##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936593.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311236
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.944116361860452	33.0	31.0	34.0	30.0	34.0
2	32.060336850492874	33.0	31.0	34.0	30.0	34.0
3	31.699202534411185	33.0	31.0	34.0	30.0	34.0
4	35.590224138595794	37.0	35.0	37.0	33.0	37.0
5	35.75569664177666	37.0	35.0	37.0	35.0	37.0
6	35.75899638859258	37.0	35.0	37.0	35.0	37.0
7	27.123716408127596	35.0	19.0	37.0	0.0	37.0
8	31.29330797208549	35.0	25.0	37.0	17.0	37.0
9	36.013002994512206	39.0	32.0	39.0	32.0	39.0
10	36.73998187870298	38.0	35.0	39.0	33.0	39.0
11	37.062132915215464	39.0	37.0	39.0	34.0	39.0
12	37.123742112095	39.0	37.0	39.0	34.0	39.0
13	37.08743204513617	39.0	37.0	39.0	33.0	39.0
14	38.25828952948888	40.0	38.0	41.0	34.0	41.0
15	38.309022735159175	40.0	38.0	41.0	33.0	41.0
16	38.27109331825367	40.0	37.0	41.0	33.0	41.0
17	38.15320207173977	40.0	37.0	41.0	33.0	41.0
18	38.001571155007774	40.0	37.0	41.0	33.0	41.0
19	37.81995977329101	40.0	37.0	41.0	33.0	41.0
20	37.84371345217134	40.0	36.0	41.0	33.0	41.0
21	37.78501522960069	40.0	36.0	41.0	33.0	41.0
22	37.809736020254725	40.0	36.0	41.0	33.0	41.0
23	37.75773368119369	39.0	36.0	41.0	33.0	41.0
24	37.67354997493863	39.0	35.0	41.0	33.0	41.0
25	37.572481975092856	39.0	35.0	41.0	33.0	41.0
26	37.4527368299297	39.0	35.0	41.0	33.0	41.0
27	37.311400352144354	39.0	35.0	41.0	33.0	41.0
28	37.27203151306404	39.0	35.0	41.0	33.0	41.0
29	37.16467246719531	39.0	35.0	41.0	32.0	41.0
30	36.97109267565449	39.0	35.0	41.0	31.0	41.0
31	36.821646596152114	39.0	35.0	41.0	31.0	41.0
32	36.50169003585703	39.0	35.0	41.0	30.0	41.0
33	36.344474932205785	39.0	35.0	41.0	30.0	41.0
34	36.06320284285879	39.0	35.0	41.0	29.0	41.0
35	35.5666375355036	39.0	35.0	41.0	25.0	41.0
36	35.414659615211605	38.0	35.0	40.0	25.0	41.0
37	35.226275238083	38.0	34.0	40.0	24.0	41.0
38	35.00496086570962	38.0	34.0	40.0	23.0	41.0
39	34.91671271960827	38.0	34.0	40.0	23.0	41.0
40	34.58647135935431	38.0	34.0	40.0	21.0	41.0
41	34.459583724247835	38.0	33.0	40.0	21.0	41.0
42	34.32810471796322	38.0	33.0	40.0	21.0	41.0
43	34.24177794342557	38.0	33.0	40.0	20.0	41.0
44	34.09247002274801	38.0	33.0	40.0	20.0	41.0
45	33.95226130653266	38.0	33.0	40.0	20.0	41.0
46	33.74689945893149	37.0	33.0	40.0	18.0	41.0
47	33.598124895577634	37.0	33.0	40.0	18.0	41.0
48	33.44709480908378	37.0	32.0	40.0	17.0	41.0
49	33.289789741546606	37.0	32.0	40.0	15.0	41.0
50	33.12593337531648	36.0	32.0	40.0	15.0	41.0
51	31.00403552288296	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	11.0
10	12.0
11	16.0
12	8.0
13	13.0
14	9.0
15	24.0
16	50.0
17	84.0
18	132.0
19	272.0
20	513.0
21	807.0
22	1316.0
23	2161.0
24	3231.0
25	4581.0
26	5707.0
27	6154.0
28	6588.0
29	6877.0
30	7598.0
31	8826.0
32	10558.0
33	14133.0
34	21147.0
35	24938.0
36	28604.0
37	44514.0
38	54708.0
39	57626.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68625737382565	18.666221131231605	22.52824223418885	16.119279260753895
