##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936618.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	809725
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17198184568835	31.0	31.0	33.0	28.0	34.0
2	31.218607551946647	31.0	31.0	34.0	28.0	34.0
3	30.97816913149526	31.0	31.0	34.0	28.0	34.0
4	34.96309117292908	35.0	35.0	37.0	32.0	37.0
5	35.203802525548795	35.0	35.0	37.0	33.0	37.0
6	35.19181450492451	36.0	35.0	37.0	33.0	37.0
7	31.34831578622372	35.0	35.0	37.0	0.0	37.0
8	33.25875945537065	36.0	35.0	37.0	17.0	37.0
9	36.55224057550403	39.0	35.0	39.0	32.0	39.0
10	36.1406366358949	37.0	35.0	39.0	32.0	39.0
11	36.40028775201457	38.0	35.0	39.0	32.0	39.0
12	36.56411127234555	38.0	35.0	39.0	32.0	39.0
13	36.58503936521659	39.0	35.0	39.0	32.0	39.0
14	37.68529562505789	39.0	37.0	41.0	33.0	41.0
15	37.849543980981196	39.0	37.0	41.0	33.0	41.0
16	37.75751150081818	39.0	37.0	41.0	33.0	41.0
17	37.64236994041187	39.0	36.0	41.0	33.0	41.0
18	37.57794312884004	39.0	36.0	40.0	33.0	41.0
19	37.3895816480904	39.0	36.0	40.0	32.0	41.0
20	37.39833029732316	39.0	36.0	40.0	32.0	41.0
21	37.415049553860875	39.0	36.0	40.0	32.0	41.0
22	37.49301059001513	39.0	36.0	40.0	33.0	41.0
23	37.53498533452716	39.0	36.0	40.0	33.0	41.0
24	37.4843249251289	39.0	36.0	41.0	33.0	41.0
25	37.17446540492142	39.0	35.0	40.0	32.0	41.0
26	37.16391490938282	39.0	35.0	40.0	32.0	41.0
27	37.22090462811448	39.0	35.0	40.0	32.0	41.0
28	37.23039426965945	39.0	36.0	40.0	32.0	41.0
29	37.218444533637964	39.0	36.0	41.0	32.0	41.0
30	37.014390070703016	39.0	35.0	40.0	31.0	41.0
31	36.99270246071197	39.0	35.0	40.0	31.0	41.0
32	36.83384235388558	39.0	35.0	40.0	31.0	41.0
33	36.70922720676773	39.0	35.0	40.0	31.0	41.0
34	36.60810028095958	39.0	35.0	40.0	30.0	41.0
35	36.55120195127976	39.0	35.0	40.0	30.0	41.0
36	36.464098304980084	39.0	35.0	40.0	30.0	41.0
37	36.403252956250576	39.0	35.0	40.0	30.0	41.0
38	36.27676927351879	39.0	35.0	40.0	30.0	41.0
39	36.225448145975484	39.0	35.0	40.0	30.0	41.0
40	36.14348328136096	39.0	35.0	40.0	29.0	41.0
41	35.9802661397388	39.0	35.0	40.0	28.0	41.0
42	36.01964741115811	39.0	35.0	40.0	29.0	41.0
43	35.96251319892556	39.0	35.0	40.0	28.0	41.0
44	35.778464293432954	38.0	35.0	40.0	28.0	41.0
45	35.69074685850134	38.0	35.0	40.0	28.0	41.0
46	35.56808422612615	38.0	35.0	40.0	27.0	41.0
47	35.51040167958257	38.0	34.0	40.0	27.0	41.0
48	35.435693599678906	38.0	34.0	40.0	27.0	41.0
49	35.31909228441755	38.0	34.0	40.0	27.0	41.0
50	35.178032047917505	38.0	34.0	40.0	26.0	41.0
51	33.48662076630955	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	39.0
9	54.0
10	73.0
11	43.0
12	31.0
13	21.0
14	25.0
15	46.0
16	88.0
17	165.0
18	322.0
19	548.0
20	1043.0
21	1652.0
22	2465.0
23	3570.0
24	5072.0
25	7030.0
26	8942.0
27	10181.0
28	11438.0
29	13349.0
30	16594.0
31	21275.0
32	27430.0
33	37545.0
34	56707.0
35	65351.0
36	81831.0
37	126378.0
38	173161.0
39	137248.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61721572138689	21.550773410725863	25.50322640402606	11.328784463861187
2	32.09003056593288	24.570687579116367	26.758961375775726	16.58032047917503
3	32.192411003735835	24.689863842662632	27.905646978912596	15.212078174688937
4	28.381981536941552	25.996974281391832	27.450677699218872	18.170366482447744
5	26.832196115965296	30.381796288863505	24.90561610423292	17.880391490938283
6	26.093920775571956	35.7362067368551	24.79236777918429	13.377504708388651
7	71.0201611658279	17.119762882460094	8.600697767760659	3.259378183951341
8	83.19589984253913	6.210380067306802	7.483404859674581	3.110315230479484
9	77.25684646021797	8.034085646361419	8.956868072493748	5.752199820926858
10	38.259903053505816	32.48497946833802	16.969094445645126	12.286023032511038
11	28.341844453363795	27.625428386180495	26.643366575071788	17.389360585383926
