##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936575.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	152818
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.095289821879625	33.0	31.0	34.0	30.0	34.0
2	32.22641966260519	33.0	31.0	34.0	30.0	34.0
3	32.14620659869911	33.0	31.0	34.0	30.0	34.0
4	35.85866193772985	37.0	35.0	37.0	35.0	37.0
5	35.91101833553639	37.0	35.0	37.0	35.0	37.0
6	35.89253229331623	37.0	35.0	37.0	35.0	37.0
7	26.855514402753602	36.0	10.0	37.0	0.0	37.0
8	31.200656990668637	36.0	19.0	37.0	17.0	37.0
9	36.010803701134684	39.0	32.0	39.0	32.0	39.0
10	36.76173618291039	38.0	35.0	39.0	33.0	39.0
11	37.11628211336361	39.0	37.0	39.0	34.0	39.0
12	37.132595636639664	39.0	37.0	39.0	34.0	39.0
13	37.06480911934458	39.0	37.0	39.0	33.0	39.0
14	38.25411927914251	40.0	37.0	41.0	34.0	41.0
15	38.3607624756246	40.0	37.0	41.0	34.0	41.0
16	38.30324961719169	40.0	37.0	41.0	34.0	41.0
17	38.19849101545629	40.0	37.0	41.0	34.0	41.0
18	38.112709235823004	40.0	37.0	41.0	33.0	41.0
19	38.01524035126752	40.0	37.0	41.0	34.0	41.0
20	38.04650630161368	40.0	37.0	41.0	34.0	41.0
21	37.99580546794226	40.0	36.0	41.0	34.0	41.0
22	38.000346817783246	40.0	36.0	41.0	34.0	41.0
23	37.954311664856235	40.0	36.0	41.0	34.0	41.0
24	37.90408852360324	40.0	36.0	41.0	33.0	41.0
25	37.820819536965544	40.0	36.0	41.0	33.0	41.0
26	37.663331544713316	40.0	36.0	41.0	33.0	41.0
27	37.5616026907825	40.0	36.0	41.0	33.0	41.0
28	37.524552081561076	40.0	36.0	41.0	33.0	41.0
29	37.49699642712246	40.0	36.0	41.0	33.0	41.0
30	37.4147024565169	40.0	36.0	41.0	33.0	41.0
31	37.296195474355116	40.0	35.0	41.0	32.0	41.0
32	37.166498710884845	39.0	35.0	41.0	32.0	41.0
33	37.05389417476999	39.0	35.0	41.0	31.0	41.0
34	36.93650617073905	39.0	35.0	41.0	31.0	41.0
35	36.85126097711002	39.0	35.0	41.0	31.0	41.0
36	36.711800965854806	39.0	35.0	41.0	31.0	41.0
37	36.68090146448717	39.0	35.0	41.0	31.0	41.0
38	36.565954272402465	39.0	35.0	41.0	30.0	41.0
39	36.52114934104621	39.0	35.0	41.0	30.0	41.0
40	36.33690402963002	39.0	35.0	41.0	30.0	41.0
41	36.25542802549438	39.0	35.0	40.0	30.0	41.0
42	36.19820309125888	39.0	35.0	40.0	30.0	41.0
43	36.16604064966169	39.0	35.0	40.0	30.0	41.0
44	35.96921174207227	38.0	35.0	40.0	29.0	41.0
45	35.851064665157246	38.0	35.0	40.0	29.0	41.0
46	35.735214438089756	38.0	35.0	40.0	28.0	41.0
47	35.647482626392176	38.0	35.0	40.0	28.0	41.0
48	35.56841471554398	38.0	35.0	40.0	28.0	41.0
49	35.41597848420997	38.0	34.0	40.0	27.0	41.0
50	35.194309570862075	38.0	34.0	40.0	27.0	41.0
51	33.12628093549189	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	15.0
11	12.0
12	4.0
13	5.0
14	10.0
15	7.0
16	22.0
17	36.0
18	70.0
19	96.0
20	165.0
21	250.0
22	404.0
23	560.0
24	808.0
25	1052.0
26	1322.0
27	1665.0
28	1935.0
29	2228.0
30	2735.0
31	3525.0
32	4681.0
33	6711.0
34	11070.0
35	13677.0
36	13875.0
37	22657.0
38	29804.0
39	33394.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64615424884503	20.41513434281302	24.87468753680849	12.064023871533458
2	32.100276145480244	24.989202842597077	25.36677616511144	17.54374484681124
3	33.727047860854086	23.691580834718422	25.892892198562993	16.68847910586449
4	29.325079506340877	26.672905024277245	25.47278461961287	18.529230849769007
5	26.36927587064351	31.05066157128087	23.36439424675104	19.21566831132458
6	27.816749335811224	35.73728225732571	22.88342996243898	13.562538444424085
7	61.695611773482185	28.344828488790586	5.744742111531364	4.214817626195868
8	82.76839115810965	6.429870826735071	7.181745605884124	3.619992409271159
9	75.97992383096232	9.43148058474787	9.106911489484224	5.481684094805585
10	43.58190789043175	26.697116831786833	16.266408407386564	13.45456687039485
11	34.52603750867044	26.6984255781387	22.174089439725687	16.601447473465168
