##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936615.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507559
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170778175542154	31.0	31.0	33.0	28.0	34.0
2	31.200626922190327	31.0	31.0	34.0	28.0	34.0
3	30.99887500763458	31.0	31.0	34.0	28.0	34.0
4	34.96032973506528	35.0	35.0	37.0	32.0	37.0
5	35.20206911905808	35.0	35.0	37.0	33.0	37.0
6	35.18562571050853	36.0	35.0	37.0	32.0	37.0
7	30.970797483642297	35.0	35.0	37.0	0.0	37.0
8	33.03743998234688	36.0	35.0	37.0	17.0	37.0
9	36.269282979909725	39.0	35.0	39.0	32.0	39.0
10	36.13284169919162	37.0	35.0	39.0	32.0	39.0
11	36.40693791263676	38.0	35.0	39.0	32.0	39.0
12	36.477089363010016	38.0	35.0	39.0	32.0	39.0
13	36.38036563236983	38.0	35.0	39.0	32.0	39.0
14	37.44360557097796	39.0	36.0	40.0	32.0	41.0
15	37.645595881464025	39.0	36.0	41.0	33.0	41.0
16	37.591621860709786	39.0	36.0	41.0	33.0	41.0
17	37.50094668797125	39.0	36.0	41.0	32.0	41.0
18	37.41143788209843	39.0	36.0	40.0	32.0	41.0
19	37.238577190040964	39.0	36.0	40.0	32.0	41.0
20	37.23888257325749	39.0	35.0	40.0	32.0	41.0
21	37.259299904050565	39.0	35.0	40.0	32.0	41.0
22	37.342381870876096	39.0	35.0	40.0	32.0	41.0
23	37.396679006775564	39.0	35.0	40.0	33.0	41.0
24	37.3246144783168	39.0	35.0	41.0	32.0	41.0
25	37.01045395707691	39.0	35.0	40.0	32.0	41.0
26	36.98139920679172	39.0	35.0	40.0	32.0	41.0
27	36.98726453476345	39.0	35.0	40.0	32.0	41.0
28	37.0122488223044	39.0	35.0	40.0	32.0	41.0
29	37.04544102262003	39.0	35.0	40.0	32.0	41.0
30	36.83319377648707	39.0	35.0	40.0	31.0	41.0
31	36.784618142915406	39.0	35.0	40.0	31.0	41.0
32	36.65646949418688	39.0	35.0	40.0	31.0	41.0
33	36.54285511635101	39.0	35.0	40.0	30.0	41.0
34	36.49414156777833	39.0	35.0	40.0	30.0	41.0
35	36.45318475290557	39.0	35.0	40.0	30.0	41.0
36	36.37433283618259	39.0	35.0	40.0	30.0	41.0
37	36.27481731187901	39.0	35.0	40.0	30.0	41.0
38	36.176688818442784	39.0	35.0	40.0	30.0	41.0
39	36.137398804868006	39.0	35.0	40.0	30.0	41.0
40	36.04000717158005	38.0	35.0	40.0	30.0	41.0
41	35.88197628256025	38.0	35.0	40.0	29.0	41.0
42	35.89668984295422	38.0	35.0	40.0	29.0	41.0
43	35.84630161222636	38.0	35.0	40.0	29.0	41.0
44	35.660439476001805	38.0	35.0	40.0	28.0	41.0
45	35.591781448068105	38.0	35.0	40.0	28.0	41.0
46	35.50062948346892	38.0	34.0	40.0	28.0	41.0
47	35.442862800186774	38.0	34.0	40.0	28.0	41.0
48	35.35814358527777	38.0	34.0	40.0	27.0	41.0
49	35.27328842558205	38.0	34.0	40.0	27.0	41.0
50	35.10195070917864	38.0	34.0	40.0	27.0	41.0
51	33.40703642335177	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	43.0
10	42.0
11	41.0
12	25.0
13	15.0
14	30.0
15	34.0
16	75.0
17	118.0
18	243.0
19	415.0
20	684.0
21	1053.0
22	1507.0
23	2125.0
24	3167.0
25	4154.0
26	5563.0
27	6519.0
28	7583.0
29	8735.0
30	10887.0
31	13772.0
32	18202.0
33	26057.0
34	40582.0
35	45057.0
36	50753.0
37	75635.0
38	101374.0
39	83045.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.159514460387854	21.074003219330166	23.24202703528063	12.524455285001348
