##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936595.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	209549
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862394952970426	31.0	31.0	34.0	30.0	34.0
2	31.989062224109873	33.0	31.0	34.0	30.0	34.0
3	31.821478508606578	33.0	31.0	34.0	30.0	34.0
4	35.6311316207665	37.0	35.0	37.0	33.0	37.0
5	35.739359290667096	37.0	35.0	37.0	35.0	37.0
6	35.73396198502498	37.0	35.0	37.0	35.0	37.0
7	27.065874807324303	35.0	18.0	37.0	0.0	37.0
8	31.3058043703382	36.0	25.0	37.0	17.0	37.0
9	35.99021231311054	39.0	32.0	39.0	32.0	39.0
10	36.522102229072914	37.0	35.0	39.0	32.0	39.0
11	36.93601496547347	39.0	37.0	39.0	33.0	39.0
12	37.10357004805559	39.0	37.0	39.0	34.0	39.0
13	37.14208609919398	39.0	37.0	39.0	34.0	39.0
14	38.3139122591852	40.0	38.0	41.0	34.0	41.0
15	38.37168394981603	40.0	38.0	41.0	34.0	41.0
16	38.28482121126801	40.0	37.0	41.0	34.0	41.0
17	38.20307422130385	40.0	37.0	41.0	34.0	41.0
18	38.10274446549494	40.0	37.0	41.0	33.0	41.0
19	37.99394890932431	40.0	37.0	41.0	33.0	41.0
20	38.04549771175238	40.0	37.0	41.0	34.0	41.0
21	37.99342874458957	40.0	37.0	41.0	33.0	41.0
22	38.03454084724814	40.0	37.0	41.0	34.0	41.0
23	37.988661363213374	40.0	37.0	41.0	33.0	41.0
24	37.94718180473302	40.0	37.0	41.0	33.0	41.0
25	37.80918066896048	40.0	36.0	41.0	33.0	41.0
26	37.65128919727606	39.0	36.0	41.0	33.0	41.0
27	37.54843974440346	39.0	36.0	41.0	33.0	41.0
28	37.45366477530315	39.0	36.0	41.0	32.0	41.0
29	37.351769753136495	39.0	36.0	41.0	32.0	41.0
30	37.284744856811535	39.0	36.0	41.0	32.0	41.0
31	37.11611603968523	39.0	36.0	41.0	31.0	41.0
32	36.938625333454226	39.0	35.0	41.0	31.0	41.0
33	36.92523944280335	39.0	35.0	41.0	31.0	41.0
34	36.70787739383152	39.0	35.0	41.0	30.0	41.0
35	36.655956363428125	39.0	35.0	41.0	30.0	41.0
36	36.463915361085	39.0	35.0	41.0	30.0	41.0
37	36.439357859021044	39.0	35.0	41.0	30.0	41.0
38	36.36079866761474	39.0	35.0	40.0	30.0	41.0
39	36.38595746102344	39.0	35.0	40.0	30.0	41.0
40	36.19692291540404	39.0	35.0	40.0	29.0	41.0
41	36.11279462082854	39.0	35.0	40.0	29.0	41.0
42	35.97998081594281	39.0	35.0	40.0	28.0	41.0
43	35.92196097332843	39.0	35.0	40.0	28.0	41.0
44	35.71209597755179	39.0	35.0	40.0	27.0	41.0
45	35.63315024170958	38.0	35.0	40.0	27.0	41.0
46	35.43669976950498	38.0	34.0	40.0	26.0	41.0
47	35.262668874583035	38.0	34.0	40.0	25.0	41.0
48	35.21572520031114	38.0	34.0	40.0	24.0	41.0
49	35.03212136540857	38.0	34.0	40.0	24.0	41.0
50	34.7679063130819	38.0	34.0	40.0	23.0	41.0
51	32.4559840419186	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	11.0
10	16.0
11	4.0
12	2.0
13	4.0
14	8.0
15	9.0
16	8.0
17	25.0
18	41.0
19	113.0
20	204.0
21	301.0
22	542.0
23	847.0
24	1253.0
25	1646.0
26	2264.0
27	2617.0
28	2977.0
29	3504.0
30	4432.0
31	5468.0
32	6984.0
33	9413.0
34	13622.0
35	17600.0
36	20637.0
37	31869.0
38	41307.0
39	41807.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44423499992842	21.11630215367289	25.576834057905312	13.862628788493383
2	30.691628211062806	25.06907692234275	27.30053591284139	16.938758953753062
3	30.029730516490176	24.82235658485605	28.571360397806718	16.576552500847058
4	26.333697607719436	25.905635436103253	29.296250518971696	18.464416437205617
5	23.987706932507432	30.15905587714568	27.25567766966199	18.5975595206849
6	24.94786422268777	34.42249784060053	26.6224128962677	14.007225040444002
7	62.115781988938146	26.677769877212487	7.159184725290982	4.04726340855838
8	83.36140950326654	3.8077013013662677	8.280163589423	4.550725605944194
9	77.46398217123442	6.575550348605816	10.391364310972612	5.569103169187159
10	35.53631847443796	30.374757216689176	19.223188848431633	14.865735460441234
11	25.070031353048687	27.320101742313252	28.596175596161277	19.013691308476776
