##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936612.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	486864
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146024351769693	31.0	31.0	33.0	28.0	34.0
2	31.195114035952546	31.0	31.0	34.0	28.0	34.0
3	30.944160997732425	31.0	31.0	34.0	27.0	34.0
4	34.935452200203756	35.0	35.0	37.0	32.0	37.0
5	35.188317476749155	35.0	35.0	37.0	33.0	37.0
6	35.167605327155016	36.0	35.0	37.0	32.0	37.0
7	31.082357701534722	35.0	35.0	37.0	0.0	37.0
8	33.10783709619113	36.0	35.0	37.0	17.0	37.0
9	36.32673190048966	39.0	35.0	39.0	32.0	39.0
10	36.15583201879786	37.0	35.0	39.0	32.0	39.0
11	36.39109278977291	38.0	35.0	39.0	32.0	39.0
12	36.41319957934865	38.0	35.0	39.0	32.0	39.0
13	36.3167927799139	38.0	35.0	39.0	32.0	39.0
14	37.35924405994282	39.0	36.0	40.0	32.0	41.0
15	37.553922656018926	39.0	36.0	41.0	33.0	41.0
16	37.48011970488679	39.0	36.0	41.0	33.0	41.0
17	37.353246491833445	39.0	36.0	40.0	32.0	41.0
18	37.279568832363864	39.0	36.0	40.0	32.0	41.0
19	37.10357101777909	39.0	35.0	40.0	32.0	41.0
20	37.0688344178251	39.0	35.0	40.0	32.0	41.0
21	37.06815044858523	39.0	35.0	40.0	32.0	41.0
22	37.14721359558316	39.0	35.0	40.0	32.0	41.0
23	37.22266793190706	39.0	35.0	40.0	32.0	41.0
24	37.163801390121264	39.0	35.0	40.0	32.0	41.0
25	36.86638979263203	39.0	35.0	40.0	31.0	41.0
26	36.848851013835485	39.0	35.0	40.0	31.0	41.0
27	36.84868258897762	39.0	35.0	40.0	31.0	41.0
28	36.88462897236189	39.0	35.0	40.0	31.0	41.0
29	36.839425383680044	39.0	35.0	40.0	31.0	41.0
30	36.586455355088894	38.0	35.0	40.0	31.0	41.0
31	36.56267047881955	38.0	35.0	40.0	31.0	41.0
32	36.352576900325346	38.0	35.0	40.0	30.0	41.0
33	36.176862532452596	38.0	35.0	40.0	30.0	41.0
34	36.072769808406456	38.0	35.0	40.0	30.0	41.0
35	35.945327647967396	38.0	35.0	40.0	29.0	41.0
36	35.90461401952085	38.0	35.0	40.0	29.0	41.0
37	35.82951296460613	38.0	35.0	40.0	29.0	41.0
38	35.734969108416315	38.0	35.0	40.0	29.0	41.0
39	35.732298958230636	38.0	35.0	40.0	29.0	41.0
40	35.607855581846266	38.0	35.0	40.0	28.0	41.0
41	35.441741430871865	38.0	34.0	40.0	27.0	41.0
42	35.40364044168392	38.0	34.0	40.0	27.0	41.0
43	35.346688192185084	38.0	34.0	40.0	27.0	41.0
44	35.168603552532126	38.0	34.0	40.0	26.0	41.0
45	35.07466972296165	38.0	34.0	40.0	26.0	41.0
46	34.96537842190016	38.0	34.0	40.0	26.0	41.0
47	34.91331665516448	37.0	34.0	40.0	26.0	41.0
48	34.83643070754872	37.0	34.0	40.0	25.0	41.0
49	34.736612277761346	37.0	34.0	40.0	25.0	41.0
50	34.56006400144599	37.0	34.0	40.0	24.0	41.0
51	32.89715608465608	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	22.0
9	37.0
10	40.0
11	35.0
12	19.0
13	25.0
14	26.0
15	38.0
16	88.0
17	137.0
18	244.0
19	430.0
20	714.0
21	1131.0
22	1589.0
23	2502.0
24	3494.0
25	4833.0
26	6389.0
27	7504.0
28	8271.0
29	9453.0
30	11442.0
31	14239.0
32	18736.0
33	25950.0
34	40574.0
35	43493.0
36	48347.0
37	70936.0
38	92937.0
39	73181.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64250386144796	20.009489302967566	24.290150842945874	13.0578559926386
