##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936603.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	384673
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.096861490148775	31.0	31.0	33.0	28.0	34.0
2	31.14044136188399	31.0	31.0	34.0	28.0	34.0
3	30.8828147543498	31.0	31.0	34.0	27.0	34.0
4	34.88180870505598	35.0	35.0	37.0	32.0	37.0
5	35.11818609572286	35.0	35.0	37.0	33.0	37.0
6	35.10116384565592	36.0	35.0	37.0	32.0	37.0
7	30.98439973691941	35.0	35.0	37.0	0.0	37.0
8	32.99039703852363	35.0	35.0	37.0	17.0	37.0
9	36.151892646481556	39.0	35.0	39.0	32.0	39.0
10	36.03147868449306	37.0	35.0	39.0	32.0	39.0
11	36.3205137870347	37.0	35.0	39.0	32.0	39.0
12	36.393271687901155	38.0	35.0	39.0	32.0	39.0
13	36.26880753263161	38.0	35.0	39.0	32.0	39.0
14	37.38219474722686	39.0	36.0	40.0	32.0	41.0
15	37.5843794599569	39.0	36.0	41.0	32.0	41.0
16	37.54636015524874	39.0	36.0	41.0	33.0	41.0
17	37.39676296490786	39.0	36.0	40.0	32.0	41.0
18	37.32971640848201	39.0	36.0	40.0	32.0	41.0
19	37.09909455563557	39.0	36.0	40.0	32.0	41.0
20	37.03395351376364	39.0	35.0	40.0	32.0	41.0
21	37.07082898981732	39.0	35.0	40.0	32.0	41.0
22	37.13509916214551	39.0	35.0	40.0	32.0	41.0
23	37.20276962511016	39.0	35.0	40.0	32.0	41.0
24	37.15037447390381	39.0	35.0	40.0	32.0	41.0
25	36.805957267601315	39.0	35.0	40.0	31.0	41.0
26	36.777582518138786	39.0	35.0	40.0	31.0	41.0
27	36.79269405443065	39.0	35.0	40.0	31.0	41.0
28	36.74262815430249	39.0	35.0	40.0	31.0	41.0
29	36.67066053505185	39.0	35.0	40.0	31.0	41.0
30	36.499068039607664	39.0	35.0	40.0	30.0	41.0
31	36.46954946149067	39.0	35.0	40.0	30.0	41.0
32	36.28200055631666	38.0	35.0	40.0	30.0	41.0
33	36.16205452423227	38.0	35.0	40.0	30.0	41.0
34	36.08492147876248	39.0	35.0	40.0	30.0	41.0
35	35.956121173048274	38.0	35.0	40.0	29.0	41.0
36	35.930109469601454	38.0	35.0	40.0	29.0	41.0
37	35.77599415607542	38.0	35.0	40.0	28.0	41.0
38	35.650755316853534	38.0	35.0	40.0	27.0	41.0
39	35.56695946947147	38.0	35.0	40.0	27.0	41.0
40	35.44140347775903	38.0	34.0	40.0	26.0	41.0
41	35.28729856267531	38.0	34.0	40.0	26.0	41.0
42	35.29051688057129	38.0	34.0	40.0	26.0	41.0
43	35.249128480553615	38.0	34.0	40.0	26.0	41.0
44	35.06357867591435	38.0	34.0	40.0	26.0	41.0
45	34.993802528381245	38.0	34.0	40.0	25.0	41.0
46	34.88726268804933	38.0	34.0	40.0	25.0	41.0
47	34.82275075193736	38.0	34.0	40.0	24.0	41.0
48	34.74952232155623	37.0	34.0	40.0	24.0	41.0
49	34.59477530265966	37.0	34.0	40.0	24.0	41.0
50	34.436570801693904	37.0	33.0	40.0	24.0	41.0
51	32.70463224608954	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	28.0
10	38.0
11	27.0
12	19.0
13	20.0
14	22.0
15	28.0
16	56.0
17	103.0
18	239.0
19	443.0
20	662.0
21	1133.0
22	1666.0
23	2308.0
24	3124.0
25	4188.0
26	5349.0
27	6082.0
28	6722.0
29	7505.0
30	9010.0
31	11388.0
32	14930.0
33	19895.0
34	30503.0
35	33506.0
36	37999.0
37	55613.0
38	73346.0
39	58706.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48004668900599	20.655985733337147	22.666264593563884	13.197702984092983
2	33.61062512835577	26.266205322442698	23.44458800071749	16.678581548484036
3	31.545494484926156	25.86326568280072	27.15163268542372	15.439607146849402
