##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936572.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192149
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07518644385347	33.0	31.0	34.0	30.0	34.0
2	32.198793644515455	33.0	31.0	34.0	30.0	34.0
3	32.08751021342812	33.0	31.0	34.0	30.0	34.0
4	35.82241906020848	37.0	35.0	37.0	35.0	37.0
5	35.86999672129441	37.0	35.0	37.0	35.0	37.0
6	35.84436557046875	37.0	35.0	37.0	35.0	37.0
7	26.735512544952094	36.0	10.0	37.0	0.0	37.0
8	31.124351414787483	36.0	19.0	37.0	17.0	37.0
9	35.92321063341469	39.0	32.0	39.0	32.0	39.0
10	36.656287568501526	38.0	35.0	39.0	33.0	39.0
11	37.02794185762091	39.0	37.0	39.0	34.0	39.0
12	37.05513950111632	39.0	37.0	39.0	34.0	39.0
13	37.049836324935335	39.0	37.0	39.0	33.0	39.0
14	38.1710911844454	40.0	37.0	41.0	33.0	41.0
15	38.25307443702543	40.0	37.0	41.0	33.0	41.0
16	38.23115915253267	40.0	37.0	41.0	33.0	41.0
17	38.16550697635689	40.0	37.0	41.0	33.0	41.0
18	38.034030882284064	40.0	37.0	41.0	33.0	41.0
19	37.92377269722975	40.0	37.0	41.0	33.0	41.0
20	37.991537817006595	40.0	37.0	41.0	33.0	41.0
21	37.94346574793519	40.0	37.0	41.0	33.0	41.0
22	37.972989711109605	40.0	37.0	41.0	33.0	41.0
23	37.907051298731716	40.0	36.0	41.0	33.0	41.0
24	37.87656974535387	40.0	36.0	41.0	33.0	41.0
25	37.751708309697165	40.0	36.0	41.0	33.0	41.0
26	37.626003778317866	40.0	36.0	41.0	33.0	41.0
27	37.49920114078137	40.0	36.0	41.0	33.0	41.0
28	37.445524046443126	40.0	36.0	41.0	33.0	41.0
29	37.38721512992522	40.0	36.0	41.0	33.0	41.0
30	37.272777896320044	40.0	36.0	41.0	32.0	41.0
31	37.16284758182452	39.0	35.0	41.0	32.0	41.0
32	37.01839197705947	39.0	35.0	41.0	31.0	41.0
33	36.92768632675684	39.0	35.0	41.0	31.0	41.0
34	36.78759712514767	39.0	35.0	41.0	31.0	41.0
35	36.651640133438114	39.0	35.0	41.0	30.0	41.0
36	36.53240974452118	39.0	35.0	41.0	30.0	41.0
37	36.41453767649064	39.0	35.0	41.0	30.0	41.0
38	36.28740716839536	39.0	35.0	41.0	30.0	41.0
39	36.24368068530151	39.0	35.0	41.0	30.0	41.0
40	36.0501069482537	39.0	35.0	41.0	29.0	41.0
41	35.93976549448605	39.0	35.0	40.0	28.0	41.0
42	35.81500294042644	39.0	35.0	40.0	28.0	41.0
43	35.76752936523219	39.0	35.0	40.0	28.0	41.0
44	35.573523671733916	38.0	35.0	40.0	27.0	41.0
45	35.480653034884384	38.0	34.0	40.0	26.0	41.0
46	35.375146370785174	38.0	34.0	40.0	26.0	41.0
47	35.22799494142566	38.0	34.0	40.0	26.0	41.0
48	35.13115342780863	38.0	34.0	40.0	26.0	41.0
49	34.97954191799072	38.0	34.0	40.0	24.0	41.0
50	34.778557265455454	38.0	34.0	40.0	24.0	41.0
51	32.747794680170074	36.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	12.0
10	20.0
11	12.0
12	10.0
13	5.0
14	7.0
15	21.0
16	29.0
17	39.0
18	90.0
19	183.0
20	281.0
21	453.0
22	615.0
23	899.0
24	1343.0
25	1654.0
26	1986.0
27	2353.0
28	2707.0
29	3081.0
30	3823.0
31	4675.0
32	6071.0
33	8460.0
34	13184.0
35	15977.0
36	17786.0
37	28616.0
38	36512.0
39	41227.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.71113562912115	19.38391560715903	24.019380793030408	11.885567970689413
2	32.563791640862036	24.410743745738984	25.126854680482335	17.898609932916642
3	33.32413907956846	24.961878542173004	25.520299351024466	16.193683027234073
4	29.058699238611702	25.93976549448605	25.9231117518176	19.078423515084648
5	26.094853473085987	29.65927483359268	24.64025313688856	19.60561855643277
6	27.42350988035327	35.09672181484161	24.286881534642387	13.192886770162737
7	61.523088852921425	28.409723704000537	6.240469635543251	3.8267178075347776
8	83.17763818703195	5.627403733560935	7.561319600934692	3.6336384784724354
9	76.59628725624385	8.09528022524187	9.531665530395683	5.776766988118595
10	41.49123857006802	26.33945531852885	17.640476921555667	14.528829189847462
11	32.80318919172101	26.840628886957518	23.27985053265955	17.076331388661924
12	28.776106042706438	25.049310691182363	27.30589282275734	18.868690443353856
13	25.93768377665249	26.178122186428244	29.45630734482095	18.42788669209832