2	33.85853133523941	23.975483913372113	24.63727036708403	17.528714384304447
3	32.5189375267015	23.689161655000166	27.84740707877354	15.944493739524795
4	29.110593512767423	25.505069177429423	26.381700023004374	19.002637286798777
5	26.18780604029051	28.530143941634623	26.05532551184725	19.22672450622761
6	26.297898386407702	34.42275460909001	25.63672812120017	13.642618883302113
7	67.31140523842387	18.2500657267738	10.64979131749318	3.7887377173091457
8	77.38855203917315	7.161342797988761	10.851901146932203	4.598204015905879
9	71.06173387229288	8.25076407374544	13.47665877945381	7.21084327450787
10	41.32139570804167	25.22306023858819	20.09349633573236	13.362047717637779
11	32.401040126195404	25.163495349830754	25.37217785664991	17.063286667323936
12	30.955667291071016	21.971638897104736	28.94299023957409	18.129703572250154
13	25.120362154523647	24.465764566696244	30.300248118571098	20.11362516020901
14	19.903710276381084	27.487552992211377	31.02303723421736	21.58569949719018
15	19.644500312202176	25.47692990239574	35.93981070689145	18.938759078510632
16	22.97171283972526	24.116796477044925	32.98025732031943	19.93123336291038
17	22.679023300141314	24.489385126031088	30.571576456669625	22.260015117157973
18	23.675400111735513	25.591335568043643	30.62908738374577	20.104176936475074
19	25.13782082881462	26.95372835124388	28.012545597949323	19.895905221992177
20	27.70773768444576	25.45988202044103	28.581903776003152	18.25047651911006
21	25.34876269348319	26.7674340267508	28.962092083210095	18.92171119655592
22	23.32027013704032	25.023415163166717	29.2601219231654	22.39619277662756
23	22.7876778730816	26.137894771435143	30.201657957869138	20.872769397614118
24	24.024162805218705	23.84567353511453	30.55843110190936	21.571732557757404
25	22.099600709849156	25.63015544382004	29.26525682736863	23.004987018962176
26	21.18250681915278	26.796805678793255	29.57992375694239	22.44076374511157
27	21.4445923296855	27.91149068322981	29.280661539978308	21.36325544710638
28	19.79218015708699	27.363288310493278	31.24301653028361	21.60151500213612
29	22.73406947319991	26.868694337638438	29.889250386144795	20.507985803016858
30	22.257755759308555	27.098327253606758	30.499482401656312	20.144434585428375
31	24.920922475270302	26.166444838805088	28.30092181800256	20.61171086792205
32	25.491513030332907	27.9572940287226	27.2108843537415	19.340308587202998
33	24.346018600676985	28.242178513917644	26.8076916757041	20.604111209701273
34	22.819514279141607	27.069777186236816	30.18953958394952	19.921168950672055
35	21.60397975615367	27.62640080186664	29.57602122974794	21.193598212231752
36	23.936664037595715	29.000706562818362	27.572587005816818	19.4900423937691
37	23.13479739721976	29.63455913766473	28.546781031253083	18.683862433862434
38	21.669090341450588	29.06746031746032	27.39040060468632	21.873048736402772
39	24.217645995596307	27.193425679450524	27.6911005948273	20.89782773012587
40	24.16691314206842	25.253869663807553	29.50064083604456	21.078576358079467