4	29.13929493361894	25.20712397282887	28.063836037361607	17.589745056190583
5	26.99279647908743	29.583308420398623	23.992585910630588	19.431309189883354
6	25.529475684542458	36.68908397522051	24.59023638258989	13.191203957647144
7	66.22845897684527	18.577857037015853	11.400592191289745	3.7930917948491314
8	75.88081305420448	9.030527227021393	10.658143410117164	4.430516308656964
9	68.68379116808302	9.687188859108906	13.13947170713827	8.489548265669802
10	39.80497721441328	25.934235051589273	20.426440119270133	13.834347614727314
11	31.437090723809575	26.66784515679551	25.38571721956051	16.509346899834405
12	29.71250906614189	23.030989957704335	29.18322835239281	18.07327262376096
13	23.426910648784812	26.989157024277766	30.46665609491698	19.117276232020444
14	19.505398091365915	30.548543828134544	28.467295599119254	21.47876248138029
15	18.789725299150188	26.943923800214726	36.100792101343224	18.165558799291865
16	20.840558084398957	25.891601438104573	32.724417882201244	20.543422595295226
17	20.757370545892226	26.12062713005592	29.17958889758314	23.942413426468715
18	22.17779776589467	26.5615210841416	30.127406914444215	21.13327423551952
19	24.66900458311345	26.685782469786023	27.44538868077562	21.199824266324903
20	25.348542788290313	26.09359118004123	29.536255468930754	19.0216105627377
21	23.797875078313268	27.73004603910334	29.156972285551625	19.315106597031765
22	21.92875507249014	25.35946115271932	28.7886074666015	23.923176308189035
23	22.034559223028392	27.00189511611161	28.835920379127206	22.127625281732797
24	22.217831768801034	24.523946312842337	30.41726349392861	22.84095842442802
25	20.423580547633964	29.27629441109722	28.14650365375267	22.15362138751615
26	20.28736095332919	28.2723248057441	28.67240487374991	22.7679093671768
27	21.882741965253604	29.10342030763792	27.83507030646809	21.178767420640387
28	20.396804558677108	27.89694103823247	31.00607528992157	20.700179113168847
29	22.210552859181696	25.128355772305312	30.328356812149536	22.332734556363455
30	21.436388828953422	26.670704728431684	29.227941654340178	22.664964788274716
31	24.64898758166027	26.185617394514303	26.976938854559585	22.188456169265844
32	23.214522464534813	28.381508450034186	27.692091724659644	20.711877360771354
33	22.362630078014316	27.411853704315092	25.79515588564833	24.430360332022264
34	22.723196065229427	26.110488648800413	29.126816802842935	22.03949848312723
35	21.567149241043694	26.32365671622391	28.0126237089684	24.096570333764003
36	24.03755917363579	28.67578436750175	25.82375160201002	21.462904856852443
37	21.529454887657828	28.307679509609464	30.286243120780508	19.876622481952204
38	21.868184146014926	27.49842073657366	26.957181814164237	23.676213303247174
39	22.301279268365597	25.104959277100292	30.642649731070286	21.951111723463825
40	22.327275374148954	26.716457874610384	29.17958889758314	21.77667785365752
41	19.456005490377542	28.409844205338043	28.893631733966252	23.240518570318166
42	21.742103032965662	27.4654056822288	28.90377021522176	21.888721069583777
43	23.022671203853662	27.033870326225127	27.45136778510579	22.492090684815413
44	21.650336779550422	26.618712516864974	28.320157640385467	23.410793063199133