12	33.08249028255833	23.003834626810978	25.658626601578348	18.255048489052335
13	25.99628316036069	26.37320210969912	26.491643654543314	21.138871075396878
14	21.06819877239592	28.725673677184627	28.414846418615607	21.791281131803846
15	21.409781570233875	26.271119894253296	32.84298970016621	19.476108835346622
16	24.406156342839193	24.946014212985382	31.85161433862503	18.79621510555039
17	24.208535643706895	24.867489431873206	27.682602834744596	23.2413720896753
18	25.039589577144056	26.646075724063916	27.943697731942574	20.370636966849453
19	27.72840895706003	27.123768142496303	25.21038097606303	19.937441924380636
20	29.228886649478465	25.603659254799826	26.04797864125954	19.11947545446217
21	26.893428784567263	26.73114423693544	26.369930243819447	20.005496734677852
22	24.723527333167556	26.219424413354446	26.356188407124815	22.70085984635318
23	25.541493803086023	26.382363334162207	27.34232878325852	20.733814079493254
24	24.502349199701605	24.673140598620584	28.326506039864412	22.498004161813398
25	23.731497598450442	25.63114292818909	26.952322370401394	23.685037102959075
26	22.285987252810532	29.011634755068123	26.81752149615883	21.884856495962516
27	22.844821945058825	28.465233153162583	27.10282820086639	21.587116700912194
28	21.789972385451975	28.76820793362039	28.81532280228769	20.626496878639948
29	23.648392205106727	27.117224410736956	27.854702980015446	21.379680404140874
30	23.546309989660905	26.94512426546611	27.70746901543012	21.801096729442868
31	24.82626392178932	27.248099045923908	25.70246960436598	22.223167427920796
32	26.587182138229792	27.11591566438509	26.051250507139212	20.245651690245914
33	24.792890889816643	28.556191024617515	25.5166276224005	21.134290463165335
34	23.248570194610583	28.030729364341894	27.005326597652108	21.71537384339541
35	23.054221361357953	27.696344671439228	27.069455168893718	22.1799787983091
36	23.385988561556882	30.332814197280427	25.27974453271212	21.001452708450575
37	24.090748472038634	29.754348309754086	25.341255611249984	20.813647606957293
38	22.97962281930139	29.867554869190805	25.00752529152325	22.145297019984554
39	24.35315211558848	26.99681974636496	26.515201088876967	22.1348270491696
40	24.26284861730948	26.75077543221348	27.475166537973276	21.511209412503764
41	20.652017432501406	28.382782132994805	27.56874190213195	23.39645853237184
42	22.60139512361109	27.605386799984295	26.219424413354446	23.573793663050164
43	23.394495412844037	26.316271643392795	26.52174482063631	23.767488123126856
44	22.52156159614705	28.067374262194246	26.158567707992514	23.25249643366619
45	22.084440314622622	27.821984321218707	25.979269457786387	24.114305906372284
46	21.930662618277953	29.600570613409417	25.820911149210175	22.64785561910246
47	22.634768155583767	27.907052834090223	27.16761114528393	22.290567865042078
48	22.575874569749637	26.288787970003536	28.251253124631916	22.884084335614915
49	22.06350037299271	26.78283971783429	27.814786216283423	23.33887369288958
50	19.99437239068696	27.746731405986207	27.75523825727336	24.503657946053476
51	19.927626326741617	27.2291222238218	27.302412019526496	25.54083942991009
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	88.0
1	218.0
2	348.0
3	471.0
4	594.0
5	448.5
6	303.0
7	276.5
8	250.0
9	267.5
10	285.0
11	287.5
12	290.0
13	299.5
14	309.0
15	326.0
16	343.0
17	368.5
18	394.0
19	386.0
20	378.0
21	406.5
22	435.0
23	539.5
24	644.0
25	877.0
26	1321.0
27	1532.0
28	1778.0
29	2024.0
30	2475.5
31	2927.0
32	3388.0
33	3849.0
34	4249.5
35	4650.0
36	4876.5
37	5103.0
38	5843.5
39	6584.0
40	7131.0
41	7678.0
42	8576.0
43	9474.0
44	9959.0
45	10444.0
46	11062.0
47	11680.0
48	11644.0
49	11608.0
50	11338.5
51	11069.0
52	10232.5
