##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936586.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	216859
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95017038721012	33.0	31.0	34.0	30.0	34.0
2	32.051614182487235	33.0	31.0	34.0	30.0	34.0
3	31.94850571108416	33.0	31.0	34.0	30.0	34.0
4	35.71037402182985	37.0	35.0	37.0	35.0	37.0
5	35.76997496068874	37.0	35.0	37.0	35.0	37.0
6	35.72041741408012	37.0	35.0	37.0	35.0	37.0
7	26.742187319871437	35.0	10.0	37.0	0.0	37.0
8	31.01472385282603	35.0	19.0	37.0	17.0	37.0
9	35.83525239902425	39.0	32.0	39.0	32.0	39.0
10	36.61482806800732	37.0	35.0	39.0	33.0	39.0
11	36.9253293614745	39.0	37.0	39.0	33.0	39.0
12	36.849552013059174	39.0	35.0	39.0	33.0	39.0
13	36.707187619605364	39.0	35.0	39.0	33.0	39.0
14	37.737709756108806	40.0	37.0	41.0	33.0	41.0
15	37.79546156719343	40.0	36.0	41.0	33.0	41.0
16	37.83367994872244	40.0	36.0	41.0	33.0	41.0
17	37.680040948265926	40.0	36.0	41.0	33.0	41.0
18	37.59318727836982	39.0	36.0	41.0	33.0	41.0
19	37.465057940874026	39.0	36.0	41.0	32.0	41.0
20	37.53883398890523	39.0	36.0	41.0	32.0	41.0
21	37.51086189643962	39.0	35.0	41.0	32.0	41.0
22	37.58674530455273	39.0	35.0	41.0	33.0	41.0
23	37.614454553419506	39.0	35.0	41.0	33.0	41.0
24	37.486763288588435	39.0	35.0	41.0	33.0	41.0
25	37.41581396206752	39.0	35.0	41.0	33.0	41.0
26	37.23486228378808	39.0	35.0	41.0	32.0	41.0
27	37.13643888425198	39.0	35.0	41.0	32.0	41.0
28	37.08769753618711	39.0	35.0	41.0	32.0	41.0
29	37.022682941450434	39.0	35.0	41.0	32.0	41.0
30	36.88600427005566	39.0	35.0	41.0	31.0	41.0
31	36.77688728620901	39.0	35.0	41.0	31.0	41.0
32	36.583319115185446	39.0	35.0	40.0	30.0	41.0
33	36.44677417123569	39.0	35.0	40.0	30.0	41.0
34	36.30755006709429	39.0	35.0	40.0	30.0	41.0
35	36.18619471638254	39.0	35.0	40.0	30.0	41.0
36	36.07953093945836	38.0	35.0	40.0	29.0	41.0
37	35.956990486906236	38.0	35.0	40.0	29.0	41.0
38	35.70205525249125	38.0	35.0	40.0	28.0	41.0
39	35.59701465007217	38.0	35.0	40.0	27.0	41.0
40	35.35447917771455	38.0	34.0	40.0	26.0	41.0
41	35.242669199802634	38.0	34.0	40.0	26.0	41.0
42	35.26111897592445	38.0	34.0	40.0	26.0	41.0
43	35.26103597268271	38.0	34.0	40.0	26.0	41.0
44	35.1043857990676	38.0	34.0	40.0	26.0	41.0
45	35.001683121290796	38.0	34.0	40.0	25.0	41.0
46	34.86940823299932	38.0	34.0	40.0	24.0	41.0
47	34.65729806002979	37.0	34.0	40.0	24.0	41.0
48	34.55717309403806	37.0	33.0	40.0	23.0	41.0
49	34.44221360423132	37.0	33.0	40.0	24.0	41.0
50	34.21217934233765	37.0	33.0	40.0	23.0	41.0
51	31.90716087411636	35.0	29.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	7.0
10	14.0
11	13.0
12	9.0
13	10.0
14	11.0
15	18.0
16	31.0
17	68.0
18	140.0
19	226.0
20	327.0
21	553.0
22	831.0
23	1103.0
24	1635.0
25	2115.0
26	2611.0
27	3224.0
28	3615.0
29	4233.0
30	5170.0
31	6404.0
32	8310.0
33	11318.0
34	16875.0
35	19248.0
36	20600.0
37	30995.0
38	37889.0
39	39239.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.869435900746566	18.836202325013026	22.979908604208266	12.314453170032142
2	30.27312677822917	27.705559833809062	24.767706205414576	17.253607182547185
3	31.4951189482567	27.783029526097604	24.833186540563222	15.888664985082473
4	27.20984602898658	24.61691698292439	29.626623750916494	18.54661323717254
5	27.706020962929827	28.578016130296646	23.921995397931376	19.79396750884215
6	26.210579224288594	35.72413411479348	24.60538875490526	13.459897906012664
7	59.84487616377462	27.69495386403147	8.39254999792492	4.067619974268995
8	79.94826131265016	7.499804020123675	8.504604374270839	4.047330292955331
9	74.97867277816461	6.374187836336052	9.321264047145842	9.325875338353493
10	44.40396755495507	23.6190335655887	17.065466501275022	14.911532378181214
11	34.39931015083533	23.4723945051854	23.39215803817227	18.736137305807
12	29.668125371785354	22.26884749998847	28.5918500039196	19.47117712430658
13	25.661835570578116	23.915539590240662	31.648674945471484	18.773949893709737
