##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936581.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322980
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094981113381635	33.0	31.0	34.0	30.0	34.0
2	32.23366152702954	33.0	31.0	34.0	30.0	34.0
3	31.985748343550686	33.0	31.0	34.0	30.0	34.0
4	35.779797510681774	37.0	35.0	37.0	35.0	37.0
5	35.89237723698062	37.0	35.0	37.0	35.0	37.0
6	35.88481020496625	37.0	35.0	37.0	35.0	37.0
7	26.819264350733793	36.0	10.0	37.0	0.0	37.0
8	31.20516131029785	37.0	19.0	37.0	17.0	37.0
9	36.091281193881976	39.0	32.0	39.0	32.0	39.0
10	36.80813672673231	38.0	35.0	39.0	34.0	39.0
11	37.16541271905381	39.0	37.0	39.0	34.0	39.0
12	37.23694346399158	39.0	37.0	39.0	34.0	39.0
13	37.24090346151465	39.0	37.0	39.0	34.0	39.0
14	38.40535017648151	40.0	38.0	41.0	34.0	41.0
15	38.50262245340269	40.0	38.0	41.0	34.0	41.0
16	38.44431543748839	40.0	38.0	41.0	34.0	41.0
17	38.378280388878565	40.0	38.0	41.0	34.0	41.0
18	38.25284537742275	40.0	37.0	41.0	34.0	41.0
19	38.10541519598737	40.0	37.0	41.0	34.0	41.0
20	38.12698309492848	40.0	37.0	41.0	34.0	41.0
21	38.075670320143665	40.0	37.0	41.0	34.0	41.0
22	38.08816025760109	40.0	37.0	41.0	34.0	41.0
23	38.01806303795901	40.0	36.0	41.0	34.0	41.0
24	37.967121803207625	40.0	36.0	41.0	34.0	41.0
25	37.85438107622763	40.0	36.0	41.0	33.0	41.0
26	37.75024150102173	40.0	36.0	41.0	33.0	41.0
27	37.64151031023593	40.0	36.0	41.0	33.0	41.0
28	37.615697566412784	40.0	36.0	41.0	33.0	41.0
29	37.58152207567032	40.0	36.0	41.0	33.0	41.0
30	37.48651928912007	40.0	36.0	41.0	33.0	41.0
31	37.35111462010032	40.0	35.0	41.0	33.0	41.0
32	37.19022540095362	40.0	35.0	41.0	32.0	41.0
33	37.0633011331971	40.0	35.0	41.0	32.0	41.0
34	36.88079757260512	39.0	35.0	41.0	31.0	41.0
35	36.70417672920924	39.0	35.0	41.0	30.0	41.0
36	36.527639482320886	39.0	35.0	41.0	30.0	41.0
37	36.45782401387083	39.0	35.0	41.0	30.0	41.0
38	36.27019939315128	39.0	35.0	41.0	30.0	41.0
39	36.19908663075113	39.0	35.0	41.0	29.0	41.0
40	35.999495324787915	39.0	35.0	41.0	28.0	41.0
41	35.892689949842094	39.0	35.0	41.0	28.0	41.0
42	35.82684686358289	39.0	35.0	40.0	27.0	41.0
43	35.819072388383184	39.0	35.0	40.0	28.0	41.0
44	35.6442411294817	39.0	35.0	40.0	27.0	41.0
45	35.539466840052015	38.0	35.0	40.0	27.0	41.0
46	35.44311102854666	38.0	35.0	40.0	26.0	41.0
47	35.31335376803517	38.0	34.0	40.0	26.0	41.0
48	35.23060561025451	38.0	34.0	40.0	26.0	41.0
49	35.103941420521394	38.0	34.0	40.0	25.0	41.0
50	34.92703572976655	38.0	34.0	40.0	24.0	41.0
51	32.98941730138089	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	20.0
9	30.0
10	33.0
11	25.0
12	14.0
13	14.0
14	12.0
15	23.0
16	35.0
17	74.0
18	124.0
19	228.0
20	366.0
21	597.0
22	917.0
23	1394.0
24	2061.0
25	2983.0
26	3841.0
27	4254.0
28	4643.0
29	4956.0
30	5913.0
31	7264.0
32	9709.0
33	13307.0
34	20576.0
35	25402.0
36	29113.0
37	48729.0
38	63590.0
39	72719.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.96971948727475	19.503994055359463	24.8758437054926	14.65044275187318
2	32.45340268747291	24.17208495882098	26.760170908415382	16.61434144529073
3	31.99547959625983	23.49433401449006	28.808904576134747	15.701281813115365
4	27.333581026688957	25.608396804755714	28.283175428819124	18.774846739736205
5	25.22725865378661	29.67892748777014	26.73478233946374	18.359031518979503
6	26.27035729766549	34.28602390240882	26.083348814168062	13.36026998575763
7	61.98619109542387	27.835160071831073	6.75707474147006	3.4215740912750015
8	85.57433896835718	4.009536194191591	7.66920552356183	2.746919313889405
9	79.84426280265032	6.457985014551984	9.287262369186946	4.4104898136107495
10	39.54672115920491	29.41234751377794	16.75955167502632	14.281379651990836
