##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936616.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	828044
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94689774939496	31.0	30.0	33.0	28.0	34.0
2	30.98278231591558	31.0	31.0	33.0	28.0	34.0
3	30.748809242020954	31.0	30.0	34.0	27.0	34.0
4	34.71718411098927	35.0	35.0	37.0	32.0	37.0
5	34.941994628304776	35.0	35.0	37.0	32.0	37.0
6	34.89334503963558	35.0	35.0	37.0	32.0	37.0
7	31.13393370400607	35.0	35.0	37.0	0.0	37.0
8	32.85915241219065	35.0	33.0	37.0	17.0	37.0
9	35.726446903787725	37.0	34.0	39.0	31.0	39.0
10	35.722206790943474	37.0	35.0	39.0	31.0	39.0
11	36.0304247117303	37.0	35.0	39.0	31.0	39.0
12	36.20802155441015	37.0	35.0	39.0	32.0	39.0
13	36.0098352261474	37.0	35.0	39.0	31.0	39.0
14	37.22292414412761	39.0	36.0	40.0	32.0	41.0
15	37.1245863746371	39.0	36.0	40.0	31.0	41.0
16	37.36692011535619	39.0	36.0	40.0	32.0	41.0
17	37.03661520402297	39.0	36.0	40.0	31.0	41.0
18	36.98994618643454	39.0	36.0	40.0	31.0	41.0
19	36.74203907038756	38.0	35.0	40.0	31.0	41.0
20	36.58611015839738	38.0	35.0	40.0	30.0	41.0
21	36.924427928950635	39.0	35.0	40.0	31.0	41.0
22	37.01072406780316	39.0	35.0	40.0	32.0	41.0
23	37.093146016395266	39.0	35.0	40.0	32.0	41.0
24	36.93328373854529	39.0	35.0	40.0	31.0	41.0
25	36.78025563858925	38.0	35.0	40.0	31.0	41.0
26	36.594936984025004	38.0	35.0	40.0	31.0	41.0
27	36.68842597736352	38.0	35.0	40.0	31.0	41.0
28	36.61927506267783	38.0	35.0	40.0	31.0	41.0
29	36.49009955992677	38.0	35.0	40.0	30.0	41.0
30	36.33891798020395	38.0	35.0	40.0	30.0	41.0
31	36.28997009820734	38.0	35.0	40.0	30.0	41.0
32	36.00893189250813	38.0	35.0	40.0	30.0	41.0
33	35.83547371878789	38.0	35.0	40.0	29.0	41.0
34	35.74596881325147	38.0	35.0	40.0	28.0	41.0
35	35.73367115757134	38.0	35.0	40.0	28.0	41.0
36	35.63416074508118	38.0	35.0	40.0	27.0	41.0
37	35.471733386148564	38.0	35.0	40.0	26.0	41.0
38	35.307379801073374	38.0	35.0	40.0	25.0	41.0
39	35.25843674973794	38.0	34.0	40.0	25.0	41.0
40	35.164230403215285	38.0	34.0	40.0	25.0	41.0
41	35.15319475776649	38.0	34.0	40.0	25.0	41.0
42	35.16338986817126	38.0	34.0	40.0	24.0	41.0
43	35.21739907541145	38.0	35.0	40.0	25.0	41.0
44	34.99091352633435	38.0	34.0	40.0	24.0	41.0
45	34.99706778866823	38.0	34.0	40.0	24.0	41.0
46	34.791186217157545	38.0	34.0	40.0	23.0	41.0
47	34.55382926511152	38.0	33.0	40.0	23.0	41.0
48	34.581820531276115	38.0	34.0	40.0	23.0	41.0
49	34.55480505866838	38.0	34.0	40.0	23.0	41.0
50	34.41089483167561	38.0	33.0	40.0	23.0	41.0
51	32.564907178845566	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	49.0
10	52.0
11	32.0
12	12.0
13	15.0
14	12.0
15	36.0
16	82.0
17	172.0
18	406.0
19	960.0
20	1735.0
21	2652.0
22	3795.0
23	5130.0
24	7394.0
25	9942.0
26	12592.0
27	13845.0
28	15058.0
29	17200.0
30	21102.0
31	27232.0
32	35766.0
33	46991.0
34	64653.0
35	74767.0
36	85642.0
37	118299.0
38	151194.0
39	111204.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.137123148045276	18.285380970093374	20.312809464231368	12.264686417629981
2	29.6398500562772	34.529566061706866	21.575665061276936	14.254918820738993
3	26.672012598364336	33.58710406693364	26.53337262271087	13.20751071199115
4	23.889068696832535	20.922197371154187	39.54451695803605	15.64421697397723
