##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936579.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169210
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04326576443473	33.0	31.0	34.0	30.0	34.0
2	32.16710596300455	33.0	31.0	34.0	30.0	34.0
3	32.069570356361915	33.0	31.0	34.0	30.0	34.0
4	35.81029489982861	37.0	35.0	37.0	35.0	37.0
5	35.864617930382366	37.0	35.0	37.0	35.0	37.0
6	35.83615625554045	37.0	35.0	37.0	35.0	37.0
7	26.68274333668223	35.0	10.0	37.0	0.0	37.0
8	31.07328172093848	35.0	19.0	37.0	17.0	37.0
9	35.908705159269545	39.0	32.0	39.0	32.0	39.0
10	36.67647893150523	38.0	35.0	39.0	33.0	39.0
11	37.0641628745346	39.0	37.0	39.0	34.0	39.0
12	37.109822114532236	39.0	37.0	39.0	34.0	39.0
13	37.11216240174931	39.0	37.0	39.0	33.0	39.0
14	38.3219254181195	40.0	38.0	41.0	34.0	41.0
15	38.390804325985464	40.0	38.0	41.0	34.0	41.0
16	38.3225636782696	40.0	37.0	41.0	34.0	41.0
17	38.206896755510904	40.0	37.0	41.0	33.0	41.0
18	38.10957390225164	40.0	37.0	41.0	33.0	41.0
19	38.01300750546658	40.0	37.0	41.0	33.0	41.0
20	38.059866438153776	40.0	37.0	41.0	34.0	41.0
21	37.98740027185155	40.0	37.0	41.0	33.0	41.0
22	37.97451096270906	40.0	37.0	41.0	33.0	41.0
23	37.90748182731517	40.0	36.0	41.0	33.0	41.0
24	37.87186927486555	40.0	36.0	41.0	33.0	41.0
25	37.74713669404881	40.0	36.0	41.0	33.0	41.0
26	37.616984811772355	40.0	36.0	41.0	33.0	41.0
27	37.47022043614444	40.0	36.0	41.0	33.0	41.0
28	37.41333845517404	40.0	36.0	41.0	33.0	41.0
29	37.38076354825365	40.0	36.0	41.0	32.0	41.0
30	37.23178299154896	39.0	35.0	41.0	32.0	41.0
31	37.11928373027599	39.0	35.0	41.0	31.0	41.0
32	36.975285148631876	39.0	35.0	41.0	31.0	41.0
33	36.85558182140535	39.0	35.0	41.0	31.0	41.0
34	36.697417410318536	39.0	35.0	41.0	30.0	41.0
35	36.58209916671591	39.0	35.0	41.0	30.0	41.0
36	36.422628686247855	39.0	35.0	41.0	30.0	41.0
37	36.37048637787365	39.0	35.0	41.0	30.0	41.0
38	36.25053483836653	39.0	35.0	41.0	30.0	41.0
39	36.20402458483541	39.0	35.0	40.0	30.0	41.0
40	36.00029549081024	39.0	35.0	40.0	29.0	41.0
41	35.89563855564092	38.0	35.0	40.0	28.0	41.0
42	35.807062230364636	38.0	35.0	40.0	28.0	41.0
43	35.77852963772827	38.0	35.0	40.0	28.0	41.0
44	35.56412150582117	38.0	34.0	40.0	27.0	41.0
45	35.44255067667395	38.0	34.0	40.0	27.0	41.0
46	35.2763193664677	38.0	34.0	40.0	26.0	41.0
47	35.18215826487796	38.0	34.0	40.0	26.0	41.0
48	35.10669582175994	38.0	34.0	40.0	26.0	41.0
49	34.94397494237929	38.0	34.0	40.0	26.0	41.0
50	34.72475622008155	37.0	34.0	40.0	24.0	41.0
51	32.630447373086696	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	15.0
11	10.0
12	2.0
13	6.0
14	8.0
15	9.0
16	26.0
17	45.0
18	79.0
19	119.0
20	227.0
21	326.0
22	493.0
23	723.0
24	1073.0
25	1400.0
26	1727.0
27	2024.0
28	2308.0
29	2773.0
30	3321.0
31	4204.0
32	5445.0
33	7728.0
34	11967.0
35	14589.0
36	16427.0
37	25850.0
38	32130.0
39	34138.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.94480231664795	19.918444536374917	23.166479522486853	12.970273624490277
2	30.505880267123693	25.04875598368891	25.95532178949235	18.490041959695052
3	33.27640210389457	23.909343419419656	26.445245552863305	16.36900892382247
4	30.167247798593465	25.3590213344365	25.903315406890847	18.570415460079193
5	25.07121328526683	31.350983984398084	23.798829856391464	19.77897287394362
6	27.13492110395367	35.02097984752674	24.108504225518587	13.735594823001005
7	60.94320666627267	28.76662135807576	6.149163761007033	4.141008214644525
8	82.50458010755865	5.7744814136280365	7.770226345960641	3.9507121328526686
9	75.57472962590863	8.449264227882512	10.1477454051179	5.828260741090952
10	42.483304769221675	25.92459074522782	16.91862183086106	14.67348265468944