14	21.920695013810818	26.39733651819846	30.77022397041396	20.911744497576766
15	20.64936202786142	23.695580999635705	36.02340691417004	19.631650058332834
16	25.766973010112558	24.45091049944895	28.00806053703098	21.774055953407515
17	25.836603507348094	23.478389183755343	27.813925177188864	22.8710821317077
18	26.81096933952476	23.717254068311668	28.773073748380284	20.698702843783288
19	25.973558856215327	26.15247695507219	25.438649076127806	22.435315112584675
20	26.458205562139455	27.243508454802427	27.65990805085332	18.638377932204797
21	25.09187997731245	28.07123522657579	28.097519586459402	18.739365209652355
22	24.58924923567848	22.358767678537667	29.434332907557447	23.617650178226405
23	23.58352662328979	26.390419581386986	29.138288012026248	20.88776578329698
24	25.074818199844138	24.125353340188788	27.406287034432513	23.393541425534565
25	23.690047450186526	28.512535795147997	25.46677795249448	22.330638802170995
26	22.135581184087354	24.984436892174177	26.939624364218222	25.940357559520244
27	23.067523137153636	25.52026893050323	27.302071853139598	24.110136079203535
28	20.162870805454236	25.877182869975424	28.47380094900373	25.48614537556661
29	24.14472076326092	25.13384272730207	26.203201158356354	24.518235351080655
30	23.905855878704596	27.047067449356494	28.301799786958348	20.745276884980562
31	26.584554941229094	24.354534513209046	25.426659718987914	23.63425082657395
32	28.826103597268272	25.060062067979654	24.931868172406958	21.181966162345116
33	25.077123845447964	24.510857285148415	26.683697702193594	23.72832116721003
34	22.92088407675033	25.983242567751397	28.650413402256763	22.445459953241507
35	21.53057977764354	27.75213387500634	27.231057968541773	23.48622837880835
36	23.862048612231913	26.828031116993067	26.310644243494618	22.9992760272804
37	23.989781378683844	28.676236633019613	25.79786866120382	21.536113327092718
38	22.725365329545927	26.450827496207214	26.70444851262802	24.11935866161884
39	25.106636109176932	25.67613057332184	26.10820855947874	23.109024758022496
40	24.62429504885663	24.11105833744507	28.139021207328263	23.125625406370037
41	20.829202384959814	26.831259020838427	27.33850105368004	25.001037540521722
42	21.734398849021712	23.761983593025885	29.751589742643837	24.752027815308566
43	23.224768167334535	23.11225266186785	28.604761619301023	25.058217551496593
44	22.718909521855217	23.356651095873353	28.070774097455025	25.8536652848164
45	22.15587086540102	23.505595801880485	27.352334927302994	26.9861984054155
46	24.67594151038232	25.33904518604254	26.375663449522502	23.609349854052635
47	20.522090390530252	25.346423251974787	31.134977104939153	22.996509252555807
48	21.86950968140589	26.120659045739398	28.026505701861577	23.983325570993134
49	22.812979862491297	23.290709631603946	30.501385693007897	23.394924812896857
50	21.696586261118973	24.67732489774462	28.924785229112004	24.701303612024404
51	21.971880346215745	23.95934685671335	26.75563384503295	27.313138952037956
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	237.5
2	389.0
3	771.5
4	1154.0
5	825.5
6	497.0
7	434.0
8	371.0
9	365.5
10	360.0
11	365.0
12	370.0
13	385.5
14	401.0
15	407.0
16	413.0
17	431.5
18	450.0
19	473.5
20	497.0
21	591.5
22	686.0
23	816.5
24	947.0
25	1121.0
26	1511.0
27	1727.0
28	2063.5
29	2400.0
30	2767.5
31	3135.0
32	3715.0
33	4295.0
34	4791.0
35	5287.0
36	5814.5
37	6342.0
38	7072.5
39	7803.0
40	8588.0
41	9373.0
42	10331.0
43	11289.0
44	12590.5
45	13892.0
46	18896.5
47	23901.0
48	20199.0
49	16497.0
50	16288.0
51	16079.0
52	15162.0
53	14245.0
54	13598.5
55	12952.0
56	12525.5
57	12099.0
58	11728.0
59	11357.0
60	10654.0
61	9951.0
62	8851.0
63	7751.0
64	6851.0
65	5951.0
66	5192.5
67	4434.0
68	3694.5
69	2955.0
70	2432.5
71	1910.0
72	1602.5
73	1295.0
74	1083.5
75	689.0
76	506.0
77	402.0
78	298.0
79	220.5
80	143.0
81	121.0
82	99.0
83	71.0
84	43.0
85	34.5
