##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936601.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	816628
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209281337402096	31.0	31.0	33.0	30.0	34.0
2	31.236967138035922	31.0	31.0	34.0	28.0	34.0
3	31.030158162590556	31.0	31.0	34.0	28.0	34.0
4	35.006976248671364	35.0	35.0	37.0	32.0	37.0
5	35.22028512370382	35.0	35.0	37.0	33.0	37.0
6	35.17953217376823	36.0	35.0	37.0	32.0	37.0
7	30.999114651959033	35.0	35.0	37.0	0.0	37.0
8	33.00656724971468	35.0	35.0	37.0	17.0	37.0
9	36.20938175031961	39.0	35.0	39.0	32.0	39.0
10	36.1194472881165	37.0	35.0	39.0	32.0	39.0
11	36.405013053679276	38.0	35.0	39.0	32.0	39.0
12	36.51425985883413	38.0	35.0	39.0	32.0	39.0
13	36.45768330255637	39.0	35.0	39.0	32.0	39.0
14	37.58692574832114	39.0	36.0	41.0	33.0	41.0
15	37.704585196686864	39.0	36.0	41.0	33.0	41.0
16	37.67139750290218	39.0	36.0	41.0	33.0	41.0
17	37.50853754708386	39.0	36.0	41.0	32.0	41.0
18	37.42472459920551	39.0	36.0	40.0	32.0	41.0
19	37.24891505067178	39.0	36.0	40.0	32.0	41.0
20	37.234291501148626	39.0	35.0	40.0	32.0	41.0
21	37.3117049623574	39.0	35.0	40.0	32.0	41.0
22	37.39571996061854	39.0	36.0	40.0	32.0	41.0
23	37.45051724897995	39.0	36.0	41.0	33.0	41.0
24	37.35199748232978	39.0	35.0	41.0	32.0	41.0
25	37.07241485719324	39.0	35.0	40.0	32.0	41.0
26	37.01221608859848	39.0	35.0	40.0	31.0	41.0
27	37.061701288714076	39.0	35.0	40.0	32.0	41.0
28	37.04757613013514	39.0	35.0	40.0	32.0	41.0
29	37.02245453254113	39.0	35.0	40.0	31.0	41.0
30	36.83346027811929	39.0	35.0	40.0	31.0	41.0
31	36.84414935564296	39.0	35.0	40.0	31.0	41.0
32	36.716263708812335	39.0	35.0	40.0	31.0	41.0
33	36.59632537703826	39.0	35.0	40.0	30.0	41.0
34	36.51844168948407	39.0	35.0	40.0	30.0	41.0
35	36.44197725280054	39.0	35.0	40.0	30.0	41.0
36	36.42403762790401	39.0	35.0	40.0	30.0	41.0
37	36.361457113887845	39.0	35.0	40.0	30.0	41.0
38	36.25179273794188	39.0	35.0	40.0	30.0	41.0
39	36.20393618636638	39.0	35.0	40.0	30.0	41.0
40	36.03856590761032	38.0	35.0	40.0	29.0	41.0
41	35.87584432569052	38.0	35.0	40.0	29.0	41.0
42	35.892940237170414	38.0	35.0	40.0	29.0	41.0
43	35.86383396111816	38.0	35.0	40.0	29.0	41.0
44	35.70555136488095	38.0	35.0	40.0	28.0	41.0
45	35.63344754282244	38.0	35.0	40.0	28.0	41.0
46	35.52125325117434	38.0	34.0	40.0	28.0	41.0
47	35.447208520893234	38.0	34.0	40.0	27.0	41.0
48	35.37232007719549	38.0	34.0	40.0	27.0	41.0
49	35.26253324647208	38.0	34.0	40.0	27.0	41.0
50	35.09598495275695	38.0	34.0	40.0	26.0	41.0
51	33.413167317309714	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	31.0
9	64.0
10	76.0
11	66.0
12	41.0
13	32.0
14	43.0
15	70.0
16	98.0
17	226.0
18	423.0
19	732.0
20	1256.0
21	1826.0
22	2654.0
23	3687.0
24	4957.0
25	6676.0
26	8773.0
27	10107.0
28	11853.0
29	13958.0
30	17268.0
31	22274.0
32	29161.0
33	40953.0
34	62446.0
35	69878.0
36	82695.0
37	122730.0
38	164823.0
39	136740.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.2187532144379	19.999804072356078	22.43836851050907	12.343074202696943
2	32.65452568366502	26.952908790783564	23.63536396008954	16.757201565461873
3	32.56623578912308	26.36586058768497	25.662725255563117	15.405178367628835
4	29.352532609707232	24.10191666217666	28.07790083122303	18.467649896893075
5	27.24263679423189	28.755810479190036	24.281435365919364	19.72011736065871
6	25.79999706108534	36.18012118124776	24.35184686295351	13.668034894713383
7	66.05933178877041	18.313969151192463	11.58165039650857	4.045048663528559
8	76.46811032685629	8.853847774017055	9.971859892141833	4.706182006984821
9	69.74117958238023	8.765802789030012	11.877990957939232	9.61502667065053
10	40.659516940393914	24.695822332812494	19.154988562723783	15.489672164069809
11	31.16853695930093	25.454797043451855	25.146211004276118	18.230454992971097
12	28.769525414264514	22.044064127117853	28.838589908746698	20.34782054987093
13	24.351969317730962	23.363881718481363	31.46463261117669	20.819516352610982