45	20.77842739157674	26.85371731314649	27.320867334073355	25.04698796120341
46	22.92258619658775	28.097630974879962	26.767930164061422	22.211852664470864
47	20.685621293930172	26.12140701322942	31.560312265222667	21.632659427617742
48	21.731184668536656	26.410223748482476	29.060526733095383	22.79806484988549
49	20.503648553446695	26.684482664496855	30.206175114967778	22.60569366708867
50	20.29308009660153	24.991876216942703	30.227491921710126	24.48755176474564
51	20.059115144551345	26.09905036225573	27.954392432013687	25.887442061179232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	431.5
2	703.0
3	2599.0
4	4495.0
5	3138.5
6	1782.0
7	1668.0
8	1554.0
9	1528.5
10	1503.0
11	1424.5
12	1346.0
13	1301.5
14	1257.0
15	1231.0
16	1205.0
17	1166.0
18	1127.0
19	1117.5
20	1108.0
21	1563.5
22	2019.0
23	2054.0
24	2089.0
25	2587.5
26	3513.0
27	3940.0
28	5526.5
29	7113.0
30	7457.0
31	7801.0
32	8763.0
33	9725.0
34	10713.5
35	11702.0
36	12971.0
37	14240.0
38	14552.5
39	14865.0
40	16783.5
41	18702.0
42	21168.5
43	23635.0
44	25701.0
45	27767.0
46	34038.0
47	40309.0
48	35495.0
49	30681.0
50	28771.5
51	26862.0
52	23754.5
53	20647.0
54	19237.5
55	17828.0
56	17203.0
57	16578.0
58	16404.0
59	16230.0
60	15548.5
61	14867.0
62	13474.0
63	12081.0
64	10597.0
65	9113.0
66	7302.5
67	5492.0
68	4744.0
69	3996.0
70	3275.5
71	2555.0
72	2262.5
73	1970.0
74	1471.5
75	816.0
76	659.0
77	528.0
78	397.0
79	299.5
80	202.0
81	147.5
82	93.0
83	84.0
84	75.0
85	79.0
86	83.0
87	60.5
88	38.0
89	24.5
90	11.0
91	9.5
92	8.0
93	4.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	384673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.81034626597633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.19383716184922	23.919419971858627
2	8.493753716791218	5.403784936581042
3	3.4465116865917236	3.289041904806505
4	1.964048331227249	2.4990822999780704
5	1.3787024699309696	2.1928501483130476
6	1.0070626459918317	1.9221006888318306
7	0.7678902464934303	1.7098798244655353
8	0.6377065371795425	1.6228532611006363
9	0.4248618600014632	1.2163502593667972
>10	6.450682966162959	46.43943089663225
>50	0.18991857716045304	3.73133070824634
>100	0.03765626960940017	2.4272268498544305
>500	0.0032744582269043625	0.6770507252034674
>1k	0.004093072783630454	2.9495975247614137
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCG	2817	0.7323102999170724	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2596	0.6748589061358609	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGC	2562	0.6660202301695206	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTC	2258	0.5869920685881256	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	1094	0.284397397269889	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	820	0.21316806742349997	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTT	706	0.18353250683047678	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	569	0.1479178419072823	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCT	505	0.13128033420593596	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	447	0.11620259285159083	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	420	0.10918364429008534	No Hit
