##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936604.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	477241
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.120239040652418	31.0	31.0	33.0	28.0	34.0
2	31.153440295364398	31.0	31.0	34.0	28.0	34.0
3	30.931248572524154	31.0	31.0	34.0	28.0	34.0
4	34.92973571005006	35.0	35.0	37.0	32.0	37.0
5	35.13256824120308	35.0	35.0	37.0	33.0	37.0
6	35.08332896796377	35.0	35.0	37.0	32.0	37.0
7	30.822173702594707	35.0	35.0	37.0	0.0	37.0
8	32.81810238432993	35.0	33.0	37.0	17.0	37.0
9	35.91273172254689	38.0	34.0	39.0	32.0	39.0
10	36.02270970013054	37.0	35.0	39.0	32.0	39.0
11	36.27935361798337	37.0	35.0	39.0	32.0	39.0
12	36.33985973543765	38.0	35.0	39.0	32.0	39.0
13	36.12559272987861	38.0	35.0	39.0	31.0	39.0
14	37.27839393513969	39.0	36.0	40.0	32.0	41.0
15	37.3509400910651	39.0	36.0	41.0	32.0	41.0
16	37.475485132249744	39.0	36.0	40.0	32.0	41.0
17	37.21248174402451	39.0	36.0	40.0	32.0	41.0
18	37.1427161538929	39.0	36.0	40.0	32.0	41.0
19	36.94011201887516	39.0	35.0	40.0	31.0	41.0
20	36.86614729245811	39.0	35.0	40.0	31.0	41.0
21	37.04711875132271	39.0	35.0	40.0	32.0	41.0
22	37.077505914202675	39.0	35.0	40.0	32.0	41.0
23	37.15484210283693	39.0	35.0	40.0	32.0	41.0
24	37.008280931437156	39.0	35.0	40.0	31.0	41.0
25	36.828872624103965	39.0	35.0	40.0	31.0	41.0
26	36.684651989246525	38.0	35.0	40.0	31.0	41.0
27	36.69880207274731	39.0	35.0	40.0	31.0	41.0
28	36.65967090002745	39.0	35.0	40.0	31.0	41.0
29	36.57094214453494	39.0	35.0	40.0	31.0	41.0
30	36.42054643251523	38.0	35.0	40.0	30.0	41.0
31	36.44275114669527	38.0	35.0	40.0	30.0	41.0
32	36.23185769873083	38.0	35.0	40.0	30.0	41.0
33	36.00453858742229	38.0	35.0	40.0	30.0	41.0
34	35.96890879031768	38.0	35.0	40.0	29.0	41.0
35	35.645912652098204	38.0	35.0	40.0	27.0	41.0
36	35.71706538206064	38.0	35.0	40.0	28.0	41.0
37	35.587026261364805	38.0	35.0	40.0	27.0	41.0
38	35.478064541814305	38.0	34.0	40.0	27.0	41.0
39	35.48941310574741	38.0	34.0	40.0	27.0	41.0
40	35.13885227798953	38.0	34.0	40.0	26.0	41.0
41	34.99955158923898	38.0	34.0	40.0	25.0	41.0
42	34.93959026990556	38.0	34.0	40.0	25.0	41.0
43	34.94009735123345	38.0	34.0	40.0	25.0	41.0
44	34.858298008762866	38.0	34.0	40.0	25.0	41.0
45	34.70771371277824	37.0	34.0	40.0	24.0	41.0
46	34.51837541200358	37.0	33.0	40.0	24.0	41.0
47	34.28126250678378	37.0	33.0	40.0	23.0	41.0
48	34.24431262192477	36.0	33.0	40.0	24.0	41.0
49	34.1081969068039	36.0	33.0	40.0	24.0	41.0
50	33.93163202658615	36.0	33.0	40.0	23.0	41.0
51	32.04240624757722	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	21.0
9	44.0
10	27.0
11	32.0
12	24.0
13	20.0
14	22.0
15	43.0
16	61.0
17	149.0
18	327.0
19	558.0
20	1009.0
21	1462.0
22	2037.0
23	2802.0
24	3931.0
25	5316.0
26	6576.0
27	7819.0
28	8869.0
29	10087.0
30	12333.0
31	15343.0
32	20102.0
33	26820.0
34	39827.0
35	43440.0
36	48403.0
37	67912.0
38	86309.0
39	65510.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.42656226099602	18.7037576402698	21.091859249310097	12.777820849424085
2	30.74379611139864	31.358160761543957	21.456664452551227	16.441378674506172
3	30.248239359149782	30.29873795419924	25.43348119713101	14.019541489519971
4	26.895845076177444	23.156225051912973	32.44251855980521	17.505411312104364
5	31.11090622976651	26.64062811032581	23.29137689343539	18.957088766472285
6	24.24959297294239	38.55976330617026	24.324607483430803	12.866036237456546
7	62.25345265809099	18.11830919807812	15.924029997422688	3.704208146408209
8	70.81495512749323	12.625486913320524	11.688224607692968	4.871333351493271
9	65.3051602858933	7.888048176916904	12.93602184221389	13.870769694975913
10	41.42121066714721	23.469693509149465	20.382993078968486	14.72610274473484
11	33.69450654910203	24.655467572987234	24.337389285497267	17.312636592413476
12	30.568203486288898	20.51961168466247	28.43950959787613	20.47267523117251
13	24.138328433642542	23.49127589624529	33.069245936539396	19.301149733572764
14	19.979842469527973	29.27305072280043	27.544364377746255	23.20274242992534
