##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936611.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731474
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116503115626802	31.0	31.0	33.0	28.0	34.0
2	31.16980234430752	31.0	31.0	34.0	28.0	34.0
3	30.971513683329825	31.0	31.0	34.0	27.0	34.0
4	34.92785662921717	35.0	35.0	37.0	32.0	37.0
5	35.14799842509782	35.0	35.0	37.0	33.0	37.0
6	35.10563191583023	36.0	35.0	37.0	32.0	37.0
7	31.102655733491552	35.0	35.0	37.0	0.0	37.0
8	33.11215983069801	35.0	35.0	37.0	17.0	37.0
9	36.36303682700957	39.0	35.0	39.0	32.0	39.0
10	36.06618553769512	37.0	35.0	39.0	32.0	39.0
11	36.27688885729363	37.0	35.0	39.0	32.0	39.0
12	36.30562672084039	38.0	35.0	39.0	32.0	39.0
13	36.20288622698825	38.0	35.0	39.0	32.0	39.0
14	37.087526009126776	39.0	36.0	40.0	31.0	41.0
15	37.33460792864818	39.0	36.0	41.0	32.0	41.0
16	37.294652988349554	39.0	36.0	40.0	32.0	41.0
17	37.24682217002928	39.0	36.0	40.0	32.0	41.0
18	37.18895271738982	39.0	36.0	40.0	32.0	41.0
19	37.01787213216054	39.0	35.0	40.0	32.0	41.0
20	36.97581868938609	39.0	35.0	40.0	31.0	41.0
21	36.972767316404955	39.0	35.0	40.0	31.0	41.0
22	37.092614364967176	39.0	35.0	40.0	32.0	41.0
23	37.15418456431807	39.0	35.0	40.0	32.0	41.0
24	37.09759335260036	39.0	35.0	40.0	32.0	41.0
25	36.76264774961243	39.0	35.0	40.0	31.0	41.0
26	36.75293448570967	39.0	35.0	40.0	31.0	41.0
27	36.77968321498782	39.0	35.0	40.0	31.0	41.0
28	36.77030762542483	39.0	35.0	40.0	31.0	41.0
29	36.74069071491263	39.0	35.0	40.0	31.0	41.0
30	36.484111533697714	38.0	35.0	40.0	30.0	41.0
31	36.43082050763253	38.0	35.0	40.0	30.0	41.0
32	36.32023284491315	38.0	35.0	40.0	30.0	41.0
33	36.2057407371964	38.0	35.0	40.0	30.0	41.0
34	36.13401706690873	38.0	35.0	40.0	30.0	41.0
35	36.027992792635146	38.0	35.0	40.0	29.0	41.0
36	35.94552779729697	38.0	35.0	40.0	29.0	41.0
37	35.84399582213449	38.0	35.0	40.0	29.0	41.0
38	35.66400172801767	38.0	35.0	40.0	28.0	41.0
39	35.58911731654167	38.0	34.0	40.0	27.0	41.0
40	35.47533200086401	38.0	34.0	40.0	27.0	41.0
41	35.28043512141238	38.0	34.0	40.0	26.0	41.0
42	35.31468377549988	38.0	34.0	40.0	27.0	41.0
43	35.29131725802968	38.0	34.0	40.0	27.0	41.0
44	35.08529489769971	38.0	34.0	40.0	26.0	41.0
45	34.9920776404903	38.0	34.0	40.0	26.0	41.0
46	34.939507077490106	37.0	34.0	40.0	26.0	41.0
47	34.875910832100665	37.0	34.0	40.0	26.0	41.0
48	34.77254557236484	37.0	34.0	40.0	25.0	41.0
49	34.70328678804715	37.0	34.0	40.0	25.0	41.0
50	34.52170548782322	37.0	33.0	40.0	24.0	41.0
51	32.834911425423186	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	30.0
9	75.0
10	64.0
11	61.0
12	39.0
13	25.0
14	42.0
15	76.0
16	129.0
17	277.0
18	461.0
19	762.0
20	1276.0
21	1976.0
22	2761.0
23	3868.0
24	5523.0
25	7491.0
26	9315.0
27	10802.0
28	12627.0
29	14530.0
30	18244.0
31	22775.0
32	29144.0
33	40335.0
34	58939.0
35	63016.0
36	74305.0
37	107774.0
38	137843.0
39	106883.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78665543819739	20.52636183924514	24.7850231177048	10.901959604852667
2	34.279960736813614	23.254141637296748	25.749377284770205	16.71652034111944
3	34.40669114691705	22.604631196734264	27.04853487615418	15.94014278019451
4	29.885682881414787	25.36522145694857	25.775762364759373	18.973333296877264
5	26.571005941427856	29.449850575686902	24.947571615669183	19.03157186721606
6	27.217918887069125	34.902402546091864	24.564509469919642	13.315169096919371
7	70.31186344285648	17.645329840841917	8.61014882278796	3.432657893513645
8	81.80673544104097	5.807178382280163	8.408501190746357	3.977584985932514
9	75.576848937898	7.131900792099241	10.835244998455174	6.456005271547588
10	44.963730768284314	23.465358987469138	17.49987012525394	14.07104011899261
11	32.93213429322163	25.78792957781138	23.520863352627707	17.759072776339284
12	29.653412151354665	23.079972767316406	28.635467562756844	18.63114751857209
13	26.761443332230538	23.00669606848637	31.055922698551147	19.175937900731945
