##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936580.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126476
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00143110155286	33.0	31.0	34.0	30.0	34.0
2	32.13148739681837	33.0	31.0	34.0	30.0	34.0
3	32.01910243840729	33.0	31.0	34.0	30.0	34.0
4	35.756530883329646	37.0	35.0	37.0	35.0	37.0
5	35.8378822859673	37.0	35.0	37.0	35.0	37.0
6	35.8077342736962	37.0	35.0	37.0	35.0	37.0
7	26.568107783294856	35.0	10.0	37.0	0.0	37.0
8	31.011266959739398	35.0	19.0	37.0	17.0	37.0
9	35.896707675764574	39.0	32.0	39.0	32.0	39.0
10	36.674119991144565	38.0	35.0	39.0	33.0	39.0
11	37.0041826117208	39.0	37.0	39.0	34.0	39.0
12	37.01451658812739	39.0	37.0	39.0	34.0	39.0
13	36.91452133211044	39.0	37.0	39.0	33.0	39.0
14	38.074346121003195	40.0	37.0	41.0	33.0	41.0
15	38.170641070242574	40.0	37.0	41.0	33.0	41.0
16	38.14042189822575	40.0	37.0	41.0	33.0	41.0
17	38.00181852683513	40.0	37.0	41.0	33.0	41.0
18	37.9087494860685	40.0	36.0	41.0	33.0	41.0
19	37.79760586988836	40.0	36.0	41.0	33.0	41.0
20	37.87705177266833	40.0	36.0	41.0	33.0	41.0
21	37.806706410702425	40.0	36.0	41.0	33.0	41.0
22	37.79707612511464	40.0	36.0	41.0	33.0	41.0
23	37.737175432493125	40.0	36.0	41.0	33.0	41.0
24	37.70146905341725	40.0	36.0	41.0	33.0	41.0
25	37.57420380151175	39.0	35.0	41.0	33.0	41.0
26	37.40030519624276	39.0	35.0	41.0	33.0	41.0
27	37.300594579208706	39.0	35.0	41.0	32.0	41.0
28	37.205430279262465	39.0	35.0	41.0	32.0	41.0
29	37.17631012998513	39.0	35.0	41.0	32.0	41.0
30	37.080481672412155	39.0	35.0	41.0	32.0	41.0
31	36.85530061039248	39.0	35.0	41.0	31.0	41.0
32	36.70431544324615	39.0	35.0	41.0	31.0	41.0
33	36.618409816882256	39.0	35.0	41.0	31.0	41.0
34	36.390864669976914	39.0	35.0	41.0	30.0	41.0
35	36.27202789462032	39.0	35.0	41.0	30.0	41.0
36	36.03000569277966	39.0	35.0	40.0	29.0	41.0
37	35.97102217021411	38.0	35.0	40.0	29.0	41.0
38	35.72952971314716	38.0	35.0	40.0	28.0	41.0
39	35.74388816850628	38.0	35.0	40.0	28.0	41.0
40	35.52792624687688	38.0	35.0	40.0	27.0	41.0
41	35.43943514975173	38.0	34.0	40.0	27.0	41.0
42	35.346587494860685	38.0	34.0	40.0	26.0	41.0
43	35.27081027230463	38.0	34.0	40.0	26.0	41.0
44	35.00702109491129	38.0	34.0	40.0	25.0	41.0
45	34.828378506594134	38.0	34.0	40.0	24.0	41.0
46	34.64549796008729	37.0	34.0	40.0	23.0	41.0
47	34.48305607387963	37.0	33.0	40.0	23.0	41.0
48	34.396217464182925	37.0	33.0	40.0	23.0	41.0
49	34.248900977260504	37.0	33.0	40.0	23.0	41.0
50	34.05724406211455	36.0	33.0	40.0	22.0	41.0
51	31.813822385274676	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	11.0
11	8.0
12	3.0
13	9.0
14	5.0
15	12.0
16	21.0
17	26.0
18	60.0
19	85.0
20	198.0
21	307.0
22	417.0
23	637.0
24	897.0
25	1293.0
26	1455.0
27	1778.0
28	2082.0
29	2282.0
30	2825.0
31	3488.0
32	4455.0
33	6089.0
34	9769.0
35	11880.0
36	12210.0
37	18813.0
38	22315.0
39	23027.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62645877478732	19.544419494607673	23.866978715329388	12.962143015275625
2	31.360099939909546	25.454631708782692	25.36607735855024	17.81919099275752
3	31.80287169107182	24.68531579113824	27.39017679243493	16.121635725355006
4	28.12549416490085	24.37142224611784	28.297068218476234	19.206015370505074
5	26.455612131945983	29.136753218001832	25.414307852873275	18.99332679717891
6	26.098232075650717	35.230399443372654	23.85432809386761	14.817040387109017
7	60.63838198551503	28.585660520573075	6.837660900091717	3.9382965938201715
8	82.61409279230843	5.99481324520067	7.457541351718904	3.9335526107720042
9	76.68174199057529	7.365033682279643	9.59233372339416	6.360890603750908
10	43.67073594990354	24.282867895885385	16.54701287200734	15.499383282203738