41	21.922138420585625	26.29851457491209	28.89102500903743	22.888321995464853
42	22.61863682671136	25.819736107003187	29.613608728515562	21.948018337769888
43	22.788704853922244	25.977069571790068	28.140096618357486	23.0941289559302
44	21.648961516973937	26.233609385783296	29.133187091261625	22.984242005981137
45	22.256523382299783	26.605376450096944	27.892799631930064	23.24530053567321
46	22.1669706529955	28.033496007098492	27.540339807420555	22.259193532485458
47	21.08412205461895	26.381289230668116	30.60875316310099	21.92583555161195
48	21.861751947155675	24.735038943113477	30.614504255808605	22.788704853922244
49	21.97595221663545	24.7789937230931	31.281014821387494	21.96403923888396
50	20.277736698544153	25.08955272930428	30.67817706792862	23.954533504222944
51	19.69297380788064	25.507328535278845	28.985096454040555	25.81460120279996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	231.0
1	648.0
2	1065.0
3	3755.0
4	6445.0
5	4383.0
6	2321.0
7	2121.0
8	1921.0
9	1865.5
10	1810.0
11	1801.5
12	1793.0
13	1768.5
14	1744.0
15	1687.0
16	1630.0
17	1687.5
18	1745.0
19	1759.5
20	1774.0
21	2010.0
22	2246.0
23	2269.0
24	2292.0
25	3139.5
26	4338.5
27	4690.0
28	5706.0
29	6722.0
30	7834.0
31	8946.0
32	9926.5
33	10907.0
34	12271.0
35	13635.0
36	14894.5
37	16154.0
38	17272.5
39	18391.0
40	21261.0
41	24131.0
42	27172.5
43	30214.0
44	37115.0
45	44016.0
46	43246.0
47	42476.0
48	41317.0
49	40158.0
50	35000.0
51	29842.0
52	27664.0
53	25486.0
54	24525.0
55	23564.0
56	22997.0
57	22430.0
58	22209.5
59	21989.0
60	21814.5
61	21640.0
62	20040.5
63	18441.0
64	14906.0
65	11371.0
66	9426.5
67	7482.0
68	6175.5
69	4869.0
70	4096.0
71	3323.0
72	2630.5
73	1938.0
74	1721.0
75	1177.5
76	851.0
77	598.5
78	346.0
79	261.5
80	177.0
81	121.5
82	66.0
83	53.5
84	41.0
85	29.0
86	17.0
87	18.5
88	20.0
89	16.5
90	13.0
91	8.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	486864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.4902140615569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.00240925365061	24.783565269756984
2	9.453987832070839	6.3323215246281315
3	3.729776729313947	3.74733063199613
4	2.1720636566814875	2.909715072703642
5	1.359122434148991	2.275865062775699
6	1.0020968011021298	2.0136261827587045
7	0.7582706961741005	1.7776253552033476
8	0.5888971862358469	1.577783426182964
9	0.4819939418183932	1.4527872259084431
>10	6.2789357132374874	46.24147868440272
>50	0.1314746008749053	2.776828035024561
>100	0.03730209606218244	2.11697554377527
>500	6.115097715111876E-4	0.13700847816442446
>1k	0.0030575488575559375	1.8570895067189852
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3355	0.6891041440730882	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	1667	0.34239541227118864	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	1596	0.32781228433402343	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1260	0.2587991718426501	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	1190	0.24442144007361397	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	669	0.13741003647835948	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	499	0.10249268789641461	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.053961681290874E-4	0.0	0.0	0.12303230470932335	0.0
