##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936607.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571279
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20385835992571	31.0	31.0	33.0	30.0	34.0
2	31.243285680026748	31.0	31.0	34.0	28.0	34.0
3	31.005746754212915	31.0	31.0	34.0	28.0	34.0
4	34.99079258996042	35.0	35.0	37.0	32.0	37.0
5	35.20408416903124	35.0	35.0	37.0	33.0	37.0
6	35.16461133701746	36.0	35.0	37.0	32.0	37.0
7	30.67477012107919	35.0	35.0	37.0	0.0	37.0
8	32.805909196732244	35.0	35.0	37.0	17.0	37.0
9	36.07406538661495	39.0	34.0	39.0	32.0	39.0
10	36.13451045811241	37.0	35.0	39.0	32.0	39.0
11	36.371214415373224	37.0	35.0	39.0	32.0	39.0
12	36.380689645514714	38.0	35.0	39.0	32.0	39.0
13	36.20761834410157	38.0	35.0	39.0	32.0	39.0
14	37.3119509031489	39.0	36.0	40.0	32.0	41.0
15	37.37528948202192	39.0	36.0	41.0	32.0	41.0
16	37.4489032504258	39.0	36.0	40.0	33.0	41.0
17	37.215288851856975	39.0	35.0	40.0	32.0	41.0
18	37.160200182397745	39.0	35.0	40.0	32.0	41.0
19	36.953432560972836	39.0	35.0	40.0	31.0	41.0
20	36.87990106410353	39.0	35.0	40.0	31.0	41.0
21	36.99482564561274	39.0	35.0	40.0	32.0	41.0
22	37.07148521125404	39.0	35.0	40.0	32.0	41.0
23	37.15081772653992	39.0	35.0	40.0	32.0	41.0
24	37.07541323941542	39.0	35.0	40.0	32.0	41.0
25	36.8459124175753	39.0	35.0	40.0	31.0	41.0
26	36.72959272089469	38.0	35.0	40.0	31.0	41.0
27	36.770702231309045	39.0	35.0	40.0	31.0	41.0
28	36.70910710878572	39.0	35.0	40.0	31.0	41.0
29	36.65885845620091	39.0	35.0	40.0	31.0	41.0
30	36.4670047384903	38.0	35.0	40.0	31.0	41.0
31	36.46802175469429	38.0	35.0	40.0	31.0	41.0
32	36.26853253839192	38.0	35.0	40.0	30.0	41.0
33	36.13502159190168	38.0	35.0	40.0	30.0	41.0
34	35.986451453667996	38.0	35.0	40.0	30.0	41.0
35	35.912116496492956	38.0	35.0	40.0	29.0	41.0
36	35.86508168513108	38.0	35.0	40.0	29.0	41.0
37	35.78797050127871	38.0	35.0	40.0	29.0	41.0
38	35.67891695651337	38.0	35.0	40.0	29.0	41.0
39	35.60308710805053	38.0	35.0	40.0	28.0	41.0
40	35.509633646607	38.0	35.0	40.0	28.0	41.0
41	35.339067250852914	38.0	34.0	40.0	27.0	41.0
42	35.36888455553241	38.0	34.0	40.0	27.0	41.0
43	35.29708601226371	38.0	34.0	40.0	27.0	41.0
44	35.129933009965356	38.0	34.0	40.0	26.0	41.0
45	35.05084380836684	38.0	34.0	40.0	26.0	41.0
46	34.88088482160205	37.0	34.0	40.0	25.0	41.0
47	34.818325196620215	37.0	34.0	40.0	25.0	41.0
48	34.72272917436139	37.0	34.0	40.0	25.0	41.0
49	34.61694898639719	37.0	34.0	40.0	24.0	41.0
50	34.4784912450834	37.0	34.0	40.0	24.0	41.0
51	32.6688115614262	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	14.0
9	37.0
10	47.0
11	28.0
12	30.0
13	21.0
14	34.0
15	47.0
16	108.0
17	160.0
18	361.0
19	586.0
20	1024.0
21	1603.0
22	2264.0
23	3116.0
24	4229.0
25	5634.0
26	7159.0
27	8409.0
28	9298.0
29	10955.0
30	13359.0
31	17384.0
32	23017.0
33	32660.0
34	50959.0
35	52275.0
36	57075.0
37	82443.0
38	105264.0
39	81673.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42351460494785	19.43113609987414	22.28665853287098	12.858690762307035
2	32.441766632416034	28.244868094223662	23.366341139793338	15.947024133566961
