##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936574.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	217374
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.983208663409606	33.0	31.0	34.0	30.0	34.0
2	32.1092908995556	33.0	31.0	34.0	30.0	34.0
3	31.994474960206833	33.0	31.0	34.0	30.0	34.0
4	35.74456466734752	37.0	35.0	37.0	35.0	37.0
5	35.81056152069705	37.0	35.0	37.0	35.0	37.0
6	35.79882598654853	37.0	35.0	37.0	35.0	37.0
7	26.825222887741866	35.0	10.0	37.0	0.0	37.0
8	31.167917966270114	35.0	19.0	37.0	17.0	37.0
9	35.919999631970704	39.0	32.0	39.0	32.0	39.0
10	36.614112083321835	38.0	35.0	39.0	32.0	39.0
11	36.97205737576711	39.0	37.0	39.0	34.0	39.0
12	37.024745369731434	39.0	37.0	39.0	33.0	39.0
13	36.99389071370081	39.0	37.0	39.0	33.0	39.0
14	38.08200152732157	40.0	37.0	41.0	33.0	41.0
15	38.198491999963196	40.0	37.0	41.0	33.0	41.0
16	38.14901966196509	40.0	37.0	41.0	33.0	41.0
17	38.078201624849335	40.0	37.0	41.0	33.0	41.0
18	37.98096828507549	40.0	37.0	41.0	33.0	41.0
19	37.89060329202204	40.0	37.0	41.0	33.0	41.0
20	37.93116932107796	40.0	37.0	41.0	33.0	41.0
21	37.84170599979758	40.0	36.0	41.0	33.0	41.0
22	37.85407178411401	40.0	36.0	41.0	33.0	41.0
23	37.81518488871714	40.0	36.0	41.0	33.0	41.0
24	37.79220605960234	40.0	36.0	41.0	33.0	41.0
25	37.654678112377745	40.0	36.0	41.0	33.0	41.0
26	37.53469596179856	39.0	36.0	41.0	33.0	41.0
27	37.40527846016543	39.0	36.0	41.0	32.0	41.0
28	37.325236688840434	39.0	36.0	41.0	32.0	41.0
29	37.29952524220928	39.0	36.0	41.0	32.0	41.0
30	37.17765694149254	39.0	35.0	41.0	32.0	41.0
31	37.06563342442058	39.0	35.0	41.0	31.0	41.0
32	36.937573030813255	39.0	35.0	41.0	31.0	41.0
33	36.84119995951678	39.0	35.0	41.0	31.0	41.0
34	36.687736343812965	39.0	35.0	41.0	30.0	41.0
35	36.59641447459218	39.0	35.0	41.0	30.0	41.0
36	36.44632292730501	39.0	35.0	41.0	30.0	41.0
37	36.36140016745333	39.0	35.0	41.0	30.0	41.0
38	36.21035174399882	39.0	35.0	41.0	29.0	41.0
39	36.13256415210651	39.0	35.0	41.0	29.0	41.0
40	35.948710517357185	39.0	35.0	40.0	28.0	41.0
41	35.846522583197626	39.0	35.0	40.0	28.0	41.0
42	35.76189424678204	38.0	35.0	40.0	28.0	41.0
43	35.7223586997525	38.0	35.0	40.0	28.0	41.0
44	35.53568964089541	38.0	35.0	40.0	27.0	41.0
45	35.429881218545	38.0	34.0	40.0	27.0	41.0
46	35.319334419019754	38.0	34.0	40.0	26.0	41.0
47	35.22303035321612	38.0	34.0	40.0	26.0	41.0
48	35.11143927056594	38.0	34.0	40.0	26.0	41.0
49	34.922483829712846	38.0	34.0	40.0	24.0	41.0
50	34.68589619733731	37.0	34.0	40.0	24.0	41.0
51	32.556211874465205	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	14.0
10	17.0
11	16.0
12	14.0
13	9.0
14	5.0
15	21.0
16	25.0
17	63.0
18	86.0
19	197.0
20	306.0
21	523.0
22	732.0
23	1078.0
24	1421.0
25	1889.0
26	2367.0
27	2799.0
28	3213.0
29	3563.0
30	4263.0
31	5603.0
32	7096.0
33	9762.0
34	15312.0
35	18614.0
36	20624.0
37	32391.0
38	40736.0
39	44601.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.916356141948896	20.74857158629827	24.048874290393517	12.286197981359317
2	31.424641401455556	24.0576149861529	26.298453356887208	18.21929025550434
3	31.96886472163184	24.66670346959618	26.746989060329202	16.617442748442777
4	28.01853027500989	26.13882065012375	26.243708999236336	19.59894007563002
5	25.507650408972555	29.538951300523518	25.310294699458076	19.643103591045847
6	26.6756833843974	34.47928455105026	24.62898046684516	14.216051597707176
7	61.96785264107023	27.902601047043348	5.998417474030933	4.131128837855493
8	83.06191172817357	5.079724346057946	7.550581026249689	4.307782899518801
9	76.52387130015549	7.7930203244178236	9.91240902775861	5.770699347668074
10	41.46632071912924	26.43094390313469	17.399505000598047	14.703230377138022
11	31.04787141056428	26.28649240479542	24.266471611140247	18.39916457350005
12	27.819794455638668	23.623340417897264	29.030610836622593	19.52625428984147