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	414	0.10762387794308412	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCT	391	0.10164477361291278	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	389	0.10112485149724572	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.199221156670731E-4	0.0	0.0	0.12088189189259449	0.0
2	5.199221156670731E-4	0.0	0.0	0.7164526753892267	0.0
3	5.199221156670731E-4	0.0	0.0	0.914283040400548	0.0
4	7.798831735006096E-4	0.0	0.0	1.256391792509482	0.0
5	7.798831735006096E-4	0.0	0.0	2.5361800802239824	0.0
6	7.798831735006096E-4	0.0	0.0	3.115893239192769	0.0
7	7.798831735006096E-4	0.0	0.0	3.614498548117492	0.0
8	7.798831735006096E-4	0.0	0.0	4.363446355735911	0.0
9	7.798831735006096E-4	0.0	0.0	4.580513839026914	0.0
10	7.798831735006096E-4	0.0	0.0	5.938290444091475	0.0
11	7.798831735006096E-4	0.0	0.0	6.704395681526908	0.0
12	7.798831735006096E-4	0.0	0.0	8.115724264505177	0.0
13	7.798831735006096E-4	0.0	0.0	8.51294476087482	0.0
14	7.798831735006096E-4	0.0	0.0	8.715194463869313	0.0
15	7.798831735006096E-4	0.0	0.0	9.044825085202236	0.0
16	7.798831735006096E-4	0.0	0.0	9.579045059050154	0.0
17	7.798831735006096E-4	0.0	0.0	10.123663475211414	0.0
18	7.798831735006096E-4	0.0	0.0	10.777205574604924	0.0
19	7.798831735006096E-4	0.0	0.0	11.125813353159696	0.0
20	7.798831735006096E-4	0.0	0.0	11.49807758797732	0.0
21	7.798831735006096E-4	0.0	0.0	11.935592048311163	0.0
22	7.798831735006096E-4	0.0	0.0	12.3676473264305	0.0
23	0.0010398442313341461	0.0	0.0	12.787744395889495	0.0
24	0.0010398442313341461	0.0	0.0	13.132972680692433	0.0
25	0.0010398442313341461	0.0	0.0	13.456364236637352	0.0
26	0.0010398442313341461	0.0	0.0	13.740501672849406	0.0
27	0.0010398442313341461	0.0	0.0	14.069872333124499	0.0
28	0.0010398442313341461	0.0	0.0	14.374546692905403	0.0
29	0.0010398442313341461	0.0	0.0	14.679481013744141	0.0
30	0.0010398442313341461	0.0	0.0	15.050445443272597	0.0
31	0.0010398442313341461	0.0	0.0	15.378516298258521	0.0
32	0.0010398442313341461	0.0	0.0	15.723484622003625	0.0
33	0.0010398442313341461	0.0	0.0	16.042716801023207	0.0
34	0.0010398442313341461	0.0	0.0	16.382485903611638	0.0
35	0.0010398442313341461	0.0	0.0	16.736032942265247	0.0
36	0.0010398442313341461	0.0	0.0	17.08983994197669	0.0
37	0.0012998052891676827	0.0	0.0	17.443646941688137	0.0
38	0.0012998052891676827	0.0	0.0	17.76443888705472	0.0
39	0.0012998052891676827	0.0	0.0	18.11876580888183	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATCGC	20	7.027427E-4	45.000004	24
GCACGTC	20	7.027427E-4	45.000004	23
CACGATA	20	7.027427E-4	45.000004	27
CGACAGG	30	2.1617216E-6	45.000004	2
TACGCGC	20	7.027427E-4	45.000004	34
CCATCGA	20	7.027427E-4	45.000004	31
GTCGTTT	20	7.027427E-4	45.000004	33
GACGGTA	20	7.027427E-4	45.000004	32
CACACGC	20	7.027427E-4	45.000004	26
CCTATCG	20	7.027427E-4	45.000004	23
ATGTTCG	20	7.027427E-4	45.000004	40
GGTTAAC	20	7.027427E-4	45.000004	8
TAGTCGG	20	7.027427E-4	45.000004	2
ACGGTAT	20	7.027427E-4	45.000004	33
ACGAAAG	20	7.027427E-4	45.000004	1
GCGAGAC	35	1.2091186E-7	45.0	21
CGGTTTA	25	3.8859493E-5	45.0	1
GCGCAAA	25	3.8859493E-5	45.0	40
GTAATCG	35	1.2091186E-7	45.0	40
GTTCACG	25	3.8859493E-5	45.0	1
>>END_MODULE