##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936569.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	178300
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85130117779024	31.0	31.0	34.0	30.0	34.0
2	31.974531688166014	33.0	31.0	34.0	30.0	34.0
3	31.8633202467751	33.0	31.0	34.0	30.0	34.0
4	35.64659001682558	37.0	35.0	37.0	33.0	37.0
5	35.75601233875491	37.0	35.0	37.0	35.0	37.0
6	35.73937745372967	37.0	35.0	37.0	35.0	37.0
7	27.079713965227146	35.0	18.0	37.0	0.0	37.0
8	31.330235558048233	36.0	25.0	37.0	17.0	37.0
9	36.02996634885025	39.0	32.0	39.0	32.0	39.0
10	36.57029725182277	38.0	35.0	39.0	32.0	39.0
11	36.95662927650028	39.0	37.0	39.0	33.0	39.0
12	37.093578238923165	39.0	37.0	39.0	34.0	39.0
13	37.09871564778463	39.0	37.0	39.0	33.0	39.0
14	38.202832305103755	40.0	37.0	41.0	33.0	41.0
15	38.278283791362874	40.0	37.0	41.0	34.0	41.0
16	38.21378014582165	40.0	37.0	41.0	34.0	41.0
17	38.148373527762196	40.0	37.0	41.0	33.0	41.0
18	38.081615255187884	40.0	37.0	41.0	33.0	41.0
19	37.98356141334829	40.0	37.0	41.0	33.0	41.0
20	38.01877734155917	40.0	37.0	41.0	34.0	41.0
21	37.94695457094784	40.0	37.0	41.0	33.0	41.0
22	37.966264722378014	40.0	37.0	41.0	33.0	41.0
23	37.908637128435224	40.0	37.0	41.0	33.0	41.0
24	37.90706113292204	40.0	37.0	41.0	33.0	41.0
25	37.82747055524397	39.0	36.0	41.0	33.0	41.0
26	37.72067863151991	39.0	36.0	41.0	33.0	41.0
27	37.62359506449803	39.0	36.0	41.0	33.0	41.0
28	37.636842400448685	39.0	36.0	41.0	33.0	41.0
29	37.645608524957936	40.0	36.0	41.0	33.0	41.0
30	37.57236118900729	40.0	36.0	41.0	33.0	41.0
31	37.45488502523836	39.0	36.0	41.0	33.0	41.0
32	37.34030286034773	39.0	36.0	41.0	32.0	41.0
33	37.312619181155355	39.0	36.0	41.0	32.0	41.0
34	37.2127201346046	39.0	36.0	41.0	32.0	41.0
35	37.13689848569826	39.0	36.0	41.0	32.0	41.0
36	36.97114974761638	39.0	35.0	41.0	31.0	41.0
37	36.856808749298935	39.0	35.0	41.0	31.0	41.0
38	36.72187324733595	39.0	35.0	41.0	30.0	41.0
39	36.688401570386986	39.0	35.0	41.0	30.0	41.0
40	36.504991587212565	39.0	35.0	40.0	30.0	41.0
41	36.44578799775659	39.0	35.0	40.0	30.0	41.0
42	36.37045989904655	39.0	35.0	40.0	30.0	41.0
43	36.32126191811554	39.0	35.0	40.0	30.0	41.0
44	36.06277061132922	39.0	35.0	40.0	29.0	41.0
45	35.92122265844083	39.0	35.0	40.0	28.0	41.0
46	35.718339876612454	38.0	35.0	40.0	27.0	41.0
47	35.54203589455973	38.0	35.0	40.0	27.0	41.0
48	35.49191811553561	38.0	35.0	40.0	26.0	41.0
49	35.316343241727424	38.0	34.0	40.0	26.0	41.0
50	35.04139091418957	38.0	34.0	40.0	24.0	41.0
51	32.63306786315199	36.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	13.0
11	7.0
12	6.0
13	2.0
14	3.0
15	1.0
16	8.0
17	20.0
18	31.0
19	61.0
20	122.0
21	202.0
22	333.0
23	550.0
24	845.0
25	1184.0
26	1657.0
27	1837.0
28	2434.0
29	2817.0
30	3482.0
31	4507.0
32	5842.0
33	7955.0
34	11739.0
35	15332.0
36	17535.0
37	27099.0
38	35185.0
39	37466.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.04879416713404	22.042624789680314	27.65675827257431	12.251822770611328
2	28.989904655075716	24.64834548513741	29.173303421200224	17.188446438586652
3	30.848569826135723	24.15423443634324	28.723499719573752	16.27369601794728
4	27.527762198541783	26.118339876612453	28.150308468872687	18.20358945597308
5	24.970835670218733	30.777902411665732	26.879416713404375	17.37184520471116
6	25.84576556365676	34.22546270330903	25.30005608524958	14.628715647784633
7	63.02523836231071	26.914189568143577	6.113292204150309	3.947279865395401
8	85.18620302860349	3.6404935501962985	6.830061693774538	4.343241727425687
9	79.16040381379698	7.192933258553001	8.986539540100953	4.660123387549075
10	34.61637689287717	37.37240605720695	15.41839596186203	12.59282108805384
11	25.45260796410544	27.22097588334268	29.34997195737521	17.97644419517667
12	24.85361749859787	24.341559169938307	31.644980370162646	19.159842961301177
13	22.887829500841278	27.223219293325858	32.019629837352774	17.86932136848009
