##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936608.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443326
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.122422325782832	31.0	31.0	33.0	28.0	34.0
2	31.161880873217452	31.0	31.0	34.0	28.0	34.0
3	30.904072849325328	31.0	31.0	34.0	27.0	34.0
4	34.87627163757596	35.0	35.0	37.0	32.0	37.0
5	35.14929419885141	35.0	35.0	37.0	33.0	37.0
6	35.1476610891308	36.0	35.0	37.0	32.0	37.0
7	31.140343674857778	35.0	35.0	37.0	0.0	37.0
8	33.14826109905577	36.0	35.0	37.0	17.0	37.0
9	36.43228684985767	39.0	35.0	39.0	32.0	39.0
10	36.16319142121148	37.0	35.0	39.0	32.0	39.0
11	36.39306063709324	38.0	35.0	39.0	32.0	39.0
12	36.43814484149362	38.0	35.0	39.0	32.0	39.0
13	36.358625481023	38.0	35.0	39.0	32.0	39.0
14	37.39561406278901	39.0	36.0	40.0	32.0	41.0
15	37.63956772217285	39.0	36.0	41.0	33.0	41.0
16	37.57810505136175	39.0	36.0	41.0	33.0	41.0
17	37.47333790483752	39.0	36.0	41.0	32.0	41.0
18	37.39336740908496	39.0	36.0	40.0	32.0	41.0
19	37.20068076314044	39.0	36.0	40.0	32.0	41.0
20	37.15102430265764	39.0	35.0	40.0	32.0	41.0
21	37.14382643923433	39.0	35.0	40.0	32.0	41.0
22	37.223210459120374	39.0	35.0	40.0	32.0	41.0
23	37.301974167993755	39.0	35.0	40.0	32.0	41.0
24	37.21515995001421	39.0	35.0	40.0	32.0	41.0
25	36.915685071482386	39.0	35.0	40.0	31.0	41.0
26	36.91033686271502	39.0	35.0	40.0	32.0	41.0
27	36.94167723075118	39.0	35.0	40.0	32.0	41.0
28	36.9914757988478	39.0	35.0	40.0	32.0	41.0
29	37.00441661441016	39.0	35.0	40.0	32.0	41.0
30	36.766803661413945	39.0	35.0	40.0	31.0	41.0
31	36.73309483314762	39.0	35.0	40.0	31.0	41.0
32	36.5319087984914	39.0	35.0	40.0	31.0	41.0
33	36.389780883593566	39.0	35.0	40.0	30.0	41.0
34	36.26918114434976	39.0	35.0	40.0	30.0	41.0
35	36.182754902712674	39.0	35.0	40.0	30.0	41.0
36	36.11316728547389	39.0	35.0	40.0	30.0	41.0
37	36.02525455308283	39.0	35.0	40.0	30.0	41.0
38	35.90257282451289	38.0	35.0	40.0	29.0	41.0
39	35.89311477332708	38.0	35.0	40.0	29.0	41.0
40	35.77711210260621	38.0	35.0	40.0	29.0	41.0
41	35.645039993142746	38.0	35.0	40.0	28.0	41.0
42	35.673328430996605	38.0	35.0	40.0	28.0	41.0
43	35.61573875658094	38.0	35.0	40.0	28.0	41.0
44	35.43168007290346	38.0	35.0	40.0	27.0	41.0
45	35.334153647654325	38.0	34.0	40.0	27.0	41.0
46	35.2517943905839	38.0	34.0	40.0	26.0	41.0
47	35.18342934995917	38.0	34.0	40.0	26.0	41.0
48	35.12337873258054	38.0	34.0	40.0	26.0	41.0
49	35.03795626694577	38.0	34.0	40.0	26.0	41.0
50	34.89723589412757	38.0	34.0	40.0	26.0	41.0
51	33.28836341653772	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	41.0
10	35.0
11	28.0
12	12.0
13	20.0
14	19.0
15	31.0
16	64.0
17	105.0
18	237.0
19	364.0
20	630.0
21	944.0
22	1494.0
23	2065.0
24	3131.0
25	4288.0
26	5753.0
27	6474.0
28	7202.0
29	8088.0
30	9862.0
31	12356.0
32	15794.0
33	22526.0
34	34583.0
35	39067.0
36	43403.0
37	65282.0
38	88616.0
39	70788.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12024559804748	20.780418924222808	24.182204517668712	12.917130960060993
2	34.30365013556616	24.348222301421526	24.750860540550295	16.597267022462027
3	32.40843081614884	23.735580588551088	28.82709338049201	15.028895214808063
4	28.750174814921753	25.0759035111859	27.80301629049503	18.37090538339732
5	26.42660254530526	29.366425610047685	25.778772280443736	18.428199564203318
6	25.64410839878554	34.245679251837245	26.486603537802882	13.623608811574329
7	68.51932889115459	17.57081696088206	10.403630736749028	3.5062234112143207
8	79.7886882339407	6.227245864217303	10.217763000590988	3.766302901250998
9	73.6137740624281	8.215399051713637	11.955761674253258	6.215065211605004
10	39.93765310403631	27.408047351159194	19.191294893599746	13.463004651204757
11	30.39343507937725	25.52049733153481	27.145486617071864	16.94058097201608
12	27.067891348578698	24.50544294717657	29.880945399096824	18.545720305147906
