##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936614.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	792246
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22869916667298	31.0	31.0	33.0	30.0	34.0
2	31.270529608227747	31.0	31.0	34.0	28.0	34.0
3	31.05660741739308	31.0	31.0	34.0	28.0	34.0
4	35.009000992116086	35.0	35.0	37.0	32.0	37.0
5	35.221351701365485	35.0	35.0	37.0	33.0	37.0
6	35.18264402723396	36.0	35.0	37.0	32.0	37.0
7	30.779547009388498	35.0	35.0	37.0	0.0	37.0
8	32.918815620400736	35.0	35.0	37.0	17.0	37.0
9	36.27804621291872	39.0	35.0	39.0	32.0	39.0
10	36.15670006538373	37.0	35.0	39.0	32.0	39.0
11	36.37352160818735	38.0	35.0	39.0	32.0	39.0
12	36.44177313612186	38.0	35.0	39.0	32.0	39.0
13	36.367709019673185	38.0	35.0	39.0	32.0	39.0
14	37.376525725595336	39.0	36.0	41.0	32.0	41.0
15	37.5289947819238	39.0	36.0	41.0	32.0	41.0
16	37.53786955061938	39.0	36.0	41.0	33.0	41.0
17	37.40457509409956	39.0	36.0	41.0	32.0	41.0
18	37.328886482229	39.0	36.0	40.0	32.0	41.0
19	37.16687745977891	39.0	36.0	40.0	32.0	41.0
20	37.10604029556476	39.0	35.0	40.0	32.0	41.0
21	37.135054011001635	39.0	35.0	40.0	32.0	41.0
22	37.22986673331263	39.0	35.0	40.0	32.0	41.0
23	37.303188656048754	39.0	35.0	40.0	32.0	41.0
24	37.23967681755414	39.0	35.0	41.0	32.0	41.0
25	36.9421846244727	39.0	35.0	40.0	31.0	41.0
26	36.905549538905845	39.0	35.0	40.0	31.0	41.0
27	36.9163504769983	39.0	35.0	40.0	31.0	41.0
28	36.89157534402193	39.0	35.0	40.0	31.0	41.0
29	36.866586893464905	39.0	35.0	40.0	31.0	41.0
30	36.63650810480583	39.0	35.0	40.0	31.0	41.0
31	36.584658048131516	39.0	35.0	40.0	31.0	41.0
32	36.44803003107621	39.0	35.0	40.0	30.0	41.0
33	36.27479722207496	39.0	35.0	40.0	30.0	41.0
34	36.17905423315485	39.0	35.0	40.0	30.0	41.0
35	36.08267760266382	39.0	35.0	40.0	30.0	41.0
36	36.01813704329211	38.0	35.0	40.0	29.0	41.0
37	35.96612542063955	38.0	35.0	40.0	29.0	41.0
38	35.74454651711716	38.0	35.0	40.0	28.0	41.0
39	35.69882460750827	38.0	35.0	40.0	28.0	41.0
40	35.559793296526585	38.0	35.0	40.0	27.0	41.0
41	35.39441915768587	38.0	34.0	40.0	26.0	41.0
42	35.41757358194298	38.0	34.0	40.0	27.0	41.0
43	35.39912476680223	38.0	34.0	40.0	27.0	41.0
44	35.20670978458711	38.0	34.0	40.0	26.0	41.0
45	35.122631101955704	38.0	34.0	40.0	26.0	41.0
46	35.033906135215574	38.0	34.0	40.0	26.0	41.0
47	34.93321013927492	37.0	34.0	40.0	25.0	41.0
48	34.84046116988915	37.0	34.0	40.0	25.0	41.0
49	34.75040454606271	37.0	34.0	40.0	25.0	41.0
50	34.59640818634616	37.0	34.0	40.0	24.0	41.0
51	32.91035865122702	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	43.0
9	49.0
10	85.0
11	72.0
12	47.0
13	42.0
14	59.0
15	87.0
16	136.0
17	257.0
18	474.0
19	837.0
20	1459.0
21	2010.0
22	3051.0
23	4308.0
24	5954.0
25	7923.0
26	9914.0
27	11410.0
28	12600.0
29	15077.0
30	18044.0
31	22911.0
32	30348.0
33	42053.0
34	63368.0
35	67205.0
36	79667.0
37	117099.0
38	152602.0
39	123046.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59933656970183	20.662016595855327	23.063669617770238	12.6749772166726
2	34.06618651277507	25.275608838668795	24.856168412336572	15.802036236219557
3	32.511745089277824	24.326030046223014	27.444000979493744	15.718223885005415
4	28.906173082603132	24.34849781507259	28.218129217440037	18.52719988488424
5	26.955642565566755	28.43447616018257	25.613887605617446	18.995993668633226
6	26.62178161833572	34.44182741219268	25.409405664402218	13.526985305069386
7	67.62179928961459	18.41763795588744	10.348553353377612	3.612009401120359
8	79.48389767824641	7.042130853295567	9.642585762503062	3.8313857059549683
9	73.19582553903712	7.394420419920075	11.6112924520919	7.798461588950907
10	43.41972064232574	24.018297347036146	18.44187285262406	14.120109158014053
11	31.566962786811164	25.807766779510406	25.147239620017015	17.478030813661412
12	28.827409668209114	22.562183968110915	29.007656712687723	19.602749650992244