2	32.70910376921698	24.7027439174559	24.35618322204906	18.231969091278057
3	33.46408988905723	23.986176976469732	26.44520144456112	16.104531689911912
4	29.40761566635603	24.937987504900907	26.905049462230007	18.749347366513057
5	26.272413650432757	29.851307926763194	24.05887788414746	19.81740053865659
6	26.92455458380208	35.28240066672052	23.803538110840318	13.989506638637083
7	67.5371730183092	18.366928770842406	10.177536010591872	3.918362200256522
8	76.89825222289429	7.665709799254865	10.285897797103392	5.15014018074746
9	70.09194990139078	9.131746260040705	12.985485431250357	7.790818407318165
10	40.771614728534026	25.871869083200178	19.670816594721007	13.68569959354479
11	31.955496799386868	26.56262621685361	24.463559901410477	17.018317082349046
12	31.450924917103233	22.38045232179904	27.98984945592532	18.178773305172406
13	24.645213659889787	25.89708782624286	29.634781375170178	19.82291713869718
14	19.49369432913218	29.9980888921288	28.59963078184014	21.908585996898882
15	18.9755279681771	27.321946808154323	34.865897363656245	18.836627860012335
16	20.76192127417699	25.20830090688964	33.36518513118672	20.664592687746648
17	20.86910093210839	25.334197600673026	29.759495940373437	24.037205526845153
18	21.423716257617343	27.27210038635902	29.575871967593915	21.72831138842972
19	24.36268492923975	27.878926390823526	26.27930940048349	21.479079279453224
20	26.86761539052603	26.063373913180538	28.103530821047407	18.96547987524603
21	24.697621360275356	27.349726829787276	27.903751091006168	20.048900718931197
22	21.921392389850244	26.010769191364947	27.54083761690759	24.527000801877218
23	22.471279201038698	28.001867763156596	27.93330430550931	21.593548730295396
24	23.150215048890868	24.754166510691366	29.357769244560732	22.73784919585703
25	21.20876587746449	27.608021924544733	28.074371649404306	23.10884054858647
26	21.213691413215017	28.202041536057877	28.77951134744926	21.804755703277845
27	22.697853845562783	28.178595985885384	28.048955884931605	21.07459428362023
28	19.44522705734703	29.29708664411428	30.485322888570586	20.772363409968104
29	21.556114658591415	26.25015022884039	30.690225175792367	21.50350993677582
30	22.835571825147422	26.218626800037043	29.407418644926008	21.53838272988953
31	23.402599500747694	28.51983710268166	26.061403698880326	22.01615969769032
32	23.109234591446512	28.28479053666667	28.211301543268863	20.394673328617955
33	22.66573935246937	27.61747895318574	27.413758794544087	22.303022899800812
34	20.57731219424737	28.231003686270956	29.012587699164037	22.179096420317638
35	21.476518000862953	25.12397573484068	29.035048142186426	24.36445812210994
36	22.090436776808215	28.018220541848336	28.022555013308796	21.868787668034653
37	22.055366962264486	29.001751520512887	28.615786539101855	20.32709497812077
38	20.928798425404732	27.755393954200397	27.574134238581134	23.74167338181374
39	21.717278188348548	25.50225688048089	29.496866374155516	23.283598557015047
40	23.020968990797126	25.206330692589436	30.42661050242435	21.34608981418909
