##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936579.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169210 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.04326576443473 33.0 31.0 34.0 30.0 34.0 2 32.16710596300455 33.0 31.0 34.0 30.0 34.0 3 32.069570356361915 33.0 31.0 34.0 30.0 34.0 4 35.81029489982861 37.0 35.0 37.0 35.0 37.0 5 35.864617930382366 37.0 35.0 37.0 35.0 37.0 6 35.83615625554045 37.0 35.0 37.0 35.0 37.0 7 26.68274333668223 35.0 10.0 37.0 0.0 37.0 8 31.07328172093848 35.0 19.0 37.0 17.0 37.0 9 35.908705159269545 39.0 32.0 39.0 32.0 39.0 10 36.67647893150523 38.0 35.0 39.0 33.0 39.0 11 37.0641628745346 39.0 37.0 39.0 34.0 39.0 12 37.109822114532236 39.0 37.0 39.0 34.0 39.0 13 37.11216240174931 39.0 37.0 39.0 33.0 39.0 14 38.3219254181195 40.0 38.0 41.0 34.0 41.0 15 38.390804325985464 40.0 38.0 41.0 34.0 41.0 16 38.3225636782696 40.0 37.0 41.0 34.0 41.0 17 38.206896755510904 40.0 37.0 41.0 33.0 41.0 18 38.10957390225164 40.0 37.0 41.0 33.0 41.0 19 38.01300750546658 40.0 37.0 41.0 33.0 41.0 20 38.059866438153776 40.0 37.0 41.0 34.0 41.0 21 37.98740027185155 40.0 37.0 41.0 33.0 41.0 22 37.97451096270906 40.0 37.0 41.0 33.0 41.0 23 37.90748182731517 40.0 36.0 41.0 33.0 41.0 24 37.87186927486555 40.0 36.0 41.0 33.0 41.0 25 37.74713669404881 40.0 36.0 41.0 33.0 41.0 26 37.616984811772355 40.0 36.0 41.0 33.0 41.0 27 37.47022043614444 40.0 36.0 41.0 33.0 41.0 28 37.41333845517404 40.0 36.0 41.0 33.0 41.0 29 37.38076354825365 40.0 36.0 41.0 32.0 41.0 30 37.23178299154896 39.0 35.0 41.0 32.0 41.0 31 37.11928373027599 39.0 35.0 41.0 31.0 41.0 32 36.975285148631876 39.0 35.0 41.0 31.0 41.0 33 36.85558182140535 39.0 35.0 41.0 31.0 41.0 34 36.697417410318536 39.0 35.0 41.0 30.0 41.0 35 36.58209916671591 39.0 35.0 41.0 30.0 41.0 36 36.422628686247855 39.0 35.0 41.0 30.0 41.0 37 36.37048637787365 39.0 35.0 41.0 30.0 41.0 38 36.25053483836653 39.0 35.0 41.0 30.0 41.0 39 36.20402458483541 39.0 35.0 40.0 30.0 41.0 40 36.00029549081024 39.0 35.0 40.0 29.0 41.0 41 35.89563855564092 38.0 35.0 40.0 28.0 41.0 42 35.807062230364636 38.0 35.0 40.0 28.0 41.0 43 35.77852963772827 38.0 35.0 40.0 28.0 41.0 44 35.56412150582117 38.0 34.0 40.0 27.0 41.0 45 35.44255067667395 38.0 34.0 40.0 27.0 41.0 46 35.2763193664677 38.0 34.0 40.0 26.0 41.0 47 35.18215826487796 38.0 34.0 40.0 26.0 41.0 48 35.10669582175994 38.0 34.0 40.0 26.0 41.0 49 34.94397494237929 38.0 34.0 40.0 26.0 41.0 50 34.72475622008155 37.0 34.0 40.0 24.0 41.0 51 32.630447373086696 35.0 31.0 39.0 19.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 12.0 10 15.0 11 10.0 12 2.0 13 6.0 14 8.0 15 9.0 16 26.0 17 45.0 18 79.0 19 119.0 20 227.0 21 326.0 22 493.0 23 723.0 24 1073.0 25 1400.0 26 1727.0 27 2024.0 28 2308.0 29 2773.0 30 3321.0 31 4204.0 32 5445.0 33 7728.0 34 11967.0 35 14589.0 36 16427.0 37 25850.0 38 32130.0 39 34138.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94480231664795 19.918444536374917 23.166479522486853 12.970273624490277 2 30.505880267123693 25.04875598368891 25.95532178949235 18.490041959695052 3 33.27640210389457 23.909343419419656 26.445245552863305 16.36900892382247 4 30.167247798593465 25.3590213344365 25.903315406890847 18.570415460079193 5 25.07121328526683 31.350983984398084 23.798829856391464 19.77897287394362 6 27.13492110395367 35.02097984752674 24.108504225518587 13.735594823001005 7 60.94320666627267 28.76662135807576 6.149163761007033 4.141008214644525 8 82.50458010755865 5.7744814136280365 7.770226345960641 3.9507121328526686 9 75.57472962590863 8.449264227882512 10.1477454051179 5.828260741090952 10 42.483304769221675 25.92459074522782 16.91862183086106 