11	32.47207611843272	25.429348147272616	23.227941611015897	18.870634123278766
12	29.702145263282315	22.700786005555226	27.83582530583299	19.76124342532947
13	25.239052065480767	24.411086815200047	28.7867147331718	21.56314638614739
14	21.057266119023698	27.246616630222796	28.996513208439218	22.699604042314284
15	21.003486791560782	25.525087169789018	32.448436853613856	21.022989185036344
16	24.480822646415696	24.690621121683115	30.33863246853023	20.48992376337096
17	23.80710359907807	24.856095975415165	27.555109036108977	23.781691389397793
18	24.65043437149105	25.503811831452044	27.590567933337272	22.255185863719635
19	26.327640210389458	26.315820577980027	25.533951894096095	21.822587317534424
20	27.63725548135453	25.334791087997168	26.598900774185925	20.429052656462385
21	26.90857514331304	26.097748360026003	26.13379823887477	20.859878257786182
22	25.234915194137464	25.367295077123103	26.13261627563383	23.265173453105607
23	24.961881685479582	25.702381655930502	27.27321080314402	22.062525855445898
24	25.237870102239818	23.917026180485788	28.02848531410673	22.816618403167663
25	24.36085337745996	25.718338159683235	26.311683706636725	23.609124756220083
26	23.13397553336091	26.677501329708647	26.832929495892678	23.355593641037764
27	23.344955971869275	26.970037231842092	27.22179540216299	22.463211394125643
28	22.438981147686306	27.54388038532002	27.72294781632291	22.294190650670764
29	24.32775840671355	26.270314993203712	26.90502925359021	22.496897346492524
30	24.450091602151172	25.659239997636075	27.862419478754212	22.028248921458545
31	25.42284734944743	25.354884463093196	26.46533892795934	22.75692925950003
32	26.220081555463626	26.59239997636074	26.19998818036759	20.98753028780805
33	25.673423556527393	27.046273860882923	25.626145026889663	21.654157555700017
34	23.89220495242598	26.93753324271615	27.109508894273386	22.06075291058448
35	23.861473908161457	26.296318184504464	27.23952485077714	22.60268305655694
36	24.293481472726196	29.461615743750368	25.417528514863186	20.827374268660247
37	24.882099166715914	28.653152886945215	25.97127829324508	20.493469653093786
38	24.163465516222445	28.232373973169434	25.01211512321967	22.592045387388453
39	24.678210507653212	26.749010105785707	25.6462384019857	22.92654098457538
40	24.502097984752673	25.86194669345783	27.12605637964659	22.5098989421429
41	21.707345901542464	27.64848413214349	26.95585367295077	23.688316293363275
42	23.17416228355298	27.731812540629985	26.87311624608475	22.220908929732285
43	24.490278352343243	25.892086756101882	26.548667336445835	23.068967555109037
44	23.281720938478813	26.55398617103008	26.780923113291177	23.383369777199928
45	23.95721293067786	26.08592872761657	25.990780686720644	23.96607765498493
46	22.797116009692097	27.543289403699546	26.301046037468232	23.35854854914012
47	22.817209384788136	26.930441463270494	27.763134566514978	22.489214585426392
48	22.677737722356834	25.97009633000414	28.073990898883043	23.278175048755983
49	22.7013769871757	26.29986407422729	27.634300573252172	23.364458365344838
50	21.15005023343774	26.496660953844337	27.90437917380769	24.44890963891023
51	21.72448436853614	25.503811831452044	27.60829738195142	25.163406418060397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	70.0
1	252.0
2	434.0
3	650.5
4	867.0
5	642.0
6	417.0
7	372.0
8	327.0
9	321.0
10	315.0
11	338.0
12	361.0
13	375.5
14	390.0
15	403.0
16	416.0
17	410.5
18	405.0
19	440.0
20	475.0
21	523.5
22	572.0
23	567.5
24	563.0
25	725.5
26	1128.5
27	1369.0
28	1814.0
29	2259.0
30	2625.0
31	2991.0
32	3517.0
33	4043.0
34	4555.5
35	5068.0
36	5195.0
37	5322.0
38	5895.0
39	6468.0