41	18.788751652517245	27.116847499502523	29.077210728210908	25.017190119769328
42	21.091144083741987	27.11901473523275	28.033194170529928	23.756647010495332
43	23.596271566458284	26.000918119863897	27.504585673783737	22.89822463989408
44	21.84613020358224	27.009273798711085	28.397683816068675	22.746912181637995
45	20.672079502087442	26.279703443343532	28.20893728610861	24.839279768460415
46	21.570103180122903	28.111608699678264	27.610189160274963	22.70809895992387
47	20.838365589025116	26.04465687732855	30.6001863822728	22.516791151373535
48	21.251125484918994	24.82332103262872	29.594786024875923	24.33076745757636
49	20.410238021589606	24.88183639734494	30.528076538885134	24.17984904218032
50	19.11600424778203	26.014118555675303	30.043994885323677	24.82588231121899
51	19.62491060152613	25.208891971179703	28.0065962774771	27.159601149817064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	359.0
1	573.0
2	787.0
3	2798.5
4	4810.0
5	3306.0
6	1802.0
7	1686.0
8	1570.0
9	1564.5
10	1559.0
11	1578.0
12	1597.0
13	1539.5
14	1482.0
15	1444.5
16	1407.0
17	1377.5
18	1348.0
19	1447.5
20	1547.0
21	1821.5
22	2096.0
23	2300.5
24	2505.0
25	3097.5
26	4680.5
27	5671.0
28	6457.0
29	7243.0
30	8513.5
31	9784.0
32	10343.5
33	10903.0
34	12895.5
35	14888.0
36	15814.0
37	16740.0
38	18824.5
39	20909.0
40	24667.0
41	28425.0
42	30547.0
43	32669.0
44	40758.0
45	48847.0
46	47381.0
47	45915.0
48	44108.0
49	42301.0
50	36629.0
51	30957.0
52	28302.5
53	25648.0
54	24544.5
55	23441.0
56	22300.5
57	21160.0
58	21727.0
59	22294.0
60	22381.0
61	22468.0
62	19850.5
63	17233.0
64	14599.0
65	11965.0
66	9777.0
67	7589.0
68	6507.5
69	5426.0
70	4551.5
71	3677.0
72	2885.5
73	2094.0
74	1660.0
75	943.0
76	660.0
77	575.0
78	490.0
79	321.0
80	152.0
81	123.0
82	94.0
83	79.0
84	64.0
85	46.0
86	28.0
87	22.5
88	17.0
89	12.5
90	8.0
91	8.0
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.29442012940149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75180881692918	23.08020090420279
2	9.675893099794962	6.056029275843209
3	3.7952175343908463	3.563073960110952
4	2.1380327262951235	2.6763397754835707
5	1.4454452782398592	2.2617185905648896
6	1.020504549898628	1.9161658877096062
7	0.766111457810017	1.6782509678658444
8	0.6073727180798495	1.5205901611781873
9	0.48027057168854087	1.3526810141588133
>10	5.91117901573161	43.998128880563755
>50	0.3535330186655034	6.72345258347276
>100	0.048351762765974714	2.5760723656030984
>500	0.0031397248549334227	0.6571352498723595
>1k	0.0031397248549334227	1.940160383370157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2658	0.5236829609956675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	2077	0.40921351015349944	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	1907	0.37571986704993904	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	1673	0.32961685242503824	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1558	0.3069593879726298	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	892	