13	25.15250213917028	25.263370964328765	30.19220329938263	19.39192359711833
14	20.799635650997818	27.75170903603927	30.771389402596444	20.677265910366465
15	20.47346968818718	25.85129776330196	33.76576775511331	19.909464793397554
16	24.182744946497742	25.65532216364423	29.688923238289767	20.473009651568262
17	24.797813905986917	24.64508174850718	27.477987247784924	23.079117097720978
18	25.382980485246627	24.495109810740935	29.698584007286982	20.42332569672546
19	25.290973161463654	26.650381370357078	27.115938428698925	20.942707039480343
20	26.115818819178006	26.522951226917662	26.947565026176086	20.41366492772825
21	25.183324592637575	26.712026277291674	28.735727363898167	19.368921766172587
22	24.276132380137458	24.730648559625344	27.84647657953573	23.146742480701462
23	22.881301351587584	26.505929872017813	28.16528195644373	22.447486819950868
24	23.290733942421816	25.47314766255394	29.287771306596007	21.94834708842824
25	22.677045092789385	26.54825324095798	27.809213613403628	22.965488052849008
26	21.11844102790582	27.786671819076798	27.20932586233864	23.88556129067874
27	21.65530376217947	26.14434108955073	29.879378398520522	22.32097674974928
28	21.34523908103085	26.43002382989686	28.98920754092026	23.235529548152034
29	21.59963933129077	26.07901588966482	28.541131874097182	23.780212904947234
30	22.123161003615888	26.00633010387627	29.357696872671063	22.51281201983678
31	23.346858409929432	25.779992087370157	27.95504522159964	22.918104281100778
32	23.91408356105146	26.004029920781697	27.120078758269155	22.961807759897688
33	23.29395419875422	25.59183711023397	28.290871953407493	22.823336737604315
34	21.014932788649975	25.387580851435775	30.096515682648338	23.50097067726591
35	21.354439813409147	25.682464324160204	29.695363750954577	23.26773211147607
36	24.21770772953527	25.678784031208885	28.28903180693183	21.81447643232401
37	21.822297054845567	26.637500345027465	28.48270722349499	23.05749537663198
38	22.08865825719727	26.842216640444576	28.36309770257712	22.70602739978102
39	22.739150036342892	25.42806407390028	28.22186646057026	23.610919429186563
40	23.644502102367348	23.858419130162762	29.157580943443094	23.339497824026793
41	21.052655791400994	24.57883647538344	28.525490629054072	25.843017104161493
42	22.086358074102698	24.731568632863176	29.282250867169026	23.8998224258651
43	22.05783580372998	24.892121412864466	29.24268771794235	23.807355065463213
44	21.238970622061515	25.218287375675104	28.90778105937233	24.634960942891055
45	21.0899187575331	25.124439905416473	28.293172136502065	25.492469200548364
46	20.976289712661128	25.835196481639937	28.789551648311207	24.39896215738773
47	20.4339065389605	25.161702871548574	30.450743879212784	23.953646710278136
48	20.786754625668202	25.252330085474807	29.920321657603942	24.04059363125305
49	22.268532575192985	24.431624757330685	30.029350336286768	23.270492331189562
50	20.0378150100748	25.667743152354927	30.025210006716534	24.269231830853734
51	20.270593539245723	25.184244665875404	28.5572331557592	25.987928639119673
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	216.0
2	371.0
3	667.0
4	963.0
5	715.5
6	468.0
7	419.5
8	371.0
9	397.0
10	423.0
11	420.5
12	418.0
13	412.0
14	406.0
15	408.0
16	410.0
17	460.5
18	511.0
19	565.0
20	619.0
21	752.0
22	885.0
23	1015.5
24	1146.0
25	1246.5
26	1704.5
27	2062.0
28	2484.0
29	2906.0
30	3359.0
31	3812.0
32	4279.0
33	4746.0
34	5464.5
35	6183.0
36	7006.5
37	7830.0
38	8481.0
39	9132.0
40	10216.0
41	11300.0
42	12375.5
43	13451.0
44	14522.0
45	15593.0
46	16727.0
47	17861.0
48	18339.0
49	18817.0
50	18521.0
51	18225.0
52	16287.0
53	14349.0
54	13401.0
55	12453.0
56	11683.0
57	10913.0
58	10275.5
59	9638.0
60	8820.0
61	8002.0
62	7293.5
63	6585.0
64	5830.0
65	5075.0
66	4414.0
67	3753.0
68	3125.0
69	2497.0
70	2076.5
71	1656.0
72	1301.0
73	946.0
74	757.0
75	431.0
76	294.0
77	222.5
78	151.0
79	118.5
80	86.0
81	61.5