53	9396.0
54	8965.5
55	8535.0
56	8315.5
57	8096.0
58	8146.5
59	8197.0
60	7916.5
61	7636.0
62	6883.0
63	6130.0
64	5059.0
65	3988.0
66	3203.0
67	2418.0
68	2052.5
69	1687.0
70	1331.5
71	976.0
72	756.0
73	536.0
74	438.5
75	275.0
76	209.0
77	166.0
78	123.0
79	89.0
80	55.0
81	46.0
82	37.0
83	29.5
84	22.0
85	21.5
86	21.0
87	16.5
88	12.0
89	9.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	152818.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.869949875014726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.05253919069499	28.31669044222539
2	10.518523041228459	9.018571110733028
3	6.0125471280509215	7.732727820021202
4	4.298383526933586	7.370859453729272
5	2.976508479233129	6.380138465364029
6	2.223986079098805	5.720530304021778
7	1.7797994291209378	5.3409938619796105
8	1.4439882160792514	4.952296195474355
9	1.1203883198754447	4.322789200225104
>10	3.5458611267992617	19.77384863039694
>50	0.01831697525681926	0.5084479577013179
>100	0.00915848762840963	0.5621065581279693
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205	0.1341465010666283	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	161	0.10535408132549831	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.047769241843238365	0.0
2	0.0	0.0	0.0	0.2617492703739088	0.0
3	0.0	0.0	0.0	0.34878090277323354	0.0
4	0.0	0.0	0.0	0.45151749139499275	0.0
5	0.0	0.0	0.0	0.8336714261408996	0.0
6	0.0	0.0	0.0	1.0731720085330263	0.0
7	0.0	0.0	0.0	1.2982763810545879	0.0
8	0.0	0.0	0.0	1.75110261880145	0.0
9	0.0	0.0	0.0	1.9533039301652946	0.0
10	0.0	0.0	0.0	2.408747660615896	0.0
11	0.0	0.0	0.0	2.955149262521431	0.0
12	0.0	0.0	0.0	3.396851156277402	0.0
13	0.0	0.0	0.0	3.5761494064835295	0.0
14	0.0	0.0	0.0	3.6566373071235065	0.0
15	0.0	0.0	0.0	3.8248112133387426	0.0
16	0.0	0.0	0.0	4.178827101519454	0.0
17	0.0	0.0	0.0	4.572759753432187	0.0
18	6.54373175934772E-4	0.0	0.0	5.015770393540028	0.0
19	6.54373175934772E-4	0.0	0.0	5.281445902969545	0.0
20	6.54373175934772E-4	0.0	0.0	5.532725202528498	0.0
21	6.54373175934772E-4	0.0	0.0	5.883469224829536	0.0
22	6.54373175934772E-4	0.0	0.0	6.194950856574487	0.0
23	6.54373175934772E-4	0.0	0.0	6.547003625227394	0.0
24	6.54373175934772E-4	0.0	0.0	6.8022091638419555	0.0
25	6.54373175934772E-4	0.0	0.0	7.050216597521234	0.0
26	6.54373175934772E-4	0.0	0.0	7.272049104163123	0.0
27	6.54373175934772E-4	0.0	0.0	7.497153476684684	0.0
28	6.54373175934772E-4	0.0	0.0	7.738617178604614	0.0
29	6.54373175934772E-4	0.0	0.0	7.989896478163567	0.0
30	6.54373175934772E-4	0.0	0.0	8.285673153686083	0.0
31	6.54373175934772E-4	0.0	0.0	8.606316009894122	0.0
32	6.54373175934772E-4	0.0	0.0	8.876572131555184	0.0
33	6.54373175934772E-4	0.0	0.0	9.174311926605505	0.0
34	6.54373175934772E-4	0.0	0.0	9.460273004489	0.0
35	6.54373175934772E-4	0.0	0.0	9.763247784946799	0.0
36	6.54373175934772E-4	0.0	0.0	10.061641953173055	0.0
37	6.54373175934772E-4	0.0	0.0	10.364616733630855	0.0
38	6.54373175934772E-4	0.0	0.0	10.653849677394025	0.0
39	6.54373175934772E-4	0.0	0.0	10.975146906777997	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGGGA	70	0.0	45.000004	4
CGAATAT	35	1.2026976E-7	45.000004	14
GCTACGA	35	1.2026976E-7	45.000004	10
TTGGGAC	35	1.2026976E-7	45.000004	5
ATGGGCG	20	7.0140796E-4	45.0	5
GGATGTC	20	7.0140796E-4	45.0	8
GCAACGG	20	7.0140796E-4	45.0	2
TACGGGA	20	7.0140796E-4	45.0	4
TAACGGG	20	7.0140796E-4	45.0	3
ACGGGTG	20	7.0140796E-4	45.0	5
CGAATGG	20	7.0140796E-4	45.0	2
CATACGA	20	7.0140796E-4	45.0	18
CGTGTAT	20	7.0140796E-4	45.0	30
GCATGTT	20	7.0140796E-4	45.0	18
ACATACG	20	7.0140796E-4	45.0	17
GTAGTAG	20	7.0140796E-4	45.0	1
ACGAAAA	20	7.0140796E-4	45.0	21
AGGCGAT	30	2.1531214E-6	44.999996	7
ATAGGAT	45	1.909575E-8	40.0	5
CCTCGTC	45	1.909575E-8	40.0	36
>>END_MODULE