##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936568.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195238
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99930341429435	33.0	31.0	34.0	30.0	34.0
2	32.12930372161157	33.0	31.0	34.0	30.0	34.0
3	31.753019391716776	33.0	31.0	34.0	30.0	34.0
4	35.62506274393304	37.0	35.0	37.0	33.0	37.0
5	35.80787039408312	37.0	35.0	37.0	35.0	37.0
6	35.80318892838484	37.0	35.0	37.0	35.0	37.0
7	26.142963972177547	35.0	0.0	37.0	0.0	37.0
8	30.830693819850644	35.0	19.0	37.0	17.0	37.0
9	35.90148434218749	39.0	32.0	39.0	32.0	39.0
10	36.689076921500934	38.0	35.0	39.0	33.0	39.0
11	37.06750735000359	39.0	37.0	39.0	34.0	39.0
12	37.20995400485561	39.0	37.0	39.0	34.0	39.0
13	37.2125662012518	39.0	37.0	39.0	34.0	39.0
14	38.43533533430992	40.0	38.0	41.0	34.0	41.0
15	38.50962415103617	40.0	38.0	41.0	34.0	41.0
16	38.44895460924615	40.0	38.0	41.0	34.0	41.0
17	38.37594115899569	40.0	38.0	41.0	34.0	41.0
18	38.216192544484166	40.0	37.0	41.0	34.0	41.0
19	38.0371085546871	40.0	37.0	41.0	34.0	41.0
20	38.03402001659513	40.0	37.0	41.0	34.0	41.0
21	37.965339739190114	40.0	36.0	41.0	33.0	41.0
22	37.98033169772278	40.0	36.0	41.0	33.0	41.0
23	37.92333459674858	40.0	36.0	41.0	33.0	41.0
24	37.87520871961401	40.0	36.0	41.0	33.0	41.0
25	37.758622809084294	40.0	36.0	41.0	33.0	41.0
26	37.64679007160491	40.0	36.0	41.0	33.0	41.0
27	37.52678269599156	40.0	36.0	41.0	33.0	41.0
28	37.4989756092564	40.0	36.0	41.0	33.0	41.0
29	37.47040535141724	40.0	36.0	41.0	33.0	41.0
30	37.36088261506469	40.0	35.0	41.0	33.0	41.0
31	37.18367838228214	39.0	35.0	41.0	32.0	41.0
32	36.98721560351981	39.0	35.0	41.0	31.0	41.0
33	36.80271258668907	39.0	35.0	41.0	31.0	41.0
34	36.503001464878764	39.0	35.0	41.0	30.0	41.0
35	36.29432794845266	39.0	35.0	41.0	29.0	41.0
36	36.03384587016872	39.0	35.0	41.0	28.0	41.0
37	35.923196303998196	39.0	35.0	41.0	27.0	41.0
38	35.732214015714156	39.0	35.0	41.0	26.0	41.0
39	35.61431688503263	39.0	35.0	41.0	26.0	41.0
40	35.40870117497618	39.0	35.0	40.0	24.0	41.0
41	35.24864012128786	39.0	35.0	40.0	24.0	41.0
42	35.12127762013542	38.0	34.0	40.0	23.0	41.0
43	35.12088835165285	38.0	34.0	40.0	23.0	41.0
44	34.94050338561141	38.0	34.0	40.0	23.0	41.0
45	34.82095698583267	38.0	34.0	40.0	23.0	41.0
46	34.634538358311396	38.0	34.0	40.0	22.0	41.0
47	34.47139901043854	38.0	34.0	40.0	21.0	41.0
48	34.407917516057324	38.0	34.0	40.0	21.0	41.0
49	34.26694598387609	38.0	33.0	40.0	20.0	41.0
50	34.0673127157623	38.0	33.0	40.0	20.0	41.0
51	32.047956852661876	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	17.0
10	20.0
11	9.0
12	9.0
13	7.0
14	9.0
15	9.0
16	26.0
17	42.0
18	81.0
19	153.0
20	256.0
21	369.0
22	668.0
23	1055.0
24	1644.0
25	2335.0
26	3113.0
27	3414.0
28	3477.0
29	3695.0
30	4139.0
31	4847.0
32	6257.0
33	7984.0
34	12056.0
35	15278.0
36	17968.0
37	28947.0
38	37175.0
39	40165.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.131552259293784	22.62879152623977	25.796719900838976	19.44293631362747
2	32.061893688728624	26.109159077638573	24.467572911011175	17.36137432262162