12	22.886771113200254	24.303146280822148	32.28027812110771	20.52980448486989
13	21.549613694171768	26.754601549041034	33.99825339180812	17.697531364979074
14	19.255162277080778	29.502407551455747	30.81474977212967	20.42768039933381
15	17.458446473139933	28.42342363838529	35.7839932426306	18.334136645844172
16	20.747414685825273	27.559186634152393	31.839808350314247	19.853590329708087
17	21.054264157786484	26.836682589752275	29.451822724040678	22.65723052842056
18	21.196951548325213	26.737899011687006	32.33134016387575	19.73380927611203
19	20.90823625977695	28.738385771347037	28.89538962247493	21.45798834640108
20	21.558203570525272	28.075056430715488	30.610024385704538	19.756715613054705
21	20.556528544636336	29.017556752835855	31.350185398164633	19.07572930436318
22	20.000572658423565	26.013963321227973	30.175281199146735	23.81018282120172
23	18.693002591279367	28.54988570692296	30.06361280655121	22.69349889524646
24	19.381624345618448	26.299338102305427	31.81928809013644	22.49974946193969
25	18.137523920419568	29.92808364630707	29.78969119394509	22.14470123932827
26	18.246806236250233	30.082224205317132	30.12851409455545	21.542455463877182
27	19.802051071587076	28.491665433860337	29.971987458780525	21.734296035772065
28	17.474671795140992	27.839312046347153	31.982972956205945	22.703043202305906
29	18.443418961674833	27.050475067883884	31.548706985001125	22.957398985440157
30	18.804670983874892	27.12301180153568	32.11372996292037	21.95858725166906
31	18.526931648444993	27.648903120511193	29.01039852254127	24.813766708502545
32	18.904886207999084	28.09987162907005	30.143784985850562	22.8514571770803
33	18.170928995127632	25.714272079561347	31.678032345656625	24.4367665796544
34	16.811342454509447	26.521243241437563	31.035223265202887	25.6321910388501
35	16.60995757555512	26.106543099704606	31.944795727968163	25.338703596772117
36	18.54267975509308	26.531264763849983	30.370462278512424	24.55559320254451
37	17.129167879589023	26.26259252012656	32.019718538384815	24.588521061899606
38	16.985048842991375	26.820934483104192	30.766551021479465	25.427465652424967
39	18.270189788545878	25.09389212069731	31.393611995285113	25.242306095471704
40	19.173081236369534	25.156884547289653	30.968890331139733	24.701143885201073
41	17.221747658065656	26.263069735479526	29.31533913309059	27.199843473364226
42	18.87577607146777	25.273325093414904	30.43154584369288	25.41935299142444
43	19.320540780437987	25.003221203632563	30.889195367193352	24.787042648736097
44	18.543156970446052	25.671322697793837	30.383824308395653	25.40169602336446
45	18.251101174426985	25.134455425699954	30.947415640255976	25.667027759617085
46	20.084085345193724	25.99916964528583	29.759149411354862	24.157595598165585
47	17.378274293840583	25.413626407188772	31.772998200898115	25.435101098072526
48	18.21149230013028	24.603314737841746	31.093443538265515	26.09174942376246
49	19.34726484020444	22.673933065774595	32.51459085941713	25.46421123460384
50	18.125593536595257	23.945234766092895	32.25546292275315	25.673708774558694
51	17.975270700408974	23.192188939102547	29.6894759698209	29.14306439066758
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	139.0
2	224.0
3	817.5
4	1411.0
5	1019.5
6	628.0
7	567.5
8	507.0
9	518.5
10	530.0
11	495.0
12	460.0
13	479.5
14	499.0
15	475.5
16	452.0
17	491.0
18	530.0
19	572.5
20	615.0
21	727.5
22	840.0
23	965.5
24	1091.0
25	1363.5
26	2001.0
27	2366.0
28	2919.5
29	3473.0
30	3906.5
31	4340.0
32	4930.5
33	5521.0
34	6484.0
35	7447.0
36	8025.0
37	8603.0
38	9572.0
39	10541.0
40	11615.5
41	12690.0
42	14144.5
43	15599.0
44	16932.5
45	18266.0
46	20780.0
47	23294.0
48	23482.5
49	23671.0
50	22202.0
51	20733.0
52	17333.5
53	13934.0
54	11565.0
55	9196.0
56	7631.5
57	6067.0
58	5398.0
59	4729.0