14	19.347167694896243	30.236679753224905	30.49691531127314	19.91923724060572
15	17.561974200785194	27.860347728547392	35.5614133482894	19.016264722378015
16	20.38137969713965	27.985417835109367	31.42905215928211	20.204150308468876
17	20.01065619742008	26.800336511497473	29.026920919798094	24.162086371284353
18	21.599551318003364	26.48065058889512	31.74312955692653	20.176668536174986
19	21.402131239484014	28.56309590577678	29.27986539540101	20.75490745933819
20	22.30959057767807	29.052159282108807	30.045989904655073	18.592260235558047
21	21.755468311833987	28.21144139091419	30.819405496354456	19.21368480089736
22	20.777341559169937	25.653954010095344	29.589455973079083	23.979248457655636
23	17.832865956253503	28.4649467190129	30.049355019629836	23.652832305103757
24	19.810992708917556	26.74032529444756	31.87436904094223	21.574312955692655
25	18.775659001682556	28.773976444195178	30.19742007851935	22.252944475602916
26	18.42624789680314	29.519910263600675	30.108244531688165	21.94559730790802
27	19.726864834548515	28.32305103757712	31.080762759394283	20.86932136848009
28	17.696578799775658	27.726864834548515	32.481772293886706	22.09478407178912
29	17.928210880538415	25.523275378575434	34.21985417835109	22.32865956253505
30	20.128435221536737	26.333707234997195	32.94111048794167	20.596747055524396
31	22.03589455973079	27.79753224901851	29.286595625350532	20.87997756590017
32	20.522153673583848	27.04823331463825	30.829500841278744	21.60011217049916
33	20.446999439147504	26.351093662366797	30.675827257431294	22.5260796410544
34	20.34828939988783	25.351093662366797	31.462142456533932	22.838474481211442
35	17.99551318003365	25.0	33.190689848569825	23.813796971396524
36	21.536174985978686	25.06057206954571	31.641615255187887	21.761637689287717
37	20.85249579360628	26.09814918676388	32.482893998878296	20.566461020751543
38	19.352215367358387	26.181155356141332	31.063376332024678	23.403252944475604
39	20.64385866517106	24.756590016825577	32.10880538418396	22.490745933819404
40	21.84913067863152	23.98766124509254	31.319685922602357	22.843522153673586
41	19.143578238923165	24.50252383623107	30.40886146943354	25.94503645541223
42	20.525518788558607	23.907459338194055	31.17722938867078	24.389792484576557
43	21.02692091979809	24.02355580482333	31.111048794167136	23.838474481211442
44	20.431856421761076	24.63320246775098	31.28266965787998	23.652271452607966
45	20.86315199102636	24.23948401570387	30.968031407739765	23.929332585530005
46	22.288278182837914	25.135726303982054	30.450925406618058	22.125070106561974
47	19.116657319125068	25.210880538418394	32.50757150869321	23.16489063376332
48	19.787436904094225	23.840717891194615	32.1491867638811	24.22265844083006
49	20.612450925406616	22.592821088053842	32.666853617498596	24.127874369040942
50	19.728547392035896	22.702187324733593	32.92372406057207	24.64554122265844
51	19.215367358384743	22.53785754346607	30.78238923163208	27.464385866517105
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	153.0
2	215.0
3	525.0
4	835.0
5	612.5
6	390.0
7	357.0
8	324.0
9	325.0
10	326.0
11	324.5
12	323.0
13	334.5
14	346.0
15	380.5
16	415.0
17	416.5
18	418.0
19	427.0
20	436.0
21	565.0
22	694.0
23	815.5
24	937.0
25	1118.0
26	1602.0
27	1905.0
28	2381.0
29	2857.0
30	3328.0
31	3799.0
32	4346.5
33	4894.0
34	5748.5
35	6603.0
36	6945.5
37	7288.0
38	8156.5
39	9025.0
40	10228.0
41	11431.0
42	12664.5
43	13898.0
44	14973.0
45	16048.0
46	17566.0
47	19084.0
48	19877.0
49	20670.0
50	19016.5
51	17363.0
52	14401.5
53	11440.0
54	9602.5
55	7765.0
56	6587.5
57	5410.0
58	4600.0
59	3790.0
60	3355.0
61	2920.0
62	2454.5
63	1989.0
64	1630.0
65	1271.0
66	998.0
67	725.0
68	586.0
69	447.0
70	365.5
71	284.0
72	216.0
73	148.0
74	125.0
75	71.5
76	41.0
77	35.0
78	29.0
79	22.5
80	16.0
81	9.5
82	3.0
83	3.0
84	3.0
85	1.5
86	0.0