15	18.090231141079663	23.806211117653344	38.310203859266075	19.793353882000915
16	20.064286178262137	25.016082021452473	31.180891834523855	23.738739965761532
17	19.681670267223478	24.10878361247252	32.3901341251066	23.819411995197395
18	20.89636053901488	26.01411027133042	29.81135317376336	23.27817601589134
19	23.6834220027198	27.156719560976526	26.4713216173799	22.688536818923772
20	26.632875213990413	24.47107436284812	31.01933823791334	17.876712185248124
21	24.16514926420823	29.0817427672811	27.190245599183644	19.56286236932703
22	21.5972642752823	23.982432355979473	28.778541659245537	25.641761709492688
23	22.897236406763042	29.12302170182361	28.526048683998233	19.45369320741512
24	23.75655067355906	23.157691816084537	28.193093217053857	24.892664293302545
25	21.491447717191104	29.867299750021477	26.39316404080957	22.248088491977846
26	21.636657370175655	26.60060640221607	28.70331761101833	23.05941861658994
27	23.93423867605675	28.43636653179421	25.509543396313394	22.119851395835646
28	20.23757388824514	26.8711196229997	31.553659471839175	21.33764701691598
29	27.03979750272923	24.464578692945494	26.7692842819456	21.72633952237968
30	21.919952393025746	28.900702160962698	28.36910491764119	20.810240528370365
31	28.97529759597352	24.510886533219065	24.08028647999648	22.433529390810932
32	28.601063194486642	27.661915049209938	25.139709287341194	18.597312468962222
33	23.990394790053664	26.03296866782192	23.787981334378227	26.18865520774619
34	29.933723213219317	24.625294138600832	25.807506060879092	19.633476587300756
35	22.24347866172437	26.711032790560747	27.042731031072353	24.002757516642532
36	26.987203530291826	29.598043755670616	23.508667528565233	19.90608518547233
37	26.090382008251595	28.781265649849868	26.92853296342938	18.199819378469158
38	23.97467945964408	26.585729222761668	24.216695547951662	25.22289576964259
39	29.996794072596444	23.66309684205674	25.083134097866694	21.25697498748012
40	23.667497134571423	23.554975368838804	30.53342022164902	22.244107274940752
41	22.523840156231337	29.219199523930257	25.919189675656533	22.33777064418187
42	22.47920861786812	24.95510653946329	31.322120270471316	21.243564572197275
43	24.744102036497285	27.274479770179006	24.460387938169603	23.521030255154106
44	24.11276482950962	25.438091027384484	27.3126156386396	23.1365285044663
45	22.884664142435373	23.470741197843438	26.54885058073384	27.095744078987344
46	25.135937608042898	27.978945648005933	24.54692702429171	22.33818971965946
47	21.215696052937613	24.825821754627118	32.79160843263676	21.166873759798506
48	24.396898003314888	25.322426195569953	26.294890841314977	23.985784959800185
49	21.17797925995461	23.941153421436965	31.159099909689235	23.721767408919185
50	21.934620034741357	24.023920828260774	28.888968047590215	25.152491089407658
51	22.03184554554198	23.545546170593052	26.03778803581419	28.384820248050772
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	495.5
2	834.0
3	3375.5
4	5917.0
5	4063.5
6	2210.0
7	2015.5
8	1821.0
9	1738.0
10	1655.0
11	1611.5
12	1568.0
13	1502.0
14	1436.0
15	1481.5
16	1527.0
17	1492.0
18	1457.0
19	1522.5
20	1588.0
21	1939.5
22	2291.0
23	2203.0
24	2115.0
25	2348.0
26	3224.0
27	3867.0
28	4651.5
29	5436.0
30	6872.5
31	8309.0
32	9499.5
33	10690.0
34	11040.0
35	11390.0
36	12374.0
37	13358.0
38	14146.5
39	14935.0
40	16894.0
41	18853.0
42	20702.5
43	22552.0
44	24890.0
45	27228.0
46	27681.0
47	28134.0
48	47043.0
49	65952.0
50	50750.5
51	35549.0
52	34900.5
53	34252.0
54	30331.5
55	26411.0
56	25148.5
57	23886.0
58	23635.5
59	23385.0
60	23019.0
61	22653.0
62	20354.0
63	18055.0
64	14833.0
65	11611.0
66	9463.0
67	7315.0
68	6535.5
69	5756.0
70	4778.0
71	3800.0
72	3392.5
73	2985.0
74	2367.0
75	1349.0
76	949.0
77	743.0
78	537.0
79	384.5
80	232.0
81	165.0
82	98.0
83	85.5
84	73.0
85	59.5
86	46.0
87	34.5
88	23.0
89	14.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	477241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.26985880806963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47198050943452	22.54256335177896