14	22.433469952452175	25.631259620984476	32.150698452713286	19.784571973850063
15	21.249422399155677	25.101644077574868	34.25261868501136	19.396314838258093
16	26.83663397468673	23.810825811990583	29.32435055791457	20.02818965540812
17	26.18958431878645	24.052666260181496	27.489015330688442	22.26873409034361
18	26.32793510090584	24.234080773889435	29.930797266888504	19.50718685831622
19	25.943642562825197	25.899075018387528	27.418336126779625	20.738946292007647
20	25.732561922911817	26.985374736490975	27.724977237741875	19.55708610285533
21	26.199290747176253	25.681705706559633	29.752937219914855	18.366066326349262
22	24.834648941725884	23.032260886921478	30.080905131282858	22.052185040069777
23	23.669604114431955	24.2883547467169	30.34270527728942	21.69933586156172
24	24.353018699229228	25.327626135720475	29.536388169641025	20.782966995409268
25	24.48152634269981	24.815099374687275	28.418234961187956	22.285139321424957
26	22.64523414366061	25.990260761148036	27.75751427938655	23.6069908158048
27	22.3160358399615	26.33094272660409	29.671184485026124	21.681836948408282
28	21.384902265835834	26.300593049103593	29.376027035820822	22.938477649239754
29	23.698176558565308	26.08705162452801	28.568069405064296	21.64670241184239
30	24.641340635483967	24.662530725630713	28.880725767423037	21.81540287146228
31	25.274582555224107	25.52394206766064	27.543426013774923	21.65804936334032
32	26.184115908425998	25.792030885581717	26.99972931368716	21.024123892305127
33	25.454766676600943	24.799787825678017	27.49380018975384	22.2516453079672
34	23.658940714229075	26.059846282984765	29.109715451266894	21.171497551519263
35	23.7843040217424	25.774395262169264	28.53826656859984	21.903034147488494
36	25.690591873395363	25.6812955757826	27.595922753235246	21.03218979758679
37	22.956249982911217	27.283403101135516	27.71773159401428	22.04261532193899
38	23.133016347812774	26.924401960971956	27.783352518339683	22.15922917287559
39	23.31566125385181	24.88318108367488	28.47114183142531	23.330015831047994
40	25.210875574524866	24.192384144891	28.526646196583883	22.070094084000253
41	22.075699204619713	24.97969852653683	28.3932169837889	24.55138528505456
42	22.19586752229061	24.891110278697536	30.068054366935804	22.844967832076055
43	22.619122484189457	24.590074288354746	29.535157777309923	23.25564545014587
44	22.750501043099273	24.976007349543526	28.866371190226857	23.407120417130344
45	22.245356636052684	25.150449640041888	27.6573056595313	24.946888064374125
46	23.836937471461734	25.274582555224107	27.940022475166582	22.948457498147576
47	21.608833670096274	25.27800031169939	30.297454181556695	22.815711836647644
48	21.617583126672994	26.37250264534351	28.740324331418478	23.26958989656502
49	23.374856796003684	24.514063384344485	29.45518227578834	22.65589754386349
50	22.157315229249434	25.171366309670614	28.89220942917998	23.779109031899974
51	21.889636542105393	25.29659290692492	28.123350932500678	24.690419618469008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	674.5
2	1106.0
3	2903.5
4	4701.0
5	3267.5
6	1834.0
7	1778.0
8	1722.0
9	1685.0
10	1648.0
11	1706.5
12	1765.0
13	1791.0
14	1817.0
15	1812.5
16	1808.0
17	1875.5
18	1943.0
19	1852.0
20	1761.0
21	2007.0
22	2253.0
23	2711.0
24	3169.0
25	4188.0
26	5647.5
27	6088.0
28	7283.0
29	8478.0
30	10409.5
31	12341.0
32	14188.5
33	16036.0
34	17752.5
35	19469.0
36	21502.5
37	23536.0
38	26379.0
39	29222.0
40	31621.0
41	34020.0
42	37654.0
43	41288.0
44	49321.0
45	57354.0
46	57920.0
47	58486.0
48	58562.5
49	58639.0
50	54096.0
51	49553.0
52	46371.5
53	43190.0
54	42615.0
55	42040.0
56	41082.5
57	40125.0
58	39662.5
59	39200.0
60	35747.5
61	32295.0
62	28882.5
63	25470.0
64	22595.5
65	19721.0
66	17401.5
67	15082.0
68	12902.0
69	10722.0
70	8767.0
71	6812.0
72	5798.5
73	4785.0
74	3939.5
75	2410.0
76	1726.0
77	1329.0
78	932.0
79	638.5
80	345.0
81	293.0
82	241.0
83	163.0
84	85.0
85	64.0
86	43.0
87	41.5
88	40.0
89	30.5