11	33.48461368164711	24.354027641607896	23.52462127201999	18.636737404725007
12	30.213637369935796	22.3789493658876	28.199816565988804	19.2075966981878
13	23.916790537335146	25.76615326227901	30.698314304690218	19.618741895695628
14	21.006356937284547	27.008286157057466	30.216800025301243	21.768556880356748
15	19.8780796356621	24.380119548372814	35.92064897688098	19.821151839084095
16	23.7444258199184	22.73316676681742	32.30494323033619	21.217464182927987
17	23.443973560201144	22.98776052373573	28.927227300041114	24.641038616022012
18	24.682943799614158	22.983807204528922	29.92504506783896	22.408203928017965
19	24.388816850627787	26.005724406211456	27.213858755811376	22.391599987349377
20	26.942661058224488	25.304405578924065	27.625003953319204	20.127929409532243
21	25.81754641196749	26.096650747967992	27.885132357127045	20.200670482937475
22	24.418071412758152	23.84088680856447	27.617097314905592	24.12394446377178
23	23.49220405452418	25.901356779151776	27.17353489990196	23.43290426642209
24	24.276542585154495	23.79660963344824	28.946993896075146	22.979853885322115
25	22.908694139599607	26.551282456750684	27.02489009772605	23.51513330592365
26	22.18207406938866	26.367848445554888	28.19665391062336	23.253423574433093
27	22.618520509820044	25.691830861191058	28.722445365128564	22.967203263860338
28	20.25127296878459	26.84540940573706	30.83905246845251	22.06426515702584
29	23.242354280654038	25.32733483032354	29.105126664347384	22.325184224675038
30	24.537461652803692	24.562762895727253	29.30516461621177	21.59461083525728
31	26.046048262120873	25.075903728770676	26.603466270280528	22.27458173882792
32	25.850754293304657	25.872892880862775	26.975078275720293	21.30127455011227
33	25.78117587526487	25.05771846041937	26.370220437078974	22.79088522723679
34	23.163287896517918	24.95335083335969	29.901325152598123	21.982036117524274
35	23.840096144723113	25.207153926436636	28.29548689079351	22.657263038046743
36	25.553464688952843	26.758436383187323	27.379898162497234	20.3082007653626
37	24.146083051329896	26.700717922767957	29.009456339542677	20.143742686359467
38	23.0881748315886	26.863594674088365	27.36408488567001	22.684145608653026
39	24.31844776874664	24.909864322084825	28.657610930136947	22.114076979031594
40	25.672064265157026	23.509598659034125	28.236187102691417	22.58214997311743
41	21.405642177171956	24.525601695183276	28.438597046079888	25.63015908156488
42	23.50485467598596	23.837724153199026	28.678958853853697	23.97846231696132
43	24.737499604668077	23.84167747240583	27.912014927733324	23.50880799519276
44	22.70470286852842	25.088554350232457	28.523988740946898	23.682754040292227
45	23.02729371580379	25.236408488567	27.120560422530758	24.615737373098455
46	24.014832853663936	26.008887061576903	27.499288402542778	22.476991682216386
47	21.775672854929	25.96302855877795	29.760586988835826	22.500711597457226
48	23.9523704101964	24.569088206458144	29.282235364812298	22.19630601853316
49	23.90018659666656	23.368069831430468	29.57399032227458	23.15775324962839
50	21.28625193712641	25.247477782346056	29.870489262785032	23.595781017742496
51	22.652519054998578	23.741263164552958	27.768904772446945	25.837313008001516
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	100.0
1	202.0
2	304.0
3	458.0
4	612.0
5	437.0
6	262.0
7	264.0
8	266.0
9	257.5
10	249.0
11	256.0
12	263.0
13	269.5
14	276.0
15	290.0
16	304.0
17	317.5
18	331.0
19	348.5
20	366.0
21	449.0
22	532.0
23	565.5
24	599.0
25	758.0
26	1033.5
27	1150.0
28	1277.5
29	1405.0
30	1634.0
31	1863.0
32	2170.0
33	2477.0
34	2890.0
35	3303.0
36	3625.0
37	3947.0
38	4175.5
39	4404.0
40	5004.0
41	5604.0
42	6324.0
43	7044.0
44	7847.5
45	8651.0