14.67348265468944 11 32.47207611843272 25.429348147272616 23.227941611015897 18.870634123278766 12 29.702145263282315 22.700786005555226 27.83582530583299 19.76124342532947 13 25.239052065480767 24.411086815200047 28.7867147331718 21.56314638614739 14 21.057266119023698 27.246616630222796 28.996513208439218 22.699604042314284 15 21.003486791560782 25.525087169789018 32.448436853613856 21.022989185036344 16 24.480822646415696 24.690621121683115 30.33863246853023 20.48992376337096 17 23.80710359907807 24.856095975415165 27.555109036108977 23.781691389397793 18 24.65043437149105 25.503811831452044 27.590567933337272 22.255185863719635 19 26.327640210389458 26.315820577980027 25.533951894096095 21.822587317534424 20 27.63725548135453 25.334791087997168 26.598900774185925 20.429052656462385 21 26.90857514331304 26.097748360026003 26.13379823887477 20.859878257786182 22 25.234915194137464 25.367295077123103 26.13261627563383 23.265173453105607 23 24.961881685479582 25.702381655930502 27.27321080314402 22.062525855445898 24 25.237870102239818 23.917026180485788 28.02848531410673 22.816618403167663 25 24.36085337745996 25.718338159683235 26.311683706636725 23.609124756220083 26 23.13397553336091 26.677501329708647 26.832929495892678 23.355593641037764 27 23.344955971869275 26.970037231842092 27.22179540216299 22.463211394125643 28 22.438981147686306 27.54388038532002 27.72294781632291 22.294190650670764 29 24.32775840671355 26.270314993203712 26.90502925359021 22.496897346492524 30 24.450091602151172 25.659239997636075 27.862419478754212 22.028248921458545 31 25.42284734944743 25.354884463093196 26.46533892795934 22.75692925950003 32 26.220081555463626 26.59239997636074 26.19998818036759 20.98753028780805 33 25.673423556527393 27.046273860882923 25.626145026889663 21.654157555700017 34 23.89220495242598 26.93753324271615 27.109508894273386 22.06075291058448 35 23.861473908161457 26.296318184504464 27.23952485077714 22.60268305655694 36 24.293481472726196 29.461615743750368 25.417528514863186 20.827374268660247 37 24.882099166715914 28.653152886945215 25.97127829324508 20.493469653093786 38 24.163465516222445 28.232373973169434 25.01211512321967 22.592045387388453 39 24.678210507653212 26.749010105785707 25.6462384019857 22.92654098457538 40 24.502097984752673 25.86194669345783 27.12605637964659 22.5098989421429 41 21.707345901542464 27.64848413214349 26.95585367295077 23.688316293363275 42 23.17416228355298 27.731812540629985 26.87311624608475 22.220908929732285 43 24.490278352343243 25.892086756101882 26.548667336445835 23.068967555109037 44 23.281720938478813 26.55398617103008 26.780923113291177 23.383369777199928 45 23.95721293067786 26.08592872761657 25.990780686720644 23.96607765498493 46 22.797116009692097 27.543289403699546 26.301046037468232 23.35854854914012 47 22.817209384788136 26.930441463270494 27.763134566514978 22.489214585426392 48 22.677737722356834 25.97009633000414 28.073990898883043 23.278175048755983 49 22.7013769871757 26.29986407422729 27.634300573252172 23.364458365344838 50 21.15005023343774 26.496660953844337 27.90437917380769 24.44890963891023 51 21.72448436853614 25.503811831452044 27.60829738195142 25.163406418060397 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 252.0 2 434.0 3 650.5 4 867.0 5 642.0 6 417.0 7 372.0 8 327.0 9 321.0 10 315.0 11 338.0 12 361.0 13 375.5 14 390.0 15 403.0 16 416.0 17 410.5 18 405.0 19 440.0 20 475.0 21 523.5 22 572.0 23 567.5 24 563.0 25 725.5 26 1128.5 27 1369.0 28 1814.0 29 2259.0 30 2625.0 31 2991.0 32 3517.0 33 4043.0 34 4555.5 35 5068.0 36 5195.0 37 5322.0 38 5895.0 39 6468.0 40 7017.0 41 7566.0 42 