40	7017.0
41	7566.0
42	8280.0
43	8994.0
44	9685.5
45	10377.0
46	11235.5
47	12094.0
48	12364.5
49	12635.0
50	12564.0
51	12493.0
52	11907.5
53	11322.0
54	10895.0
55	10468.0
56	10098.0
57	9728.0
58	9466.0
59	9204.0
60	8902.5
61	8601.0
62	7938.5
63	7276.0
64	6218.0
65	5160.0
66	4257.5
67	3355.0
68	2843.5
69	2332.0
70	1918.0
71	1504.0
72	1239.5
73	975.0
74	728.0
75	388.0
76	295.0
77	215.5
78	136.0
79	109.0
80	82.0
81	60.0
82	38.0
83	35.5
84	33.0
85	24.5
86	16.0
87	14.0
88	12.0
89	8.0
90	4.0
91	4.5
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	169210.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.57142012883399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13535936927026	34.62265823532888
2	12.671892189218923	13.07014951834998
3	7.029244591125779	10.875243779918446
4	4.572332233223323	9.432066662726788
5	2.9427942794279427	7.588204006855387
6	1.9492574257425743	6.031558418533184
7	1.3224239090575725	4.773949530169612
8	0.8594609460946095	3.5458897228296196
9	0.5248441510817748	2.436026239583949
>10	0.9671800513384671	6.415105490219254
>50	0.01948111477814448	0.6737190473376278
>100	0.00572973964063073	0.5354293481472726
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	346	0.20447964068317478	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	175	0.1034217835825306	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	173	0.10223982034158739	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06500797825187636	0.0
2	0.0	0.0	0.0	0.3019916080609893	0.0
3	0.0	0.0	0.0	0.40600437326399147	0.0
4	0.0	0.0	0.0	0.5649784291708528	0.0
5	0.0	0.0	0.0	1.0507653211985106	0.0
6	0.0	0.0	0.0	1.3846699367649666	0.0
7	0.0	0.0	0.0	1.670114059452751	0.0
8	0.0	0.0	0.0	2.2652325512676557	0.0
9	0.0	0.0	0.0	2.5122628686247856	0.0
10	0.0	0.0	0.0	3.064830683765735	0.0
11	0.0	0.0	0.0	3.6883162933632763	0.0
12	0.0	0.0	0.0	4.208971100998759	0.0
13	0.0	0.0	0.0	4.465457124283435	0.0
14	0.0	0.0	0.0	4.564151054902193	0.0
15	0.0	0.0	0.0	4.749719283730276	0.0
16	0.0	0.0	0.0	5.10135334791088	0.0
17	0.0	0.0	0.0	5.539861710300809	0.0
18	0.0	0.0	0.0	5.966550440281307	0.0
19	0.0	0.0	0.0	6.231310206252585	0.0
20	0.0	0.0	0.0	6.493115064121506	0.0
21	0.0	0.0	0.0	6.812245139176172	0.0
22	0.0	0.0	0.0	7.181017670350452	0.0
23	0.0	0.0	0.0	7.49719283730276	0.0
24	0.0	0.0	0.0	7.767862419478754	0.0
25	0.0	0.0	0.0	8.014301755215413	0.0
26	0.0	0.0	0.0	8.262514035813487	0.0
27	0.0	0.0	0.0	8.492996867797412	0.0
28	0.0	0.0	0.0	8.758938597009633	0.0
29	0.0	0.0	0.0	9.01601560191478	0.0
30	0.0	0.0	0.0	9.297913834879735	0.0
31	0.0	0.0	0.0	9.563855564091957	0.0
32	0.0	0.0	0.0	9.820341587376632	0.0
33	0.0	0.0	0.0	10.064416996631405	0.0
34	0.0	0.0	0.0	10.32799479936174	0.0
35	0.0	0.0	0.0	10.605165179362922	0.0
36	0.0	0.0	0.0	10.883517522605047	0.0
37	0.0	0.0	0.0	11.147686306955853	0.0
38	0.0	0.0	0.0	11.464452455528633	0.0
39	0.0	0.0	0.0	11.794220199751788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCT	20	7.016224E-4	45.0	14
ACCGACT	20	7.016224E-4	45.0	42
ATGGGCT	70	0.0	41.785713	5
TCGACAC	50	1.0695658E-9	40.5	34
AGGCGAT	80	0.0	39.375	7
CGGTAGT	35	6.21676E-6	38.571426	12
GTTGCGG	35	6.21676E-6	38.571426	2
TACGGGA	65	9.094947E-12	38.07692	4
TATGGGT	30	1.13581234E-4	37.499996	4
TACGGTT	30	1.13581234E-4	37.499996	33
CACGACC	30	1.13581234E-4	37.499996	27
TCTCGTA	30	1.13581234E-4	37.499996	28
CGAGACA	30	1.13581234E-4	37.499996	22
ATTGCGG	30	1.13581234E-4	37.499996	2
ACGGTTA	30	1.13581234E-4	37.499996	34
GACTAAT	30	1.13581234E-4	37.499996	9
AACACGT	30	1.13581234E-4	37.499996	41
TAATGTC	55	2.7193892E-9	36.81818	25
TGTCCAT	55	2.7193892E-9	36.81818	28
GGCGATA	80	0.0	36.5625	8
>>END_MODULE