2	2.053961681290874E-4	0.0	0.0	0.7396316014328437	0.0
3	2.053961681290874E-4	0.0	0.0	0.9499572775970292	0.0
4	2.053961681290874E-4	0.0	0.0	1.2859854086562161	0.0
5	2.053961681290874E-4	0.0	0.0	2.289551086134937	0.0
6	2.053961681290874E-4	0.0	0.0	2.732796016957508	0.0
7	2.053961681290874E-4	0.0	0.0	3.2481350027933877	0.0
8	2.053961681290874E-4	0.0	0.0	4.036650892240955	0.0
9	2.053961681290874E-4	0.0	0.0	4.297298629596766	0.0
10	4.107923362581748E-4	0.0	0.0	5.280735482598836	0.0
11	4.107923362581748E-4	0.0	0.0	6.216931216931217	0.0
12	4.107923362581748E-4	0.0	0.0	7.314773735581189	0.0
13	4.107923362581748E-4	0.0	0.0	7.640532058233922	0.0
14	4.107923362581748E-4	0.0	0.0	7.793757599658221	0.0
15	4.107923362581748E-4	0.0	0.0	8.123418449505406	0.0
16	4.107923362581748E-4	0.0	0.0	8.73036412632686	0.0
17	4.107923362581748E-4	0.0	0.0	9.383729337145486	0.0
18	4.107923362581748E-4	0.0	0.0	10.112885734003747	0.0
19	4.107923362581748E-4	0.0	0.0	10.595566729107102	0.0
20	4.107923362581748E-4	0.0	0.0	11.056270334220645	0.0
21	4.107923362581748E-4	0.0	0.0	11.583522297808011	0.0
22	4.107923362581748E-4	0.0	0.0	12.130286897367643	0.0
23	4.107923362581748E-4	0.0	0.0	12.678489270104176	0.0
24	4.107923362581748E-4	0.0	0.0	13.129333859147524	0.0
25	4.107923362581748E-4	0.0	0.0	13.495966019257946	0.0
26	4.107923362581748E-4	0.0	0.0	13.858695652173912	0.0
27	4.107923362581748E-4	0.0	0.0	14.217933550231686	0.0
28	4.107923362581748E-4	0.0	0.0	14.599765026783661	0.0
29	4.107923362581748E-4	0.0	0.0	15.015281474908804	0.0
30	4.107923362581748E-4	0.0	0.0	15.47968221104867	0.0
31	4.107923362581748E-4	0.0	0.0	15.906084656084657	0.0
32	4.107923362581748E-4	0.0	0.0	16.340292155509548	0.0
33	4.107923362581748E-4	0.0	0.0	16.7473873607414	0.0
34	4.107923362581748E-4	0.0	0.0	17.167011732229124	0.0
35	4.107923362581748E-4	0.0	0.0	17.573490748956587	0.0
36	4.107923362581748E-4	0.0	0.0	18.001330967169476	0.0
37	4.107923362581748E-4	0.0	0.0	18.433073712576817	0.0
38	6.161885043872621E-4	0.0	0.0	18.878988793585066	0.0
39	6.161885043872621E-4	0.0	0.0	19.346675868414998	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	35	1.2099918E-7	45.000004	30
GCGCCTA	25	3.8874816E-5	45.0	41
TCCGTGA	25	3.8874816E-5	45.0	36
CCCTTCG	20	7.0292765E-4	45.0	39
CTCGACG	25	3.8874816E-5	45.0	1
CGATGCA	20	7.0292765E-4	45.0	10
TATACGG	20	7.0292765E-4	45.0	2
TACGTTG	20	7.0292765E-4	45.0	1
ATATTCG	20	7.0292765E-4	45.0	16
TACGAAT	100	0.0	42.75	12
CGAATAT	100	0.0	42.75	14
CGTTTTT	1720	0.0	40.944767	1
GCTACGA	110	0.0	40.909092	10
AGCTACG	110	0.0	40.909092	9
CGTTTTA	105	0.0	40.714287	1
ACCTATG	100	0.0	40.5	34
CGTGCGG	40	3.452751E-7	39.375	2
GTAGCCG	35	6.240871E-6	38.57143	43
CCTATGC	105	0.0	38.57143	35
CTGTCCG	30	1.1389554E-4	37.500004	1
>>END_MODULE