FastQCFastQC Report
Sat 14 Jan 2017
SRR2936618.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936618.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences809725
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.4124857204606502No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC32390.40001234987187007No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG30920.3818580382228534No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC25270.3120812621569051No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC14680.18129611905276483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAT302.164703E-645.00000419
CGACGGT406.8102963E-945.028
CAACCGA253.8897808E-545.025
AATAGCG253.8897808E-545.01
TGTCGCA207.0320506E-445.038
CGTTTTT18750.042.3599971
TAACGCC451.9270374E-840.012
GCCGATC451.9270374E-840.09
TTAGGCG800.039.3751
AATTGCG403.4565346E-739.3751
TACGTTG403.4565346E-739.3751
CTATACG356.246004E-638.57142618
CGCATCG356.246004E-638.57142621
TAGCGCG356.246004E-638.5714261
GGCCGAT1750.038.5714268
GCGTTAG356.246004E-638.5714261
GCGATAC356.246004E-638.5714269
CCGTTGT356.246004E-638.57142645
CGTTAGG1200.037.5000042
GCTACCG301.1396237E-437.5000041
Produced by FastQC (version 0.11.5)