##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936573.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128315
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05901102754939	33.0	31.0	34.0	30.0	34.0
2	32.19501227448077	33.0	31.0	34.0	30.0	34.0
3	32.08559404590266	33.0	31.0	34.0	30.0	34.0
4	35.799415500915714	37.0	35.0	37.0	35.0	37.0
5	35.89109613061606	37.0	35.0	37.0	35.0	37.0
6	35.87064645598722	37.0	35.0	37.0	35.0	37.0
7	26.837657327670186	36.0	10.0	37.0	0.0	37.0
8	31.245723415033318	37.0	19.0	37.0	17.0	37.0
9	36.13887698242606	39.0	32.0	39.0	32.0	39.0
10	36.7274909402642	38.0	35.0	39.0	33.0	39.0
11	37.088952967307016	39.0	37.0	39.0	34.0	39.0
12	37.21525152943927	39.0	37.0	39.0	34.0	39.0
13	37.23312161477614	39.0	37.0	39.0	34.0	39.0
14	38.450274714569616	40.0	38.0	41.0	34.0	41.0
15	38.53042122900674	40.0	38.0	41.0	34.0	41.0
16	38.45571445271403	40.0	38.0	41.0	34.0	41.0
17	38.39376534310096	40.0	38.0	41.0	34.0	41.0
18	38.310470326929824	40.0	37.0	41.0	34.0	41.0
19	38.196789151697	40.0	37.0	41.0	34.0	41.0
20	38.29175856291158	40.0	37.0	41.0	34.0	41.0
21	38.2252815337256	40.0	37.0	41.0	34.0	41.0
22	38.24255932665705	40.0	37.0	41.0	34.0	41.0
23	38.169574874332696	40.0	37.0	41.0	34.0	41.0
24	38.150192884697816	40.0	37.0	41.0	34.0	41.0
25	38.064146826169974	40.0	37.0	41.0	34.0	41.0
26	37.956879554222034	40.0	37.0	41.0	34.0	41.0
27	37.8830845965008	40.0	37.0	41.0	33.0	41.0
28	37.904181116782915	40.0	37.0	41.0	33.0	41.0
29	37.915652885477144	40.0	37.0	41.0	33.0	41.0
30	37.85641585161517	40.0	37.0	41.0	33.0	41.0
31	37.72823909909208	40.0	36.0	41.0	33.0	41.0
32	37.60884541947551	40.0	36.0	41.0	33.0	41.0
33	37.54737949577213	40.0	36.0	41.0	33.0	41.0
34	37.46409227292211	40.0	36.0	41.0	33.0	41.0
35	37.397358064139034	40.0	36.0	41.0	32.0	41.0
36	37.27348322487628	40.0	36.0	41.0	32.0	41.0
37	37.22991076647313	40.0	36.0	41.0	32.0	41.0
38	37.12969645014223	40.0	36.0	41.0	31.0	41.0
39	37.11615165802907	40.0	36.0	41.0	31.0	41.0
40	36.987967112184855	39.0	35.0	41.0	31.0	41.0
41	36.95970852978997	39.0	35.0	41.0	31.0	41.0
42	36.87464442972372	39.0	35.0	41.0	31.0	41.0
43	36.83475041889101	39.0	35.0	41.0	31.0	41.0
44	36.65110859992986	39.0	35.0	41.0	30.0	41.0
45	36.55409733858084	39.0	35.0	41.0	30.0	41.0
46	36.481346685890195	39.0	35.0	40.0	30.0	41.0
47	36.39102988738651	39.0	35.0	40.0	30.0	41.0
48	36.324911350972215	39.0	35.0	40.0	30.0	41.0
49	36.197778903479715	39.0	35.0	40.0	29.0	41.0
50	35.997256750964425	39.0	35.0	40.0	29.0	41.0
51	34.033285274519734	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	6.0
10	6.0
11	1.0
12	3.0
13	1.0
14	3.0
15	6.0
16	7.0
17	14.0
18	23.0
19	52.0
20	95.0
21	146.0
22	217.0
23	363.0
24	523.0
25	743.0
26	966.0
27	1149.0
28	1325.0
29	1617.0
30	2011.0
31	2678.0
32	3543.0
33	4802.0
34	7936.0
35	10338.0
36	11605.0
37	19401.0
38	26687.0
39	32029.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.82956006702256	22.32085103066672	28.604605852784164	11.244983049526555
2	30.282507890737637	24.877839691384484	28.09414331917547	16.745509098702414
3	31.198223122783773	24.220083388536022	28.2578030627752	16.323890425905
4	27.813583758718778	26.671082881970154	27.60706074893816	17.90827261037291
5	25.335307641351363	31.33304757822546	25.81070022990297	17.520944550520205
6	26.575225032147447	35.16424424268402	24.722752601020925	13.537778124147607
7	64.78354050578655	27.563418150644896	4.657288703581031	2.995752639987531
8	89.66917351829483	3.390094688851654	4.576238163893543	2.3644936289599814
9	83.89821922612322	6.964891088337295	6.060865837976854	3.076023847562639
