FastQCFastQC Report
Sat 14 Jan 2017
SRR2936613.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936613.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253622
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229861.8335670560982498No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC28060.223831426059849No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.1348891452128313No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG16450.1312197775725059No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC15440.12316312253613929No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT15430.12308335367439308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105900.043.49151
CACGACC2150.038.72093227
CGTTTTA6950.038.201441
TTGCACG659.094947E-1238.076921
CGAATAT2150.037.6744214
CGGTCTA601.5643309E-1037.49999631
GCTACGA2350.037.34042710
GTTTTTT129250.036.557062
CGCACGG1050.036.428572
ACACGAC2300.036.19565226
GTCGATT250.002107181436.031
CGGGTTA702.1827873E-1135.3571436
AACACGT2100.035.3571441
ATGCGCG458.700754E-735.01
GGCGATA2950.034.3220338
CGACGGT1050.034.28571328
GACACGA2450.033.9795925
GCGAGAC2400.033.7521
TTATGCG606.4337655E-933.751
TATTGCG401.5606796E-533.751
Produced by FastQC (version 0.11.5)