14	22.756818926978543	28.213521798187863	29.28768820030289	19.741971074530703
15	21.776329827373548	26.3228015758604	32.95775674086256	18.943111855903492
16	26.287412372689943	26.211429671765142	29.132600221702948	18.368557733841968
17	26.78234078761794	25.4614908222265	26.590822746930765	21.16534564322479
18	26.93951048405144	25.52758536344191	28.588751437686376	18.94415271482027
19	26.627252809018003	26.559596979427425	26.791708517868944	20.02144169368563
20	27.669152584712904	27.42871417493716	26.386814399242255	18.515318841107682
21	26.380048816283196	27.236155275333207	27.744094426720928	18.63970148166267
22	25.196592227906468	25.38238554455136	27.72119553055181	21.699826696990357
23	23.727940296332534	27.12634465961311	28.6543255494434	20.491389494610953
24	23.9673378471915	25.587434751156657	28.88799837626009	21.557229025391752
25	23.564525446398367	27.568709699243815	27.418826015227765	21.44793883913005
26	22.80782101390067	27.73889013213704	27.541647367407585	21.911641486554707
27	22.488797755908173	27.22054239158153	28.916621996471488	21.374037856038804
28	20.950408276910107	28.157315416681843	28.986359543895624	21.905916762512426
29	23.526534095935965	27.607741908622994	27.858588907566524	21.007135087874516
30	23.88042612764053	27.393845401225093	28.05114780717048	20.674580663963905
31	25.128415968857503	26.968134104262838	26.393059552742926	21.510390374136737
32	24.703225101353638	28.861456473882246	26.338414459612068	20.096903965152045
33	24.36754810069269	27.350649756178797	27.121140365029223	21.16066177809929
34	24.050086131075364	26.990512570973568	28.55492352289109	20.404477775059977
35	22.7302770246007	29.490135259616235	27.772197617473935	20.007390098309124
36	24.523156508751022	28.96606279501845	26.250982310602712	20.25979838562782
37	23.172642064231404	30.451368469260835	25.93976549448605	20.436223972021715
38	22.704255551681246	29.26062586846666	26.5226464878818	21.512472091970295
39	23.721695142831862	27.582761294620322	26.887467538212533	21.808076024335282
40	24.658988597390564	26.2894940905235	27.7409718499706	21.31054546211534
41	22.33683235405857	27.351170185637187	27.049841529229923	23.26215593107432
42	22.822393038735562	27.75346215697193	26.952000791052775	22.472144013239724
43	22.888487579950976	26.76620747440788	27.337639019719074	23.007665925922073
44	22.598608371628266	27.239798281541926	27.1508048441574	23.010788502672405
45	22.18642824058413	27.17838760545202	27.35949705697141	23.27568709699244
46	22.828117762777843	27.46098080135728	26.709480663443475	23.0014207724214
47	21.761237373080267	28.151070263181175	28.33842486820124	21.749267495537318
48	22.537197695538357	27.112813493694997	27.89866197586248	22.45132683490416
49	22.567382604124926	25.998574023284014	29.534371763579305	21.899671609011754
50	21.472919453132725	27.539565649574026	28.449276342838107	22.538238554455138
51	21.916845781138598	26.632457103601894	27.309535828966062	24.14116128629345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	190.0
2	334.0
3	538.5
4	743.0
5	553.5
6	364.0
7	353.0
8	342.0
9	345.5
10	349.0
11	358.0
12	367.0
13	382.5
14	398.0
15	430.0
16	462.0
17	463.5
18	465.0
19	497.5
20	530.0
21	621.0
22	712.0
23	833.0
24	954.0
25	1202.0
26	1778.0
27	2106.0
28	2414.5
29	2723.0
30	3237.0
31	3751.0
32	4237.5
33	4724.0
34	5265.5
35	5807.0
36	6505.0
37	7203.0
38	7840.0
39	8477.0
40	9093.5
41	9710.0
42	10880.5
43	12051.0
44	12652.5
45	13254.0
46	14715.0
47	16176.0
48	15709.5
49	15243.0
50	14883.0
51	14523.0
52	13954.5
53	13386.0
54	12658.0
55	11930.0
56	10839.5
57	9749.0
58	9221.0
59	8693.0
60	8189.5
61	7686.0
62	6850.5
63	6015.0
64	5095.0
65	4175.0
66	3457.5
67	2740.0
68	2271.0
69	1802.0
70	1451.5
71	1101.0
72	902.5
73	704.0
74	548.0
75	305.0
76	218.0
77	176.5
78	135.0
79	103.0
80	71.0
81	53.5
82	36.0
83	27.0
84	18.0
85	15.5
86	13.0
87	10.0
88	7.0
