##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936609.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	238179
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.964745002708046	31.0	30.0	33.0	28.0	34.0
2	31.01157112927672	31.0	31.0	33.0	28.0	34.0
3	30.67062167529463	31.0	30.0	33.0	27.0	34.0
4	34.67098274826916	35.0	35.0	37.0	32.0	37.0
5	34.91291003824855	35.0	35.0	37.0	32.0	37.0
6	34.88952006684048	35.0	35.0	37.0	32.0	37.0
7	30.676487011869224	35.0	35.0	37.0	0.0	37.0
8	32.64701338069267	35.0	33.0	37.0	17.0	37.0
9	35.65390315686941	37.0	34.0	39.0	31.0	39.0
10	35.71678023671272	37.0	34.0	39.0	31.0	39.0
11	35.95773766788844	37.0	35.0	39.0	31.0	39.0
12	36.07167718396668	37.0	35.0	39.0	32.0	39.0
13	35.77621032920619	37.0	35.0	39.0	30.0	39.0
14	36.97509436180352	39.0	36.0	40.0	31.0	41.0
15	36.99993282363265	39.0	35.0	40.0	31.0	41.0
16	37.28908929838483	39.0	36.0	40.0	32.0	41.0
17	36.88232379848769	38.0	35.0	40.0	31.0	41.0
18	36.77693247515524	38.0	35.0	40.0	31.0	41.0
19	36.48296869161429	38.0	35.0	40.0	30.0	41.0
20	36.1872415284303	38.0	34.0	40.0	30.0	41.0
21	36.5298116122748	38.0	35.0	40.0	31.0	41.0
22	36.552920282644564	38.0	35.0	40.0	31.0	41.0
23	36.638112512018274	38.0	35.0	40.0	31.0	41.0
24	36.44781445887337	38.0	35.0	40.0	31.0	41.0
25	36.40837353419067	38.0	35.0	40.0	31.0	41.0
26	36.1827701014783	38.0	35.0	40.0	30.0	41.0
27	36.198527158145765	38.0	35.0	40.0	30.0	41.0
28	36.06968288556086	38.0	35.0	40.0	30.0	41.0
29	35.99033080162399	38.0	35.0	40.0	30.0	41.0
30	35.84817301273412	38.0	35.0	40.0	30.0	41.0
31	35.753970753089064	38.0	35.0	40.0	29.0	41.0
32	35.35563588729485	38.0	34.0	40.0	27.0	41.0
33	35.02726100957683	38.0	34.0	40.0	25.0	41.0
34	34.851531831101816	38.0	34.0	40.0	23.0	41.0
35	34.49265048555918	38.0	34.0	40.0	21.0	41.0
36	34.24676398842887	38.0	33.0	40.0	21.0	41.0
37	34.300362332531414	38.0	34.0	40.0	20.0	41.0
38	33.93444846103141	38.0	33.0	40.0	18.0	41.0
39	34.1012893664009	38.0	33.0	40.0	18.0	41.0
40	33.747693961264424	37.0	33.0	40.0	18.0	41.0
41	33.606841913015	37.0	33.0	40.0	17.0	41.0
42	33.58899399191365	37.0	33.0	40.0	17.0	41.0
43	33.541802593847486	37.0	33.0	40.0	16.0	41.0
44	33.53419067172169	37.0	33.0	40.0	16.0	41.0
45	33.45080800574358	37.0	32.0	40.0	16.0	41.0
46	33.29795657887555	37.0	32.0	40.0	15.0	41.0
47	33.067705381246874	37.0	31.0	40.0	15.0	41.0
48	33.0278362072223	37.0	31.0	40.0	15.0	41.0
49	32.980464272668875	37.0	31.0	40.0	14.0	41.0
50	32.86381670928167	36.0	31.0	40.0	13.0	41.0
51	31.03762296424118	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	15.0
10	14.0
11	7.0
12	5.0
13	8.0
14	4.0
15	18.0
16	37.0
17	75.0
18	198.0
19	481.0
20	880.0
21	1231.0
22	1782.0
23	2432.0
24	3388.0
25	4531.0
26	5542.0
27	5670.0
28	5668.0
29	6015.0
30	6753.0
31	8483.0
32	10660.0
33	13827.0
34	19451.0
35	21166.0
36	22359.0
37	30938.0
38	38463.0
39	28070.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.55169851246332	19.420267949735283	21.25544233538641	15.77259120241499
2	32.12121975489023	35.16724816209658	19.310686500489126	13.400845582524068
3	24.061735081598293	34.29689435256677	28.52140616930964	13.119964396525303
4	23.483598470058233	19.18934918695603	42.59023675470969	14.736815588276045
