##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936576.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130231
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09878600333254	33.0	31.0	34.0	30.0	34.0
2	32.20894410700985	33.0	31.0	34.0	30.0	34.0
3	32.12943922721933	33.0	31.0	34.0	30.0	34.0
4	35.844453317566476	37.0	35.0	37.0	35.0	37.0
5	35.902557762744664	37.0	35.0	37.0	35.0	37.0
6	35.88277752608826	37.0	35.0	37.0	35.0	37.0
7	26.798803664258127	36.0	10.0	37.0	0.0	37.0
8	31.138354155308644	36.0	19.0	37.0	17.0	37.0
9	35.956715374987525	39.0	32.0	39.0	32.0	39.0
10	36.746005175419064	38.0	35.0	39.0	33.0	39.0
11	37.10097442237255	39.0	37.0	39.0	34.0	39.0
12	37.101005137025744	39.0	37.0	39.0	34.0	39.0
13	37.002864141410264	39.0	37.0	39.0	33.0	39.0
14	38.25571484515975	40.0	37.0	41.0	34.0	41.0
15	38.333453632391674	40.0	37.0	41.0	34.0	41.0
16	38.31591556541837	40.0	37.0	41.0	34.0	41.0
17	38.20608764426289	40.0	37.0	41.0	34.0	41.0
18	38.10292480285032	40.0	37.0	41.0	33.0	41.0
19	38.008039560473314	40.0	37.0	41.0	34.0	41.0
20	38.059033563437275	40.0	37.0	41.0	34.0	41.0
21	38.00711044221422	40.0	37.0	41.0	34.0	41.0
22	38.02300527524169	40.0	37.0	41.0	34.0	41.0
23	37.97966689958612	40.0	36.0	41.0	34.0	41.0
24	37.90757192987844	40.0	36.0	41.0	33.0	41.0
25	37.82578648708833	40.0	36.0	41.0	33.0	41.0
26	37.711673871812394	40.0	36.0	41.0	33.0	41.0
27	37.58069891193341	40.0	36.0	41.0	33.0	41.0
28	37.529413119764115	40.0	36.0	41.0	33.0	41.0
29	37.487917623300135	40.0	36.0	41.0	33.0	41.0
30	37.42575116523716	40.0	36.0	41.0	33.0	41.0
31	37.2739439918299	40.0	35.0	41.0	32.0	41.0
32	37.15110073638381	39.0	35.0	41.0	32.0	41.0
33	37.06824028073193	39.0	35.0	41.0	32.0	41.0
34	36.950672266971765	39.0	35.0	41.0	31.0	41.0
35	36.890225829487605	39.0	35.0	41.0	31.0	41.0
36	36.68542052199553	39.0	35.0	41.0	31.0	41.0
37	36.623422994525114	39.0	35.0	41.0	30.0	41.0
38	36.47513264890848	39.0	35.0	41.0	30.0	41.0
39	36.4408627746082	39.0	35.0	41.0	30.0	41.0
40	36.248466187006166	39.0	35.0	41.0	30.0	41.0
41	36.17259331495573	39.0	35.0	40.0	30.0	41.0
42	36.09561471539035	39.0	35.0	40.0	29.0	41.0
43	36.04341516228855	39.0	35.0	40.0	29.0	41.0
44	35.84610423017561	38.0	35.0	40.0	28.0	41.0
45	35.76271394675615	38.0	35.0	40.0	28.0	41.0
46	35.60084004576483	38.0	35.0	40.0	27.0	41.0
47	35.41074705715229	38.0	34.0	40.0	27.0	41.0
48	35.33576491004446	38.0	34.0	40.0	26.0	41.0
49	35.154686672144116	38.0	34.0	40.0	26.0	41.0
50	35.00869224685367	38.0	34.0	40.0	26.0	41.0
51	32.90896944659874	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	11.0
10	21.0
11	7.0
12	8.0
13	2.0
14	2.0
15	13.0
16	13.0
17	32.0
18	53.0
19	105.0
20	174.0
21	227.0
22	335.0
23	481.0
24	702.0
25	1011.0
26	1219.0
27	1505.0
28	1638.0
29	1972.0
30	2502.0
31	3116.0
32	3960.0
33	5669.0
34	9243.0
35	11500.0
36	11824.0
37	19346.0
38	25215.0
39	28307.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.72591011356743	19.61821686080887	23.9244112384916	11.731461787132096
2	30.713885326842306	27.200896867873244	24.818975512742742	17.266242292541715
3	33.810690235043886	25.185247752071323	25.243605593138348	15.760456419746449
4	30.336095092566286	25.12996137632361	26.576621541722016	17.957321989388085
5	26.81235650497961	30.84135113759397	23.34160069415116	19.00469166327526
6	27.065752393823285	36.379203108322905	23.015257503973707	13.539786993880105
7	60.798888129554406	28.83491641775	6.349486681358509	4.016708771337086
8	82.39282505701408	6.7802596923927485	7.101995684591227	3.7249195660019505
9	75.74617410601162	9.128394929010758	8.589352765470586	6.536078199507029
10	42.6158134391965	27.527240058050694	15.78195667698167	14.07498982577113
11	34.67377198977202	25.949274750251476	22.36180325728897	17.01515000268753