5	31.514267357773257	25.326914994855347	25.46664186927265	17.692175778098747
6	21.580133422861586	40.03108530464565	26.43035877320529	11.958422499287478
7	59.44406335895194	16.17571046949196	20.764597050398287	3.615629121157813
8	66.89765278173624	14.043939694025923	13.742023370738753	5.316384153499089
9	62.39040437464676	6.244716464342474	13.924260063474888	17.44061909753588
10	38.115607383182535	21.19537126046442	24.172628507663845	16.5163928486892
11	28.642922356782975	22.95735492316833	28.73265188806392	19.667070831984773
12	24.508600992217804	19.723951867292076	33.0709479206419	22.69649921984822
13	20.927028032326785	23.04901671891832	40.19629391674839	15.827661332006512
14	20.138543362430017	28.911748651037865	29.403993024525267	21.54571496200685
15	16.21387269275546	25.106395312326395	41.67991072938153	16.999821265536614
16	18.03805111805653	25.741989556110546	29.654945872441562	26.565013453391366
17	18.408321296935913	25.299380226171557	34.69875996927699	21.593538507615538
18	18.765910990237234	26.02397940206076	32.847771374468024	22.362338233233984
19	18.555052630053474	28.686156774277695	30.09477757220631	22.66401302346252
20	19.796894850998257	26.778166377632107	36.969533019984446	16.45540575138519
21	19.215283245817854	32.195994415755685	30.90246412026414	17.686258218162322
22	17.433010806189042	23.552854679219944	32.63956987792919	26.37456463666182
23	17.708237726497625	31.10027969528189	32.05047074793127	19.141011830289212
24	20.819062755119294	24.213568361101583	28.8535391839081	26.113829699871022
25	16.576051514170747	35.39038988266324	27.661211239982414	20.3723473631836
26	17.144499567655824	25.872779707358546	32.66855384496476	24.314166880020867
27	20.8142320939467	28.403804628739536	27.443589954157027	23.33837332315674
28	15.171295245180207	27.396974073841484	35.816937264203354	21.614793416774955
29	20.20556878619977	27.531628754027565	28.747143871581706	23.515658588190966
30	16.827125128616355	28.76489655139099	31.88526213582853	22.522716184164125
31	20.015723802116796	29.458458729246274	25.865412949070336	24.66040451956659
32	20.095550477993925	32.98701518276806	26.365024080845945	20.552410258392065
33	16.465187840259695	25.063523194419616	29.24518503847622	29.226103926844466
34	19.45017414533527	29.782596093927378	26.67479022853858	24.092439532198775
35	15.914130166995957	24.451599190381188	26.37456463666182	33.259706005961036
36	16.841013279487562	32.38994546183536	25.808169614175092	24.960871644501985
37	15.749525387539792	29.312693528363226	29.633207897164887	25.304573186932096
38	16.267613798300573	30.35515020940916	28.38424045099053	24.992995541299738
39	19.30646197545058	28.279052803957278	27.69405973595606	24.72042548463608
40	17.47588292409582	23.705020506156675	33.33095825825681	25.488138311490694
41	15.764258904116208	27.078029669920923	25.58233620435629	31.575375221606585
42	16.723145146876252	24.724893846220734	32.06351353309728	26.48844747380574
43	20.21003714778442	24.940824400635712	26.12614788586114	28.72299056571873
44	19.126278313712795	24.77851418523653	30.451038833685168	25.644168667365502
45	17.268285260203562	23.794025438261734	29.384428846776256	29.553260454758444