14	20.421293416341346	27.739680735904233	29.117052072669562	22.721973775084862
15	19.93429076641996	25.779792022805974	34.96659433671145	19.319322874062607
16	22.53657724202452	24.98579524581572	30.56838609501511	21.90924141714465
17	22.148885416615645	24.865054835249342	30.196735845452277	22.78932390268274
18	22.11814926747552	26.587503734870708	29.668221025975107	21.626125971678658
19	24.46548489642775	27.410889658449133	26.615056059797116	21.508569385326
20	26.07221403135822	25.79632341776182	28.774056241030188	19.35740630984977
21	25.264992138403287	27.60179665649476	27.630451074418218	19.50276013068374
22	23.447028512370384	24.058077851849312	28.233908217695202	24.260985418085102
23	23.588341325548477	27.061526178382323	28.287298500663706	21.062833995405498
24	24.005054933213167	24.375235725446593	28.433631959717275	23.186077381622965
25	22.957454312122533	27.684331176496517	26.673099624308744	22.685114887072206
26	21.583879073458174	27.045851966868632	28.39346679271345	22.976802166959743
27	22.90173738838247	27.81621497181091	27.262719377733802	22.019328262072815
28	20.724124080976893	27.561998853823283	30.498586871868223	21.2152901933316
29	22.757730570100463	26.03670214589752	28.980392541034593	22.225174742967422
30	22.761771577756335	27.256964003193623	29.006965227741393	20.97429919130865
31	23.800555454870516	26.707386961994935	27.23920806046327	22.252849522671276
32	24.397155130610265	29.119990987328382	26.663915515999943	19.818938366061413
33	24.49940486978159	26.941887860813	25.61043706559168	22.94827020381373
34	24.300783220756575	26.822249543243682	28.5184931204906	20.358474115509143
35	22.277095568606516	27.85184931204906	26.91862145309737	22.952433666247053
36	22.37959021733274	28.02426563869963	27.653472572578945	21.942671571388686
37	22.298157790328034	30.189755923137586	27.334967696429707	20.177118590104676
38	23.219997354976808	27.73784391424247	26.305245472846877	22.736913257933843
39	25.09490245252428	26.313694852490976	26.302918832075317	22.288483862909427
40	24.49952732455904	25.342628467307023	29.436659041815854	20.72118516631808
41	21.49081344259565	27.177368397850678	28.02793928202315	23.30387887753053
42	21.48897662093389	27.699882933232757	28.694215726132338	22.116924719701014
43	23.199914771474894	26.483049809705278	26.737021018138	23.58001440068183
44	22.57429331347933	25.884368402748866	28.04422576742409	23.497112516347713
45	21.99128611803661	25.871020832006742	27.060424085385264	25.077268964571388
46	23.46111081177721	27.55048810474292	26.735918925140943	22.25248215833893
47	22.013327977977735	25.636397478411222	30.776191852348926	21.574082691262117
48	22.010389063318918	25.784690213904003	28.231214212591286	23.97370651018579
49	20.959727072792017	25.578231459122147	29.8624343030119	23.599607165073937
50	20.998300327688984	25.286789088789508	29.248813413206502	24.466097170315003
51	20.928256194987192	24.936813334835445	28.11953545555626	26.015395014621102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	312.0
1	896.0
2	1480.0
3	4712.5
4	7945.0
5	5358.0
6	2771.0
7	2574.5
8	2378.0
9	2324.5
10	2271.0
11	2175.5
12	2080.0
13	2073.0
14	2066.0
15	1989.0
16	1912.0
17	2010.0
18	2108.0
19	2187.5
20	2267.0
21	2771.5
22	3276.0
23	3851.5
24	4427.0
25	4910.0
26	6314.0
27	7235.0
28	9240.0
29	11245.0
30	13216.0
31	15187.0
32	17517.0
33	19847.0
34	22154.0
35	24461.0
36	25704.5
37	26948.0
38	29440.0
39	31932.0
40	36322.0
41	40712.0
42	44604.0
43	48496.0
44	67368.0
45	86240.0
46	74016.5
47	61793.0
48	61736.0
49	61679.0
50	55140.0
51	48601.0
52	45107.5
53	41614.0
54	39860.0
55	38106.0
56	38370.0
57	38634.0
58	38334.0
59	38034.0
60	38237.0
61	38440.0
62	34973.0
63	31506.0
64	26963.5
65	22421.0
66	18750.0
67	15079.0
68	12193.5
69	9308.0
70	7913.5
71	6519.0
72	5588.0
73	4657.0
74	4012.0
75	2503.0
76	1639.0
77	1396.5
78	1154.0
79	846.0
80	538.0
81	407.5
82	277.0
83	191.0
84	105.0
85	88.5
86	72.0
87	60.5
88	49.0