5	32.247595295974875	23.32825312055219	28.142279546055697	16.281872037417237
6	20.454784006986344	38.11797009811948	29.77634468194089	11.650901212953283
7	55.30336427644754	16.828099874464165	24.178454019875808	3.6900818292124833
8	64.57874119884625	14.332497827264367	16.484240844071056	4.60452012981833
9	60.80091023977765	5.207008174524202	16.17522955424282	17.816852031455337
10	39.79569987278476	17.093446525512324	26.747950071164965	16.362903530537956
11	30.676088152188058	20.735245340689147	30.925900268285616	17.662766238837175
12	25.315833889637624	17.971357676369454	34.906100034008034	21.806708399984885
13	20.187338094458372	23.332871495807776	41.97263402734917	14.507156382384679
14	19.085225817557383	29.483287779359223	31.374722372669297	20.0567640304141
15	15.300677221753386	22.394081762036116	46.32524277959014	15.979998236620357
16	17.106461946687155	23.425239000919476	33.88585895481969	25.582440097573674
17	18.345026219775885	24.100361492826824	36.354590455077904	21.20002183231939
18	18.59147951750574	23.77245684968028	36.09764084994899	21.538422782864988
19	18.198497768485048	27.913460044756256	31.648885921932663	22.239156264826036
20	19.995045742907642	25.284344967440454	39.138211177307824	15.582398112344078
21	18.262735169767275	31.68919174234504	33.34215023154854	16.70592285633914
22	17.32268587910773	21.432620004282494	35.71221644225561	25.532477674354165
23	17.3541748013049	30.0689817322266	34.075212340298684	18.501631126169812
24	20.175162377875463	23.12588431389837	32.14599104035201	24.55296226787416
25	14.932046905898504	35.17312609424005	31.52964786987938	18.365179129982074
26	16.732793403280727	24.66044445564051	34.99720798223185	23.60955415884692
27	21.260480562937957	28.68220959866319	29.53241049798681	20.52489934041204
28	14.38623892114754	24.49544250332733	40.32975199324878	20.788566582276356
29	20.768413672070167	20.903186259074058	32.818174566187615	25.51022550266816
30	20.79108569605213	27.794221992702965	33.905592012729926	17.509100298514984
31	20.568983831488083	22.260988584216072	32.01667653319562	25.153351051100227
32	23.49199551597748	26.873905759953647	28.49789444073575	21.136204283333125
33	22.74339887227673	22.75221577049194	28.892135746644332	25.612249610586996
34	20.293140873040862	22.969699259800404	29.010953946401656	27.72620592075708
35	21.91377073545527	26.606879699721635	27.92059753378761	23.558752031035482
36	20.011419982450175	23.397528749385966	34.27548188547269	22.31556938269117
37	20.546311807506118	24.53238950537201	32.127517539329666	22.793781147792206
38	21.343611317538493	25.043349749558107	30.61353016008968	22.999508772813723
39	20.07859634980414	23.69478417492726	29.24523152754861	26.98138794771999
40	22.368470771982416	27.417194630928837	29.73016092938504	20.4841736677037
41	16.667716297406574	25.870038920307834	31.23827037648155	26.22397440580404
42	18.386171744780185	26.24286775912234	34.45559852044051	20.915361975656964
43	21.22563282237309	23.5696681907305	31.577091179323112	23.627607807573295
44	20.800742298859262	23.618790909358086	31.79751363470331	23.78295315707934
45	17.897883524576056	23.811922965500738	30.909106176447125	27.381087333476085