8280.0 43 8994.0 44 9685.5 45 10377.0 46 11235.5 47 12094.0 48 12364.5 49 12635.0 50 12564.0 51 12493.0 52 11907.5 53 11322.0 54 10895.0 55 10468.0 56 10098.0 57 9728.0 58 9466.0 59 9204.0 60 8902.5 61 8601.0 62 7938.5 63 7276.0 64 6218.0 65 5160.0 66 4257.5 67 3355.0 68 2843.5 69 2332.0 70 1918.0 71 1504.0 72 1239.5 73 975.0 74 728.0 75 388.0 76 295.0 77 215.5 78 136.0 79 109.0 80 82.0 81 60.0 82 38.0 83 35.5 84 33.0 85 24.5 86 16.0 87 14.0 88 12.0 89 8.0 90 4.0 91 4.5 92 5.0 93 4.5 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 169210.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.57142012883399 #Duplication Level Percentage of deduplicated Percentage of total 1 67.13535936927026 34.62265823532888 2 12.671892189218923 13.07014951834998 3 7.029244591125779 10.875243779918446 4 4.572332233223323 9.432066662726788 5 2.9427942794279427 7.588204006855387 6 1.9492574257425743 6.031558418533184 7 1.3224239090575725 4.773949530169612 8 0.8594609460946095 3.5458897228296196 9 0.5248441510817748 2.436026239583949 >10 0.9671800513384671 6.415105490219254 >50 0.01948111477814448 0.6737190473376278 >100 0.00572973964063073 0.5354293481472726 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 346 0.20447964068317478 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 175 0.1034217835825306 No Hit GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC 173 0.10223982034158739 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06500797825187636 0.0 2 0.0 0.0 0.0 0.3019916080609893 0.0 3 0.0 0.0 0.0 0.40600437326399147 0.0 4 0.0 0.0 0.0 0.5649784291708528 0.0 5 0.0 0.0 0.0 1.0507653211985106 0.0 6 0.0 0.0 0.0 1.3846699367649666 0.0 7 0.0 0.0 0.0 1.670114059452751 0.0 8 0.0 0.0 0.0 2.2652325512676557 0.0 9 0.0 0.0 0.0 2.5122628686247856 0.0 10 0.0 0.0 0.0 3.064830683765735 0.0 11 0.0 0.0 0.0 3.6883162933632763 0.0 12 0.0 0.0 0.0 4.208971100998759 0.0 13 0.0 0.0 0.0 4.465457124283435 0.0 14 0.0 0.0 0.0 4.564151054902193 0.0 15 0.0 0.0 0.0 4.749719283730276 0.0 16 0.0 0.0 0.0 5.10135334791088 0.0 17 0.0 0.0 0.0 5.539861710300809 0.0 18 0.0 0.0 0.0 5.966550440281307 0.0 19 0.0 0.0 0.0 6.231310206252585 0.0 20 0.0 0.0 0.0 6.493115064121506 0.0 21 0.0 0.0 0.0 6.812245139176172 0.0 22 0.0 0.0 0.0 7.181017670350452 0.0 23 0.0 0.0 0.0 7.49719283730276 0.0 24 0.0 0.0 0.0 7.767862419478754 0.0 25 0.0 0.0 0.0 8.014301755215413 0.0 26 0.0 0.0 0.0 8.262514035813487 0.0 27 0.0 0.0 0.0 8.492996867797412 0.0 28 0.0 0.0 0.0 8.758938597009633 0.0 29 0.0 0.0 0.0 9.01601560191478 0.0 30 0.0 0.0 0.0 9.297913834879735 0.0 31 0.0 0.0 0.0 9.563855564091957 0.0 32 0.0 0.0 0.0 9.820341587376632 0.0 33 0.0 0.0 0.0 10.064416996631405 0.0 34 0.0 0.0 0.0 10.32799479936174 0.0 35 0.0 0.0 0.0 10.605165179362922 0.0 36 0.0 0.0 0.0 10.883517522605047 0.0 37 0.0 0.0 0.0 11.147686306955853 0.0 38 0.0 0.0 0.0 11.464452455528633 0.0 39 0.0 0.0 0.0 11.794220199751788 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTCT 20 7.016224E-4 45.0 14 ACCGACT 20 7.016224E-4 45.0 42 ATGGGCT 70 0.0 41.785713 5 TCGACAC 50 1.0695658E-9 40.5 34 AGGCGAT 80 0.0 39.375 7 CGGTAGT 35 6.21676E-6 38.571426 12 GTTGCGG 35 6.21676E-6 38.571426 2 TACGGGA 65 9.094947E-12 38.07692 4 TATGGGT 30 1.13581234E-4 37.499996 4 TACGGTT 30 1.13581234E-4 37.499996 33 CACGACC 30 1.13581234E-4 37.499996 27 TCTCGTA 30 1.13581234E-4 37.499996 28 CGAGACA 30 1.13581234E-4 37.499996 22 ATTGCGG 30 1.13581234E-4 37.499996 2 ACGGTTA 30 1.13581234E-4 37.499996 34 GACTAAT 30 1.13581234E-4 37.499996 9 AACACGT 30 1.13581234E-4 37.499996 41 TAATGTC 55 2.7193892E-9 36.81818 25 TGTCCAT 55 2.7193892E-9 36.81818 28 GGCGATA 80 0.0 36.5625 8 >>END_MODULE