13	23.25985843374853	26.811872076079453	31.711877940838118	18.2163915493339
14	19.543857116433504	29.365072204201876	30.97201607846145	20.119054600903173
15	19.25919075353126	27.47616877873168	35.27381655937166	17.990823908365403
16	22.047657931183824	26.535551715893046	32.09534293048456	19.321447422438567
17	22.4881915339953	24.94687882055192	30.384863509020448	22.180066136432334
18	23.679414245949935	25.153949914961	31.737592651908532	19.42904318718054
19	24.296116176357803	27.205938744851423	29.317026296675586	19.180918782115192
20	25.273275197033335	27.153381484505758	29.926058927290526	17.64728439117038
21	24.507473055945287	27.644667806535146	29.897862972169463	17.949996165350104
22	22.80173055494151	24.330402457785016	30.881337886792114	21.986529100481363
23	21.191854301349345	26.871647500936106	30.622611802601245	21.3138863951133
24	21.644794124414087	26.28675060790479	31.77300677154058	20.29544849614054
25	20.91643621172681	27.45316087935289	30.24997405972129	21.38042884919901
26	20.156498829303942	29.278003094788037	29.46138958689542	21.1041084890126
27	19.950555573099706	29.074315514993483	30.718929185294797	20.25619972661202
28	18.713542630028467	27.124508826461792	32.98498170646432	21.176966837045423
29	19.497615750034964	25.818021049972256	32.40820524850787	22.27615795148491
30	21.620883954471427	26.49878419041518	31.87654231874512	20.00378953636827
31	23.84701100318953	26.239155835660437	29.249581572026003	20.66425158912403
32	22.853611112364263	27.593463952035297	28.279640715861465	21.273284219738972
33	23.302265150250605	26.868038418680612	27.890987670472743	21.93870876059604
34	21.19726792473259	26.789540879623573	30.784343801175655	21.22884739446818
35	20.516504784289666	28.471824345966624	29.014088954854895	21.997581914888816
36	23.589638324844472	28.01888452290188	28.426485250131954	19.96499190212169
37	21.864948141999342	28.576036596094074	29.09687227909033	20.462142982816257
38	21.487347911018077	29.6641748961261	26.92172351723111	21.92675367562471
39	22.468116013949103	26.142838452966892	29.588609736401654	21.80043579668235
40	22.90887518440155	26.007272300744827	30.133355589340578	20.950496925513054
41	21.027189923442343	27.471431858271337	28.694234039961565	22.807144178324755
42	21.974799583151	26.66570424473187	28.74859584143497	22.610900330682163
43	22.71488701316864	26.14441742645367	28.12264563774739	23.018049922630297
44	21.637350392262128	26.459986556168598	29.07724789432607	22.825415157243203
45	20.986587748068015	27.304060668672715	28.41723697685225	23.292114606407026
46	22.751654538646505	27.134208235023436	27.845648574638076	22.268488651691985
47	20.938992975823663	26.397504319620325	30.355990850976482	22.307511853579534
48	21.386068040223222	26.71307344933525	29.868539178843562	22.032319331597964
49	22.368189549000057	25.078159187595585	31.067205622950155	21.486445640454203
50	20.55237003920366	26.271412008318933	30.54050518128871	22.635712771188697
51	20.688387326707662	26.367729391012485	28.666489220122436	24.27739406215742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	134.0
1	381.0
2	628.0
3	3333.0
4	6038.0
5	4050.0
6	2062.0
7	1932.5
8	1803.0
9	1822.0
10	1841.0
11	1808.5
12	1776.0
13	1693.5
14	1611.0
15	1553.0
16	1495.0
17	1541.0
18	1587.0
19	1504.5
20	1422.0
21	1834.0
22	2246.0
23	2493.5
24	2741.0
25	3305.5
26	4351.0
27	4832.0
28	5424.0
29	6016.0
30	7422.0
31	8828.0
32	9851.5
33	10875.0
34	13131.0
35	15387.0
36	16163.0
37	16939.0
38	18298.0
39	19657.0
40	22107.0
41	24557.0
42	25921.0
43	27285.0
44	29254.0
45	31223.0
46	31579.0
47	31935.0
48	35476.0
49	39017.0
50	37761.0
51	36505.0
52	35463.5
53	34422.0
54	30745.0
55	27068.0
56	23476.0
57	19884.0
58	18655.0
59	17426.0
60	15381.5
61	13337.0
62	11595.0
63	9853.0
64	8221.0