13	25.839575081477218	23.460011158150373	31.202555771818346	19.49785798855406
14	21.573854585570643	25.949768127576533	30.63985176321496	21.83652552363786
15	20.19044589685527	25.623354362155187	33.996258737816284	20.189941003173256
16	25.047523117819466	25.17096962307162	29.16341641358871	20.618090845520204
17	24.569262577532736	25.572864993953896	28.94214675744655	20.915725671066816
18	24.83609888847656	25.121237595393346	29.374840642931616	20.667822873198478
19	25.262607826356962	26.371859245739326	26.962711077114935	21.40282185078877
20	25.354372253062813	27.193447489794835	28.458206163237175	18.993974093905173
21	25.304514001964034	26.987703314374578	29.28888249356892	18.418900190092472
22	24.110440444003505	23.259063472709236	30.724547678372627	21.905948404914636
23	23.300843424895803	25.474536949381882	30.57610893586083	20.64851068986148
24	23.56035877745044	25.031997637097568	29.17174715934192	22.23589642611007
25	23.74224672639559	27.357916606710543	27.68710728738296	21.212729379510908
26	22.312009148673518	26.276939233520903	27.95545827937282	23.45559333843276
27	21.925639258513137	26.954127884520716	28.700681354023878	22.419551502942266
28	20.33004899993184	27.46646874834332	29.951807898051868	22.251674353672975
29	22.614566687619757	26.01919100885331	29.324098827889316	22.04214347563762
30	22.889481297475783	25.372295978774268	29.882637463616096	21.855585260133847
31	24.16269694009184	26.393064780383867	27.8099226755326	21.634315603991688
32	26.230489014775713	25.82177757918626	27.456623321544065	20.491110084493958
33	24.94099055091474	24.86348937072576	27.73330505928714	22.46221501907236
34	23.626121179532618	25.202020584515417	30.480305359698885	20.691552876253084
35	22.939213325154057	25.759170762616662	29.223498761748246	22.078117150481038
36	24.700156264594582	27.395278739179496	27.616169725060146	20.288395271165776
37	23.841205888070117	27.090070508402693	27.653784304370106	21.41493929915708
38	23.156317608419606	27.427465711407823	27.941321256276456	21.47489542389611
39	24.744460685191218	25.44588423292765	27.435417786899524	22.374237294981608
40	24.59248768690533	24.04493048876233	30.152629360072503	21.20995246425984
41	22.73208069210826	25.57538946236396	28.49935499832123	23.19317484720655
42	22.267199834394873	25.304387778543536	29.77888181196245	22.649530575099146
43	23.52842425206312	24.74938339859084	27.969469079048682	23.752723270297356
44	23.00585929117976	24.58933210139275	28.57281702905411	23.831991578373383
45	22.60535237792302	24.760364836174624	27.627529832905434	25.006752952996926
46	23.7934934351199	25.82480694127834	27.85258619166269	22.52911343193907
47	21.62838310322804	25.575010792102454	30.716343156039915	22.080262948629592
48	22.012733418660364	26.389025630927765	28.963604738932098	22.634636211479766
49	22.861964591806082	25.09069152763157	30.244772457039858	21.80257142352249
50	21.911880905678288	25.194447179285223	29.974023220060435	22.919648694976054
51	21.586981821302977	25.079962536888793	28.42475695680382	24.908298685004404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	781.0
2	1260.0
3	5001.0
4	8742.0
5	5766.5
6	2791.0
7	2613.5
8	2436.0
9	2406.5
10	2377.0
11	2347.0
12	2317.0
13	2274.0
14	2231.0
15	2203.0
16	2175.0
17	2226.0
18	2277.0
19	2204.0
20	2131.0
21	2538.0
22	2945.0
23	3333.5
24	3722.0
25	4679.5
26	6862.5
27	8088.0
28	9347.5
29	10607.0
30	12767.5
31	14928.0
32	17601.5
33	20275.0
34	22855.5
35	25436.0
36	27144.5
37	28853.0
38	30128.0
39	31403.0
40	33750.5
41	36098.0
42	38210.5
43	40323.0
44	43376.0
45	46429.0
46	58354.0
47	70279.0
48	64741.0
49	59203.0
50	58074.0
51	56945.0
52	52542.5
53	48140.0
54	45965.0
55	43790.0
56	42892.5
57	41995.0
58	39727.0
59	37459.0
60	35647.0
61	33835.0
62	30899.0
63	27963.0
64	24508.0