89	34.0
90	19.0
91	15.0
92	11.0
93	11.0
94	11.0
95	8.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	816628.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.89029161863771
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93072053254258	21.799205046750668
2	8.888001852981091	5.313299345851942
3	3.840429651380042	3.443746866618379
4	2.3223802003622507	2.7766648575271176
5	1.5656811189216813	2.339933261318202
6	1.1362952008545502	2.037851695104061
7	0.8763164524255714	1.8335348019247273
8	0.6770631590708649	1.6190092215091396
9	0.5431194239767211	1.4610598169769653
>10	6.963133012248782	46.54796786276944
>50	0.21134000913154571	3.906709021295842
>100	0.038203770480395555	2.062262251687741
>500	0.0032513847217357913	0.6629220727768617
>1k	0.0028449616315188173	1.6173986580449211
>5k	0.0012192692706509216	2.578435219844033
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG	7385	0.9043285314733269	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGC	7108	0.8704085581194865	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	6732	0.8243655617980281	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4379	0.5362294704565604	No Hit
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	2279	0.2790744378101167	TruSeq Adapter, Index 15 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	1579	0.19335609359463551	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT	1436	0.1758450604191872	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	1270	0.15551756736237307	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1219	0.14927237371238802	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTC	1152	0.14106790362319196	No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	821	0.1005353722870144	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09233090219781835	0.0
2	0.0	0.0	0.0	0.6063960579358043	0.0
3	0.0	0.0	0.0	0.7613013514109239	0.0
4	0.0	0.0	0.0	1.1537689131403772	0.0
5	0.0	0.0	0.0	2.544242911092933	0.0
6	0.0	0.0	0.0	3.103493879710223	0.0
7	0.0	0.0	0.0	3.6130282086825334	0.0
8	0.0	0.0	0.0	4.342246408401377	0.0
9	0.0	0.0	0.0	4.594870614282145	0.0
10	0.0	0.0	0.0	6.116126314552036	0.0
11	0.0	0.0	0.0	6.7644019063759755	0.0
12	0.0	0.0	0.0	8.28822915697233	0.0
13	0.0	0.0	0.0	8.609060673893131	0.0
14	0.0	0.0	0.0	8.769476432353532	0.0
15	0.0	0.0	0.0	9.080511567078277	0.0
16	0.0	0.0	0.0	9.495510807858658	0.0
17	0.0	0.0	0.0	9.953124311191877	0.0
18	0.0	0.0	0.0	10.459229906395569	0.0
19	0.0	0.0	0.0	10.786061707411452	0.0
20	0.0	0.0	0.0	11.08999446504406	0.0
21	0.0	0.0	0.0	11.447439960422615	0.0
22	0.0	0.0	0.0	11.836968607493253	0.0
23	0.0	0.0	0.0	12.20372066595806	0.0
24	0.0	0.0	0.0	12.528960554867087	0.0
25	0.0	0.0	0.0	12.826280754517358	0.0
26	0.0	0.0	0.0	13.086619611377518	0.0
27	0.0	0.0	0.0	13.350142292451398	0.0
28	0.0	0.0	0.0	13.642931665335992	0.0
29	0.0	0.0	0.0	13.931680030564712	0.0
30	0.0	0.0	0.0	14.27161449276782	0.0
31	0.0	0.0	0.0	14.57775143639454	0.0
32	0.0	0.0	0.0	14.900542229754551	0.0
33	0.0	0.0	0.0	15.215373462580269	0.0
34	0.0	0.0	0.0	15.520653222764834	0.0
35	0.0	0.0	0.0	15.848832026332675	0.0
36	0.0	0.0	0.0	16.176153646458364	0.0
37	0.0	0.0	0.0	16.491964517503686	0.0
38	0.0	0.0	0.0	16.81879631851957	0.0
39	0.0	0.0	0.0	17.150648765410935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	45	3.8380676E-10	45.0	2
CGTATAG	20	7.032087E-4	45.0	2
TTCGCAC	20	7.032087E-4	45.0	22
CGTCCAT	20	7.032087E-4	45.0	29
GCTACGA	195	0.0	43.846153	10
TACGAAT	205	0.0	41.707317	12
CGAATAT	210	0.0	40.714287	14
TCACGAC	90	0.0	40.0	25
AGGCGAT	130	0.0	39.80769	7
CGTTTTT	1850	0.0	39.77027	1
AACGGCT	80	0.0	39.375	7
TGATACC	975	0.0	39.0	4
ATGGTCG	35	6.246066E-6	38.57143	44
ATAACGC	35	6.246066E-6	38.57143	11
TACGGCT	905	0.0	38.28729	7
ACGGTCT	95	0.0	37.894737	30
GTATCGC	30	1.1396325E-4	37.499996	5
GCGATAT	30	1.1396325E-4	37.499996	9
ACGCTAG	30	1.1396325E-4	37.499996	1
TACGTTC	30	1.1396325E-4	37.499996	37
>>END_MODULE