12	32.161313358570546	23.25636753154011	26.10284801621734	18.479471093672014
13	24.739117414440496	26.930607919773326	28.03863903371701	20.29163563206917
14	20.441369566385884	30.525758076034126	27.02735907733182	22.005513280248174
15	20.316975220953537	26.45529866160899	33.37300642704118	19.854719690396298
16	23.094347735946126	24.70917062757715	31.442590473850313	20.75389116262641
17	23.595764449324662	24.419685021231505	28.21294469058826	23.77160583885557
18	23.46369144059402	26.91832205849606	28.117729265689427	21.500257235220495
19	26.715605347421125	26.414601746128035	25.80107654859442	21.068716357856424
20	28.66752155784721	25.43326857660618	26.281760871067565	19.61744899447904
21	25.884006112215985	27.314541084687978	25.80184441492425	20.999608388171787
22	24.405863427294577	25.488554952353894	25.529251867834844	24.57632975251668
23	24.276094017553422	27.711527977209727	26.342422311124082	21.66995569411277
24	23.492102494797706	26.069829764034676	28.00562078153435	22.432446959633268
25	22.697360843424377	28.285891991922046	26.631140051139894	22.385607113513682
26	23.13734825041657	29.16202747425728	25.618324362095045	22.082299913231104
27	23.99275134184641	28.412589936343878	26.414601746128035	21.180056975681673
28	21.267593737282215	28.37342875352259	29.358601254693582	21.000376254501614
29	23.303207377659696	28.01560304382213	27.028126943661647	21.653062634856525
30	22.88164876258341	27.82747579301395	27.284594297824633	22.006281146578004
31	26.11590174382443	26.86994647971681	25.516966006557578	21.497185769901176
32	25.694343128748148	29.248028503198164	25.43480430926584	19.622824058787845
33	24.37591664043123	29.082937242284864	25.067764203607435	21.473381913676466
34	24.323701730002842	28.112354201380622	25.96923927482704	21.594704793789496
35	24.21389684483725	27.408220776927152	26.048329506799455	22.32955287143614
36	24.850458032265742	29.236510508250724	25.03167448610546	20.881356973378075
37	24.123288617917392	30.29923750873448	26.207277837074123	19.370196036274006
38	22.401732306440095	29.793213597376965	25.314249295482643	22.490804800700296
39	24.686134637682272	27.533382988689333	26.128187605101704	21.652294768526694
40	24.3958811650068	27.795225407161123	27.36598812878654	20.44290529904554
41	21.8227610937488	27.53722232033848	26.84767835615176	23.79233822976096
42	22.01242407721664	28.483233638688176	26.1228125407929	23.381529743302288
43	23.605746711612444	27.709224378220238	25.287373973938614	23.3976549362287
44	23.131973186107764	28.729718730563388	25.684360866460366	22.453947216868485
45	22.744200689543963	28.070121553240014	25.195230014359097	23.990447742856922
46	23.623407637198515	29.261082230805265	24.82895777503052	22.2865523569657
47	23.416083728144603	27.041948537598575	27.86740484216508	21.674562892091746
48	23.359261619737236	26.86994647971681	27.059609463184646	22.711182437361305
49	21.660741298154818	27.21087913016102	28.230605616174337	22.897773955509827
50	21.025715843385985	27.660848799440995	27.61016962167226	23.703265735500764
51	22.073085517273153	27.218557793459315	25.80107654859442	24.90728014067311
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	196.0
2	331.0
3	459.0
4	587.0
5	437.0
6	287.0
7	251.5
8	216.0
9	210.5
10	205.0
11	216.0
12	227.0
13	224.0
14	221.0
15	243.5
16	266.0
17	269.0
18	272.0
19	293.5
20	315.0
21	345.0
22	375.0
23	471.0
24	567.0
25	810.0
26	1294.5
27	1536.0
28	2018.0
29	2500.0
30	2605.0
31	2710.0
32	2920.5
33	3131.0
34	3396.0
35	3661.0
36	3962.5
37	4264.0
38	4728.0
39	5192.0
40	5754.0
41	6316.0
42	6903.5
43	7491.0
44	8331.5
45	9172.0
46	10008.0
47	10844.0
48	10086.5
49	9329.0
50	9130.0
51	8931.0
52	8578.5
53	8226.0
54	7836.0
55	7446.0
56	7214.0
57	6982.0