89	6.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	192149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.10927457337795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90676093680953	31.519289717875193
2	11.049491825011048	10.41067088561481
3	6.483650022094564	9.163201473856226
4	4.295183384887317	8.093718936866702
5	3.0821917808219177	7.2599909445274236
6	2.2470172337604946	6.351321110180121
7	1.6217410517012816	5.3479331144060085
8	1.299160406539991	4.896200344524301
9	0.9600088378258949	4.070278794060859
>10	2.039328325231993	11.977163555365888
>50	0.008837825894829872	0.2622964470280876
>100	0.006628369421122405	0.647934675694383
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	324	0.16861914451805632	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	245	0.12750521730532036	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	243	0.12646435838854223	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	210	0.10929018626170314	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0010408589167781254	0.0	0.0	0.04371607450468126	0.0
2	0.0010408589167781254	0.0	0.0	0.2675007416119782	0.0
3	0.0010408589167781254	0.0	0.0	0.35597374953811883	0.0
4	0.0010408589167781254	0.0	0.0	0.515225163805172	0.0
5	0.0010408589167781254	0.0	0.0	1.047624499737183	0.0
6	0.0010408589167781254	0.0	0.0	1.3432284321021708	0.0
7	0.0010408589167781254	0.0	0.0	1.561288375167188	0.0
8	0.0010408589167781254	0.0	0.0	1.9989695496723896	0.0
9	0.0010408589167781254	0.0	0.0	2.1608231112313883	0.0
10	0.0010408589167781254	0.0	0.0	2.7202847789996305	0.0
11	0.0010408589167781254	0.0	0.0	3.2006411690927354	0.0
12	0.0010408589167781254	0.0	0.0	3.7340813639415247	0.0
13	0.0010408589167781254	0.0	0.0	3.9589068899655997	0.0
14	0.0010408589167781254	0.0	0.0	4.059349775434689	0.0
15	0.0010408589167781254	0.0	0.0	4.225887202119189	0.0
16	0.0010408589167781254	0.0	0.0	4.53085886473518	0.0
17	0.0010408589167781254	0.0	0.0	4.882148749147797	0.0
18	0.0010408589167781254	0.0	0.0	5.266746118897314	0.0
19	0.0010408589167781254	0.0	0.0	5.497816798422058	0.0
20	0.0010408589167781254	0.0	0.0	5.738255208197804	0.0
21	0.0010408589167781254	0.0	0.0	6.014603250602397	0.0
22	0.0010408589167781254	0.0	0.0	6.291471722465379	0.0
23	0.0010408589167781254	0.0	0.0	6.602688538582038	0.0
24	0.0010408589167781254	0.0	0.0	6.85717854373429	0.0
25	0.0010408589167781254	0.0	0.0	7.087208364342255	0.0
26	0.0010408589167781254	0.0	0.0	7.319319902783777	0.0
27	0.0010408589167781254	0.0	0.0	7.554554017975634	0.0
28	0.0010408589167781254	0.0	0.0	7.775216108332596	0.0
29	0.0010408589167781254	0.0	0.0	8.025022248359345	0.0
30	0.0010408589167781254	0.0	0.0	8.347688512560564	0.0
31	0.001561288375167188	0.0	0.0	8.600617229337649	0.0
32	0.001561288375167188	0.0	0.0	8.858229811240236	0.0
33	0.001561288375167188	0.0	0.0	9.124689693935435	0.0
34	0.001561288375167188	0.0	0.0	9.407282889840696	0.0
35	0.001561288375167188	0.0	0.0	9.706009398956018	0.0
36	0.001561288375167188	0.0	0.0	9.961540263025048	0.0
37	0.001561288375167188	0.0	0.0	10.212907691426965	0.0
38	0.001561288375167188	0.0	0.0	10.512154630000676	0.0
39	0.001561288375167188	0.0	0.0	10.939427215338098	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTT	20	7.0186064E-4	45.000004	9
TTTCGTG	20	7.0186064E-4	45.000004	15
CGGGCTC	30	2.1560372E-6	45.000004	6
GAAACGT	20	7.0186064E-4	45.000004	9
GGCGATA	50	1.0713848E-9	40.5	8
CAGTCTC	45	1.9139407E-8	40.0	6
CGGGACC	40	3.4382174E-7	39.375004	6
AAGGGCG	40	3.4382174E-7	39.375004	5
AGGCGAT	110	0.0	38.863636	7
CACGCCG	65	9.094947E-12	38.07692	26
CGGTAGT	65	9.094947E-12	38.07692	12
TACGGGA	30	1.1363858E-4	37.500004	4
TATGCGG	30	1.1363858E-4	37.500004	2
TGGGCGA	115	0.0	37.173916	6
GCGATGC	80	0.0	36.562504	9
GGCGATG	240	0.0	36.562504	8
AACGTTC	25	0.0021027524	36.0	11
ATGGGCG	75	1.8189894E-12	36.0	5
CCCTACC	25	0.0021027524	36.0	20
CCACTAG	25	0.0021027524	36.0	33
>>END_MODULE