504.0
43	11503.0
44	12547.0
45	13591.0
46	15081.0
47	16571.0
48	16496.5
49	16422.0
50	15276.5
51	14131.0
52	11943.0
53	9755.0
54	8666.0
55	7577.0
56	6553.0
57	5529.0
58	5297.0
59	5065.0
60	4830.0
61	4595.0
62	4337.5
63	4080.0
64	3129.5
65	2179.0
66	1618.0
67	1057.0
68	887.0
69	717.0
70	589.0
71	461.0
72	407.0
73	353.0
74	261.5
75	136.0
76	102.0
77	89.5
78	77.0
79	59.5
80	42.0
81	37.0
82	32.0
83	23.5
84	15.0
85	9.0
86	3.0
87	4.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	159070.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.66429873640536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44634989063937	30.71855158106494
2	8.932703585126953	7.086188470484692
3	3.4107839097220016	4.058590557616144
4	1.9241132278822075	3.0527440749355628
5	1.1887025707674264	2.3574526937826112
6	0.7369955938758044	1.753944804174263
7	0.6006910324278062	1.6678191990947382
8	0.4659714077408311	1.4785943295404538
9	0.35502583446920466	1.267366568177532
>10	4.832472184359844	40.832966618469854
>50	0.07449202776809205	1.7916640472747845
>100	0.028528861698418236	2.309674985855284
>500	0.0015849367610232352	0.40925378764066134
>1k	0.0015849367610232352	1.215188281888477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1933	1.215188281888477	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	651	0.40925378764066134	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	411	0.2583768152385742	No Hit
GGGGGAGGGCTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAAT	401	0.2520902747218206	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	330	0.20745583705286982	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	310	0.19488275601936253	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	299	0.18796756145093355	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	293	0.18419563714088136	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	280	0.17602313446910164	No Hit
ACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	177	0.11127176714653926	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2596341233419249	0.0
2	0.0	0.0	0.0	1.4402464323882567	0.0
3	0.0	0.0	0.0	1.8539007983906457	0.0
4	0.0	0.0	0.0	2.4699817690325014	0.0
5	0.0	0.0	0.0	3.9611491796064624	0.0
6	0.0	0.0	0.0	4.707990192996794	0.0
7	0.0	0.0	0.0	5.536556233104922	0.0
8	0.0	0.0	0.0	6.853586471364808	0.0
9	0.0	0.0	0.0	7.302445464261017	0.0
10	0.0	0.0	0.0	8.400075438486201	0.0
11	0.0	0.0	0.0	9.820204941220846	0.0
12	0.0	0.0	0.0	11.122147482240523	0.0
13	0.0	0.0	0.0	11.823725403910228	0.0
14	0.0	0.0	0.0	12.091532029923933	0.0
15	0.0	0.0	0.0	12.46620984472245	0.0
16	0.0	0.0	0.0	13.249512793109952	0.0
17	0.0	0.0	0.0	14.14031558433394	0.0
18	0.0	0.0	0.0	15.258062488212737	0.0
19	0.0	0.0	0.0	15.85151191299428	0.0
20	0.0	0.0	0.0	16.428616332432263	0.0
21	0.0	0.0	0.0	17.126422329791914	0.0
22	0.0	0.0	0.0	17.831772175771672	0.0
23	0.0	0.0	0.0	18.507575281322687	0.0
24	0.0	0.0	0.0	19.071477965675488	0.0
25	0.0	0.0	0.0	19.491418872194632	0.0
26	0.0	0.0	0.0	19.87929842207833	0.0
27	0.0	0.0	0.0	20.29546740428742	0.0
28	0.0	0.0	0.0	20.674545797447664	0.0
29	0.0	0.0	0.0	21.15106556861759	0.0
30	0.0	0.0	0.0	21.66593323693971	0.0
31	0.0	0.0	0.0	22.12862261897278	0.0
32	0.0	0.0	0.0	22.583139498334067	0.0
33	0.0	0.0	0.0	22.998679826491482	0.0
34	0.0	0.0	0.0	23.429307851889106	0.0
35	0.0	0.0	0.0	23.871251650216884	0.0
36	0.0	0.0	0.0	24.318853335009745	0.0
37	0.0	0.0	0.0	24.711762117306847	0.0
38	0.0	0.0	0.0	25.101527629345572	0.0
39	0.0	0.0	0.0	25.535298925001573	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACA	80	0.0	45.0	15
CGAACTT	20	7.0149486E-4	45.0	27
GTTCTCG	20	7.0149486E-4	45.0	43
CGAAAGG	20	7.0149486E-4	45.0	2
ACGTTGT	20	7.0149486E-4	45.0	38
TTAGCGG	20	7.0149486E-4	45.0	2
GCGAAAG	20	7.0149486E-4	45.0	1
CTTTTCG	25	3.875613E-5	45.0	12
CTTTTCA	20	7.0149486E-4	45.0	11
AGCCACT	25	3.875613E-5	45.0	36
GGCCGGT	20	7.0149486E-4	45.0	9
GCACGTT	20	7.0149486E-4	45.0	36
AAGACCC	20	7.0149486E-4	45.0	21
ACCGACT	20	7.0149486E-4	45.0	41
TCTAGTA	75	0.0	45.0	40
CGACAAT	20	7.0149486E-4	45.0	20
TATTGGG	45	3.8016879E-10	45.0	3
AAACGAC	20	7.0149486E-4	45.0	37
TCGGAGG	80	0.0	45.0	27
CCCCGCA	20	7.0149486E-4	45.0	25
>>END_MODULE