##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936599.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	606028
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15330809797567	31.0	31.0	33.0	30.0	34.0
2	31.172419426165128	31.0	31.0	34.0	28.0	34.0
3	30.984008989683645	31.0	31.0	34.0	28.0	34.0
4	34.93439577049245	35.0	35.0	37.0	32.0	37.0
5	35.13366544120073	35.0	35.0	37.0	33.0	37.0
6	35.09179608862957	35.0	35.0	37.0	32.0	37.0
7	31.048250245863226	35.0	35.0	37.0	0.0	37.0
8	32.921208261004445	35.0	35.0	37.0	17.0	37.0
9	35.98175991868362	38.0	34.0	39.0	32.0	39.0
10	36.02297583609998	37.0	35.0	39.0	32.0	39.0
11	36.25687261974694	37.0	35.0	39.0	32.0	39.0
12	36.31829387421043	38.0	35.0	39.0	32.0	39.0
13	36.09018065171906	38.0	35.0	39.0	31.0	39.0
14	37.18084312936036	39.0	36.0	40.0	32.0	41.0
15	37.23692469654868	39.0	36.0	41.0	32.0	41.0
16	37.38900842865346	39.0	36.0	40.0	32.0	41.0
17	37.11345680397606	39.0	35.0	40.0	32.0	41.0
18	37.032590903390606	39.0	35.0	40.0	31.0	41.0
19	36.84749384516887	39.0	35.0	40.0	31.0	41.0
20	36.74425274079746	39.0	35.0	40.0	31.0	41.0
21	36.92699182216003	39.0	35.0	40.0	31.0	41.0
22	37.00753925561195	39.0	35.0	40.0	32.0	41.0
23	37.096045067224615	39.0	35.0	40.0	32.0	41.0
24	36.97907357415829	39.0	35.0	40.0	31.0	41.0
25	36.81481878725075	39.0	35.0	40.0	31.0	41.0
26	36.67569155220551	38.0	35.0	40.0	31.0	41.0
27	36.73491653850977	39.0	35.0	40.0	31.0	41.0
28	36.6742790762143	39.0	35.0	40.0	31.0	41.0
29	36.61326539367818	39.0	35.0	40.0	31.0	41.0
30	36.44382932801785	38.0	35.0	40.0	30.0	41.0
31	36.46650319787204	38.0	35.0	40.0	30.0	41.0
32	36.26261657877194	38.0	35.0	40.0	30.0	41.0
33	36.08730124680708	38.0	35.0	40.0	30.0	41.0
34	35.97167457609219	38.0	35.0	40.0	29.0	41.0
35	35.91407327714231	38.0	35.0	40.0	29.0	41.0
36	35.84071693057086	38.0	35.0	40.0	29.0	41.0
37	35.73799395407473	38.0	35.0	40.0	29.0	41.0
38	35.58987868547328	38.0	35.0	40.0	28.0	41.0
39	35.52281412740005	38.0	35.0	40.0	27.0	41.0
40	35.334101724672784	38.0	34.0	40.0	26.0	41.0
41	35.152888975426876	38.0	34.0	40.0	25.0	41.0
42	35.11508709168553	38.0	34.0	40.0	25.0	41.0
43	35.07005451893312	38.0	34.0	40.0	25.0	41.0
44	34.96187304877002	38.0	34.0	40.0	25.0	41.0
45	34.93729002620341	38.0	34.0	40.0	25.0	41.0
46	34.80740988865201	38.0	34.0	40.0	24.0	41.0
47	34.622185773594616	37.0	33.0	40.0	24.0	41.0
48	34.515817750995005	37.0	33.0	40.0	23.0	41.0
49	34.455343647488235	37.0	33.0	40.0	24.0	41.0
50	34.28562211646987	37.0	33.0	40.0	23.0	41.0
51	32.38180744124034	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	56.0
10	53.0
11	49.0
12	18.0
13	18.0
14	40.0
15	56.0
16	91.0
17	188.0
18	396.0
19	719.0
20	1348.0
21	1925.0
22	2745.0
23	3608.0
24	4984.0
25	6624.0
26	8158.0
27	9380.0
28	10389.0
29	11925.0
30	14650.0
31	18843.0
32	25121.0
33	34628.0
34	50570.0
35	54336.0
36	61361.0
37	86674.0
38	110162.0
39	86887.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.83805368728838	18.167312401407194	21.83380965895965	12.160824252344776
2	31.34657144554377	30.99823770518854	22.046836119783244	15.608354729484446