12	26.42662632375189	24.23143659884529	29.843218376609343	19.49871870079348
13	24.392602426749825	25.568063231343974	31.19342986816512	18.845904473741086
14	20.995399672728396	28.469048133625613	29.615610238043782	20.919941955602212
15	20.432986507764983	28.027540214270275	32.93994874803174	18.599524529933
16	23.266170613479886	27.692982185309827	29.71675568865973	19.324091512550556
17	23.52959338046868	26.76451881811726	28.807187625428387	20.898700175985674
18	23.505881626478125	26.718453796041864	29.86655963444379	19.909104943036215
19	23.014603723486367	28.75173670073173	28.070764765815554	20.162894809966346
20	25.0115780048782	27.690635709654508	29.133100744079783	18.164685541387506
21	24.05927938497638	28.145234493192135	29.61091728673315	18.184568835098336
22	23.611720028404708	25.43493161258452	29.144215628762854	21.809132730247924
23	22.10565315384853	27.64000123498719	29.73688597980796	20.517459631356324
24	21.969557565840255	26.548149062953474	30.351292105344406	21.13100126586187
25	21.350334990274476	29.30068850535676	28.78890981506067	20.560066689308098
26	21.882984964031	28.753836178949644	28.699249745283893	20.663929111735467
27	21.68563401154713	29.11321745036895	28.47003612337522	20.731112414708697
28	20.106085399363984	28.36913767019667	30.68115718299423	20.843619747445118
29	22.534317206458983	26.83763005958813	29.558059835129207	21.069992898823674
30	22.743153539782025	27.666182963351755	28.732717898051806	20.85794559881441
31	22.726975207632222	27.85340702090216	27.732378276575382	21.68723949489024
32	23.24357034795764	29.0425761832721	27.3460742844793	20.367779184290963
33	23.093642903454878	28.282935564543514	27.436969341443085	21.186452190558523
34	22.41365895828831	28.495847355583688	27.719534409830498	21.37095927629751
35	21.983636419772147	27.70125659946278	28.323566642996077	21.991540337768996
36	23.173917070610393	28.02222976936614	27.863286918398224	20.940566241625245
37	22.356602550248542	28.902158140109297	28.411497730711044	20.329741578931117
38	21.561888295408934	28.628978974343138	27.31087714964957	22.49825558059835
39	22.507023989626106	28.284047053011825	28.013831856494487	21.19509710086758
40	23.203803760535983	26.85627836611195	28.957362067368553	20.982555805983512
41	21.33094569143845	27.780789774306093	28.576986013770107	22.31127852048535
42	21.693908425700084	26.8020624286023	28.73457037883232	22.769458766865295
43	22.5682793541017	27.05955725709346	28.458303745098647	21.913859643706196
44	22.49158664978851	27.651363117107657	27.74213467535274	22.11491555775109
45	22.153447157985738	27.643335700392107	26.866281762326714	23.33693537929544
46	22.778968168205253	28.715304578714996	26.7086973972645	21.797029855815246
47	21.529161134953227	27.301985241903115	29.100250084905372	22.06860353823829
48	21.88483744481151	26.638426626323753	28.78001790731421	22.696718021550527
49	21.208064466331162	26.38982370557905	30.010559140448915	22.391552687640864
50	21.35848590570873	26.843064003210966	28.98823674704375	22.810213344036555
51	21.400969464941802	25.786285467288277	28.11720028404705	24.69554478372287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	513.5
2	897.0
3	3598.5
4	6300.0
5	4439.0
6	2578.0
7	2395.0
8	2212.0
9	2249.5
10	2287.0
11	2199.5
12	2112.0
13	2100.5
14	2089.0
15	1997.5
16	1906.0
17	1923.0
18	1940.0
19	2019.0
20	2098.0
21	2510.5
22	2923.0
23	3589.5
24	4256.0
25	5170.5
26	7337.5
27	8590.0
28	10192.0
29	11794.0
30	13925.5
31	16057.0
32	19979.5
33	23902.0
34	26157.0
35	28412.0
36	30506.5
37	32601.0
38	36093.0
39	39585.0
40	42768.5
41	45952.0
42	49087.0
43	52222.0
44	53945.0
45	55668.0
46	57710.0
47	59752.0
48	68229.5
49	76707.0
50	72273.5
51	67840.0
52	65101.0
53	62362.0
54	55089.5