58	6923.5
59	6865.0
60	6607.0
61	6349.0
62	5519.5
63	4690.0
64	4024.5
65	3359.0
66	2790.0
67	2221.0
68	1880.0
69	1539.0
70	1285.0
71	1031.0
72	854.0
73	677.0
74	525.5
75	297.0
76	220.0
77	150.0
78	80.0
79	58.5
80	37.0
81	34.5
82	32.0
83	27.5
84	23.0
85	14.0
86	5.0
87	8.0
88	11.0
89	7.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	130231.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.5920095829718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.70036777962068	27.55718684491404
2	11.11992500180284	9.472399044774285
3	6.425326314271292	8.210026798534912
4	4.434989543520588	7.555804685520344
5	3.2883824908055095	7.0029409280432455
6	2.415807312324223	6.173645291827599
7	1.9254344847479627	5.740568681803872
8	1.5215980385086896	5.184633459007456
9	1.1195644335472705	4.2916049174159765
>10	3.0071392514603015	16.717217866713764
>50	0.027042619167808462	0.7256336816886916
>100	0.014422730222831182	1.3683377997558186
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	367	0.2818069430473543	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	364	0.2795033440578664	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	256	0.19657378043630166	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	226	0.17353779054142254	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	174	0.13360874139029877	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	167	0.12823367708149366	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05989357372668566	0.0
2	0.0	0.0	0.0	0.30791439826155065	0.0
3	0.0	0.0	0.0	0.38239743225499306	0.0
4	0.0	0.0	0.0	0.519077638964609	0.0
5	0.0	0.0	0.0	1.1164776435718071	0.0
6	0.0	0.0	0.0	1.3107478250186209	0.0
7	0.0	0.0	0.0	1.5564650505639979	0.0
8	0.0	0.0	0.0	1.9588270073945528	0.0
9	0.0	0.0	0.0	2.1077930753814376	0.0
10	0.0	0.0	0.0	2.907141924733742	0.0
11	0.0	0.0	0.0	3.3786118512489347	0.0
12	0.0	0.0	0.0	4.177960700601239	0.0
13	0.0	0.0	0.0	4.3630164860901015	0.0
14	0.0	0.0	0.0	4.46437484162757	0.0
15	0.0	0.0	0.0	4.654037825095408	0.0
16	0.0	0.0	0.0	4.941987698781396	0.0
17	0.0	0.0	0.0	5.270634487948338	0.0
18	0.0	0.0	0.0	5.638442459936575	0.0
19	0.0	0.0	0.0	5.857284363937926	0.0
20	0.0	0.0	0.0	6.070751203630472	0.0
21	0.0	0.0	0.0	6.34795094869885	0.0
22	0.0	0.0	0.0	6.622847094777741	0.0
23	0.0	0.0	0.0	6.900814706175949	0.0
24	0.0	0.0	0.0	7.119656610177301	0.0
25	0.0	0.0	0.0	7.330051984550529	0.0
26	0.0	0.0	0.0	7.532000829295637	0.0
27	0.0	0.0	0.0	7.705538619837059	0.0
28	0.0	0.0	0.0	7.928987721817386	0.0
29	0.0	0.0	0.0	8.137079497201126	0.0
30	0.0	0.0	0.0	8.395082584023774	0.0
31	0.0	0.0	0.0	8.639264076909491	0.0
32	7.678663298293033E-4	0.0	0.0	8.866552510538966	0.0
33	7.678663298293033E-4	0.0	0.0	9.096912409487757	0.0
34	7.678663298293033E-4	0.0	0.0	9.344165367692792	0.0
35	7.678663298293033E-4	0.0	0.0	9.596025523876804	0.0
36	7.678663298293033E-4	0.0	0.0	9.849421412720474	0.0
37	7.678663298293033E-4	0.0	0.0	10.098977969914998	0.0
38	7.678663298293033E-4	0.0	0.0	10.374641982323718	0.0
39	7.678663298293033E-4	0.0	0.0	10.655681059041243	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.0102385E-4	45.0	11
CTCACGT	20	7.0102385E-4	45.0	45
CTCACGA	20	7.0102385E-4	45.0	24
GGTACCA	20	7.0102385E-4	45.0	8
GCAAGAC	35	1.2008604E-7	45.0	45
ATCTATC	25	3.871714E-5	45.0	3
ATAGGGT	20	7.0102385E-4	45.0	4
CGACGGT	25	3.871714E-5	45.0	28
TTGCTAG	25	3.871714E-5	45.0	1
GGGTAAT	20	7.0102385E-4	45.0	7
GAATCTA	20	7.0102385E-4	45.0	1
CACGACG	20	7.0102385E-4	45.0	26
TATGCCA	25	3.871714E-5	45.0	10
AATCTAT	20	7.0102385E-4	45.0	2
GACGGTC	25	3.871714E-5	45.0	29
ATAAGGC	20	7.0102385E-4	45.0	4
ACTAACC	20	7.0102385E-4	45.0	14
TTACGGG	25	3.871714E-5	45.0	3
CGGTCTA	20	7.0102385E-4	45.0	31
GGTCTAA	20	7.0102385E-4	45.0	32
>>END_MODULE