FastQCFastQC Report
Sat 14 Jan 2017
SRR2936572.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936572.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192149
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.16861914451805632No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC2450.12750521730532036No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG2430.12646435838854223No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC2100.10929018626170314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTT207.0186064E-445.0000049
TTTCGTG207.0186064E-445.00000415
CGGGCTC302.1560372E-645.0000046
GAAACGT207.0186064E-445.0000049
GGCGATA501.0713848E-940.58
CAGTCTC451.9139407E-840.06
CGGGACC403.4382174E-739.3750046
AAGGGCG403.4382174E-739.3750045
AGGCGAT1100.038.8636367
CACGCCG659.094947E-1238.0769226
CGGTAGT659.094947E-1238.0769212
TACGGGA301.1363858E-437.5000044
TATGCGG301.1363858E-437.5000042
TGGGCGA1150.037.1739166
GCGATGC800.036.5625049
GGCGATG2400.036.5625048
AACGTTC250.002102752436.011
ATGGGCG751.8189894E-1236.05
CCCTACC250.002102752436.020
CCACTAG250.002102752436.033
Produced by FastQC (version 0.11.5)