11	29.582946312465168	26.910954238652547	25.839990092265776	17.66610935661651
12	27.01065081429191	25.078642640411168	28.97052449068054	18.940182054616386
13	24.89875534088798	24.641154251037218	30.881478729333082	19.578611678741716
14	21.379651990835345	26.58430862592111	31.325469069292218	20.71057031395133
15	21.66140318285962	25.303424360641525	32.8633351910335	20.171837265465353
16	25.657935475880862	25.630998823456565	29.471484302433588	19.239581398228992
17	25.623258406093257	24.947674778624062	28.876401015542758	20.552665799739923
18	26.595454826924268	24.31172208805499	29.535884574896276	19.556938510124468
19	24.832497368258096	26.706297603566785	28.209486655520465	20.251718372654654
20	26.195739674283235	26.675645550808095	28.316304415134063	18.8123103597746
21	25.651123908601154	26.34683262121494	29.306768220942477	18.69527524924144
22	24.883274506161374	24.173013808904575	29.55322310979008	21.39048857514397
23	23.39990092265775	26.01027927425847	29.659112019320084	20.9307077837637
24	23.071397609759117	25.120750510867545	30.79757260511487	21.010279274258465
25	23.125270914607714	26.999814229983283	28.56430738745433	21.31060746795467
26	22.641340021053935	27.013127747848166	28.639853860920176	21.70567837017772
27	22.260511486779365	25.94773670196297	30.127562078147253	21.66418973311041
28	20.80902842281256	27.02086816521147	30.096910025388567	22.073193386587405
29	22.13542634218837	27.138212892439164	29.40894173013809	21.31741903523438
30	23.284723512291784	25.920180816149607	29.612669515140254	21.182426156418355
31	23.927487770140566	26.209362808842652	28.558734286952753	21.30441513406403
32	24.308625921109666	26.462010031580903	28.583194005820793	20.646170041488638
33	24.290048919437737	26.25766301318967	27.867979441451485	21.584308625921107
34	22.371044646727352	26.787417177534213	29.826924267756517	21.014613907981918
35	22.249984519165274	27.366710013003903	29.159081057650628	21.2242244101802
36	23.379156604124095	28.29463124651681	27.37197349681095	20.954238652548145
37	22.924020063161805	28.215059756022043	27.990897269180753	20.870022911635395
38	23.24168679175181	28.015047371354264	27.96860486717444	20.774660969719488
39	23.272029227815963	26.84562511610626	27.970772184036164	21.911573472041614
40	24.523499907114992	25.978698371416186	28.67700786426404	20.82079385720478
41	22.05368753483188	26.83571738188123	28.998080376493903	22.11251470679299
42	22.39240819865007	26.847173199578922	28.805498792494895	21.954919809276117
43	22.25679608644498	25.985819555390428	28.89683571738188	22.86054864078271
44	22.44349495324788	26.46015233141371	28.725927302000127	22.37042541333829
45	22.586228249427208	26.401944392841664	28.144776766363243	22.86705059136789
46	22.462691188308874	27.03789708341074	27.95529134930955	22.544120378970835
47	21.990835345841848	26.950275558858134	29.593782896773796	21.465106198526225
48	22.114372406960182	27.249055669081677	28.856895163787232	21.779676760170908
49	22.58468016595455	26.37315004025017	29.117592420583314	21.924577373211964
50	21.935413957520588	26.84098086568828	28.923462753111647	22.300142423679485
51	21.97845067806056	26.049910211158583	28.788469874295625	23.18316923648523
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	312.5
2	523.0
3	2292.5
4	4062.0
5	2783.0
6	1504.0
7	1299.0
8	1094.0
9	1068.5
10	1043.0
11	1003.0
12	963.0
13	968.5
14	974.0
15	954.5
16	935.0
17	948.0
18	961.0
19	960.0
20	959.0
21	1087.0
22	1215.0
23	1412.0
24	1609.0
25	1956.5
26	2742.5
27	3181.0
28	3964.0
29	4747.0
30	5457.0
31	6167.0
32	7228.5
33	8290.0
34	9521.5
35	10753.0
36	11433.5
37	12114.0
