##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936600.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560773
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.995252981152802	31.0	30.0	33.0	28.0	34.0
2	31.023235783463186	31.0	31.0	34.0	28.0	34.0
3	30.730327601364547	31.0	30.0	34.0	27.0	34.0
4	34.71091332856611	35.0	35.0	37.0	32.0	37.0
5	34.98202302892614	35.0	35.0	37.0	32.0	37.0
6	34.93566559017642	35.0	35.0	37.0	32.0	37.0
7	30.68760978149804	35.0	35.0	37.0	0.0	37.0
8	32.71651095897984	35.0	33.0	37.0	17.0	37.0
9	35.868970153698555	38.0	34.0	39.0	32.0	39.0
10	35.8788368912198	37.0	35.0	39.0	32.0	39.0
11	36.15398922558683	37.0	35.0	39.0	32.0	39.0
12	36.26350769384403	37.0	35.0	39.0	32.0	39.0
13	36.06549709062312	38.0	35.0	39.0	31.0	39.0
14	37.14097326369137	39.0	36.0	40.0	32.0	41.0
15	37.19326358437371	39.0	36.0	40.0	32.0	41.0
16	37.31116334060306	39.0	36.0	40.0	32.0	41.0
17	37.064958548289596	39.0	35.0	40.0	31.0	41.0
18	36.9367373250852	39.0	35.0	40.0	31.0	41.0
19	36.68486178899484	38.0	35.0	40.0	31.0	41.0
20	36.533766782637535	38.0	35.0	40.0	31.0	41.0
21	36.705346013449294	38.0	35.0	40.0	31.0	41.0
22	36.81539589102899	38.0	35.0	40.0	31.0	41.0
23	36.89653389161033	38.0	35.0	40.0	32.0	41.0
24	36.73946142200142	38.0	35.0	40.0	31.0	41.0
25	36.547183976404	38.0	35.0	40.0	31.0	41.0
26	36.42865829845588	38.0	35.0	40.0	31.0	41.0
27	36.463390712462974	38.0	35.0	40.0	31.0	41.0
28	36.43291313954131	38.0	35.0	40.0	31.0	41.0
29	36.32179866006388	38.0	35.0	40.0	30.0	41.0
30	36.12770051339847	38.0	35.0	40.0	30.0	41.0
31	36.08321014028849	38.0	35.0	40.0	30.0	41.0
32	35.820601205835516	38.0	35.0	40.0	29.0	41.0
33	35.53237406223195	38.0	34.0	40.0	27.0	41.0
34	35.17777246764734	38.0	34.0	40.0	25.0	41.0
35	35.053504715811926	38.0	34.0	40.0	25.0	41.0
36	34.942780768688934	38.0	34.0	40.0	25.0	41.0
37	34.86596537279791	38.0	34.0	40.0	24.0	41.0
38	34.857519887726404	38.0	34.0	40.0	24.0	41.0
39	34.6037951185239	38.0	34.0	40.0	23.0	41.0
40	34.53255417076072	38.0	33.0	40.0	23.0	41.0
41	34.13310020275584	37.0	33.0	40.0	22.0	41.0
42	34.411276933803876	37.0	33.0	40.0	23.0	41.0
43	34.42091184846631	38.0	33.0	40.0	22.0	41.0
44	34.32742303926901	37.0	33.0	40.0	22.0	41.0
45	34.289072405411815	37.0	33.0	40.0	23.0	41.0
46	34.15137319378786	37.0	33.0	40.0	22.0	41.0
47	34.00385895897271	37.0	33.0	40.0	22.0	41.0
48	33.9639426291922	37.0	33.0	40.0	22.0	41.0
49	33.890815000008914	37.0	33.0	40.0	21.0	41.0
50	33.67989721331091	37.0	33.0	40.0	20.0	41.0
51	31.906650284517976	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	15.0
9	33.0
10	36.0
11	27.0
12	9.0
13	18.0
14	26.0
15	43.0
16	71.0
17	188.0
18	445.0
19	837.0
20	1447.0
21	2191.0
22	3101.0
23	4153.0
24	5860.0
25	8104.0
26	9834.0
27	10811.0
28	11279.0
29	12626.0
30	14929.0
31	19387.0
32	24532.0
33	32907.0
34	46568.0
35	51956.0
36	57792.0
37	79773.0
38	95474.0
39	66295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75531632229084	19.414807774268734	21.616411631801103	15.213464271639326
2	30.85526585623773	30.836898352809428	23.001999026343995	15.305836764608852
3	28.021855545826924	30.310125487496727	28.44020664332983	13.227812323346525
4	24.35655782286237	22.090400215416935	37.15924268821787	16.393799273502825