46	25.118083458239393	28.371938751947063	26.75928608315595	19.750691706657598
47	16.510271686420715	23.87867948055874	39.313289584724096	20.297759248296448
48	21.082883041745916	25.573623199358465	28.74854626142523	24.59494749747039
49	17.559482574030454	21.862968607643833	37.01207915055483	23.565469667770877
50	20.581999252662914	22.058619777562253	31.81850624950143	25.54087472027341
51	19.459734065555736	23.473941867251103	28.0431944042086	29.023129662984566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	163.5
2	283.0
3	2708.5
4	5134.0
5	3733.5
6	2333.0
7	2202.5
8	2072.0
9	2023.0
10	1974.0
11	1931.0
12	1888.0
13	1807.5
14	1727.0
15	1652.5
16	1578.0
17	1493.0
18	1408.0
19	1301.0
20	1194.0
21	1217.5
22	1241.0
23	1203.0
24	1165.0
25	1491.5
26	2033.0
27	2248.0
28	2643.5
29	3039.0
30	3439.5
31	3840.0
32	4352.5
33	4865.0
34	5499.0
35	6133.0
36	6829.0
37	7525.0
38	8237.5
39	8950.0
40	9712.0
41	10474.0
42	11973.5
43	13473.0
44	14422.0
45	15371.0
46	16476.5
47	17582.0
48	31819.5
49	46057.0
50	32817.0
51	19577.0
52	18508.0
53	17439.0
54	14555.5
55	11672.0
56	10033.5
57	8395.0
58	7199.5
59	6004.0
60	5207.5
61	4411.0
62	3620.0
63	2829.0
64	2255.5
65	1682.0
66	1329.5
67	977.0
68	809.0
69	641.0
70	592.5
71	544.0
72	400.5
73	257.0
74	210.0
75	114.5
76	66.0
77	49.0
78	32.0
79	32.5
80	33.0
81	21.5
82	10.0
83	9.0
84	8.0
85	10.5
86	13.0
87	10.0
88	7.0
89	3.5
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	238179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.18216788983298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72899994462949	33.20959893463012
2	8.39299999409716	7.080698697007473
3	3.4769999975546084	4.400021929493921
4	2.009000068278205	3.389759126831887
5	1.3379999996919336	2.821987031180081
6	0.9849999993135969	2.492966120551891
7	0.6479999995443153	1.9133831341373
8	0.578999999592787	1.9538780152828936
9	0.4659999996722598	1.769120120055364
>10	3.2979999976804995	24.731826838093955
>50	0.04999999996483475	1.3873715009208614
>100	0.01599999998874712	1.3177709239515873
>500	0.0029999999978900845	0.9204149027088231
>1k	0.005999999995780169	3.803144254272571
>5k	0.0029999999978900845	8.808058470881273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	8387	3.521301206235646	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	6823	2.8646522153506395	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	5671	2.3809823704020925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2658	1.1159674026677415	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1621	0.6805805717548566	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	1465	0.6150836135847408	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC	1178	0.4945860046435664	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTA	1076	0.4517610704554138	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	1018	0.4274096372896015	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	804	0.33756124595367354	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	743	0.3119502558999744	No Hit
GAATCTATCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	635	0.2666062079360481	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGTCGCATT	370	0.15534534950604378	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	357	0.14988726965853413	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGT	241	0.10118440332690959	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1985229596228047E-4	0.0	0.0	0.10832189235826836	0.0
2	4.1985229596228047E-4	0.0	0.0	1.1252041531789116	0.0
3	4.1985229596228047E-4	0.0	0.0	1.3007024128911449	0.0
4	4.1985229596228047E-4	0.0	0.0	1.9884204736773603	0.0
5	4.1985229596228047E-4	0.0	0.0	5.201130242380731	0.0
6	4.1985229596228047E-4	0.0	0.0	5.713350043454713	0.0
7	4.1985229596228047E-4	0.0	0.0	6.747866100705772	0.0
8	4.1985229596228047E-4	0.0	0.0	7.87432981077257	0.0
9	4.1985229596228047E-4	0.0	0.0	8.097271379928541	0.0
10	4.1985229596228047E-4	0.0	0.0	12.815151629656686	0.0
11	4.1985229596228047E-4	0.0	0.0	13.663253267500494	0.0
12	4.1985229596228047E-4	0.0	0.0	17.807615280944162	0.0
13	4.1985229596228047E-4	0.0	0.0	18.181703676646556	0.0
14	4.1985229596228047E-4	0.0	0.0	18.39246952921962	0.0
15	4.1985229596228047E-4	0.0	0.0	19.103279466283762	0.0
16	4.1985229596228047E-4	0.0	0.0	19.654545530882235	0.0
17	4.1985229596228047E-4	0.0	0.0	20.251155643444637	0.0
18	4.1985229596228047E-4	0.0	0.0	20.865819404733415	0.0
19	4.1985229596228047E-4	0.0	0.0	21.47250597239891	0.0
20	4.1985229596228047E-4	0.0	0.0	21.865907573715567	0.0
21	4.1985229596228047E-4	0.0	0.0	22.31724879187502	0.0
22	4.1985229596228047E-4	0.0	0.0	22.80931568274281	0.0
23	4.1985229596228047E-4	0.0	0.0	23.342108246318944	0.0
24	4.1985229596228047E-4	0.0	0.0	23.7388686660033	0.0
25	4.1985229596228047E-4	0.0	0.0	24.099101935938936	0.0
26	4.1985229596228047E-4	0.0	0.0	24.447999193883593	0.0
27	4.1985229596228047E-4	0.0	0.0	24.83048463550523	0.0
28	4.1985229596228047E-4	0.0	0.0	25.20415317891166	0.0
29	4.1985229596228047E-4	0.0	0.0	25.600913598596016	0.0
30	4.1985229596228047E-4	0.0	0.0	26.01782692848656	0.0
31	4.1985229596228047E-4	0.0	0.0	26.416686609650725	0.0
32	4.1985229596228047E-4	0.0	0.0	26.797492642088514	0.0
33	4.1985229596228047E-4	0.0	0.0	27.172000890086867	0.0
34	4.1985229596228047E-4	0.0	0.0	27.52803563706288	0.0
35	4.1985229596228047E-4	0.0	0.0	27.92353649985935	0.0
36	4.1985229596228047E-4	0.0	0.0	28.31609839658408	0.0
37	4.1985229596228047E-4	0.0	0.0	28.720416157595757	0.0
38	4.1985229596228047E-4	0.0	0.0	29.148665499477286	0.0
39	4.1985229596228047E-4	0.0	0.0	29.576494989062848	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACCT	45	3.8198777E-10	45.000004	11
CTCGTAC	45	3.8198777E-10	45.000004	29
TACGCTG	30	2.158231E-6	45.000004	1
TCTCGTA	45	3.8198777E-10	45.000004	28
GTACGGT	45	3.8198777E-10	45.000004	32
CGGGTCA	20	7.0220145E-4	45.0	6
CGGCGAA	25	3.8814644E-5	45.0	31
TCCATAG	20	7.0220145E-4	45.0	13
ACCGCGG	20	7.0220145E-4	45.0	2
ACCGAGG	55	1.8189894E-12	45.0	17
CACGCGG	25	3.8814644E-5	45.0	2
GTGTGAT	20	7.0220145E-4	45.0	9
ATATCGG	20	7.0220145E-4	45.0	2
CGGGAAC	25	3.8814644E-5	45.0	6
GGACCGT	20	7.0220145E-4	45.0	8
ACCTTCG	20	7.0220145E-4	45.0	21
CACCCGT	20	7.0220145E-4	45.0	16
TGCAACG	20	7.0220145E-4	45.0	1
CGAAGCG	20	7.0220145E-4	45.0	3
CGACCAC	510	0.0	44.11765	12
>>END_MODULE