2	35.02101299335553	24.866981968666863	23.766530864038867	16.345474173938747
3	30.7181688493619	24.73557043529669	29.312483131771387	15.233777583570024
4	26.682645966404916	24.448328599519336	30.63912914958424	18.229896284491513
5	25.693364520813788	28.250587978254444	27.782133172255136	18.273914328676632
6	25.561631687851023	34.08378208176432	27.00041126347852	13.354174966906143
7	59.35849323343058	26.612281355627243	10.124792761762778	3.9044326491794004
8	79.52871775758588	5.582580421288026	11.264763716279608	3.623938104846483
9	73.25887750774332	6.938143402434166	12.810857355832873	6.992121733989641
10	38.98263697001632	27.32395995321878	19.631083807785732	14.062319268979168
11	29.908493876029763	24.619902581963526	27.877237851662407	17.594365690344304
12	27.09937153799689	22.214653831818943	31.157706692027915	19.528267938156255
13	23.329242118520995	23.697772751224154	33.68344278939454	19.28954234086031
14	21.18039044326492	25.612718323073164	32.553110822655476	20.65378041100644
15	20.331516919636545	23.899548895372	35.90330167461347	19.865632510377974
16	24.295068693852897	25.2364765001478	30.866287961546863	19.60216684445244
17	24.043169813260675	24.259404439075173	31.010551478620723	20.686874269043425
18	24.926743692888998	23.585960493002094	31.506638049582953	19.980657764525954
19	24.30181598529733	25.920523332776412	28.956161883586734	20.821498798339526
20	25.375599223740185	26.160534128442727	30.019663535066638	18.444203112750454
21	24.387602976519425	26.576938400442106	30.91126990450976	18.124188718528707
22	23.906617486409026	23.093729517150972	30.94757675847267	22.05207623796733
23	22.435707951522318	25.872649693480188	31.25698826613888	20.434654088858615
24	22.890346875040162	24.111927926075392	31.4314539449164	21.56627125396805
25	22.761505738410722	27.238494261589274	28.839530131475794	21.160469868524206
26	22.20533614363377	25.130126334999808	29.849053451400227	22.8154840699662
27	22.054646634708067	25.50604685833258	30.169389145214566	22.269917361744785
28	20.247979025562596	25.930804919739366	31.543587502731047	22.277628551966995
29	24.225346682260408	24.64335745222275	29.562775514400645	21.568520351116195
30	23.729902710483366	23.960917117557095	32.10907478569317	20.20010538626637
31	24.950841162333408	24.0952203472606	30.260959529103314	20.69297896130268
32	26.460306648331173	24.581346630852472	29.272641982289965	19.68570473852639
33	24.76609389659294	24.02228533974219	29.52261306532663	21.68900769833824
34	25.053978331555477	24.317880964926935	31.119793340102042	19.50834736341554
35	23.61552005552057	25.188924160444166	31.276266241694405	19.91928954234086
36	24.82681951959285	26.144469148813123	30.10480792710355	18.92390340449048
37	24.077548869668032	25.8845377784061	30.104165327918363	19.933748024007507
38	23.832397280520247	26.197162281998228	29.616753845956122	20.353686591525403
39	25.191815856777495	24.39949106144533	28.955197984808954	21.45349509696822