5	28.935059284238008	25.209844268536468	28.022390521654927	17.832705925570597
6	22.546199620880465	36.13333737537292	28.38153762752486	12.938925376221752
7	60.30033542984417	17.39955383015944	18.39657044829191	3.903540291704487
8	69.95968065509574	10.35659705442309	14.396734507545833	5.28698778293534
9	65.04075624183048	6.249409297523241	15.175480987850698	13.534353472795587
10	40.02082839223714	21.772267923027677	23.062451294908993	15.144452389826185
11	30.133940114805814	21.851266020296983	29.075936252280332	18.938857612616868
12	25.57701601182653	18.882685150675943	33.572408086694615	21.96789075080291
13	22.683153432850727	22.59327749374524	38.105614927965505	16.617954145438528
14	20.00363783563046	27.596549762559896	31.630980806850545	20.7688315949591
15	16.45514316844784	23.949084567195637	41.5678001615627	18.02797210279382
16	20.347983943592148	24.599615174054385	31.379185517134385	23.67321536521908
17	20.680917233889648	24.040208783233144	33.19739003126042	22.081483951616786
18	21.3922567598654	24.242429646220483	33.10590916467091	21.259404429243205
19	21.046662374971692	26.884140284928126	29.963996126775005	22.10520121332518
20	22.789791947900486	25.822213266330586	33.743778676933445	17.644216108835483
21	21.284013317331613	28.94772037883422	31.957316061935938	17.810950241898237
22	20.184994641325456	22.103774611117156	33.11839193399111	24.592838813566274
23	20.268094219942828	27.751157776854452	31.93948353433564	20.04126446886708
24	22.074172615300665	23.631665575910397	30.411770894818403	23.882390913970536
25	19.639497622032444	30.46758670620732	28.753880803819015	21.139034867941216
26	19.38199592348419	24.10280095511018	31.195153832299344	25.32004928910629
27	21.287223172299665	27.251847004046198	28.781164571047462	22.67976525260667
28	17.188416703371953	24.55146734953359	33.82990978524287	24.430206161851586
29	21.83789162459676	23.471351152783747	29.157074252861676	25.533682969757816
30	20.487255984150448	27.57194087447149	31.140051322014433	20.800751819363626
31	23.05995474104495	23.466536370331667	27.743668115262327	25.729840773361058
32	27.670198101549108	26.009989781961686	26.261963396953846	20.057848719535357
33	24.715883254008304	23.370597371842084	27.705684831473697	24.20783454267591
34	23.04176556289265	22.616638104901625	31.59941723299802	22.7421790992077
35	23.74401049979225	25.613929343959143	28.244583815554602	22.397476340694006
36	22.08362385492882	24.554677204501644	29.44382129667441	23.917877643895125
37	22.63571890943394	27.09581238754362	30.644485380002244	19.623983323020187
38	23.862418483058207	24.93397506655991	26.55138531990663	24.652221130475255
39	25.13726588120327	22.660149472246346	31.21904941928374	20.983535227266646
40	26.881822056340088	22.955456129307226	30.168713543626385	19.9940082707263
41	19.209733706865347	25.00851503192914	32.72019159267654	23.06155966852898
42	23.486508801243996	23.58547932942563	31.338884004757716	21.589127864572653
43	23.043548815652677	23.09223161600148	29.975943920267202	23.888275648078636
44	21.952376451790652	23.00485223076004	30.56602225856095	24.476749058888355
45	21.540088413671842	22.010688817043615	28.868722281564914	27.58050048771963