3	30.976283042086266	28.116559509451598	26.521016876167337	14.386140572294797
4	28.235765711675032	23.066312607324967	30.907314989698552	17.79060669130145
5	29.75761405547902	27.626781310007893	24.008234155290147	18.607370479222936
6	25.561065608923133	36.96985185872402	24.034315982208344	13.4347665501445
7	64.36924865083435	18.704170816711276	13.4755522257951	3.4510283066592677
8	74.89545388505441	10.449010028374929	10.378991701077757	4.276544385492903
9	68.81576252584114	7.778160933624376	12.085513383128035	11.32056315740645
10	43.823770872025754	23.774723033754086	18.586364981033785	13.815141113186376
11	35.87476521979628	23.549614111493682	23.538411179126136	17.037209489583898
12	33.170307327943085	19.588677336292776	27.498647771054074	19.74236756471006
13	26.223438985154363	22.263027347408183	30.723691926361724	20.789841741075726
14	20.790541924348698	27.279140314977447	27.933461583569496	23.996856177104355
15	19.945420713871854	24.464928695085938	36.31745609413264	19.272194496909567
16	23.20424871210039	23.560992089679473	30.762026960556927	22.47273223766321
17	22.258651201952112	24.183980156806044	30.512236577924273	23.04513206331757
18	23.39539874562167	25.245282952812897	28.613689633261508	22.74562866830393
19	25.40194896014032	27.645686258378134	25.915883482501545	21.036481298980007
20	28.24714368986082	25.16878793024074	29.25033127421102	17.333737105687412
21	25.82030846574091	28.834597455884076	26.410562964856048	18.93453111351896
22	22.96111007056097	24.026789012023897	29.125698651622063	23.886402265793073
23	24.030289928388758	27.58161948890122	28.59706028052843	19.791030302181596
24	26.29783345790761	22.22749304630487	27.4893703426872	23.985303153100325
25	22.41794289655317	27.974422305038342	26.848352556281608	22.759282242126876
26	21.708132103578112	25.63370962349395	29.09314012942888	23.56501814349906
27	23.998081497832057	26.90961859266663	26.87355915410859	22.218740755392723
28	19.75969710071611	27.562014357258015	31.028621741740903	21.649666800284976
29	24.270277745199806	25.28956954482836	29.216897522926626	21.223255187045208
30	25.348559985576223	24.604090120589063	29.581342916508397	20.466006977326316
31	27.958493135578237	24.017511583657022	25.8141818621024	22.209813418662335
32	27.93293644611477	27.03057525307249	26.622893542384716	18.41359475842802
33	27.340756442998952	24.595862967131644	25.31232550119994	22.75105508866946
34	26.054869862186425	24.84250252503593	29.54808421104224	19.554543401735405
35	24.46562887835891	23.89340409852279	27.22662656950457	24.414340453613733
36	24.67340826461326	28.272350287687804	27.438782101214994	19.615459346483945
37	25.913957978500875	26.640398124209014	28.318212292067447	19.127431605222668
38	27.235903997871443	26.683284349678527	24.087529910954192	21.99328174149584
39	26.841700815188375	23.710656264277176	28.118835105088756	21.328807815445693
40	26.22623971824625	23.501126419840393	27.864843622818274	22.407790239095082
41	22.34529888198236	26.234291825885425	28.966231911202755	22.45417738092946