3	29.288250707888086	29.438078768637755	26.241361785264044	15.032308738210116
4	26.921363369349272	21.8511355910948	33.96938755305035	17.258113486505575
5	29.684602031589304	26.5910155966391	24.373131274462565	19.351251097309035
6	24.308282785613866	37.18491554845651	25.352458962292175	13.154342703637456
7	63.34806312579617	16.828265360676404	16.152884025160557	3.6707874883668743
8	72.27702350386451	11.085296388945725	11.859353033193186	4.778327073996581
9	66.67860230880422	6.446731834172678	12.91804999108952	13.956615865933585
10	41.827440316289014	21.98792795052374	20.315892995043132	15.868738738144112
11	31.917667170493775	22.497475364174594	26.759984687176168	18.824872778155466
12	27.42035021484156	20.505488195264906	30.84939969770374	21.2247618921898
13	23.99905614922083	22.117294910466185	35.71237632584633	18.17127261446666
14	20.76818232820926	27.77841947896797	29.991683552575125	21.461714640247646
15	18.080352722976496	23.895430574164887	38.97509686021108	19.049119842647535
16	21.8069132119308	24.367520972628327	29.459199904954886	24.366365910485985
17	22.09617377414905	23.711115658022404	31.655138046426895	22.537572521401653
18	23.004382635785806	23.852858283775667	30.97975671091105	22.163002369527483
19	22.304910004158224	26.004079019451247	27.75267809408146	23.93833288230907
20	24.234028790748944	25.931640122238576	31.46603787283756	18.368293214174923
21	23.381097903067186	28.86846812358505	29.942180889331848	17.80825308401592
22	21.47805051911793	21.69454216636855	31.82823235890091	24.999174955612613
23	21.21981162586547	27.651197634432734	31.08981763218861	20.039173107513182
24	23.795435194413457	23.193317800497667	29.130172203264536	23.881074801824337
25	20.895239163867014	30.305035410905106	27.60582019312639	21.193905232101486
26	20.281901166282744	23.766723649732356	30.894777139010078	25.05659804497482
27	22.347152276792492	26.246972087098285	28.47987221712528	22.926003418983942
28	19.340690529150468	24.88828898994766	32.96266839155946	22.808352089342407
29	22.535262397116966	22.95735510570469	32.5268799461411	21.980502551037244
30	21.292250523078142	25.952266231923275	33.82318968760519	18.932293557393386
31	26.078663032071127	23.10074782023273	27.77874949672292	23.041839650973223
32	25.26714937263625	26.508181140145336	29.826014639587612	18.3986548476308
33	23.479773211798793	25.238272819077668	26.77879569920862	24.50315826991492
34	22.79020111281987	23.730256687809803	30.72960325265499	22.749938946715336
35	20.664391744275843	27.070036367956597	29.86611179681467	22.399460090952893
36	26.843314170302364	25.56796055627793	28.11107737596283	19.477647897456883
37	20.762737035252496	25.855736038598877	33.47172077857788	19.909806147570738
38	24.430059337192343	28.19770703663857	25.362524503818307	22.00970912235078
39	23.626136086121434	23.990145669837037	28.83084609952015	23.552872144521373
40	22.02769508999584	28.038143452117726	28.996680021385153	20.937481436501283
41	22.598790814945843	25.410047060531856	26.84611932121948	25.145042803302818
