##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936577.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	207373
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09271698822894	33.0	31.0	34.0	30.0	34.0
2	32.22009615523718	33.0	31.0	34.0	30.0	34.0
3	31.92366894436595	33.0	31.0	34.0	30.0	34.0
4	35.750020494471315	37.0	35.0	37.0	35.0	37.0
5	35.894586084012865	37.0	35.0	37.0	35.0	37.0
6	35.891634880143506	37.0	35.0	37.0	35.0	37.0
7	26.849560936091006	36.0	10.0	37.0	0.0	37.0
8	31.241502027747103	37.0	19.0	37.0	17.0	37.0
9	36.10810954174362	39.0	32.0	39.0	32.0	39.0
10	36.78911430128319	38.0	35.0	39.0	33.0	39.0
11	37.139786761053756	39.0	37.0	39.0	34.0	39.0
12	37.27683931852266	39.0	37.0	39.0	34.0	39.0
13	37.32671080613195	39.0	37.0	39.0	34.0	39.0
14	38.601158299296436	40.0	38.0	41.0	35.0	41.0
15	38.6800692472019	40.0	38.0	41.0	35.0	41.0
16	38.58948368398972	40.0	38.0	41.0	34.0	41.0
17	38.496867962560216	40.0	38.0	41.0	34.0	41.0
18	38.35966109377788	40.0	38.0	41.0	34.0	41.0
19	38.2018874202524	40.0	37.0	41.0	34.0	41.0
20	38.21261205653581	40.0	37.0	41.0	34.0	41.0
21	38.161236033620575	40.0	37.0	41.0	34.0	41.0
22	38.15197253258621	40.0	37.0	41.0	34.0	41.0
23	38.07280118433933	40.0	37.0	41.0	34.0	41.0
24	38.0216952062226	40.0	36.0	41.0	34.0	41.0
25	37.93370400196747	40.0	36.0	41.0	33.0	41.0
26	37.83233593572934	40.0	36.0	41.0	33.0	41.0
27	37.740192792697215	40.0	36.0	41.0	33.0	41.0
28	37.72534997323663	40.0	36.0	41.0	33.0	41.0
29	37.70104594137135	40.0	36.0	41.0	33.0	41.0
30	37.61235551397723	40.0	36.0	41.0	33.0	41.0
31	37.48377078983281	40.0	36.0	41.0	33.0	41.0
32	37.29464780853824	40.0	36.0	41.0	32.0	41.0
33	37.133353908175124	40.0	36.0	41.0	32.0	41.0
34	36.955235252419556	40.0	35.0	41.0	31.0	41.0
35	36.79630424404334	40.0	35.0	41.0	31.0	41.0
36	36.61503185081954	40.0	35.0	41.0	30.0	41.0
37	36.53940001832447	39.0	35.0	41.0	30.0	41.0
38	36.41013535995525	39.0	35.0	41.0	30.0	41.0
39	36.38199283416838	39.0	35.0	41.0	30.0	41.0
40	36.247004190516606	39.0	35.0	41.0	29.0	41.0
41	36.17272258201405	39.0	35.0	41.0	29.0	41.0
42	36.06949795778621	39.0	35.0	41.0	29.0	41.0
43	36.039764096579596	39.0	35.0	41.0	28.0	41.0
44	35.856355456110485	39.0	35.0	41.0	27.0	41.0
45	35.751023518008616	39.0	35.0	40.0	27.0	41.0
46	35.64602913590487	39.0	35.0	40.0	27.0	41.0
47	35.552559880022955	39.0	35.0	40.0	26.0	41.0
48	35.476826780728445	38.0	35.0	40.0	26.0	41.0
49	35.367617770876635	38.0	35.0	40.0	26.0	41.0
50	35.18747377913229	38.0	34.0	40.0	25.0	41.0
51	33.32764631846962	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	14.0
10	18.0
11	14.0
12	5.0
13	7.0
14	10.0
15	17.0
16	19.0
17	40.0
18	81.0
19	135.0
20	210.0
21	339.0
22	528.0
23	851.0
24	1365.0
25	1980.0
26	2538.0
27	2754.0
28	2888.0
29	3083.0
30	3586.0
31	4371.0
32	5460.0
33	7893.0
34	12271.0
35	15910.0
36	18291.0
37	31140.0
38	41914.0
39	49629.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97736928143972	19.643347976834015	24.155989448963943	15.223293292762316
2	34.24457378733008	23.332352813529244	26.04244525565045	16.380628143490235
3	31.37390113467038	22.96104121558737	29.777261263520323	15.887796386221927
4	27.707078549280766	25.224113071614916	28.91649346829144	18.152314910812883
5	24.717296851566985	29.760865686468346	27.22823125479209	18.293606207172584
6	26.050160821321967	34.48761410598294	26.558423709933308	12.903801362761785
7	61.1704513123695	27.908165479594736	7.696758980195108	3.2246242278406543
8	83.96753675743707	4.421501352635107	8.842520482415743	2.768441407512068
9	77.92673105949184	7.193800542982934	10.646516180987883	4.232952216537351
10	38.07052991469478	30.23103296957656	18.44598862918509	13.252448486543573
