##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936583.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268884
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89142157956591	33.0	31.0	34.0	30.0	34.0
2	31.989010130762708	33.0	31.0	34.0	30.0	34.0
3	31.421531217922972	33.0	31.0	34.0	28.0	34.0
4	35.426351140268665	37.0	35.0	37.0	33.0	37.0
5	35.67183990122135	37.0	35.0	37.0	35.0	37.0
6	35.68977700420999	37.0	35.0	37.0	35.0	37.0
7	26.748783862185924	35.0	10.0	37.0	0.0	37.0
8	31.044669820443016	35.0	19.0	37.0	17.0	37.0
9	35.88585412296752	39.0	32.0	39.0	32.0	39.0
10	36.70013834962288	38.0	35.0	39.0	33.0	39.0
11	37.069788458963714	39.0	37.0	39.0	34.0	39.0
12	37.18304547685991	39.0	37.0	39.0	34.0	39.0
13	37.19579818806623	39.0	37.0	39.0	34.0	39.0
14	38.531061721783374	40.0	38.0	41.0	34.0	41.0
15	38.53006872852234	40.0	38.0	41.0	34.0	41.0
16	38.462102616741795	40.0	38.0	41.0	34.0	41.0
17	38.331008910905815	40.0	38.0	41.0	34.0	41.0
18	38.13869549694292	40.0	37.0	41.0	33.0	41.0
19	37.8837640023207	40.0	37.0	41.0	33.0	41.0
20	37.85444652712694	40.0	36.0	41.0	34.0	41.0
21	37.828301423662246	40.0	36.0	41.0	33.0	41.0
22	37.835724699126764	40.0	36.0	41.0	33.0	41.0
23	37.79230820725666	40.0	36.0	41.0	33.0	41.0
24	37.69384195415123	40.0	35.0	41.0	33.0	41.0
25	37.56686154624299	39.0	35.0	41.0	33.0	41.0
26	37.4346074887312	39.0	35.0	41.0	33.0	41.0
27	37.34245622647685	39.0	35.0	41.0	33.0	41.0
28	37.28632793323515	39.0	35.0	41.0	33.0	41.0
29	37.22960458785201	39.0	35.0	41.0	33.0	41.0
30	37.14684027312893	39.0	35.0	41.0	32.0	41.0
31	36.943429136728106	39.0	35.0	41.0	32.0	41.0
32	36.68811085821395	39.0	35.0	41.0	31.0	41.0
33	36.43590916529061	39.0	35.0	41.0	30.0	41.0
34	36.064827211734425	39.0	35.0	41.0	28.0	41.0
35	35.789712292289614	39.0	35.0	41.0	26.0	41.0
36	35.52734636497523	39.0	35.0	41.0	25.0	41.0
37	35.32752413680249	39.0	35.0	41.0	24.0	41.0
38	35.18881004448015	39.0	35.0	40.0	23.0	41.0
39	35.094181877687035	39.0	35.0	40.0	23.0	41.0
40	34.81508010889454	38.0	34.0	40.0	22.0	41.0
41	34.74975454099165	38.0	34.0	40.0	22.0	41.0
42	34.591567367340566	38.0	34.0	40.0	21.0	41.0
43	34.60425685425685	38.0	34.0	40.0	21.0	41.0
44	34.41269841269841	38.0	34.0	40.0	20.0	41.0
45	34.33048824028206	38.0	34.0	40.0	20.0	41.0
46	34.169630770146235	38.0	33.0	40.0	20.0	41.0
47	33.995250740096104	38.0	33.0	40.0	19.0	41.0
48	33.91067151634162	38.0	33.0	40.0	18.0	41.0
49	33.734487734487736	37.0	33.0	40.0	18.0	41.0
50	33.565399949420566	37.0	33.0	40.0	17.0	41.0
51	31.470247392927806	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	16.0
10	22.0
11	16.0
12	11.0
13	11.0
14	15.0
15	17.0
16	39.0
17	68.0
18	131.0
19	225.0
20	409.0
21	618.0
22	1055.0
23	1715.0
24	2650.0
25	4167.0
26	5233.0
27	5649.0
28	5632.0
29	5507.0
30	5997.0
31	6851.0
32	8576.0
33	11498.0
34	16727.0
35	21173.0
36	23949.0
37	38289.0
38	49083.0
39	53521.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12522872316687	17.43279629877568	20.599961321610806	19.842013656446646
2	35.4677109831749	26.183409946296543	22.76595111646658	15.582927954061976
3	29.407848737745645	25.523273976882226	30.37257702206156	14.696300263310572
4	25.271864447122177	23.3955906636319	34.548355424644086	16.784189464601837
5	24.692060516802787	27.80678656967317	29.683060353163448	17.8180925603606
6	23.98394846848455	33.6342065723509	29.784219217208907	12.597625741955639
7	55.6533672513054	27.57211288139123	13.226149566355753	3.5483703009476204
8	75.48459558768837	6.312015590366106	14.55534728730605	3.648041534639473
9	69.49204861575996	7.395382395382395	15.668466699394534	7.444102289463114
10	36.48115916157153	26.379777152973027	23.160916975349966	13.978146710105474
11	27.81534044420642	24.64743160619449	30.039719730441377	17.497508219157705
12	25.29231936448431	22.853349399741152	33.26973713571652	18.58459410005802
13	21.763660165722023	23.932997128873417	35.53762960979456	18.765713095610003
14	19.634117314529686	26.33737968789515	33.289448237901844	20.739054759673316