40	26.0802092302947	23.670783585446415	29.970183397807453	20.27882378645144
41	22.24646249148556	25.029238262925883	30.258710431955173	22.46558881363338
42	23.059671760336208	24.297639090593634	31.323175982212852	21.319513166857305
43	23.138390160521276	24.37603619118611	30.08810034828876	22.397473300003856
44	23.46065365189117	24.12992070326055	29.58783688262283	22.82158876222545
45	22.973884769114115	23.93424925137195	29.16179362284569	23.93007235666825
46	23.85360305363133	25.003534295518513	28.396136693698672	22.746725957151487
47	21.79085966918994	24.813967535889166	31.86777879165649	21.527394003264405
48	22.900628462003112	24.5109820200748	30.139829582696088	22.448559935226005
49	22.90223495996607	23.878664421853514	30.976172422213367	22.24292819596705
50	21.82845172152322	24.490418846148902	30.903237414694956	22.777892017632922
51	22.204372244855993	23.68106517240936	29.64920510480793	24.46535747792672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	130.0
1	305.0
2	480.0
3	3119.0
4	5758.0
5	3927.0
6	2096.0
7	1814.5
8	1533.0
9	1508.5
10	1484.0
11	1436.5
12	1389.0
13	1368.5
14	1348.0
15	1280.5
16	1213.0
17	1236.5
18	1260.0
19	1232.5
20	1205.0
21	1289.5
22	1374.0
23	1494.0
24	1614.0
25	1839.0
26	2440.0
27	2816.0
28	3337.5
29	3859.0
30	4440.0
31	5021.0
32	5863.5
33	6706.0
34	7753.5
35	8801.0
36	9396.5
37	9992.0
38	10807.0
39	11622.0
40	12759.0
41	13896.0
42	15094.5
43	16293.0
44	17828.5
45	19364.0
46	22495.0
47	25626.0
48	25587.5
49	25549.0
50	24836.0
51	24123.0
52	22897.0
53	21671.0
54	19994.5
55	18318.0
56	17053.5
57	15789.0
58	14767.5
59	13746.0
60	12717.5
61	11689.0
62	10799.0
63	9909.0
64	8768.5
65	7628.0
66	6447.0
67	5266.0
68	4435.5
69	3605.0
70	3052.0
71	2499.0
72	2112.0
73	1725.0
74	1463.5
75	956.0
76	710.0
77	557.0
78	404.0
79	301.0
80	198.0
81	155.5
82	113.0
83	87.5
84	62.0
85	54.0
86	46.0
87	31.5
88	17.0
89	14.5
90	12.0
91	9.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	311236.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.737030217973654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.52524203070773	36.28978366259898
2	10.54434423625611	10.699774242872854
3	5.522280311014083	8.405523090361276
4	3.486730146845013	7.076253312895806
5	2.448996068443094	6.212739376414798
6	1.7564384201103647	5.346988151828969
7	1.2857832771979005	4.566577749226855
8	1.0094649632640422	4.097380347609071
9	0.72883167754065	3.328087936247675
>10	1.6641806642288335	10.665838254348662
>50	0.0119649064420925	0.41344241194827963
>100	0.01259463836009737	1.0511789299148686
>500	0.0025189276720194737	0.9029275549967839
>1k	6.297319180048684E-4	0.9435049787351389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2953	0.9487976969245203	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCG	929	0.29848732151807633	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGCCGTCTTC	753	0.24193859322186378	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTGGGTTTCGTATGC	618	0.19856314822192805	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.16900358570345334	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.212995925921166E-4	0.0	0.0	0.08546569162950302	0.0