42	21.20826100444204	26.116285056136018	31.45976753549341	21.215686403928533
43	25.4569095817355	23.411294527645587	27.250061053284668	23.88173483733425
44	22.63690786564317	23.28143254107071	30.040361171431023	24.041298421855096
45	20.961407723735537	22.82122278178566	29.124891919185252	27.09247757529355
46	25.51169252905806	25.443543862659812	27.396258918729828	21.648504689552297
47	19.218913977571994	24.462896103810387	34.63453833816259	21.68365158045503
48	22.38576435412225	25.833129822384443	28.064379863636663	23.716725959856642
49	21.448678938926914	22.67419987195311	32.9240563142297	22.95306487489027
50	21.896513032401145	23.61937072214485	30.387209831888956	24.09690641356505
51	21.235487469225845	23.606995056334032	28.28747186598639	26.870045608453736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	601.0
2	992.0
3	4138.5
4	7285.0
5	4901.5
6	2518.0
7	2315.0
8	2112.0
9	2092.5
10	2073.0
11	2060.5
12	2048.0
13	1968.0
14	1888.0
15	1801.5
16	1715.0
17	1687.5
18	1660.0
19	1687.0
20	1714.0
21	1951.5
22	2189.0
23	2618.5
24	3048.0
25	3505.5
26	4259.5
27	4556.0
28	5653.0
29	6750.0
30	8251.5
31	9753.0
32	10772.5
33	11792.0
34	13504.5
35	15217.0
36	17760.0
37	20303.0
38	21077.0
39	21851.0
40	24949.5
41	28048.0
42	32246.5
43	36445.0
44	62158.5
45	87872.0
46	67706.5
47	47541.0
48	47714.5
49	47888.0
50	42530.0
51	37172.0
52	34491.0
53	31810.0
54	30331.0
55	28852.0
56	28305.5
57	27759.0
58	25992.5
59	24226.0
60	22967.5
61	21709.0
62	19966.5
63	18224.0
64	15814.0
65	13404.0
66	11636.0
67	9868.0
68	8745.0
69	7622.0
70	6305.0
71	4988.0
72	4370.0
73	3752.0
74	3075.0
75	1836.0
76	1274.0
77	1006.0
78	738.0
79	573.5
80	409.0
81	291.0
82	173.0
83	138.5
84	104.0
85	66.0
86	28.0
87	34.0
88	40.0
89	30.5
90	21.0
91	16.0
92	11.0
93	9.5
94	8.0
95	6.5
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	606028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.40650445850075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45327152156729	21.89410460948935
2	8.64545443776301	5.08465188939686
3	3.4181533829715116	3.0154782808857346
4	2.1197220806480956	2.4933446726144286
5	1.482543326631223	2.1798208472250797
6	1.0152948291266162	1.7913763151642752
7	0.8387013716377576	1.7264292937091488
8	0.6506795215065886	1.5307368200190903
9	0.5402384583748691	1.429787217136874
>10	6.53749388981031	43.61338753974393
>50	0.25973880511794956	4.547503083744312
>100	0.029732519808317995	1.5276020661493175
>500	0.0022439637591183392	0.4381545451071909
>1k	0.005048918458016263	3.0246850845407924
>5k	0.0	0.0
>10k+	0.0016829728193387544	5.7029377350736405