60	4149.5
61	3570.0
62	2985.5
63	2401.0
64	1910.0
65	1419.0
66	1159.5
67	900.0
68	723.0
69	546.0
70	430.5
71	315.0
72	242.5
73	170.0
74	146.0
75	95.5
76	69.0
77	49.0
78	29.0
79	28.5
80	28.0
81	20.5
82	13.0
83	9.5
84	6.0
85	5.5
86	5.0
87	3.5
88	2.0
89	1.5
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	209549.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.861134407334774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54669734715038	37.82039584868618
2	11.876913254052083	12.55654215733406
3	5.396651560161081	8.558193704136835
4	3.286886519774808	6.949942004138918
5	2.2194214272797357	5.866056718697645
6	1.5983655867806696	5.069485086892289
7	1.1400400826984223	4.218466842888924
8	0.8358830827225185	3.534858238768991
9	0.607280327515525	2.8891374314155605
>10	1.456080008857252	10.001376640017499
>50	0.025841691060997565	0.9131325690771216
>100	0.008945200751883773	1.2677726635115616
>500	9.939111946537526E-4	0.35464009443444017
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	675	0.32212036325632665	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTC	438	0.20902032460188308	No Hit
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	365	0.17418360383490258	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT	347	0.16559372748140053	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC	334	0.15938992789276016	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCG	267	0.12741649924361367	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGC	241	0.11500890006633294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24815198354561463	0.0
2	0.0	0.0	0.0	0.9782914735932885	0.0
3	0.0	0.0	0.0	1.3653131248538528	0.0
4	0.0	0.0	0.0	1.8248715097662123	0.0
5	0.0	0.0	0.0	3.2374289545643262	0.0
6	0.0	0.0	0.0	4.1331621720934	0.0
7	0.0	0.0	0.0	4.926294088733423	0.0
8	0.0	0.0	0.0	6.604660485137128	0.0
9	0.0	0.0	0.0	7.208337906647133	0.0
10	0.0	0.0	0.0	8.765491603395864	0.0
11	0.0	0.0	0.0	10.458651675741711	0.0
12	0.0	0.0	0.0	11.89459267283547	0.0
13	0.0	0.0	0.0	12.514972631699507	0.0
14	0.0	0.0	0.0	12.788417028952656	0.0
15	0.0	0.0	0.0	13.209320970274256	0.0
16	0.0	0.0	0.0	14.05590100644718	0.0
17	0.0	0.0	0.0	15.192627977227284	0.0
18	0.0	0.0	0.0	16.344625839302502	0.0
19	0.0	0.0	0.0	17.03277037828861	0.0
20	0.0	0.0	0.0	17.722346563333634	0.0
21	0.0	0.0	0.0	18.577993691213035	0.0
22	0.0	0.0	0.0	19.46800032450644	0.0
23	0.0	0.0	0.0	20.396661401390606	0.0
24	0.0	0.0	0.0	21.104848985201553	0.0
25	0.0	0.0	0.0	21.7233200826537	0.0
26	0.0	0.0	0.0	22.294546860161585	0.0
27	0.0	0.0	0.0	22.9101546654959	0.0
28	0.0	0.0	0.0	23.54771437706694	0.0
29	0.0	0.0	0.0	24.170003197342865	0.0
30	0.0	0.0	0.0	24.911118640508903	0.0
31	0.0	0.0	0.0	25.590673303141507	0.0
32	0.0	0.0	0.0	26.23061909147741	0.0
33	0.0	0.0	0.0	26.84909018892956	0.0
34	0.0	0.0	0.0	27.416499243613664	0.0
35	0.0	0.0	0.0	28.049764017007956	0.0
36	0.0	0.0	0.0	28.597607242220196	0.0
37	0.0	0.0	0.0	29.218464416437207	0.0
38	0.0	0.0	0.0	29.7877823325332	0.0
39	0.0	0.0	0.0	30.402435707161573	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	20	7.02007E-4	45.000004	2
CGGTTTT	30	2.1569795E-6	45.000004	1
CGCATGG	40	6.7684596E-9	45.000004	2
CGGTTAA	20	7.02007E-4	45.000004	35
TACGGTT	20	7.02007E-4	45.000004	33
CGATTGA	40	6.7684596E-9	45.000004	10
TACGGGT	20	7.02007E-4	45.000004	4
TACGCGG	20	7.02007E-4	45.000004	2
ACAGCGG	30	2.1569795E-6	45.000004	2
ACGGTTA	20	7.02007E-4	45.000004	34
CGGGTCT	25	3.8798542E-5	45.0	6
GCCCTAC	25	3.8798542E-5	45.0	26
GGTATGA	25	3.8798542E-5	45.0	8
AGCTGCG	25	3.8798542E-5	45.0	1
TGCACGG	25	3.8798542E-5	45.0	2
GGCGATT	120	0.0	43.125004	8
GGCGATA	60	3.6379788E-12	41.250004	8
CGTTTTT	340	0.0	41.02941	1
CAGTCTC	100	0.0	40.5	6
GCGACTG	45	1.9153958E-8	40.0	20
>>END_MODULE