65	6589.0
66	5577.0
67	4565.0
68	3804.0
69	3043.0
70	2303.0
71	1563.0
72	1494.5
73	1426.0
74	1122.5
75	665.5
76	512.0
77	397.5
78	283.0
79	200.0
80	117.0
81	86.0
82	55.0
83	38.5
84	22.0
85	22.5
86	23.0
87	13.5
88	4.0
89	4.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443326.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.38954372580403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48342127852082	23.380006090696117
2	9.081082406251499	5.701020665373222
3	3.459831910226597	3.2580763508996955
4	2.029914414552207	2.548723491009055
5	1.3970054985821596	2.192568259146668
6	1.0719849933234997	2.0189471892800244
7	0.7893981664371571	1.734519378371407
8	0.5649252770219352	1.4186197347913592
9	0.4985481581516899	1.408427928874938
>10	6.246410244247694	46.43263719453052
>50	0.34361992429504795	6.411494834343362
>100	0.03025584239075894	1.6240162199426544
>500	7.203771997799748E-4	0.20690265124582694
>1k	0.0028815087991198992	1.664040011495126
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3454	0.7791106319051894	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGC	1528	0.34466735539986376	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	1306	0.2945913391048574	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	1071	0.2415829434772605	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	915	0.20639439148617497	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10534008833228821	0.0
2	0.0	0.0	0.0	0.5560242349873457	0.0
3	0.0	0.0	0.0	0.7085079602820498	0.0
4	0.0	0.0	0.0	0.9992646494904427	0.0
5	0.0	0.0	0.0	1.8503313588645827	0.0
6	0.0	0.0	0.0	2.2344730514339335	0.0
7	0.0	0.0	0.0	2.6413970757411023	0.0
8	0.0	0.0	0.0	3.3061449136752636	0.0
9	0.0	0.0	0.0	3.532163689925698	0.0
10	0.0	0.0	0.0	4.379395749403374	0.0
11	0.0	0.0	0.0	5.1654989781785865	0.0
12	0.0	0.0	0.0	6.162733518900312	0.0
13	0.0	0.0	0.0	6.460031669696792	0.0
14	0.0	0.0	0.0	6.594469983714016	0.0
15	0.0	0.0	0.0	6.871241479182363	0.0
16	0.0	0.0	0.0	7.3632045041346545	0.0
17	0.0	0.0	0.0	7.920131009685875	0.0
18	0.0	0.0	0.0	8.535705101888903	0.0
19	0.0	0.0	0.0	8.933380852916365	0.0
20	0.0	0.0	0.0	9.315943571998936	0.0
21	0.0	0.0	0.0	9.765951015731087	0.0
22	0.0	0.0	0.0	10.238064088278152	0.0
23	0.0	0.0	0.0	10.719876569386862	0.0
24	0.0	0.0	0.0	11.116650049850449	0.0
25	0.0	0.0	0.0	11.457708322994817	0.0
26	0.0	0.0	0.0	11.783879131835263	0.0
27	2.2556764096849722E-4	0.0	0.0	12.112080049444426	0.0
28	2.2556764096849722E-4	0.0	0.0	12.44930367269233	0.0
29	2.2556764096849722E-4	0.0	0.0	12.812693142292579	0.0
30	2.2556764096849722E-4	0.0	0.0	13.247136418797904	0.0
31	2.2556764096849722E-4	0.0	0.0	13.63375935541791	0.0
32	2.2556764096849722E-4	0.0	0.0	14.00008120435075	0.0
33	2.2556764096849722E-4	0.0	0.0	14.381064949946541	0.0
34	4.5113528193699444E-4	0.0	0.0	14.736559552112892	0.0
35	4.5113528193699444E-4	0.0	0.0	15.151604011494927	0.0
36	4.5113528193699444E-4	0.0	0.0	15.50709861366128	0.0
37	4.5113528193699444E-4	0.0	0.0	15.889435765102881	0.0
38	4.5113528193699444E-4	0.0	0.0	16.274930863518044	0.0
39	4.5113528193699444E-4	0.0	0.0	16.7091485723824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	25	3.8869148E-5	45.0	29
ACGCACG	20	7.028593E-4	45.0	1
TAGCGGT	20	7.028593E-4	45.0	39
TAGCGGC	20	7.028593E-4	45.0	3
CACGACA	20	7.028593E-4	45.0	34
ATCACTA	20	7.028593E-4	45.0	26
TAACGCC	20	7.028593E-4	45.0	12
TCGATCA	20	7.028593E-4	45.0	17
ATCCGTT	20	7.028593E-4	45.0	16
TGTTGCG	20	7.028593E-4	45.0	1
TACGCAA	20	7.028593E-4	45.0	41
CTACGCA	20	7.028593E-4	45.0	40
CCTTTCG	35	1.2096643E-7	45.0	11
GGACGTA	25	3.8869148E-5	45.0	8
CGTAATC	20	7.028593E-4	45.0	21
ATAACGC	20	7.028593E-4	45.0	11
TAGTGCG	35	1.2096643E-7	45.0	1
GCGTATT	20	7.028593E-4	45.0	31
AAATGCG	25	3.8869148E-5	45.0	33
CAACACG	30	2.1624728E-6	44.999996	22
>>END_MODULE