10	36.10801543077582	39.15910065074231	12.81611658808401	11.91676733039785
11	27.19011806881503	28.647469118965045	28.419904142150177	15.74250867006975
12	25.775630284845885	25.71328371585551	30.103261504890312	18.407824494408292
13	22.382418267544715	28.101936640299265	31.797529517203753	17.718115574952265
14	20.043642598293264	30.287183883411917	29.95129174297627	19.717881775318553
15	18.746833963293458	28.809570198340023	34.12305654054475	18.320539297821767
16	22.396446245567546	27.723181233682737	32.07497174921093	17.80540077153879
17	22.980165997739938	26.591591006507425	29.323929392510617	21.104313603242023
18	23.888087908662275	26.770058060242373	30.49604488952967	18.845809141565677
19	23.132135759653977	28.870358103105637	28.780734910181977	19.21677122705841
20	24.899660990531114	28.205587811245763	29.027783189806332	17.866968008416787
21	23.054981880528388	28.576549896738495	29.947395082414367	18.421073140318747
22	23.005883957448468	25.28620971827144	30.02922495421424	21.678681370065853
23	20.48630323812493	28.217277792931455	30.47344425827066	20.822974710672952
24	20.47461325643923	27.659276000467596	31.8918287028017	19.97428204029147
25	19.89868682539064	29.540583719752174	30.193664029926353	20.367065424930832
26	20.257958929197677	31.60347582122121	28.853212796633287	19.285352452947823
27	20.280559560456688	29.662159529283404	30.60826871371235	19.449012196547557
28	18.419514476093987	29.436153216693295	32.20434087986595	19.939991427346765
29	19.959474730156256	27.374819779449012	31.402408136227255	21.26329735416748
30	21.0630090012859	28.495499357051006	30.693995246074113	19.747496395588982
31	22.754939017262206	28.68487706035927	28.43860811284729	20.12157580953123
32	21.84000311732845	29.44472586992947	28.53602462689475	20.17924638584733
33	22.131473327358453	29.08077777344816	28.748782293574404	20.038966605618985
34	20.665549623972257	29.263141487745003	29.314577407162062	20.75673148112068
35	19.710088454194754	29.777500681915598	29.734637415734717	20.77777344815493
36	22.278767096598216	29.591240307056854	28.877372092117053	19.252620504227878
37	21.552429567860344	29.32159139617348	28.979464598838796	20.146514437127383
38	20.625024354128513	29.888945173985892	28.69189104937069	20.794139422514903
39	21.03573237735261	28.50952733507384	29.609164945641588	20.845575341931966
40	21.634259439660212	28.627206484043178	29.66839418618244	20.070139890114174
41	20.081829871799865	29.701905467014768	29.146241670888053	21.070022990297314
42	22.26084245801348	28.317811635428434	28.450298094532982	20.971047812025095
43	22.456454818220784	27.43560768421463	28.63032381249269	21.477613685071894
44	21.299925963449322	28.329501617114133	28.735533647663953	21.635038771772592
45	20.823754042785332	28.908545376612242	28.254685734325687	22.01301484627674
46	22.076920079491877	29.530452402291235	28.195456493784825	20.197171024432063
47	20.666328956084634	29.44706386626661	29.010637883333985	20.87596929431477
48	21.83532712465417	28.167400537739155	28.842302147059968	21.154970190546702
49	21.698944004987723	27.33663250594241	30.28172855862526	20.682694930444608
50	20.345244125784205	28.17831118731247	29.47278182597514	22.003662860928188
51	20.243151619062463	27.966332852745197	28.450298094532982	23.340217433659355
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	127.0
2	210.0
3	337.0
4	464.0
5	349.0
6	234.0
7	220.5
8	207.0
9	209.5
10	212.0
11	208.5
12	205.0
13	200.5
14	196.0
15	216.5
16	237.0
17	271.5
18	306.0
19	351.0
20	396.0
21	452.5
22	509.0
23	760.0
