##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936597.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152456
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9670396704623	33.0	31.0	34.0	30.0	34.0
2	32.0888781025345	33.0	31.0	34.0	30.0	34.0
3	31.9928963110668	33.0	31.0	34.0	30.0	34.0
4	35.74770425565409	37.0	35.0	37.0	35.0	37.0
5	35.81768510258698	37.0	35.0	37.0	35.0	37.0
6	35.777398068951044	37.0	35.0	37.0	35.0	37.0
7	26.813060817547356	35.0	10.0	37.0	0.0	37.0
8	31.12066432282101	35.0	19.0	37.0	17.0	37.0
9	35.896245474104006	39.0	32.0	39.0	32.0	39.0
10	36.63765283098074	38.0	35.0	39.0	33.0	39.0
11	36.9659967466023	39.0	37.0	39.0	33.0	39.0
12	36.95853229784331	39.0	37.0	39.0	33.0	39.0
13	36.841856010914626	39.0	35.0	39.0	33.0	39.0
14	38.00744477095031	40.0	37.0	41.0	33.0	41.0
15	38.11901794616151	40.0	37.0	41.0	33.0	41.0
16	38.073293278060554	40.0	37.0	41.0	33.0	41.0
17	37.975166605446816	40.0	36.0	41.0	33.0	41.0
18	37.87094637141208	40.0	36.0	41.0	33.0	41.0
19	37.75825156110616	40.0	36.0	41.0	33.0	41.0
20	37.82021042136748	40.0	36.0	41.0	33.0	41.0
21	37.790516608070526	40.0	36.0	41.0	33.0	41.0
22	37.7738691819279	40.0	36.0	41.0	33.0	41.0
23	37.70207141732697	39.0	36.0	41.0	33.0	41.0
24	37.655067691661856	39.0	36.0	41.0	33.0	41.0
25	37.57501180668521	39.0	35.0	41.0	33.0	41.0
26	37.39507792412237	39.0	35.0	41.0	33.0	41.0
27	37.278414755732804	39.0	35.0	41.0	32.0	41.0
28	37.24001023246051	39.0	35.0	41.0	32.0	41.0
29	37.235891011176996	39.0	35.0	41.0	32.0	41.0
30	37.140866873065015	39.0	35.0	41.0	32.0	41.0
31	37.031445138269405	39.0	35.0	41.0	31.0	41.0
32	36.87858136117962	39.0	35.0	41.0	31.0	41.0
33	36.80639659967466	39.0	35.0	41.0	31.0	41.0
34	36.65707482814714	39.0	35.0	41.0	31.0	41.0
35	36.55424515925907	39.0	35.0	41.0	30.0	41.0
36	36.39276906123734	39.0	35.0	41.0	30.0	41.0
37	36.28796505221179	39.0	35.0	40.0	30.0	41.0
38	36.182833079708246	39.0	35.0	40.0	30.0	41.0
39	36.11389515663536	39.0	35.0	40.0	30.0	41.0
40	35.906871490790785	38.0	35.0	40.0	29.0	41.0
41	35.79824998688146	38.0	35.0	40.0	28.0	41.0
42	35.751469276381385	38.0	35.0	40.0	28.0	41.0
43	35.707384425670355	38.0	35.0	40.0	28.0	41.0
44	35.505693445977855	38.0	34.0	40.0	27.0	41.0
45	35.38760691609382	38.0	34.0	40.0	27.0	41.0
46	35.26473211943118	38.0	34.0	40.0	26.0	41.0
47	35.10885107834392	38.0	34.0	40.0	26.0	41.0
48	35.039775410610275	38.0	34.0	40.0	26.0	41.0
49	34.82074172220182	37.0	34.0	40.0	24.0	41.0
50	34.63134281366427	37.0	34.0	40.0	24.0	41.0
51	32.39291336516766	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	9.0
10	13.0
11	11.0
12	5.0
13	3.0
14	9.0
15	11.0
16	15.0
17	35.0
18	60.0
19	125.0
20	202.0
21	326.0
22	492.0
23	666.0
24	916.0
25	1275.0
26	1602.0
27	1864.0
28	2196.0
29	2615.0
30	3170.0
31	4042.0
32	5338.0
33	7321.0
34	11467.0
35	14397.0
36	14506.0
37	22402.0
38	27841.0
39	29514.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21341239439576	19.533504748911163	23.625833027234087	12.627249829458991
2	31.300834339088002	24.114498609434854	26.195754840740936	18.388912210736212
3	31.91281418901191	24.82880306449074	27.01697538962061	16.24140735687674
4	28.450831715380176	26.044891640866872	26.928425250564096	18.575851393188856
5	26.015374927848033	30.092616886183553	24.797974497559952	19.09403368840846
6	26.549299470011018	34.437477042556544	25.120034632943277	13.893188854489164
7	61.06089625859264	27.996274334890064	6.847877420370467	4.094951986146823
8	81.93642755942699	5.778716482132549	8.06527785065855	4.219578107781918
9	75.8153172062759	7.689431704885344	9.849399170908328	6.645851917930419
10	44.35115705515034	24.883900928792567	16.8750327963478	13.889909219709292