2	8.657837039036764	5.241430095098369
3	3.5015908001325244	3.1797797737094124
4	2.093567946309544	2.534880245595607
5	1.4266406348199843	2.1592105292927877
6	1.0453925664778534	1.8986331231774098
7	0.8218620651000987	1.7414354069201963
8	0.6299019345385476	1.52536340971294
9	0.5320163744577489	1.4493654484575444
>10	6.59619912330065	43.272648257339256
>50	0.16760454517643933	3.243599896056102
>100	0.04571033050266527	2.490561450694577
>500	0.0027703230607675922	0.6178185686192692
>1k	0.004848065356343286	2.543810828512458
>5k	0.0013851615303837961	3.4553395480090927
>10k+	6.925807651918981E-4	2.1035600670260424
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	10034	2.102501671063467	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	9335	1.9560347916461496	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	7147	1.4975662191639025	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.6826739529923037	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2259	0.4733457519366525	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	1850	0.3876448167697243	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	1284	0.2690464566120681	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTA	1237	0.2591981828887292	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1220	0.25563604132922363	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	1026	0.21498572000310115	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	983	0.20597559723494002	No Hit
GAATCTATCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	772	0.16176313434931197	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	623	0.13054201126893958	TruSeq Adapter, Index 14 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	569	0.11922697337403954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0873772370772838	0.0
2	0.0	0.0	0.0	0.8888590879660381	0.0
3	0.0	0.0	0.0	1.0659184772473447	0.0
4	0.0	0.0	0.0	1.5434549839598861	0.0
5	0.0	0.0	0.0	3.7285145241083644	0.0
6	0.0	0.0	0.0	4.132503284504056	0.0
7	0.0	0.0	0.0	4.8627423042026985	0.0
8	0.0	0.0	0.0	5.70131233485807	0.0
9	0.0	0.0	0.0	5.903935328272299	0.0
10	0.0	0.0	0.0	8.846054718685108	0.0
11	0.0	0.0	0.0	9.533128964192096	0.0
12	0.0	0.0	0.0	12.442350929614179	0.0
13	0.0	0.0	0.0	12.805270293206158	0.0
14	0.0	0.0	0.0	13.005797909232442	0.0
15	0.0	0.0	0.0	13.52272751083834	0.0
16	0.0	0.0	0.0	14.014512583788903	0.0
17	0.0	0.0	0.0	14.476543297830656	0.0
18	0.0	0.0	0.0	14.988863069183076	0.0
19	0.0	0.0	0.0	15.373155282132089	0.0
20	0.0	0.0	0.0	15.713654107673062	0.0
21	0.0	0.0	0.0	16.09585094323413	0.0
22	0.0	0.0	0.0	16.515135958561817	0.0
23	0.0	0.0	0.0	16.893561114824585	0.0
24	0.0	0.0	0.0	17.215411081612853	0.0
25	0.0	0.0	0.0	17.498077491246562	0.0
26	0.0	0.0	0.0	17.762304579866356	0.0
27	0.0	0.0	0.0	18.064877074685537	0.0
28	0.0	0.0	0.0	18.345657644670094	0.0
29	0.0	0.0	0.0	18.62769544108742	0.0
30	0.0	0.0	0.0	18.976994851657757	0.0
31	0.0	0.0	0.0	19.278519657782965	0.0
32	0.0	0.0	0.0	19.576482322348667	0.0
33	0.0	0.0	0.0	19.851186297908185	0.0
34	0.0	0.0	0.0	20.13531947171345	0.0
35	0.0	0.0	0.0	20.458007589456898	0.0
36	0.0	0.0	0.0	20.782162471371908	0.0
37	0.0	0.0	0.0	21.099821683384285	0.0
38	0.0	0.0	0.0	21.434034376761428	0.0
39	0.0	0.0	0.0	21.748969598169477	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAT	30	2.1628239E-6	45.000004	30
TCGTTCA	20	7.0291356E-4	45.0	16
GGGTCGA	25	3.887366E-5	45.0	7
CGACCCT	25	3.887366E-5	45.0	12
TAACGCC	25	3.887366E-5	45.0	12
CGTTCAT	20	7.0291356E-4	45.0	17
GCCGATT	35	1.2099372E-7	45.0	9
ATATCGT	20	7.0291356E-4	45.0	30
CCGTACT	25	3.887366E-5	45.0	33
AGTTGCG	25	3.887366E-5	45.0	1
TGACACG	20	7.0291356E-4	45.0	12
TTGCGTG	20	7.0291356E-4	45.0	1
ACGTCCG	20	7.0291356E-4	45.0	1
CGCAATT	25	3.887366E-5	45.0	31
GTAAGCG	20	7.0291356E-4	45.0	1
GGCGAAT	25	3.887366E-5	45.0	8
CGATCCT	20	7.0291356E-4	45.0	10
TAAGACG	20	7.0291356E-4	45.0	1
TAATGAC	35	1.2099372E-7	45.0	9
ACGAATT	20	7.0291356E-4	45.0	8
>>END_MODULE