90	21.0
91	17.0
92	13.0
93	8.5
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.53182635178719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7286168674435	23.247979442216767
2	8.85614715276057	5.585009883334417
3	3.8479441773242447	3.6399812283227613
4	2.2470045801190066	2.834086329279321
5	1.556707264510382	2.4542911572553514
6	1.082635557847544	2.0482485847391407
7	0.8413734446314554	1.857102894718686
8	0.6180154843467695	1.5589725548110391
9	0.48369736758128723	1.3726675261250754
>10	6.44804472357111	47.3833413244412
>50	0.2569880194349498	4.837875341449801
>100	0.029801971968561575	1.7183085078203153
>500	4.319126372255301E-4	0.12202618871419532
>1k	0.0025914758233531805	1.3401090367719664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2525	0.34519340400342324	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGC	1646	0.22502508633252855	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	1520	0.20779959369711024	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1498	0.20479196799886257	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1382	0.18893357795355678	Illumina PCR Primer Index 5 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	1269	0.17348531868528477	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	896	0.12249239207408602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07286656805299983	0.0
2	0.0	0.0	0.0	0.44089058531130293	0.0
3	0.0	0.0	0.0	0.5672108646377041	0.0
4	0.0	0.0	0.0	0.8023525101370657	0.0
5	0.0	0.0	0.0	1.5445525063091785	0.0
6	0.0	0.0	0.0	1.926110839209596	0.0
7	0.0	0.0	0.0	2.3131375824704636	0.0
8	0.0	0.0	0.0	2.954308697233258	0.0
9	0.0	0.0	0.0	3.1940984915390023	0.0
10	0.0	0.0	0.0	3.9447745237698126	0.0
11	0.0	0.0	0.0	4.732909166969708	0.0
12	0.0	0.0	0.0	5.546335208086686	0.0
13	0.0	0.0	0.0	5.808272064352253	0.0
14	1.3671025901125673E-4	0.0	0.0	5.93090116668535	0.0
15	2.7342051802251347E-4	0.0	0.0	6.173151745653297	0.0
16	2.7342051802251347E-4	0.0	0.0	6.604746033351835	0.0
17	2.7342051802251347E-4	0.0	0.0	7.072158408911322	0.0
18	2.7342051802251347E-4	0.0	0.0	7.6287058733461475	0.0
19	2.7342051802251347E-4	0.0	0.0	7.9588611488583325	0.0
20	2.7342051802251347E-4	0.0	0.0	8.302140609235598	0.0
21	2.7342051802251347E-4	0.0	0.0	8.728403196832696	0.0
22	2.7342051802251347E-4	0.0	0.0	9.14974421510539	0.0
23	2.7342051802251347E-4	0.0	0.0	9.579287848918758	0.0
24	2.7342051802251347E-4	0.0	0.0	9.920790075928878	0.0
25	2.7342051802251347E-4	0.0	0.0	10.243152866677422	0.0
26	2.7342051802251347E-4	0.0	0.0	10.55416870592803	0.0
27	2.7342051802251347E-4	0.0	0.0	10.897174745787273	0.0
28	4.1013077703377015E-4	0.0	0.0	11.22104134938494	0.0
29	4.1013077703377015E-4	0.0	0.0	11.593166674413581	0.0
30	4.1013077703377015E-4	0.0	0.0	12.102412389230512	0.0
31	5.468410360450269E-4	0.0	0.0	12.516507763775609	0.0
32	5.468410360450269E-4	0.0	0.0	12.868810101247618	0.0
33	5.468410360450269E-4	0.0	0.0	13.205664179451354	0.0
34	5.468410360450269E-4	0.0	0.0	13.60540497680027	0.0
35	5.468410360450269E-4	0.0	0.0	14.027156125849997	0.0
36	5.468410360450269E-4	0.0	0.0	14.416917074291089	0.0
37	5.468410360450269E-4	0.0	0.0	14.785487932585436	0.0
38	5.468410360450269E-4	0.0	0.0	15.230616535926089	0.0
39	5.468410360450269E-4	0.0	0.0	15.929342669732621	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGT	20	7.0316024E-4	45.0	42
CGTTAGG	40	6.8084773E-9	45.0	2
AAACGCG	25	3.8894097E-5	45.0	1
GTATGCG	40	6.8084773E-9	45.0	1
TTCGGAC	25	3.8894097E-5	45.0	14
ATAACGC	35	1.2111195E-7	45.0	11
CCCGCGA	25	3.8894097E-5	45.0	29
ATCGATC	20	7.0316024E-4	45.0	12
AACCCGC	90	0.0	40.0	21
CGGCTAG	40	3.4559162E-7	39.375	2
CGTTTTT	1100	0.0	39.068184	1
CCATACG	75	0.0	39.0	2
CTATGCG	35	6.2451745E-6	38.571426	1
CTCGTAC	35	6.2451745E-6	38.571426	29
CGACAAT	70	0.0	38.571426	20
TCGACAA	70	0.0	38.571426	19
CTACGGG	100	0.0	38.25	3
ACGGGAC	145	0.0	37.24138	5
ACGGGAT	135	0.0	36.666664	5
TTACACG	80	0.0	36.5625	34
>>END_MODULE