11	33.45621031641917	25.68675552290497	22.66686781759983	18.190166343076037
12	31.507451330219865	22.578973605499293	27.00254499658918	18.911030067691662
13	25.030828566930786	26.274466075457838	29.026079655769532	19.668625701841844
14	20.298971506533032	29.499002991026916	28.188460932990502	22.013564569449546
15	19.2606391352259	26.635225901243636	34.934013748229	19.170121215301464
16	22.765912787951933	24.548066327333785	31.727186860471217	20.95883402424306
17	22.613737734165923	23.61074670724668	28.468541743191476	25.306973815395917
18	23.437581990869496	26.069160938237918	29.193341029542953	21.299916041349633
19	25.511623025659862	26.79789578632523	25.690035157684843	22.00044603033006
20	26.611612530828566	25.834339087999158	27.21572125728079	20.338327123891485
21	25.695938500288605	26.175421105105734	27.534501757884243	20.594138636721414
22	23.506454321246785	24.611691242063284	27.463661646639032	24.4181927900509
23	23.271632471008026	27.105525528677127	27.390853754525896	22.23198824578895
24	24.05021776774938	24.062680379912894	28.72632103688933	23.160780815448394
25	22.380883664795086	26.72967938290392	27.71947315946896	23.16996379283203
26	20.934564726872015	27.57976071784646	27.539093246576062	23.94658130870546
27	22.209030802329853	28.852259012436377	27.024190586136328	21.914519599097446
28	20.895209109513566	27.02156687831243	29.789578632523483	22.293645379650524
29	22.273311644015322	25.05640971821378	28.56824264049955	24.102035997271344
30	22.40646481607808	25.851393188854487	28.670567245631528	23.071574749435904
31	24.850448654037887	26.24954085113082	25.789736054992918	23.11027443983838
32	24.84126567665425	27.514823949205013	25.336490528414757	22.307419845725978
33	23.81801962533452	26.73295901768379	25.821220548879676	23.62780080810201
34	22.123760298053206	26.33940284409928	27.703074985569607	23.833761872277904
35	21.72233300099701	26.260035682426402	27.567954032638927	24.44967728393766
36	23.2545783701527	27.75948470378339	26.933016739255915	22.052920186808
37	22.72196568190166	27.59550296478984	27.8506585506638	21.831872802644696
38	22.51731647163772	27.978564307078763	25.120690559899252	24.38342866138427
39	23.174555281523848	26.084903185181297	26.78280946633783	23.957732066957025
40	22.719997901033743	24.655638348113555	28.747310699480504	23.8770530513722
41	20.23731437267146	26.609644749960644	28.4908432596946	24.662197617673296
42	22.61504958807787	26.52962166133179	27.259012436375084	23.596316314215247
43	23.269008763184132	26.359080652778506	26.47845935876581	23.89345122527155
44	22.156556645851918	26.222647845935874	26.607021042136747	25.013774466075457
45	21.383218764758357	25.854016896678385	26.363672141470325	26.399092197092934
46	23.133231883297476	27.097654405205436	26.3059505693446	23.463163142152492
47	21.294668625701842	26.12753843731962	29.40520543632261	23.17258750065593
48	21.70265519231778	24.99081702261636	28.673190953455425	24.633336831610432
49	21.1687306501548	24.975730702628958	29.5580364170646	24.29750223015165
50	20.23600251875951	25.850081334942544	28.75714960382012	25.156766542477833
51	20.262239596998477	25.66838956813769	26.904155953193055	27.165214881670778
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	200.5
2	328.0
3	484.5
4	641.0
5	481.0
6	321.0
7	315.5
8	310.0
9	317.0
10	324.0
11	320.5
12	317.0
13	339.5
14	362.0
15	349.0
16	336.0
17	396.0
18	456.0
19	456.5
20	457.0
21	492.5
22	528.0
23	620.5
24	713.0
25	881.0
26	1144.5
27	1240.0
28	1552.5
29	1865.0
30	2227.0
31	2589.0
32	2792.5
33	2996.0
34	3453.0
35	3910.0
36	4273.0
37	4636.0
38	5058.5
39	5481.0
40	6409.5
41	7338.0
42	8214.5
43	9091.0
44	10103.5