11	27.953494427914915	26.58832152691045	28.385083882665533	17.073100162509103
12	25.13297295212009	25.507177887188785	30.441764356979935	18.91808480371119
13	22.71510755980769	26.330814522623484	31.745694955466718	19.208382962102107
14	20.261075453410037	28.143490232576085	31.05418738215679	20.54124693185709
15	20.51520689771571	27.170364512255695	33.90557112063769	18.408857469390906
16	24.230251768552318	26.330814522623484	31.535928013772285	17.90300569505191
17	24.390349756236347	25.67209810341751	30.073346096164883	19.864206044181255
18	24.445805384500392	25.15515520342571	31.03489846797799	19.364140944095904
19	24.167080574616755	26.943719770654813	29.626325510071222	19.262874144657214
20	24.76600135986845	26.73105949183356	29.814392423314512	18.688546724983485
21	24.251469574149	26.888264142390767	30.482753299609882	18.377512983850355
22	24.159365008945233	24.92995713038824	29.80571241193405	21.104965448732475
23	21.922333187059067	27.1414311409875	30.88155160025654	20.054684071696894
24	21.181156659738733	25.707782594648293	32.66963394463117	20.441426800981805
25	21.395263607123397	28.253919266249707	30.23633742097573	20.11447970565117
26	21.396228052832335	27.858978748438805	29.963399285345734	20.78139391338313
27	20.849869558717867	27.500204944713154	31.24611207823584	20.403813418333147
28	20.321835533073255	27.35987809406239	31.85130176059564	20.466984612268714
29	20.91979187261601	26.430634653498768	31.866250669084213	20.78332280480101
30	21.34029020171382	26.34383453969417	31.747623846884597	20.568251411707404
31	22.413718275763962	27.166506729419932	30.308188626291756	20.11158636852435
32	22.47206724115483	27.11973111253635	30.476002179647306	19.932199466661523
33	22.04964002063914	26.959633124852317	30.242124095229368	20.748602759279173
34	21.442521446861456	27.03775322727645	31.181494215736862	20.338231110125232
35	20.83395620452036	26.959150901997848	31.35364777478264	20.853245118699157
36	22.01250886084495	27.945778862243397	29.718912298129457	20.322799978782193
37	21.63492836579497	27.80834534871946	30.05405718198608	20.50266910349949
38	21.06252983753912	28.364830522777794	29.40884300270527	21.163796636977814
39	21.970555472506064	27.452947104975095	29.231384992260324	21.34511243025852
40	21.617568343034048	26.737328388941666	30.665515761454	20.979587506570287
41	20.988749740805215	27.22630236337421	29.96966818245384	21.815279713366735
42	21.33498575031465	27.312138031469864	29.603661035911138	21.749215182304347
43	21.83697974181788	27.386882573912708	29.216918306626223	21.559219377643185
44	21.49701262941656	27.74854971476518	29.12288485000458	21.631552805813676
45	21.79165079349771	27.36807588258838	29.02210027342036	21.818173050493556
46	21.161385522705466	27.75915861756352	29.438258596827936	21.641197262903077
47	20.972836386607707	27.829563154316133	30.245017432356192	20.952583026719967
48	21.118949911512104	27.03582433585857	30.372806488790733	21.472419263838592
49	21.7612707536661	26.592179309746207	30.243088540938306	21.403461395649384
50	20.304957733166805	27.76301640039928	29.79317461771783	22.138851248716083
51	20.314602190256206	26.64715271515578	29.770992366412212	23.267252728175798
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	219.0
2	373.0
3	1690.5
4	3008.0
5	2152.5
6	1297.0
7	1075.0
8	853.0
9	895.5
10	938.0
11	839.0
12	740.0
13	744.5
14	749.0
15	738.0
16	727.0
17	758.0
18	789.0
19	788.5
20	788.0
21	850.5
22	913.0
23	1069.5
24	1226.0
25	1485.5
26	2042.5
27	2340.0
28	2792.0
29	3244.0
30	3886.0
31	4528.0
32	5398.0
33	6268.0
34	6975.0
35	7682.0
36	8267.0
37	8852.0
38	9548.5
39	10245.0
40	11234.5