0.1757431155786815	RNA PCR Primer, Index 10 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	698	0.13752095815461848	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	614	0.12097115803285922	TruSeq Adapter, Index 10 (95% over 24bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	611	0.12038009374279642	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	529	0.10422433648107905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13830904387470225	0.0
2	0.0	0.0	0.0	0.8399023561792817	0.0
3	0.0	0.0	0.0	1.0434254933909162	0.0
4	0.0	0.0	0.0	1.408703224649745	0.0
5	0.0	0.0	0.0	2.602456069146641	0.0
6	0.0	0.0	0.0	3.127321158722434	0.0
7	0.0	0.0	0.0	3.6829215913814948	0.0
8	0.0	0.0	0.0	4.576216755096452	0.0
9	0.0	0.0	0.0	4.875689328728286	0.0
10	0.0	0.0	0.0	6.012108937089087	0.0
11	0.0	0.0	0.0	7.008052265844956	0.0
12	0.0	0.0	0.0	8.174616152998961	0.0
13	0.0	0.0	0.0	8.544031334288231	0.0
14	0.0	0.0	0.0	8.708544228355718	0.0
15	0.0	0.0	0.0	9.057469180922809	0.0
16	0.0	0.0	0.0	9.684785414109493	0.0
17	0.0	0.0	0.0	10.339684647499109	0.0
18	0.0	0.0	0.0	11.10885631030087	0.0
19	0.0	0.0	0.0	11.543485584927073	0.0
20	1.9702143002094338E-4	0.0	0.0	11.970234002352436	0.0
21	1.9702143002094338E-4	0.0	0.0	12.513225063490156	0.0
22	1.9702143002094338E-4	0.0	0.0	13.072174860459572	0.0
23	1.9702143002094338E-4	0.0	0.0	13.59585782145524	0.0
24	1.9702143002094338E-4	0.0	0.0	14.046445831913138	0.0
25	1.9702143002094338E-4	0.0	0.0	14.46235807068735	0.0
26	1.9702143002094338E-4	0.0	0.0	14.816208559004963	0.0
27	1.9702143002094338E-4	0.0	0.0	15.180698204543708	0.0
28	1.9702143002094338E-4	0.0	0.0	15.542626571492182	0.0
29	1.9702143002094338E-4	0.0	0.0	15.936472410104047	0.0
30	1.9702143002094338E-4	0.0	0.0	16.40892979929427	0.0
31	1.9702143002094338E-4	0.0	0.0	16.83548119528961	0.0
32	1.9702143002094338E-4	0.0	0.0	17.242921512572924	0.0
33	1.9702143002094338E-4	0.0	0.0	17.639722672635102	0.0
34	1.9702143002094338E-4	0.0	0.0	18.04637490419833	0.0
35	1.9702143002094338E-4	0.0	0.0	18.475881621643985	0.0
36	1.9702143002094338E-4	0.0	0.0	18.883321938927295	0.0
37	1.9702143002094338E-4	0.0	0.0	19.296081834821173	0.0
38	1.9702143002094338E-4	0.0	0.0	19.683228944812328	0.0
39	1.9702143002094338E-4	0.0	0.0	20.065056476192915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	25	3.887716E-5	45.000004	1
TCACACC	50	2.1827873E-11	45.000004	16
ATTCGTA	35	1.2101373E-7	45.000004	32
TTCGTAC	35	1.2101373E-7	45.000004	33
TATTGCG	25	3.887716E-5	45.000004	1
GAAACGT	35	1.2101373E-7	45.000004	20
CGGGACG	25	3.887716E-5	45.000004	6
CGTACAT	35	1.2101373E-7	45.000004	35
CGTAATG	25	3.887716E-5	45.000004	24
TAAGGCG	25	3.887716E-5	45.000004	5
CCTGCGT	25	3.887716E-5	45.000004	23
TGCGTTC	25	3.887716E-5	45.000004	25
CGAAATC	20	7.0295593E-4	45.0	22
CACGGGC	40	6.8030204E-9	45.0	4
CACGATA	20	7.0295593E-4	45.0	18
CGTTCGA	20	7.0295593E-4	45.0	14
CCGTCAA	20	7.0295593E-4	45.0	22
AACGGTC	20	7.0295593E-4	45.0	30
ATCCGCA	20	7.0295593E-4	45.0	11
CTAACGG	20	7.0295593E-4	45.0	2
>>END_MODULE