87	1.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	178300.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.340437464946724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2381628020107	41.63712843522154
2	10.264310037295282	11.36062815479529
3	4.447056915842387	7.383062254627033
4	2.8457921193449005	6.299495232753785
5	1.7755796983946814	4.913067863151991
6	1.3965461326414788	4.637128435221537
7	1.0489297875790498	4.063376332024678
8	0.7236095346197503	3.2035894559730793
9	0.5888195232690124	2.932697700504767
>10	1.6519377330955085	12.223219293325856
>50	0.01114804605156478	0.3869882220975883
>100	0.008107669855683477	0.9596186203028604
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	403	0.22602355580482333	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	282	0.15816040381379698	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	269	0.15086932136848008	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	234	0.13123948401570387	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	184	0.10319685922602355	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2142456533931576	0.0
2	5.608524957936063E-4	0.0	0.0	0.9921480650588895	0.0
3	5.608524957936063E-4	0.0	0.0	1.3510936623667975	0.0
4	5.608524957936063E-4	0.0	0.0	1.793606281547953	0.0
5	5.608524957936063E-4	0.0	0.0	3.06225462703309	0.0
6	5.608524957936063E-4	0.0	0.0	3.777902411665732	0.0
7	5.608524957936063E-4	0.0	0.0	4.539540100953449	0.0
8	5.608524957936063E-4	0.0	0.0	6.039820527201346	0.0
9	5.608524957936063E-4	0.0	0.0	6.596186203028603	0.0
10	5.608524957936063E-4	0.0	0.0	7.9848569826135725	0.0
11	5.608524957936063E-4	0.0	0.0	9.861469433538979	0.0
12	5.608524957936063E-4	0.0	0.0	11.327537857543467	0.0
13	5.608524957936063E-4	0.0	0.0	11.94223219293326	0.0
14	5.608524957936063E-4	0.0	0.0	12.153112731351655	0.0
15	5.608524957936063E-4	0.0	0.0	12.56758272574313	0.0
16	5.608524957936063E-4	0.0	0.0	13.522153673583848	0.0
17	5.608524957936063E-4	0.0	0.0	14.795288839035333	0.0
18	5.608524957936063E-4	0.0	0.0	16.18003365114975	0.0
19	5.608524957936063E-4	0.0	0.0	16.98429613011778	0.0
20	5.608524957936063E-4	0.0	0.0	17.772293886707796	0.0
21	5.608524957936063E-4	0.0	0.0	18.698822209758834	0.0
22	5.608524957936063E-4	0.0	0.0	19.790802019068984	0.0
23	5.608524957936063E-4	0.0	0.0	20.80257992148065	0.0
24	5.608524957936063E-4	0.0	0.0	21.57375210319686	0.0
25	5.608524957936063E-4	0.0	0.0	22.260796410544028	0.0
26	5.608524957936063E-4	0.0	0.0	22.885586090858105	0.0
27	5.608524957936063E-4	0.0	0.0	23.509814918676387	0.0
28	5.608524957936063E-4	0.0	0.0	24.179472798653954	0.0
29	5.608524957936063E-4	0.0	0.0	24.86483454851374	0.0
30	5.608524957936063E-4	0.0	0.0	25.64105440269209	0.0
31	5.608524957936063E-4	0.0	0.0	26.38754907459338	0.0
32	5.608524957936063E-4	0.0	0.0	27.03757711721817	0.0
33	5.608524957936063E-4	0.0	0.0	27.680314077397643	0.0
34	5.608524957936063E-4	0.0	0.0	28.314077397644418	0.0
35	5.608524957936063E-4	0.0	0.0	29.022994952327537	0.0
36	5.608524957936063E-4	0.0	0.0	29.63600673022995	0.0
37	5.608524957936063E-4	0.0	0.0	30.308468872686483	0.0
38	5.608524957936063E-4	0.0	0.0	30.98934380257992	0.0
39	5.608524957936063E-4	0.0	0.0	31.708356702187324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.017245E-4	45.0	9
GGCGTAG	20	7.017245E-4	45.0	1
AGGACAC	20	7.017245E-4	45.0	7
GTTAGCG	20	7.017245E-4	45.0	1
TTCCGCG	20	7.017245E-4	45.0	1
GCGTTTG	20	7.017245E-4	45.0	1
CGCGACC	20	7.017245E-4	45.0	10
AGGCGAT	45	3.8016879E-10	45.0	7
CGAATAT	35	1.2042256E-7	45.0	14
GCCTATC	20	7.017245E-4	45.0	41
CGAGCGG	20	7.017245E-4	45.0	2
GGCGCGA	20	7.017245E-4	45.0	8
GCTACGA	35	1.2042256E-7	45.0	10
TAGGATC	20	7.017245E-4	45.0	6
AGCTACG	35	1.2042256E-7	45.0	9
TCCGCGG	25	3.8775157E-5	44.999996	2
CTACGGG	30	2.1551605E-6	44.999996	3
TTTTGCG	30	2.1551605E-6	44.999996	1
GCGCTAG	25	3.8775157E-5	44.999996	1
CTAGGGC	65	0.0	41.538464	4
>>END_MODULE