42	22.2329194666704	24.855981053040633	28.679506860920846	24.23159261936812
43	23.85576924760056	24.728022559905057	27.760866406781975	23.655341785712412
44	24.085779452771764	24.49206079691359	27.995427803227496	23.426731947087152
45	23.643788761708375	23.440210475091856	26.877235116291686	26.03876564690808
46	24.92267350979119	27.638684425648414	25.32615412084113	22.11248794371927
47	21.83801610071436	24.77300933519349	31.741933451080822	21.64704111301133
48	23.619282347154368	24.33329423976726	27.832110054806847	24.21531335827153
49	22.021814209869433	23.072089119326982	31.09041291558065	23.81568375522293
50	21.32740744889975	24.118688066601432	28.78593471841254	25.767969766086274
51	20.958410864043664	23.74741588610819	27.522278956516864	27.77189429333128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	236.0
1	675.0
2	1114.0
3	4009.5
4	6905.0
5	4731.0
6	2557.0
7	2315.5
8	2074.0
9	2005.5
10	1937.0
11	1873.5
12	1810.0
13	1859.5
14	1909.0
15	1823.0
16	1737.0
17	1756.5
18	1776.0
19	1665.0
20	1554.0
21	1859.5
22	2165.0
23	2248.5
24	2332.0
25	2805.5
26	4093.0
27	4907.0
28	5691.5
29	6476.0
30	7688.5
31	8901.0
32	10069.5
33	11238.0
34	12789.5
35	14341.0
36	15134.0
37	15927.0
38	17259.5
39	18592.0
40	20445.5
41	22299.0
42	24105.5
43	25912.0
44	27755.5
45	29599.0
46	31922.0
47	34245.0
48	50308.0
49	66371.0
50	53135.5
51	39900.0
52	39348.0
53	38796.0
54	35879.5
55	32963.0
56	33244.5
57	33526.0
58	33139.0
59	32752.0
60	32559.5
61	32367.0
62	28980.0
63	25593.0
64	21030.0
65	16467.0
66	13143.5
67	9820.0
68	8519.5
69	7219.0
70	5980.5
71	4742.0
72	3654.0
73	2566.0
74	2250.0
75	1547.0
76	1160.0
77	868.5
78	577.0
79	430.0
80	283.0
81	260.0
82	237.0
83	153.5
84	70.0
85	66.0
86	62.0
87	46.0
88	30.0
89	23.5
90	17.0
91	9.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571279.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.84096372674178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38416442883128	21.898541888380084
2	8.488377707353337	5.066027425280289
3	3.6254590376199554	3.2456157490321575
4	2.23356672008385	2.666071339011191
5	1.5380250943061153	2.2948075525003695
6	1.1306247877660995	2.024335996816998
7	0.90479114181362	1.889988775019626
8	0.7230538437797527	1.7261298819770252
9	0.5955505021022952	1.59946208376097
>10	7.156416051205759	45.618546914384055
>50	0.16731812752517108	3.1036497814962942
>100	0.044462159761933545	2.380547216811984
>500	0.003510170507521069	0.8845873238182987
>1k	0.002925142089600891	1.6387647935035266
>5k	0.0017550852537605345	3.9629232782071013
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	8546	1.4959415627040378	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	8255	1.4450032295953465	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	5899	1.032595281815015	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3373	0.5904295449333863	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	2213	0.3873763957715932	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCT	1490	0.2608182691819584	No Hit
GAACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCT	1289	0.22563405971513045	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCG	1022	0.17889682624426945	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTC	993	0.17382049751522463	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTA	968	0.16944435205915148	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGC	943	0.16506820660307836	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	868	0.15193977023485897	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	683	0.11955629385991783	No Hit
GAATCTATCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTC	612	0.10712804076467015	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07702016002688704	0.0
2	0.0	0.0	0.0	0.6886302489676673	0.0
3	0.0	0.0	0.0	0.8102870926465002	0.0
4	0.0	0.0	0.0	1.1952128469626926	0.0
5	0.0	0.0	0.0	2.743318063503122	0.0
6	0.0	0.0	0.0	3.12141703090784	0.0
7	0.0	0.0	0.0	3.645854302363644	0.0
8	0.0	0.0	0.0	4.264291178215898	0.0
9	0.0	0.0	0.0	4.446688920825026	0.0
10	0.0	0.0	0.0	6.620057800129183	0.0
11	0.0	0.0	0.0	7.262475953080719	0.0
12	0.0	0.0	0.0	9.485557844765868	0.0
13	0.0	0.0	0.0	9.799414996875432	0.0
14	0.0	0.0	0.0	9.956606141657579	0.0
15	0.0	0.0	0.0	10.346783270521058	0.0
16	0.0	0.0	0.0	10.774945341943253	0.0
17	0.0	0.0	0.0	11.19242961845263	0.0
18	0.0	0.0	0.0	11.663302869526099	0.0
19	0.0	0.0	0.0	12.049278898751748	0.0
20	0.0	0.0	0.0	12.365761738134957	0.0
21	0.0	0.0	0.0	12.719179245167423	0.0
22	0.0	0.0	0.0	13.102704632937671	0.0
23	0.0	0.0	0.0	13.453146361060007	0.0
24	0.0	0.0	0.0	13.743897465161506	0.0
25	0.0	0.0	0.0	14.003665459434007	0.0
26	0.0	0.0	0.0	14.245053642791001	0.0
27	0.0	0.0	0.0	14.513748973793891	0.0
28	1.7504581824292508E-4	0.0	0.0	14.773867059702877	0.0
29	1.7504581824292508E-4	0.0	0.0	15.034510283066593	0.0
30	1.7504581824292508E-4	0.0	0.0	15.345216610447785	0.0
31	5.251374547287752E-4	0.0	0.0	15.643669730551972	0.0
32	5.251374547287752E-4	0.0	0.0	15.913765428100806	0.0
33	5.251374547287752E-4	0.0	0.0	16.187887179469225	0.0
34	5.251374547287752E-4	0.0	0.0	16.475487458842352	0.0
35	5.251374547287752E-4	0.0	0.0	16.787244061133002	0.0
36	5.251374547287752E-4	0.0	0.0	17.08499699796422	0.0
37	5.251374547287752E-4	0.0	0.0	17.390802042434608	0.0
38	5.251374547287752E-4	0.0	0.0	17.703608919634714	0.0
39	5.251374547287752E-4	0.0	0.0	18.05230018957462	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGTT	20	7.0303027E-4	45.000004	19
GTATACG	20	7.0303027E-4	45.000004	1
TCGAGTG	20	7.0303027E-4	45.000004	1
CACGTCA	40	6.8048394E-9	45.000004	34
GCGCTTA	20	7.0303027E-4	45.000004	24
CACCGAA	20	7.0303027E-4	45.000004	16
GCATCCG	20	7.0303027E-4	45.000004	16
CCTCGAC	25	3.8883336E-5	45.0	30
CGGGCCA	25	3.8883336E-5	45.0	6
GCCTAAC	25	3.8883336E-5	45.0	16
CTCGACT	25	3.8883336E-5	45.0	31
TATAGCG	25	3.8883336E-5	45.0	1
CGCACGG	25	3.8883336E-5	45.0	2
TCGACTA	25	3.8883336E-5	45.0	32
TACGAAT	130	0.0	43.269234	12
CGTTTTA	130	0.0	43.269234	1
TACGGCT	1095	0.0	40.68493	7
TGATAGT	50	1.0786607E-9	40.5	32
ATCTACC	50	1.0786607E-9	40.5	15
CGTTTTT	1880	0.0	40.332447	1
>>END_MODULE