82	37.0
83	29.0
84	21.0
85	17.0
86	13.0
87	9.0
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	3.0
94	5.0
95	3.0
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	217374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.94694306807736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.92565981986537	32.82795970560964
2	9.861694454268772	9.259528161986605
3	5.38617902198083	7.585939196982198
4	3.631681457838296	6.8198537057010675
5	2.5260505560806723	5.929517582170223
6	1.9132793861105304	5.389357104783424
7	1.5734073206221137	5.170666473289976
8	1.2475300702103445	4.685417854550367
9	0.9656362561006052	4.080030330566382
>10	2.9588854223873726	17.565624024776383
>50	0.004998117267545272	0.18396272021670546
>100	0.004998117267545272	0.5021431393670277
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	438	0.20149603908471114	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	218	0.1002879829234407	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6003661891486563E-4	0.0	0.0	0.08464673788033528	0.0
2	4.6003661891486563E-4	0.0	0.0	0.4443953738717602	0.0
3	4.6003661891486563E-4	0.0	0.0	0.6182892158215794	0.0
4	4.6003661891486563E-4	0.0	0.0	0.8266858041900135	0.0
5	4.6003661891486563E-4	0.0	0.0	1.4665967411005916	0.0
6	4.6003661891486563E-4	0.0	0.0	1.9565357402449235	0.0
7	4.6003661891486563E-4	0.0	0.0	2.3503270860360486	0.0
8	4.6003661891486563E-4	0.0	0.0	3.163671828277531	0.0
9	4.6003661891486563E-4	0.0	0.0	3.484317351661192	0.0
10	4.6003661891486563E-4	0.0	0.0	4.17897264622264	0.0
11	4.6003661891486563E-4	0.0	0.0	5.01393910955312	0.0
12	4.6003661891486563E-4	0.0	0.0	5.638668838039508	0.0
13	4.6003661891486563E-4	0.0	0.0	5.911010516437108	0.0
14	4.6003661891486563E-4	0.0	0.0	6.055922051395291	0.0
15	4.6003661891486563E-4	0.0	0.0	6.28272010452032	0.0
16	4.6003661891486563E-4	0.0	0.0	6.757937931859376	0.0
17	4.6003661891486563E-4	0.0	0.0	7.355065463210871	0.0
18	4.6003661891486563E-4	0.0	0.0	8.003257059261918	0.0
19	4.6003661891486563E-4	0.0	0.0	8.396128331815213	0.0
20	4.6003661891486563E-4	0.0	0.0	8.754496857949892	0.0
21	4.6003661891486563E-4	0.0	0.0	9.223734209243055	0.0
22	4.6003661891486563E-4	0.0	0.0	9.704932512628005	0.0
23	4.6003661891486563E-4	0.0	0.0	10.189811108964273	0.0
24	4.6003661891486563E-4	0.0	0.0	10.567501173093378	0.0
25	4.6003661891486563E-4	0.0	0.0	10.941050907652249	0.0
26	4.6003661891486563E-4	0.0	0.0	11.264916687368315	0.0
27	4.6003661891486563E-4	0.0	0.0	11.617764774076017	0.0
28	4.6003661891486563E-4	0.0	0.0	11.943470700267742	0.0
29	4.6003661891486563E-4	0.0	0.0	12.352443254483056	0.0
30	4.6003661891486563E-4	0.0	0.0	12.823060715632964	0.0
31	4.6003661891486563E-4	0.0	0.0	13.206271219189047	0.0
32	9.200732378297313E-4	0.0	0.0	13.581661100223577	0.0
33	9.200732378297313E-4	0.0	0.0	13.977752629109277	0.0
34	9.200732378297313E-4	0.0	0.0	14.376144341089551	0.0
35	9.200732378297313E-4	0.0	0.0	14.778676382640057	0.0
36	9.200732378297313E-4	0.0	0.0	15.130144359491016	0.0
37	9.200732378297313E-4	0.0	0.0	15.518875302474077	0.0
38	9.200732378297313E-4	0.0	0.0	15.93796866230552	0.0
39	9.200732378297313E-4	0.0	0.0	16.48449216557638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACACG	30	2.157356E-6	45.000004	40
CACAACG	30	2.157356E-6	45.000004	12
GATCGAA	20	7.0206536E-4	45.0	44
CCCACCG	20	7.0206536E-4	45.0	15
AGACGGC	20	7.0206536E-4	45.0	35
TATAGCG	20	7.0206536E-4	45.0	1
CGTTTTA	35	1.2058626E-7	45.0	1
AATACGG	25	3.880338E-5	45.0	2
AGAGACG	20	7.0206536E-4	45.0	33
CGAGATA	80	0.0	42.1875	19
AGGCGAT	70	0.0	41.785713	7
ACAAGCG	85	0.0	39.705883	14
AACGAGC	40	3.4410004E-7	39.375	15
TGACGGG	70	0.0	38.571426	3
AGTTACG	35	6.22494E-6	38.571426	1
CGTCATA	35	6.22494E-6	38.571426	38
AACACGT	35	6.22494E-6	38.571426	41
TACGGGA	65	9.094947E-12	38.076927	4
GTTGATC	30	1.1368787E-4	37.500004	16
CACGGGC	30	1.1368787E-4	37.500004	4
>>END_MODULE