65	21053.0
66	18752.5
67	16452.0
68	13961.0
69	11470.0
70	9748.0
71	8026.0
72	6493.5
73	4961.0
74	4096.5
75	2465.5
76	1699.0
77	1303.0
78	907.0
79	665.5
80	424.0
81	342.0
82	260.0
83	231.0
84	202.0
85	133.5
86	65.0
87	47.5
88	30.0
89	37.0
90	44.0
91	30.5
92	17.0
93	14.0
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	792246.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.398040057304684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19116168618517	22.66660986406201
2	8.57760775891563	5.386401440205607
3	3.9615189527230816	3.731517922961131
4	2.5095698829010322	3.1518230283972812
5	1.722407951753107	2.7040116932082086
6	1.2377015599967303	2.3316841895859497
7	0.9329138430934362	2.0504166350823234
8	0.6998578612621862	1.7579332129863765
9	0.6166516653917393	1.7425488322236127
>10	7.436360935379235	48.34039367918281
>50	0.08687600979077456	1.70614308213178
>100	0.023008257554445796	1.4747213857490242
>500	0.0019834704788315328	0.43307485289268205
>1k	0.0019834704788315328	1.8846291628182836
>5k	3.966940957663065E-4	0.6380910185128588
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5123	0.6466425832380347	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCG	4460	0.5629564554443949	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGC	4280	0.5402362397538139	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTC	4013	0.5065345864794522	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1339	0.16901316005382166	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCT	1039	0.1311461339028534	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	877	0.11069793978133054	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05642186896494271	0.0
2	0.0	0.0	0.0	0.358348290808663	0.0
3	0.0	0.0	0.0	0.48861086076799376	0.0
4	0.0	0.0	0.0	0.7644090345675459	0.0
5	0.0	0.0	0.0	1.7095700072957136	0.0
6	0.0	0.0	0.0	2.1256024012743517	0.0
7	0.0	0.0	0.0	2.478649308421879	0.0
8	0.0	0.0	0.0	2.9511035713654596	0.0
9	0.0	0.0	0.0	3.15760508730874	0.0
10	0.0	0.0	0.0	4.166382663970534	0.0
11	0.0	0.0	0.0	4.661935812866206	0.0
12	0.0	0.0	0.0	5.693181158377574	0.0
13	0.0	0.0	0.0	5.9080134200740675	0.0
14	0.0	0.0	0.0	6.037013755828366	0.0
15	0.0	0.0	0.0	6.255632720139957	0.0
16	0.0	0.0	0.0	6.546072810717883	0.0
17	0.0	0.0	0.0	6.850902371233177	0.0
18	0.0	0.0	0.0	7.1826175203156595	0.0
19	0.0	0.0	0.0	7.429384307399469	0.0
20	0.0	0.0	0.0	7.660499390340879	0.0
21	0.0	0.0	0.0	7.941977618063077	0.0
22	0.0	0.0	0.0	8.235699517574087	0.0
23	0.0	0.0	0.0	8.507837212179046	0.0
24	0.0	0.0	0.0	8.733398464618313	0.0
25	0.0	0.0	0.0	8.952396099191413	0.0
26	0.0	0.0	0.0	9.162810541170293	0.0
27	0.0	0.0	0.0	9.405790625639007	0.0
28	0.0	0.0	0.0	9.631351878078274	0.0
29	0.0	0.0	0.0	9.876477760695542	0.0
30	0.0	0.0	0.0	10.232554029935146	0.0
31	0.0	0.0	0.0	10.494846297740853	0.0
32	0.0	0.0	0.0	10.751332288203411	0.0
33	0.0	0.0	0.0	11.017916152306228	0.0
34	0.0	0.0	0.0	11.284878686670554	0.0
35	0.0	0.0	0.0	11.576202341192003	0.0
36	0.0	0.0	0.0	11.858942803119232	0.0
37	0.0	0.0	0.0	12.128303582473121	0.0
38	0.0	0.0	0.0	12.45484357131497	0.0
39	0.0	0.0	0.0	12.949260709426113	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAC	20	7.0319563E-4	45.000004	45
CGGTCTA	40	6.8102963E-9	45.000004	31
ATCGTAC	25	3.8897044E-5	45.0	6
AAACCGT	25	3.8897044E-5	45.0	35
CGAATAT	90	0.0	42.5	14
TACGGGA	105	0.0	40.714287	4
CGTTTTT	2255	0.0	40.609756	1
CGAATGG	50	1.0804797E-9	40.5	2
TACGGCT	575	0.0	39.521744	7
TCACGAC	40	3.4564073E-7	39.375004	25
ACGGGAT	270	0.0	39.166668	5
CGTTTTA	140	0.0	38.571426	1
ACGGTTG	35	6.245831E-6	38.571426	1
GCTACGA	100	0.0	38.25	10
TCGTACT	30	1.1396018E-4	37.499996	7
TCACGTA	30	1.1396018E-4	37.499996	32
TTTAGCG	55	2.746674E-9	36.81818	1
ACGGCTG	710	0.0	36.126762	8
GGGCGAT	785	0.0	36.114647	7
CGATTTC	25	0.002106714	36.0	26
>>END_MODULE