FastQCFastQC Report
Sat 14 Jan 2017
SRR2936606.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936606.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405691
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193121.3738438959913664No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT26050.1853181104524394No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC18890.13438230734919696No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG16520.11752227196446445Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC16300.11595720538866651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC207.033824E-445.034
CGTTTTT74900.043.2877161
ATGTCGC250.002107268735.99999633
TCGATCA1000.035.99999617
TATCCGC250.002107268735.99999611
TACGGGA3450.035.8695644
CACGACC1700.035.73529427
CGTTCTG3400.035.073531
CTTCGCA458.701754E-735.024
GTTTTTT97550.034.9436232
AATAGCG653.6743586E-1034.6153831
GCGAGAC1650.034.0909121
AACCGAC606.4337655E-933.7541
GACCGAT4300.033.4883739
CGTTTTA5650.033.0530971
TCGTTAG754.7293724E-1133.0000041
ATGCGAG1300.032.8846131
CCTCGAT1100.032.72727615
CCAGTAG4200.032.14285726
TAACGGG3800.031.9736843
Produced by FastQC (version 0.11.5)