46	23.796682301906664	28.004308949765957	26.16636314012299	22.032645608204398
47	15.248706590471036	23.707435836742974	38.0007584138041	23.04309915898189
48	19.62902937525059	26.60112264565651	26.773939549106085	26.995908429986816
49	16.44864282574356	22.58623937858375	35.63880663346392	25.32631116220877
50	19.445222717633364	22.02552038297482	31.32526773939549	27.20398915999633
51	18.446362753670094	22.312220123568313	27.515566805628687	31.72585031713291
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	498.0
2	854.0
3	6643.0
4	12432.0
5	8627.0
6	4822.0
7	4522.0
8	4222.0
9	4032.0
10	3842.0
11	3670.5
12	3499.0
13	3426.5
14	3354.0
15	3185.0
16	3016.0
17	2912.5
18	2809.0
19	2788.0
20	2767.0
21	3373.0
22	3979.0
23	4115.0
24	4251.0
25	4763.0
26	5658.5
27	6042.0
28	7957.5
29	9873.0
30	12236.5
31	14600.0
32	16321.5
33	18043.0
34	19988.5
35	21934.0
36	24049.5
37	26165.0
38	29900.5
39	33636.0
40	36792.5
41	39949.0
42	44100.0
43	48251.0
44	54658.5
45	61066.0
46	115701.0
47	170336.0
48	125618.5
49	80901.0
50	76839.5
51	72778.0
52	60414.0
53	48050.0
54	40289.5
55	32529.0
56	28780.0
57	25031.0
58	22085.0
59	19139.0
60	17619.0
61	16099.0
62	13843.5
63	11588.0
64	9410.0
65	7232.0
66	5692.0
67	4152.0
68	3098.5
69	2045.0
70	1787.0
71	1529.0
72	1233.0
73	937.0
74	696.5
75	336.5
76	217.0
77	153.0
78	89.0
79	77.0
80	65.0
81	42.5
82	20.0
83	16.5
84	13.0
85	11.5
86	10.0
87	6.0
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	828044.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.596246555994576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56221971981986	28.384861162967525
2	9.415007132493738	6.891078446943768
3	3.566679416747779	3.9158123796447795
4	1.9074187292109808	2.792174643989076
5	1.236281556116892	2.2621632320141214
6	0.8466973916938831	1.8591567902848116
7	0.6583493483175482	1.6865180549705143
8	0.4834237457124051	1.4153195671290884
9	0.39436528975470814	1.2989060439290585
>10	3.751799498329716	29.65278372093141
>50	0.1363356333064833	3.1135831478453446
>100	0.03392081892592454	2.1671536727052056
>500	0.0026092937635324703	0.6821592442999407
>1k	0.002935455483974029	2.616197080763972
>5k	9.784851613246763E-4	2.2671798226129614
>10k+	9.784851613246763E-4	8.994952988968492
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	27014	3.2623870229118257	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	26617	3.214442710773824	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	21727	2.6238943824241225	No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	6873	0.8300283559810832	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6844	0.8265261266309519	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	5277	0.637284975194555	No Hit
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	4290	0.5180884107607808	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC	3597	0.4343972059455778	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA	2976	0.3594011912410452	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC	2480	0.299500992700871	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG	2344	0.28307674471404903	No Hit
GAATCTATCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	2312	0.27921221577597327	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTT	1455	0.17571530015313197	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	1286	0.15530575669891938	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT	1178	0.14226297153291373	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATG	922	0.11134674002830768	No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	919	0.11098444044036308	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2076652931486733E-4	0.0	0.0	0.18380665761722806	0.0
2	1.2076652931486733E-4	0.0	0.0	1.3934042152349393	0.0
3	1.2076652931486733E-4	0.0	0.0	1.6478592925013646	0.0
4	1.2076652931486733E-4	0.0	0.0	2.513030708513074	0.0
5	1.2076652931486733E-4	0.0	0.0	6.11259788127201	0.0
6	1.2076652931486733E-4	0.0	0.0	6.8282603339919135	0.0
7	1.2076652931486733E-4	0.0	0.0	7.993053509233809	0.0
8	1.2076652931486733E-4	0.0	0.0	9.293829796484244	0.0
9	1.2076652931486733E-4	0.0	0.0	9.600697547473322	0.0
10	1.2076652931486733E-4	0.0	0.0	14.055654047369464	0.0
11	1.2076652931486733E-4	0.0	0.0	15.172019844356097	0.0
12	1.2076652931486733E-4	0.0	0.0	19.81235296675056	0.0
13	1.2076652931486733E-4	0.0	0.0	20.32693914816121	0.0
14	1.2076652931486733E-4	0.0	0.0	20.59021018206762	0.0
15	1.2076652931486733E-4	0.0	0.0	21.28594615745057	0.0
16	1.2076652931486733E-4	0.0	0.0	22.010786866398405	0.0
17	1.2076652931486733E-4	0.0	0.0	22.853737241016177	0.0
18	1.2076652931486733E-4	0.0	0.0	23.691856954461358	0.0
19	1.2076652931486733E-4	0.0	0.0	24.43155194651492	0.0
20	1.2076652931486733E-4	0.0	0.0	24.970895266435118	0.0
21	1.2076652931486733E-4	0.0	0.0	25.60866330774693	0.0
22	1.2076652931486733E-4	0.0	0.0	26.30186318601427	0.0
23	1.2076652931486733E-4	0.0	0.0	27.05411789711658	0.0
24	1.2076652931486733E-4	0.0	0.0	27.62377361589481	0.0
25	1.2076652931486733E-4	0.0	0.0	28.143069691948735	0.0
26	1.2076652931486733E-4	0.0	0.0	28.618406751332056	0.0
27	1.2076652931486733E-4	0.0	0.0	29.132751399684075	0.0
28	1.2076652931486733E-4	0.0	0.0	29.62886030210955	0.0
29	1.2076652931486733E-4	0.0	0.0	30.118930878069282	0.0
30	1.2076652931486733E-4	0.0	0.0	30.641366883885397	0.0
31	1.2076652931486733E-4	0.0	0.0	31.184453966214356	0.0
32	1.2076652931486733E-4	0.0	0.0	31.70616537285458	0.0
33	1.2076652931486733E-4	0.0	0.0	32.192250653346925	0.0
34	1.2076652931486733E-4	0.0	0.0	32.65237113003657	0.0
35	1.2076652931486733E-4	0.0	0.0	33.133746515885626	0.0
36	1.2076652931486733E-4	0.0	0.0	33.64169053818396	0.0
37	1.2076652931486733E-4	0.0	0.0	34.1334518455541	0.0
38	1.2076652931486733E-4	0.0	0.0	34.62642081821739	0.0
39	1.2076652931486733E-4	0.0	0.0	35.117940592528896	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.0321443E-4	45.0	31
CTCGTCC	25	3.889857E-5	45.0	37
CGACGGT	20	7.0321443E-4	45.0	28
CGACGCG	25	3.889857E-5	45.0	15
CGACCGG	20	7.0321443E-4	45.0	2
TAGCACG	20	7.0321443E-4	45.0	1
CGCTCAC	20	7.0321443E-4	45.0	34
ACATGCG	25	3.889857E-5	45.0	1
TATCGCG	20	7.0321443E-4	45.0	1
TACCGAA	25	3.889857E-5	45.0	29
ACTACGG	60	0.0	44.999996	2
CGGTCGG	30	2.1647647E-6	44.999996	2
TTCGCGG	65	0.0	44.999996	2
CGTTTTT	3830	0.0	43.53133	1
ACGGCTG	3165	0.0	43.151657	8
TACGGCT	2925	0.0	43.0	7
CGGCTGT	3145	0.0	42.782196	9
TGATACC	3025	0.0	41.876034	4
GATACCT	3055	0.0	41.61211	5
ATGATAC	8290	0.0	40.765984	3
>>END_MODULE