46	9609.0
47	10567.0
48	10525.0
49	10483.0
50	10388.5
51	10294.0
52	9637.0
53	8980.0
54	8296.0
55	7612.0
56	7093.0
57	6574.0
58	6207.5
59	5841.0
60	5751.0
61	5661.0
62	5232.0
63	4803.0
64	4314.0
65	3825.0
66	3188.5
67	2552.0
68	2014.0
69	1476.0
70	1293.0
71	1110.0
72	853.5
73	597.0
74	492.5
75	295.5
76	203.0
77	169.0
78	135.0
79	106.5
80	78.0
81	55.0
82	32.0
83	25.5
84	19.0
85	13.0
86	7.0
87	6.0
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	126476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.86441696448338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.47878157160085	32.77696954362883
2	10.486148966747608	10.038268129921882
3	6.111964583643061	8.776368639109396
4	4.204040504154484	8.048957905057087
5	2.818111237755422	6.744362566811095
6	2.1639658390736245	6.214617793099086
7	1.6304078497447843	5.462696479964578
8	1.153013859292664	4.415066890160979
9	0.8457637477906075	3.6433789809924413
>10	2.0863273700381586	12.465606122900788
>50	0.013215058559228242	0.44435307884499825
>100	0.00825941159951765	0.9693538695088396
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	328	0.25933773996647586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	319	0.252221765394225	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	215	0.16999272589265949	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.16524874284449223	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	155	0.12255289541098706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10990227394920775	0.0
2	0.0	0.0	0.0	0.5629526550491792	0.0
3	0.0	0.0	0.0	0.6973655080805845	0.0
4	0.0	0.0	0.0	0.9037287706758594	0.0
5	0.0	0.0	0.0	1.6366741516176981	0.0
6	0.0	0.0	0.0	1.9323824282867896	0.0
7	0.0	0.0	0.0	2.2802745184857205	0.0
8	0.0	0.0	0.0	2.9365255068155225	0.0
9	0.0	0.0	0.0	3.2116765236092224	0.0
10	0.0	0.0	0.0	4.286188684019103	0.0
11	0.0	0.0	0.0	5.302982384009614	0.0
12	0.0	0.0	0.0	6.429678357949334	0.0
13	0.0	0.0	0.0	6.7301306176665925	0.0
14	0.0	0.0	0.0	6.860590151491192	0.0
15	0.0	0.0	0.0	7.12783452987128	0.0
16	0.0	0.0	0.0	7.610930136942978	0.0
17	0.0	0.0	0.0	8.128024289193206	0.0
18	0.0	0.0	0.0	8.690976944242387	0.0
19	0.0	0.0	0.0	9.02779974066226	0.0
20	0.0	0.0	0.0	9.357506562509883	0.0
21	0.0	0.0	0.0	9.763117113128182	0.0
22	0.0	0.0	0.0	10.142635756981562	0.0
23	0.0	0.0	0.0	10.510294443214523	0.0
24	0.0	0.0	0.0	10.84237325658623	0.0
25	0.0	0.0	0.0	11.125430911793542	0.0
26	0.0	0.0	0.0	11.361839400360543	0.0
27	0.0	0.0	0.0	11.659919668553718	0.0
28	0.0	0.0	0.0	11.952465289857365	0.0
29	0.0	0.0	0.0	12.226034978968341	0.0
30	0.0	0.0	0.0	12.600809639773553	0.0
31	0.0	0.0	0.0	12.89968057180809	0.0
32	0.0	0.0	0.0	13.189854201587654	0.0
33	0.0	0.0	0.0	13.478446503684493	0.0
34	0.0	0.0	0.0	13.782852082608558	0.0
35	0.0	0.0	0.0	14.116512223662987	0.0
36	0.0	0.0	0.0	14.43198709636611	0.0
37	0.0	0.0	0.0	14.751415288276037	0.0
38	0.0	0.0	0.0	15.051076884151934	0.0
39	0.0	0.0	0.0	15.438502166418925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGTGA	45	3.783498E-10	45.000004	43
AACACGT	45	3.783498E-10	45.000004	41
TTTAGGC	20	7.0094684E-4	45.0	4
TGCTTAC	20	7.0094684E-4	45.0	12
CAACGAC	20	7.0094684E-4	45.0	12
TATGCGG	25	3.8710758E-5	45.0	2
ATGTACG	20	7.0094684E-4	45.0	1
GTATGCG	20	7.0094684E-4	45.0	1
CGGGCTC	25	3.8710758E-5	45.0	6
AAACCGC	20	7.0094684E-4	45.0	22
CATAATG	20	7.0094684E-4	45.0	19
CGATGGA	25	3.8710758E-5	45.0	10
GCAATCG	20	7.0094684E-4	45.0	21
CAATCGG	20	7.0094684E-4	45.0	22
ACGACGC	20	7.0094684E-4	45.0	14
TAGTAAG	20	7.0094684E-4	45.0	2
CTCGTAA	55	6.002665E-11	40.909092	33
TCGTAAC	55	6.002665E-11	40.909092	34
CATGGTC	50	1.0659278E-9	40.5	12
GACACGA	50	1.0659278E-9	40.5	25
>>END_MODULE