2	3.212995925921166E-4	0.0	0.0	0.45463892351784496	0.0
3	3.212995925921166E-4	0.0	0.0	0.633602796591654	0.0
4	3.212995925921166E-4	0.0	0.0	0.903815753961624	0.0
5	3.212995925921166E-4	0.0	0.0	1.9473968307008187	0.0
6	3.212995925921166E-4	0.0	0.0	2.5138480124407203	0.0
7	3.212995925921166E-4	0.0	0.0	3.0606999190325026	0.0
8	3.212995925921166E-4	0.0	0.0	3.8321402408461744	0.0
9	3.212995925921166E-4	0.0	0.0	4.2671798892159005	0.0
10	3.212995925921166E-4	0.0	0.0	5.604750093176881	0.0
11	3.212995925921166E-4	0.0	0.0	6.378760811731291	0.0
12	3.212995925921166E-4	0.0	0.0	7.488529604544461	0.0
13	3.212995925921166E-4	0.0	0.0	7.799868909766222	0.0
14	3.212995925921166E-4	0.0	0.0	7.976262386099294	0.0
15	3.212995925921166E-4	0.0	0.0	8.210168489506355	0.0
16	3.212995925921166E-4	0.0	0.0	8.578056523024328	0.0
17	3.212995925921166E-4	0.0	0.0	8.97004202598671	0.0
18	3.212995925921166E-4	0.0	0.0	9.414078062949017	0.0
19	3.212995925921166E-4	0.0	0.0	9.69232351013379	0.0
20	3.212995925921166E-4	0.0	0.0	9.97185415568893	0.0
21	3.212995925921166E-4	0.0	0.0	10.30889742831806	0.0
22	3.212995925921166E-4	0.0	0.0	10.665861275687902	0.0
23	3.212995925921166E-4	0.0	0.0	11.031178912465139	0.0
24	3.212995925921166E-4	0.0	0.0	11.337698723798018	0.0
25	3.212995925921166E-4	0.0	0.0	11.59441709827912	0.0
26	3.212995925921166E-4	0.0	0.0	11.851135472760221	0.0
27	3.212995925921166E-4	0.0	0.0	12.135806911796836	0.0
28	3.212995925921166E-4	0.0	0.0	12.413409759796425	0.0
29	3.212995925921166E-4	0.0	0.0	12.67848192368492	0.0
30	3.212995925921166E-4	0.0	0.0	13.013276099165907	0.0
31	3.212995925921166E-4	0.0	0.0	13.298911436980298	0.0
32	3.212995925921166E-4	0.0	0.0	13.618283232016863	0.0
33	3.212995925921166E-4	0.0	0.0	13.916770553534938	0.0
34	3.212995925921166E-4	0.0	0.0	14.20690408564562	0.0
35	3.212995925921166E-4	0.0	0.0	14.522098985978486	0.0
36	3.212995925921166E-4	0.0	0.0	14.82187150586693	0.0
37	3.212995925921166E-4	0.0	0.0	15.135459908236836	0.0
38	3.212995925921166E-4	0.0	0.0	15.487925561310389	0.0
39	3.212995925921166E-4	0.0	0.0	15.961521160791168	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCGC	20	7.025349E-4	45.000004	21
GTAGCCG	20	7.025349E-4	45.000004	43
CTCGCGC	20	7.025349E-4	45.000004	33
CGCGTGA	20	7.025349E-4	45.000004	41
TCGACTC	20	7.025349E-4	45.000004	27
TCGGGCA	25	3.8842285E-5	45.0	5
CGCAATC	45	3.8198777E-10	45.0	20
ATTTGCG	25	3.8842285E-5	45.0	12
CGTTTTA	205	0.0	42.80488	1
CGTTTTT	1265	0.0	41.086956	1
GCGCAAT	50	1.0768417E-9	40.5	19
AACGGCT	45	1.920489E-8	40.0	7
TTAATCG	40	3.447385E-7	39.375004	20
CGTTGAT	40	3.447385E-7	39.375004	25
CGAATAT	35	6.2336167E-6	38.571426	14
AGCTACG	35	6.2336167E-6	38.571426	9
TGCACGG	35	6.2336167E-6	38.571426	2
TACGGCT	125	0.0	37.8	7
ATAGGCG	30	1.1380099E-4	37.499996	5
TATGCGG	30	1.1380099E-4	37.499996	2
>>END_MODULE