45	11116.0
46	12731.0
47	14346.0
48	13654.5
49	12963.0
50	12514.5
51	12066.0
52	10934.0
53	9802.0
54	9077.5
55	8353.0
56	8138.0
57	7923.0
58	7602.0
59	7281.0
60	6981.5
61	6682.0
62	6020.5
63	5359.0
64	4473.0
65	3587.0
66	3033.0
67	2479.0
68	2157.0
69	1835.0
70	1547.5
71	1260.0
72	1017.0
73	774.0
74	619.5
75	425.0
76	385.0
77	306.0
78	227.0
79	160.5
80	94.0
81	65.5
82	37.0
83	28.5
84	20.0
85	20.5
86	21.0
87	16.0
88	11.0
89	8.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152456.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.77226216088576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4321544727358	31.08634622448444
2	9.541738697295555	8.544104528519703
3	5.297547540215066	7.115495618407934
4	3.6508541123384806	6.5382798971506535
5	2.6766093351695037	5.9918927428241595
6	2.083272290427496	5.596368788371727
7	1.6847842011192826	5.280211995592171
8	1.2804359928506548	4.586241276171486
9	1.05042632365437	4.232696646901401
>10	3.2728764393917196	19.03303248150286
>50	0.01758035688124469	0.6041087264522222
>100	0.011720237920829794	1.3912210736212416
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	458	0.30041454583617566	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	441	0.2892637875846146	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	410	0.2689300519494149	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	233	0.15283098074198456	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	221	0.14495985727029437	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12331426772314635	0.0
2	0.0	0.0	0.0	0.5752479403893582	0.0
3	0.0	0.0	0.0	0.753660072414336	0.0
4	0.0	0.0	0.0	1.0140630739360865	0.0
5	0.0	0.0	0.0	1.9907383113816446	0.0
6	0.0	0.0	0.0	2.419058613632786	0.0
7	0.0	0.0	0.0	2.8782074828147137	0.0
8	0.0	0.0	0.0	3.7138584247258226	0.0
9	0.0	0.0	0.0	3.970325864511728	0.0
10	0.0	0.0	0.0	4.977829668888073	0.0
11	0.0	0.0	0.0	5.797082436899827	0.0
12	0.0	0.0	0.0	6.779005089993178	0.0
13	0.0	0.0	0.0	7.116807472319882	0.0
14	0.0	0.0	0.0	7.247992863514719	0.0
15	0.0	0.0	0.0	7.501836595476727	0.0
16	0.0	0.0	0.0	7.9931258855013905	0.0
17	0.0	0.0	0.0	8.557223067639187	0.0
18	0.0	0.0	0.0	9.227580416644802	0.0
19	0.0	0.0	0.0	9.592275804166448	0.0
20	0.0	0.0	0.0	9.97533714645537	0.0
21	0.0	0.0	0.0	10.418087841737943	0.0
22	0.0	0.0	0.0	10.913968620454426	0.0
23	0.0	0.0	0.0	11.38033268615207	0.0
24	0.0	0.0	0.0	11.766673663220864	0.0
25	0.0	0.0	0.0	12.090045652516135	0.0
26	0.0	0.0	0.0	12.372750170541009	0.0
27	0.0	0.0	0.0	12.675132497245107	0.0
28	0.0	0.0	0.0	12.982106312641024	0.0
29	0.0	0.0	0.0	13.314661279319935	0.0
30	0.0	0.0	0.0	13.678700739885606	0.0
31	0.0	0.0	0.0	14.031589442199717	0.0
32	0.0	0.0	0.0	14.396284829721361	0.0
33	0.0	0.0	0.0	14.745893897255602	0.0
34	0.0	0.0	0.0	15.064674397859054	0.0
35	0.0	0.0	0.0	15.445112032324081	0.0
36	0.0	0.0	0.0	15.773075510311171	0.0
37	0.0	0.0	0.0	16.146297948260482	0.0
38	0.0	0.0	0.0	16.514928897517972	0.0
39	0.0	0.0	0.0	16.893398751115075	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	25	3.8748498E-5	45.000004	5
CCACAAT	20	7.014027E-4	45.0	11
GTTGATC	35	1.2026794E-7	45.0	16
AACGTGA	20	7.014027E-4	45.0	18
TTTCGAC	20	7.014027E-4	45.0	24
GACGTAA	20	7.014027E-4	45.0	19
CGCATGG	20	7.014027E-4	45.0	2
TGTATCG	20	7.014027E-4	45.0	4
TGAAACG	20	7.014027E-4	45.0	4
AGACGTA	20	7.014027E-4	45.0	18
TCGTGAA	20	7.014027E-4	45.0	27
CTAGTAG	20	7.014027E-4	45.0	28
TAATTCG	20	7.014027E-4	45.0	23
TACGAAT	20	7.014027E-4	45.0	12
GTCCGCT	20	7.014027E-4	45.0	43
CGAATAT	20	7.014027E-4	45.0	14
AATTCGT	20	7.014027E-4	45.0	24
GTATCGC	20	7.014027E-4	45.0	5
TAGGCAC	20	7.014027E-4	45.0	6
GGTCTAT	20	7.014027E-4	45.0	8
>>END_MODULE