55	47817.0
56	42853.5
57	37890.0
58	34937.5
59	31985.0
60	28965.0
61	25945.0
62	21963.0
63	17981.0
64	14760.5
65	11540.0
66	8898.5
67	6257.0
68	5237.0
69	4217.0
70	3535.5
71	2854.0
72	2290.0
73	1726.0
74	1383.0
75	813.5
76	587.0
77	458.0
78	329.0
79	223.0
80	117.0
81	104.0
82	91.0
83	64.0
84	37.0
85	30.0
86	23.0
87	17.5
88	12.0
89	8.0
90	4.0
91	4.5
92	5.0
93	3.5
94	2.0
95	4.0
96	6.0
97	4.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	809725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.049129565912324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.8860179719654	23.35933433526933
2	9.079437913556546	5.819761641544608
3	3.890218903710126	3.7403438905430164
4	2.278422830977448	2.9208587406371596
5	1.5748644291714193	2.523651706963068
6	1.2126522282935723	2.33186690297838
7	0.8841684024015122	1.9835779380656238
8	0.6926512445198733	1.7759095583686269
9	0.588972571897054	1.6988452440747563
>10	6.699574118588629	46.986521957833894
>50	0.1825336071258712	3.495736656964015
>100	0.027437200013263028	1.4864482500659442
>500	0.0011432166672192855	0.20811008282904933
>1k	0.0019053611120321426	1.6690330938625513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3340	0.4124857204606502	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC	3239	0.40001234987187007	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	3092	0.3818580382228534	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC	2527	0.3120812621569051	No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC	1468	0.18129611905276483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07471672481398005	0.0
2	0.0	0.0	0.0	0.4326160116088796	0.0
3	0.0	0.0	0.0	0.5467288276884127	0.0
4	0.0	0.0	0.0	0.7849578560622433	0.0
5	0.0	0.0	0.0	1.603754361048504	0.0
6	0.0	0.0	0.0	1.9643706196548212	0.0
7	0.0	0.0	0.0	2.3288153385408625	0.0
8	0.0	0.0	0.0	2.905060359998765	0.0
9	0.0	0.0	0.0	3.085862484176727	0.0
10	0.0	0.0	0.0	3.9835746704127946	0.0
11	0.0	0.0	0.0	4.609713174225817	0.0
12	0.0	0.0	0.0	5.614745747012875	0.0
13	0.0	0.0	0.0	5.8521102843558	0.0
14	0.0	0.0	0.0	5.965235110685726	0.0
15	0.0	0.0	0.0	6.189508783846367	0.0
16	0.0	0.0	0.0	6.57754175800426	0.0
17	0.0	0.0	0.0	7.047083886504677	0.0
18	0.0	0.0	0.0	7.551576152397419	0.0
19	0.0	0.0	0.0	7.891444626262002	0.0
20	0.0	0.0	0.0	8.229707616783475	0.0
21	0.0	0.0	0.0	8.625768007656921	0.0
22	0.0	0.0	0.0	9.052456080768161	0.0
23	0.0	0.0	0.0	9.485689585970546	0.0
24	0.0	0.0	0.0	9.835808453487296	0.0
25	0.0	0.0	0.0	10.14961869770601	0.0
26	0.0	0.0	0.0	10.458118497020594	0.0
27	0.0	0.0	0.0	10.76735928864738	0.0
28	0.0	0.0	0.0	11.086850473926333	0.0
29	0.0	0.0	0.0	11.423878477260798	0.0
30	0.0	0.0	0.0	11.813887430917903	0.0
31	0.0	0.0	0.0	12.183766093426781	0.0
32	0.0	0.0	0.0	12.539318904566365	0.0
33	0.0	0.0	0.0	12.874988421995122	0.0
34	0.0	0.0	0.0	13.230911729290808	0.0
35	0.0	0.0	0.0	13.624749143227639	0.0
36	0.0	0.0	0.0	13.985118404396554	0.0
37	0.0	0.0	0.0	14.36747043749421	0.0
38	0.0	0.0	0.0	14.749451974435765	0.0
39	0.0	0.0	0.0	15.133779987032634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGCAT	30	2.164703E-6	45.000004	19
CGACGGT	40	6.8102963E-9	45.0	28
CAACCGA	25	3.8897808E-5	45.0	25
AATAGCG	25	3.8897808E-5	45.0	1
TGTCGCA	20	7.0320506E-4	45.0	38
CGTTTTT	1875	0.0	42.359997	1
TAACGCC	45	1.9270374E-8	40.0	12
GCCGATC	45	1.9270374E-8	40.0	9
TTAGGCG	80	0.0	39.375	1
AATTGCG	40	3.4565346E-7	39.375	1
TACGTTG	40	3.4565346E-7	39.375	1
CTATACG	35	6.246004E-6	38.571426	18
CGCATCG	35	6.246004E-6	38.571426	21
TAGCGCG	35	6.246004E-6	38.571426	1
GGCCGAT	175	0.0	38.571426	8
GCGTTAG	35	6.246004E-6	38.571426	1
GCGATAC	35	6.246004E-6	38.571426	9
CCGTTGT	35	6.246004E-6	38.571426	45
CGTTAGG	120	0.0	37.500004	2
GCTACCG	30	1.1396237E-4	37.500004	1
>>END_MODULE