41	12224.0
42	12687.5
43	13151.0
44	13786.0
45	14421.0
46	15328.5
47	16236.0
48	16428.0
49	16620.0
50	16155.5
51	15691.0
52	14546.5
53	13402.0
54	12395.0
55	11388.0
56	10385.5
57	9383.0
58	8556.5
59	7730.0
60	7017.0
61	6304.0
62	5493.5
63	4683.0
64	3883.0
65	3083.0
66	2564.0
67	2045.0
68	1688.0
69	1331.0
70	1092.0
71	853.0
72	729.5
73	606.0
74	483.0
75	279.5
76	199.0
77	165.0
78	131.0
79	105.0
80	79.0
81	57.5
82	36.0
83	27.5
84	19.0
85	14.5
86	10.0
87	8.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	3.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	207373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.81063853272536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.78574717772617	32.598549646299745
2	11.856955114639282	11.574911003988143
3	6.7753434276053515	9.92126516939764
4	4.4769922945842975	8.740994104190035
5	2.964989007771344	7.236150335591556
6	2.1539918522927266	6.30826306228175
7	1.4969943331878364	5.11484744979388
8	1.075995926744213	4.2016038594397305
9	0.7629971116194003	3.351813859509125
>10	1.641993784113835	9.84848525459514
>50	0.0039999848577681645	0.11812129809248254
>100	0.0029999886433261236	0.29091030439305615
>500	0.0	0.0
>1k	9.999962144420411E-4	0.6940846524277279
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1422	0.68572089905629	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	243	0.11718015363620143	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03472004552183746	0.0
2	0.0	0.0	0.0	0.1490068620312191	0.0
3	0.0	0.0	0.0	0.23484253012687284	0.0
4	0.0	0.0	0.0	0.33562710671109547	0.0
5	0.0	0.0	0.0	0.6317119393556538	0.0
6	0.0	0.0	0.0	0.8916300579149649	0.0
7	0.0	0.0	0.0	1.0985036624825797	0.0
8	0.0	0.0	0.0	1.4992308545471205	0.0
9	0.0	0.0	0.0	1.7027288991334455	0.0
10	0.0	0.0	0.0	2.1261205653580744	0.0
11	0.0	0.0	0.0	2.5620500257989227	0.0
12	0.0	0.0	0.0	2.9208238295245765	0.0
13	0.0	0.0	0.0	3.089601828589064	0.0
14	0.0	0.0	0.0	3.1706152681400184	0.0
15	0.0	0.0	0.0	3.28393763894046	0.0
16	0.0	0.0	0.0	3.527942403302262	0.0
17	0.0	0.0	0.0	3.8327072473272796	0.0
18	0.0	0.0	0.0	4.155314336967686	0.0
19	0.0	0.0	0.0	4.37520795860599	0.0
20	0.0	0.0	0.0	4.581599340319135	0.0
21	0.0	0.0	0.0	4.826568550389877	0.0
22	0.0	0.0	0.0	5.0797355489866085	0.0
23	0.0	0.0	0.0	5.373891490213287	0.0
24	0.0	0.0	0.0	5.622718483119789	0.0
25	0.0	0.0	0.0	5.841647659049153	0.0
26	0.0	0.0	0.0	6.0297145722924395	0.0
27	0.0	0.0	0.0	6.231283725460885	0.0
28	0.0	0.0	0.0	6.451177347099188	0.0
29	0.0	0.0	0.0	6.663355403065973	0.0
30	0.0	0.0	0.0	6.954135784311362	0.0
31	0.0	0.0	0.0	7.213571680016202	0.0
32	0.0	0.0	0.0	7.448896432997546	0.0
33	0.0	0.0	0.0	7.715083448664966	0.0
34	0.0	0.0	0.0	7.960052658735708	0.0
35	0.0	0.0	0.0	8.237330800055938	0.0
36	0.0	0.0	0.0	8.476995558727511	0.0
37	0.0	0.0	0.0	8.721482545943783	0.0
38	0.0	0.0	0.0	8.969345093141344	0.0
39	0.0	0.0	0.0	9.254821022987564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTG	35	1.2054988E-7	45.000004	5
TTAATCG	35	1.2054988E-7	45.000004	20
CATGCGG	20	7.019902E-4	45.000004	2
TATGCGG	20	7.019902E-4	45.000004	2
CGGGAGT	20	7.019902E-4	45.000004	6
CGTAAAG	20	7.019902E-4	45.000004	2
ATACCGG	20	7.019902E-4	45.000004	2
TAATCGT	35	1.2054988E-7	45.000004	21
CGTTTTT	680	0.0	43.345592	1
CGTTTTA	105	0.0	42.857143	1
GCGATGC	45	1.915214E-8	40.0	9
CGTTGAT	40	3.4399636E-7	39.375004	25
ATCGTTG	40	3.4399636E-7	39.375004	23
GCCTATG	40	3.4399636E-7	39.375004	9
CTGGGCG	35	6.2235504E-6	38.57143	5
CGGTAGT	35	6.2235504E-6	38.57143	12
AGTGCGG	30	1.1366976E-4	37.500004	2
CGAGGCA	30	1.1366976E-4	37.500004	5
CAGCTCG	30	1.1366976E-4	37.500004	25
CGATAGC	30	1.1366976E-4	37.500004	10
>>END_MODULE