46	25.618209150583215	25.640321484807576	27.053192646578918	21.68827671803029
47	18.621438621331627	23.439074277827213	35.98104758966641	21.958439511174753
48	22.23056388235525	24.363155858074478	28.497270731650776	24.909009527919494
49	20.517749606346953	21.600897332788847	34.66215384834862	23.21919921251558
50	21.70557426980258	22.27782008049603	31.555727540377298	24.460878109324096
51	21.037211135343533	22.695457876894928	27.66217346412898	28.605157523632556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	459.0
2	756.0
3	5540.0
4	10324.0
5	7016.5
6	3709.0
7	3443.5
8	3178.0
9	3180.0
10	3182.0
11	3056.5
12	2931.0
13	2951.5
14	2972.0
15	2854.5
16	2737.0
17	2617.0
18	2497.0
19	2407.5
20	2318.0
21	2552.0
22	2786.0
23	2849.0
24	2912.0
25	3275.5
26	4365.5
27	5092.0
28	5919.5
29	6747.0
30	7969.5
31	9192.0
32	10059.0
33	10926.0
34	12910.5
35	14895.0
36	15740.0
37	16585.0
38	18146.5
39	19708.0
40	21157.5
41	22607.0
42	24781.0
43	26955.0
44	28932.0
45	30909.0
46	34250.0
47	37591.0
48	61881.0
49	86171.0
50	66215.0
51	46259.0
52	44262.0
53	42265.0
54	36242.5
55	30220.0
56	27566.0
57	24912.0
58	23092.5
59	21273.0
60	19528.5
61	17784.0
62	16002.0
63	14220.0
64	12569.5
65	10919.0
66	9023.0
67	7127.0
68	6059.5
69	4992.0
70	4307.5
71	3623.0
72	3035.0
73	2447.0
74	1953.0
75	1209.0
76	959.0
77	697.5
78	436.0
79	331.5
80	227.0
81	156.5
82	86.0
83	66.0
84	46.0
85	31.0
86	16.0
87	11.0
88	6.0
89	7.0
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	560773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.5888147733599
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.28337381630868	29.425792382149197
2	8.470278632673175	6.367754692046003
3	3.1308302498988314	3.5305259505083777
4	1.864059687299136	2.802711772494976
5	1.2491640753828483	2.3477298525550636
6	0.8361774984045355	1.8858552663107715
7	0.6766433378075338	1.7803954764732763
8	0.4923096987575284	1.4804270462180273
9	0.4113827956185089	1.3917052534905991
>10	4.444042534093616	34.56695082346729
>50	0.11348431686094396	2.660158546907867
>100	0.021190017671031024	1.6434607830156551
>500	9.417785631569344E-4	0.2853267401422979
>1k	0.004238003534206204	3.0212420145712673
>5k	4.708892815784672E-4	1.06059418544209
>10k+	0.0014126678447354015	5.74936921420727
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	11041	1.9688893723485261	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	10815	1.9285878599718604	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	10626	1.8948843828073034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5992	1.068525053809652	No Hit
GCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	3422	0.610229094482081	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT	2957	0.5273078411407112	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT	2074	0.36984662243010985	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTC	1722	0.3070761252770729	No Hit
GAATCTATCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTC	1596	0.2846071405007017	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT	1452	0.2589283007562775	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCG	1329	0.23699429180791518	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGGTGTTCGTA	1324	0.23610266542790043	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGC	1193	0.2127420542715145	No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT	822	0.14658337687442155	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC	790	0.1408769680423273	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.5665055200589187E-4	0.0	0.0	0.16976566275480454	0.0
2	3.5665055200589187E-4	0.0	0.0	1.1584009929151369	0.0
3	3.5665055200589187E-4	0.0	0.0	1.4137627881513553	0.0
4	3.5665055200589187E-4	0.0	0.0	2.27239899210554	0.0
5	3.5665055200589187E-4	0.0	0.0	5.00059738967461	0.0
6	3.5665055200589187E-4	0.0	0.0	5.816435527388087	0.0
7	3.5665055200589187E-4	0.0	0.0	6.671148575270207	0.0
8	3.5665055200589187E-4	0.0	0.0	7.827053014321303	0.0
9	3.5665055200589187E-4	0.0	0.0	8.147325210022593	0.0
10	3.5665055200589187E-4	0.0	0.0	11.031914874646247	0.0
11	3.5665055200589187E-4	0.0	0.0	11.965982670349678	0.0
12	3.5665055200589187E-4	0.0	0.0	15.084535097089196	0.0
13	3.5665055200589187E-4	0.0	0.0	15.595971988665646	0.0
14	3.5665055200589187E-4	0.0	0.0	15.838137713477646	0.0
15	3.5665055200589187E-4	0.0	0.0	16.351179532538122	0.0
16	3.5665055200589187E-4	0.0	0.0	16.897389852935145	0.0
17	3.5665055200589187E-4	0.0	0.0	17.520101716737432	0.0
18	3.5665055200589187E-4	0.0	0.0	18.207902306280793	0.0
19	3.5665055200589187E-4	0.0	0.0	18.712206186817127	0.0
20	3.5665055200589187E-4	0.0	0.0	19.13251886235607	0.0
21	3.5665055200589187E-4	0.0	0.0	19.634682839580364	0.0
22	3.5665055200589187E-4	0.0	0.0	20.179644883045366	0.0
23	3.5665055200589187E-4	0.0	0.0	20.717652240746254	0.0
24	3.5665055200589187E-4	0.0	0.0	21.12762205027703	0.0
25	3.5665055200589187E-4	0.0	0.0	21.484807578110928	0.0
26	3.5665055200589187E-4	0.0	0.0	21.856794103853073	0.0
27	3.5665055200589187E-4	0.0	0.0	22.27782008049603	0.0
28	3.5665055200589187E-4	0.0	0.0	22.643743546854076	0.0
29	3.5665055200589187E-4	0.0	0.0	23.045688718964715	0.0
30	3.5665055200589187E-4	0.0	0.0	23.52056892896056	0.0
31	3.5665055200589187E-4	0.0	0.0	23.962102312343855	0.0
32	3.5665055200589187E-4	0.0	0.0	24.360659304210436	0.0
33	3.5665055200589187E-4	0.0	0.0	24.74405864761677	0.0
34	3.5665055200589187E-4	0.0	0.0	25.159021564875626	0.0
35	3.5665055200589187E-4	0.0	0.0	25.567564772198377	0.0
36	3.5665055200589187E-4	0.0	0.0	25.953638994744754	0.0
37	3.5665055200589187E-4	0.0	0.0	26.3620038767915	0.0
38	3.5665055200589187E-4	0.0	0.0	26.78873626226655	0.0
39	3.5665055200589187E-4	0.0	0.0	27.298211575806967	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCT	20	7.0301915E-4	45.000004	14
CCTACGC	20	7.0301915E-4	45.000004	39
ATCCGTG	20	7.0301915E-4	45.000004	16
CGCTCCA	20	7.0301915E-4	45.000004	17
AAGTTCG	20	7.0301915E-4	45.000004	1
CTCTCTA	20	7.0301915E-4	45.000004	15
ATCGCAA	20	7.0301915E-4	45.000004	14
TCGATAC	25	3.888241E-5	45.0	40
GTATGCG	25	3.888241E-5	45.0	1
CAGTACG	25	3.888241E-5	45.0	43
TTCGATG	25	3.888241E-5	45.0	1
CGTTTTT	3175	0.0	43.228344	1
TACGGCT	1435	0.0	42.334496	7
ACGGCTG	1585	0.0	41.735016	8
GATGAAT	295	0.0	40.42373	20
CGGCTGT	1630	0.0	40.306747	9
CTATCTC	225	0.0	40.0	5
CGATGAA	310	0.0	39.919353	19
CGTTTTA	200	0.0	39.375	1
CCGATGA	320	0.0	38.67188	18
>>END_MODULE