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	12470	2.0576607021457756	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	11637	1.9202083072069276	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	10463	1.7264878850482155	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3938	0.6498049595068215	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	2910	0.48017583345984016	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	2345	0.3869458176849915	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	2182	0.3600493706561413	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC	1962	0.32374741761106746	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT	1545	0.25493871570290483	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA	1365	0.22523711775693533	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	1047	0.17276429471905588	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	1041	0.17177424145419024	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTT	836	0.13794742157128054	No Hit
GAATCTATCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	751	0.12392166698568383	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09917033536404259	0.0
2	0.0	0.0	0.0	0.8276845294276832	0.0
3	0.0	0.0	0.0	0.9902182737431274	0.0
4	0.0	0.0	0.0	1.5756697710336816	0.0
5	0.0	0.0	0.0	4.015821051172553	0.0
6	0.0	0.0	0.0	4.620248569373032	0.0
7	0.0	0.0	0.0	5.326816582732151	0.0
8	0.0	0.0	0.0	6.1455906327760434	0.0
9	0.0	0.0	0.0	6.33139062881583	0.0
10	0.0	0.0	0.0	9.081263571980173	0.0
11	0.0	0.0	0.0	9.67892572620407	0.0
12	0.0	0.0	0.0	12.393651778465681	0.0
13	0.0	0.0	0.0	12.754196175754256	0.0
14	0.0	0.0	0.0	12.950061713320176	0.0
15	0.0	0.0	0.0	13.380899892414211	0.0
16	0.0	0.0	0.0	13.795237183760486	0.0
17	0.0	0.0	0.0	14.189113374299538	0.0
18	0.0	0.0	0.0	14.633482281346737	0.0
19	0.0	0.0	0.0	15.030988667190295	0.0
20	0.0	0.0	0.0	15.32173430930584	0.0
21	0.0	0.0	0.0	15.655217250688088	0.0
22	0.0	0.0	0.0	16.03160250021451	0.0
23	0.0	0.0	0.0	16.369375672411177	0.0
24	0.0	0.0	0.0	16.65946127901681	0.0
25	0.0	0.0	0.0	16.905654524213404	0.0
26	0.0	0.0	0.0	17.16191331093613	0.0
27	0.0	0.0	0.0	17.465034618862497	0.0
28	0.0	0.0	0.0	17.727068716296937	0.0
29	0.0	0.0	0.0	18.01517421637284	0.0
30	0.0	0.0	0.0	18.381823942128086	0.0
31	0.0	0.0	0.0	18.665474202512094	0.0
32	1.650088774776083E-4	0.0	0.0	18.95275465820061	0.0
33	1.650088774776083E-4	0.0	0.0	19.231949678892725	0.0
34	1.650088774776083E-4	0.0	0.0	19.538206155491164	0.0
35	1.650088774776083E-4	0.0	0.0	19.857498333410337	0.0
36	1.650088774776083E-4	0.0	0.0	20.148078966648406	0.0
37	1.650088774776083E-4	0.0	0.0	20.444929937230622	0.0
38	1.650088774776083E-4	0.0	0.0	20.79359369534081	0.0
39	1.650088774776083E-4	0.0	0.0	21.241097771060083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	20	7.030643E-4	45.000004	36
ACCGACA	20	7.030643E-4	45.000004	16
TTCGTTC	20	7.030643E-4	45.000004	14
ATTCGAA	30	2.1637952E-6	45.000004	13
CGACAAT	30	2.1637952E-6	45.000004	20
GTGCGAT	20	7.030643E-4	45.000004	30
AAGTCGC	20	7.030643E-4	45.000004	22
CGTTCCA	25	3.8886163E-5	44.999996	26
TTACGTA	25	3.8886163E-5	44.999996	44
AACGGCT	100	0.0	42.749996	7
ACGGCTG	1590	0.0	42.45283	8
TACGGCT	1500	0.0	42.15	7
CGTTTTA	145	0.0	41.89655	1
CGTTTTT	1920	0.0	41.601566	1
TTAGACG	55	6.002665E-11	40.909092	1
CATACGA	55	6.002665E-11	40.909092	18
GCGATCA	55	6.002665E-11	40.909092	9
GATACCT	1575	0.0	40.57143	5
CGGCTGT	1665	0.0	40.0	9
TGATACC	1615	0.0	39.566563	4
>>END_MODULE