15	18.400871751387214	25.497240445694054	37.956144657175585	18.145743145743147
16	21.352702280537333	25.396453489236993	33.49883221017242	19.752012020053257
17	20.65723509022478	24.28630933785573	33.34188720786659	21.7145683640529
18	21.5617143452195	25.07066244179646	33.22659585546183	20.141027357522205
19	22.179452849555943	26.493208967435773	30.71026911233097	20.617069070677317
20	23.313399086594963	26.289031701402838	32.64158521890481	17.755983993097395
21	22.799794707011202	26.255560018446616	32.217610568126034	18.72703470641615
22	21.776676931316107	23.295175614763245	31.930497909879353	22.997649544041295
23	21.26641972002797	25.974397881614376	33.10684161199625	19.652340786361407
24	21.935481471563946	23.831466357239552	33.46945151068862	20.763600660507876
25	20.4154951577632	27.78558783713423	31.016349057586172	20.7825679475164
26	20.377560583746153	26.49023370672855	31.28189107570551	21.85031463381979
27	21.587375968819266	26.56461522440904	31.049448832953985	20.798559973817706
28	19.305722913970335	27.123220422189494	33.21655435057497	20.3545023132652
29	20.758765861858645	27.56244328409277	31.006679460287707	20.67211139376088
30	20.465330774609125	26.989705597953023	32.2280239806013	20.316939646836556
31	21.83841359099091	25.72001309114711	31.78619776557921	20.655375552282766
32	22.733223248687164	26.73978369854659	30.583820532274142	19.94317252049211
33	21.170095654631737	26.52184585174276	31.12345844304607	21.184600050579434
34	20.581365942190686	27.196858124693176	31.42284405171003	20.79893188140611
35	22.766323024054984	26.17262462623287	29.590827271239643	21.470225078472502
36	22.144493536246113	27.829472932565718	29.698308564287945	20.327724966900224
37	21.190922479582273	27.51112003689323	30.835973877210993	20.461983606313503
38	21.41220749468172	26.441885720236236	30.257285669656802	21.888621115425238
39	23.31451480936017	25.344758334449057	29.351690691896877	21.989036164293896
40	23.110337543327233	26.474985495604052	31.09296202079707	19.321714940271644
41	21.363859508189407	27.528971601136547	30.03488493179215	21.072283958881897
42	21.809776706683923	27.276074441022896	30.504976123532824	20.409172728760357
43	22.861159459097603	26.335892057541542	29.321938084824684	21.48101039853617
44	21.399562636676038	26.65498876839083	29.270614837625146	22.674833757307983
45	20.655747459871172	25.962124931197096	28.525683938055074	24.85644367087666
46	21.737626634533854	27.63273381830083	28.2389431873968	22.390696359768526
47	20.644590232219098	25.87286710998051	32.588030526174855	20.894512131625532
48	21.536796536796537	25.42992517219321	30.912958748010293	22.120319542999955
49	20.342973178024724	25.5961678642091	31.967688668719596	22.093170289046576
50	20.402106484580713	25.721500721500725	31.1435414528198	22.732851341098765
51	20.03094271135508	25.725219797384746	29.729920709302153	24.51391678195802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	331.5
2	537.0
3	4186.0
4	7835.0
5	5450.5
6	3066.0
7	2671.0
8	2276.0
9	2193.0
10	2110.0
11	1978.5
12	1847.0
13	1828.0
14	1809.0
15	1733.0
16	1657.0
17	1581.5
18	1506.0
19	1458.0
20	1410.0
21	1440.5
22	1471.0
23	1573.5
24	1676.0
25	1956.0
26	2516.0
27	2796.0
28	3329.5
29	3863.0
30	4466.5
31	5070.0
32	5717.5
33	6365.0
34	7153.5
35	7942.0
36	8492.0
37	9042.0
38	9983.0
39	10924.0
40	11679.0
41	12434.0
42	13250.5
43	14067.0
44	15663.0
45	17259.0
46	21270.5
47	25282.0
48	22546.0
49	19810.0
50	19386.0
51	18962.0
52	17432.0
53	15902.0
54	14536.5
55	13171.0
56	12493.5
57	11816.0
58	11311.0
59	10806.0
60	10355.5
61	9905.0
62	8832.5
63	7760.0
64	6531.0
65	5302.0
66	4485.5
67	3669.0
68	3094.5
69	2520.0
70	2168.5
71	1817.0
72	1453.5
73	1090.0
74	897.0
75	543.0
76	382.0
77	324.0
78	266.0
79	228.0
80	190.0
81	144.5
82	99.0
83	74.0
84	49.0
85	39.0
86	29.0
87	20.5
88	12.0
89	7.5
90	3.0
91	6.0
92	9.0