38	13552.0
39	14990.0
40	15914.0
41	16838.0
42	17703.0
43	18568.0
44	19429.0
45	20290.0
46	21633.5
47	22977.0
48	23746.0
49	24515.0
50	24412.5
51	24310.0
52	22938.5
53	21567.0
54	20601.0
55	19635.0
56	18401.5
57	17168.0
58	16086.0
59	15004.0
60	13485.0
61	11966.0
62	10776.0
63	9586.0
64	8298.0
65	7010.0
66	6065.5
67	5121.0
68	4304.0
69	3487.0
70	2980.5
71	2474.0
72	2098.0
73	1722.0
74	1378.5
75	793.5
76	552.0
77	446.5
78	341.0
79	247.5
80	154.0
81	113.0
82	72.0
83	58.5
84	45.0
85	37.5
86	30.0
87	19.5
88	9.0
89	7.0
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322980.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.70359186364989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.43188027957126	29.250400708891554
2	11.340208356409972	10.138960920274068
3	6.56370189664925	8.802631521074183
4	4.386035356012478	7.842861378188807
5	3.1503678468394534	7.041637922273821
6	2.371498974989057	6.360871336978493
7	1.7656205376601581	5.525070593114646
8	1.3081666016961888	4.678379668150742
9	1.0034329139544942	4.037134990317716
>10	2.662521317582996	14.978558831925595
>50	0.011043945756441416	0.32707892892491247
>100	0.00483172626844312	0.39465466990090853
>500	0.0	0.0
>1k	6.902466097775885E-4	0.6217585299846213
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2015	0.6238776394823209	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	348	0.10774660969719488	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03839247012198898	0.0
2	0.0	0.0	0.0	0.15480834726608458	0.0
3	0.0	0.0	0.0	0.23252213759365906	0.0
4	0.0	0.0	0.0	0.30992631122670133	0.0
5	0.0	0.0	0.0	0.5696947179391912	0.0
6	0.0	0.0	0.0	0.8229611740665057	0.0
7	0.0	0.0	0.0	1.0362870765991703	0.0
8	0.0	0.0	0.0	1.4579850145519846	0.0
9	0.0	0.0	0.0	1.6942225524800296	0.0
10	0.0	0.0	0.0	2.1255186079633415	0.0
11	0.0	0.0	0.0	2.5354511115239333	0.0
12	0.0	0.0	0.0	2.8924391603195243	0.0
13	0.0	0.0	0.0	3.0518917580035914	0.0
14	0.0	0.0	0.0	3.1537556505046753	0.0
15	0.0	0.0	0.0	3.2639791937581273	0.0
16	0.0	0.0	0.0	3.4624434949532477	0.0
17	0.0	0.0	0.0	3.690940615517989	0.0
18	0.0	0.0	0.0	3.962164839928169	0.0
19	0.0	0.0	0.0	4.1259520713356865	0.0
20	0.0	0.0	0.0	4.3089355378041985	0.0
21	0.0	0.0	0.0	4.512972939500898	0.0
22	0.0	0.0	0.0	4.725679608644498	0.0
23	0.0	0.0	0.0	4.94581707845687	0.0
24	0.0	0.0	0.0	5.139327512539476	0.0
25	0.0	0.0	0.0	5.330980246454889	0.0
26	0.0	0.0	0.0	5.510248312589015	0.0
27	0.0	0.0	0.0	5.693851012446591	0.0
28	0.0	0.0	0.0	5.884265279583875	0.0
29	0.0	0.0	0.0	6.076846863582884	0.0
30	0.0	0.0	0.0	6.322682519041427	0.0
31	0.0	0.0	0.0	6.526100687349062	0.0
32	0.0	0.0	0.0	6.7394265898817265	0.0
33	0.0	0.0	0.0	6.9508947922471975	0.0
34	0.0	0.0	0.0	7.1688649451978455	0.0
35	0.0	0.0	0.0	7.39798129915165	0.0
36	0.0	0.0	0.0	7.594278283485045	0.0
37	0.0	0.0	0.0	7.82184655396619	0.0
38	3.0961669453216916E-4	0.0	0.0	8.082853427456808	0.0
39	3.0961669453216916E-4	0.0	0.0	8.494334014490061	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	20	7.0257456E-4	45.000004	20
ATAGCGG	40	6.7884685E-9	45.000004	2
ACGGGTA	25	3.884556E-5	45.000004	5
GGTATTC	20	7.0257456E-4	45.000004	9
TAATCGT	20	7.0257456E-4	45.000004	21
CTACGAA	60	0.0	44.999996	11
GCTACGA	60	0.0	44.999996	10
AGGCGAT	135	0.0	43.333336	7
CGTTTTT	815	0.0	42.515335	1
TACGAAT	65	0.0	41.53846	12
GACACGA	95	0.0	40.263157	25
CGAGACA	95	0.0	40.263157	22
TCGTAAC	45	1.9208528E-8	40.000004	34
CTAACGG	40	3.4479308E-7	39.375004	2
CGTTTTA	110	0.0	38.863636	1
CGAATAT	70	0.0	38.571426	14
TGTACGG	35	6.234348E-6	38.571426	2
ACACGAC	100	0.0	38.250004	26
CACGACC	100	0.0	38.250004	27
TCGATCA	30	1.13810514E-4	37.499996	17
>>END_MODULE