86	26.0
87	20.0
88	14.0
89	14.5
90	15.0
91	8.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	216859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.84922922267464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.68263032253316	30.116804006289797
2	10.178670957293539	8.92653751977091
3	5.67456436466122	7.464758206945526
4	3.7090786720089177	6.505609635754108
5	2.824662691527063	6.192964091875366
6	2.0874741037532467	5.492047828312406
7	1.642637052928248	5.041985806445663
8	1.219884111009454	4.279278240700179
9	1.0074560158164285	3.9758552792367388
>10	2.9340316118244627	16.431413960223
>50	0.018929236205319114	0.5842505960093886
>100	0.014722739270803758	1.6789711287057487
>500	0.00210324846725768	0.4883357388902466
>1k	0.0031548727008865194	2.8211879608409154
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC	2236	1.0310847140307757	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG	2183	1.0066448706302251	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC	1699	0.7834583761799142	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	533	0.2457818213678012	No Hit
GAATCTATCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC	526	0.24255391752244548	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCT	484	0.22318649445031102	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG	462	0.2130416537934787	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT	446	0.205663587861237	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	441	0.20335794225741147	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC	412	0.18998519775522343	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTC	342	0.15770615930166607	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07101388459782623	0.0
2	0.0	0.0	0.0	0.489719126252542	0.0
3	0.0	0.0	0.0	0.5939343075454558	0.0
4	0.0	0.0	0.0	0.9457758266892312	0.0
5	0.0	0.0	0.0	2.4910195103730994	0.0
6	0.0	0.0	0.0	2.8008982795272503	0.0
7	0.0	0.0	0.0	3.2869283728136716	0.0
8	0.0	0.0	0.0	3.9864612490143365	0.0
9	0.0	0.0	0.0	4.2059587104985265	0.0
10	0.0	0.0	0.0	5.925048072710839	0.0
11	0.0	0.0	0.0	6.511604314324054	0.0
12	0.0	0.0	0.0	8.058692514490982	0.0
13	0.0	0.0	0.0	8.308163368824905	0.0
14	0.0	0.0	0.0	8.439124039122195	0.0
15	0.0	0.0	0.0	8.69873973411295	0.0
16	0.0	0.0	0.0	9.026602538976938	0.0
17	0.0	0.0	0.0	9.382133091086835	0.0
18	0.0	0.0	0.0	9.778704134944826	0.0
19	0.0	0.0	0.0	10.018030148621916	0.0
20	0.0	0.0	0.0	10.261967453506657	0.0
21	0.0	0.0	0.0	10.553862186950969	0.0
22	0.0	0.0	0.0	10.891408703351026	0.0
23	0.0	0.0	0.0	11.187914728002989	0.0
24	0.0	0.0	0.0	11.442919131786091	0.0
25	0.0	0.0	0.0	11.679939499859355	0.0
26	0.0	0.0	0.0	11.920187771777975	0.0
27	0.0	0.0	0.0	12.201015406323926	0.0
28	0.0	0.0	0.0	12.466625779884625	0.0
29	0.0	0.0	0.0	12.740075348498333	0.0
30	0.0	0.0	0.0	13.086844447313693	0.0
31	0.0	0.0	0.0	13.372283373067292	0.0
32	0.0	0.0	0.0	13.654494394975536	0.0
33	0.0	0.0	0.0	13.940394449849903	0.0
34	0.0	0.0	0.0	14.252578864607878	0.0
35	0.0	0.0	0.0	14.58413070243799	0.0
36	0.0	0.0	0.0	14.856196883689401	0.0
37	0.0	0.0	0.0	15.14947500449601	0.0
38	0.0	0.0	0.0	15.535901207697167	0.0
39	0.0	0.0	0.0	16.25710715257379	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.020617E-4	45.000004	25
CTCACGA	20	7.020617E-4	45.000004	24
CTCGTTT	20	7.020617E-4	45.000004	7
CGACGGT	20	7.020617E-4	45.000004	28
CAACGAA	20	7.020617E-4	45.000004	16
CCAATCC	20	7.020617E-4	45.000004	43
TACGCGG	30	2.1573323E-6	45.000004	2
TCTCGTT	20	7.020617E-4	45.000004	6
GTAAGGC	20	7.020617E-4	45.000004	4
AATCCTA	70	0.0	41.785713	13
GATACCT	235	0.0	41.17021	5
TGAAACG	50	1.0732037E-9	40.5	4
TACGGCT	245	0.0	40.408165	7
TGATACC	240	0.0	40.312504	4
CGGCTGT	300	0.0	39.75	9
ATCTATC	125	0.0	39.6	3
TATCTCT	145	0.0	38.793106	6
ATGATAC	605	0.0	37.561985	3
ACAACGA	60	1.546141E-10	37.500004	13
CGACCCG	30	1.13687E-4	37.500004	32
>>END_MODULE