24	1011.0
25	1220.5
26	1675.5
27	1921.0
28	2392.5
29	2864.0
30	3250.5
31	3637.0
32	3971.0
33	4305.0
34	5015.0
35	5725.0
36	5975.0
37	6225.0
38	6877.5
39	7530.0
40	8106.5
41	8683.0
42	9138.0
43	9593.0
44	9980.5
45	10368.0
46	10765.5
47	11163.0
48	11091.5
49	11020.0
50	10781.0
51	10542.0
52	9358.0
53	8174.0
54	7037.0
55	5900.0
56	5212.0
57	4524.0
58	4018.5
59	3513.0
60	3088.0
61	2663.0
62	2254.5
63	1846.0
64	1440.0
65	1034.0
66	809.5
67	585.0
68	459.0
69	333.0
70	270.5
71	208.0
72	158.0
73	108.0
74	94.0
75	67.0
76	54.0
77	39.5
78	25.0
79	21.5
80	18.0
81	13.0
82	8.0
83	5.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	128315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.760004675992676
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.67163473611132	36.75875774461287
2	11.703274789878728	12.349296652768578
3	6.165526817235114	9.758796711218485
4	3.949836777500406	8.33573627401317
5	2.657350920988493	7.010092350855317
6	1.7695977784014536	5.601839223785216
7	1.2703289561145659	4.691579316525737
8	0.9616094772448633	4.058761641273429
9	0.6248245912051877	2.966917351829482
>10	1.2171523951609329	8.055176713556483
>50	0.0073856334657823606	0.2564002649729182
>100	0.001477126693156472	0.1566457545883178
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	201	0.1566457545883178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07481588278845029	0.0
2	0.0	0.0	0.0	0.3429061294470639	0.0
3	0.0	0.0	0.0	0.4644819389782956	0.0
4	0.0	0.0	0.0	0.6273623504656509	0.0
5	0.0	0.0	0.0	1.098078946342984	0.0
6	0.0	0.0	0.0	1.3482445544168646	0.0
7	0.0	0.0	0.0	1.6077621478393018	0.0
8	0.0	0.0	0.0	2.2000545532478664	0.0
9	0.0	0.0	0.0	2.4065775630284847	0.0
10	0.0	0.0	0.0	3.0425125667303123	0.0
11	0.0	0.0	0.0	3.934068503292678	0.0
12	0.0	0.0	0.0	4.620660094299185	0.0
13	0.0	0.0	0.0	4.900440322643495	0.0
14	0.0	0.0	0.0	4.996298172466196	0.0
15	0.0	0.0	0.0	5.202821182246814	0.0
16	0.0	0.0	0.0	5.646261154190858	0.0
17	0.0	0.0	0.0	6.210497603553755	0.0
18	0.0	0.0	0.0	6.823831975996571	0.0
19	0.0	0.0	0.0	7.204925378950239	0.0
20	0.0	0.0	0.0	7.582901453454389	0.0
21	0.0	0.0	0.0	8.055176713556483	0.0
22	0.0	0.0	0.0	8.53758329111951	0.0
23	0.0	0.0	0.0	9.053501149514865	0.0
24	0.0	0.0	0.0	9.447063866266609	0.0
25	0.0	0.0	0.0	9.814129291197444	0.0
26	0.0	0.0	0.0	10.15080076374547	0.0
27	0.0	0.0	0.0	10.50461754276585	0.0
28	0.0	0.0	0.0	10.833495694190079	0.0
29	0.0	0.0	0.0	11.196664458559015	0.0
30	0.0	0.0	0.0	11.647897751626855	0.0
31	0.0	0.0	0.0	12.023535829793866	0.0
32	0.0	0.0	0.0	12.389821922612322	0.0
33	0.0	0.0	0.0	12.759225343880294	0.0
34	0.0	0.0	0.0	13.153567392744417	0.0
35	0.0	0.0	0.0	13.565834080193275	0.0
36	0.0	0.0	0.0	13.964072789619296	0.0
37	0.0	0.0	0.0	14.361532166932939	0.0
38	0.0	0.0	0.0	14.754315551572303	0.0
39	0.0	0.0	0.0	15.18996220239255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCA	20	7.0098514E-4	45.000004	22
CCGGGAT	20	7.0098514E-4	45.000004	5
ACACGGG	20	7.0098514E-4	45.000004	3
GCGCGAG	20	7.0098514E-4	45.000004	1
AATGGTC	20	7.0098514E-4	45.000004	10
CGCGAGG	20	7.0098514E-4	45.000004	2
CCTCGTG	20	7.0098514E-4	45.000004	34
ATCACAC	20	7.0098514E-4	45.000004	10
CGCTAGG	20	7.0098514E-4	45.000004	2
CGAGGGC	20	7.0098514E-4	45.000004	4
TAACGGG	25	3.8713944E-5	45.0	3
GCGATGC	25	3.8713944E-5	45.0	9
GCGATAC	25	3.8713944E-5	45.0	9
TGAGCGG	25	3.8713944E-5	45.0	2
ATGGGCG	30	2.150402E-6	44.999996	5
CAGGGCG	30	2.150402E-6	44.999996	5
AATGCGG	30	2.150402E-6	44.999996	2
CTAGCGG	30	2.150402E-6	44.999996	2
AGGGCGA	75	0.0	42.0	6
CGGGATA	70	0.0	41.785717	6
>>END_MODULE