93	6.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	268884.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.73920298085935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.54301346504029	35.792962790841734
2	11.727392024448276	11.900770487291844
3	6.037071967503102	9.189486599075876
4	3.779239514794818	7.670224034178348
5	2.4292836775348876	6.162995880626557
6	1.6332750651445245	4.9722645032366595
7	1.1746716605735266	4.172133267519229
8	0.8496618357172182	3.4488931478036404
9	0.5917395105124451	2.7021952022116777
>10	1.1973046281793733	7.964768064360675
>50	0.019771756174348912	0.7271365052572253
>100	0.013181170782899276	1.592120554433935
>500	0.0014645745314332528	0.44438183969731293
>1k	0.0029291490628665057	3.2596671234653236
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3895	1.4485800568274796	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	2175	0.8088990047752934	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	1477	0.5493075080703947	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	1226	0.4559587033813838	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	662	0.24620282352241116	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCT	534	0.19859865220689965	No Hit
GAATCTATCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTC	423	0.15731690989422947	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	367	0.1364900849436932	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	338	0.1257047648800226	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCG	337	0.1253328572916202	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	334	0.12421713452641288	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTT	300	0.11157227652073014	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCT	277	0.10301840198747415	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGC	274	0.10190267922226684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05578613826036506	0.0
2	0.0	0.0	0.0	0.3622379911039705	0.0
3	0.0	0.0	0.0	0.5318278514154803	0.0
4	0.0	0.0	0.0	0.8936939349310483	0.0
5	0.0	0.0	0.0	2.2236354710581514	0.0
6	0.0	0.0	0.0	2.6937266627988277	0.0
7	0.0	0.0	0.0	3.100965472099493	0.0
8	0.0	0.0	0.0	3.676306511358058	0.0
9	0.0	0.0	0.0	3.9477990508918346	0.0
10	0.0	0.0	0.0	5.107778819119026	0.0
11	0.0	0.0	0.0	5.5291501167789825	0.0
12	0.0	0.0	0.0	6.478258282381994	0.0
13	0.0	0.0	0.0	6.704006188542271	0.0
14	0.0	0.0	0.0	6.8300828610106965	0.0
15	0.0	0.0	0.0	7.0245905297451685	0.0
16	0.0	0.0	0.0	7.283438211273263	0.0
17	0.0	0.0	0.0	7.547492599038991	0.0
18	0.0	0.0	0.0	7.843531039407328	0.0
19	0.0	0.0	0.0	8.028369110843338	0.0
20	0.0	0.0	0.0	8.219529611282189	0.0
21	0.0	0.0	0.0	8.418872078665894	0.0
22	0.0	0.0	0.0	8.66730634771872	0.0
23	0.0	0.0	0.0	8.924666398893203	0.0
24	0.0	0.0	0.0	9.105785394445189	0.0
25	0.0	0.0	0.0	9.293598726588417	0.0
26	0.0	0.0	0.0	9.469882923491172	0.0
27	0.0	0.0	0.0	9.646539027982326	0.0
28	0.0	0.0	0.0	9.815756980705434	0.0
29	0.0	0.0	0.0	10.005429850790675	0.0
30	0.0	0.0	0.0	10.237872093542197	0.0
31	0.0	0.0	0.0	10.434611207807084	0.0
32	0.0	0.0	0.0	10.656268130494935	0.0
33	0.0	0.0	0.0	10.870858809003138	0.0
34	0.0	0.0	0.0	11.07094509156365	0.0
35	0.0	0.0	0.0	11.279585248657414	0.0
36	0.0	0.0	0.0	11.496407372696032	0.0
37	0.0	0.0	0.0	11.692030764195712	0.0
38	0.0	0.0	0.0	11.90699335029232	0.0
39	0.0	0.0	0.0	12.115261599797682	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGAC	20	7.0236356E-4	45.000004	14
CGCACTT	80	0.0	45.000004	35
CGTTTAG	20	7.0236356E-4	45.000004	2
CCGCACT	80	0.0	45.000004	34
ATGGGCG	35	1.2072815E-7	45.0	5
CTAGCGG	35	1.2072815E-7	45.0	2
TCGGGAC	30	2.1592787E-6	44.999996	5
CTACGAA	30	2.1592787E-6	44.999996	11
TACGAAT	30	2.1592787E-6	44.999996	12
CGAATAT	30	2.1592787E-6	44.999996	14
CGTTTTA	285	0.0	44.210526	1
CGTTTTT	1805	0.0	42.880886	1
CAACGCA	85	0.0	42.35294	17
AACGCAA	85	0.0	42.35294	18
CGCAAGC	85	0.0	42.35294	20
CCCGCAC	85	0.0	42.35294	33
AACGGCT	45	1.918852E-8	40.0	7
TCAACGC	90	0.0	40.0	16
CTACGGG	40	3.4450568E-7	39.375004	3
CGGTTTT	145	0.0	38.793102	1
>>END_MODULE