3	29.27452647537877	24.198158145442996	29.993136582017843	16.53417879716039
4	26.636208115223468	25.006402442147536	30.07611223224987	18.281277210379127
5	25.600036878066767	27.61296468925107	27.942306313320152	18.84469211936201
6	26.467183642528607	32.45218656204222	27.17094008338541	13.909689712043763
7	59.23846792120386	29.081428820209183	8.519857814564787	3.160245444022168
8	84.01540683678382	4.0202214732787676	9.504297319169424	2.4600743707679857
9	77.62474518280253	6.882881406283612	11.191468873887256	4.300904537026604
10	35.89465165592764	34.67409008492199	17.43922801913562	11.992030240014753
11	26.656695930095577	25.152890318483085	31.549186121554207	16.641227629867135
12	25.814646738852066	22.649791536483676	32.75899158975199	18.77657013491226
13	22.717401325561625	24.055255636710065	34.33501674878866	18.892326288939653
14	20.203034245382558	26.215183519601716	33.03967465349983	20.542107581515893
15	18.907692150093734	26.006207807906247	35.55199295219168	19.534107089808337
16	22.64876714574007	26.16908593613948	32.84094284924041	18.341204068880035
17	23.286962579006136	24.944426802159416	31.337649432999726	20.430961185834725
18	24.081889796043804	24.275499646585192	32.08647906657515	19.55613149079585
19	22.77732818406253	26.658744711582788	30.618014935617044	19.945912168737642
20	23.988670238375725	26.196744486216822	31.404234831334065	18.410350444073387
21	24.156158124955184	25.62718323277231	32.1530644649095	18.06359417736301
22	23.757670125692744	23.300791854044807	31.5870885790676	21.354449441194852
23	21.441010459029492	25.88737848164804	32.307747467193884	20.36386359212858
24	21.349839682848625	25.766500373902623	32.944918509716345	19.938741433532407
25	21.70223009864883	27.10589127116647	30.851576025159037	20.340302605025663
26	21.158278613794444	27.386062139542506	30.138087872237985	21.31757137442506
27	20.560034419528986	27.063379055306857	31.697722779376964	20.678863745787194
28	19.519765619397862	26.638256896710683	32.74823548694414	21.093741996947315
29	20.22864401397269	25.573914914104833	32.37535725627183	21.822083815650643
30	21.479425111914686	25.896597998340486	31.81347893340436	20.810497956340466
31	21.849742365727984	26.505086100042003	30.124258597199315	21.5209129370307
32	22.01979122916645	26.58703735953042	30.264600129073234	21.128571282229895
33	21.833864309202102	26.28740306702589	29.350331390405554	22.528401233366456
34	20.64710763273543	26.967598520779767	31.22035669285692	21.16493715362788
35	20.91037605384198	25.991354142123974	30.87001505854393	22.22825474549012
36	21.621815425275816	27.911574591011995	29.58338028457575	20.88322969913644
37	21.371351888464336	27.310257224515723	29.931673137401532	21.386717749618413
38	21.108083467357787	27.463915836056508	29.65662422274352	21.77137647384218
39	21.82362040176605	26.429281185015213	29.016892203362048	22.73020620985669
40	23.19784058431248	25.97598828096989	30.087380530429524	20.7387906042881
41	21.320644546655878	26.609573955889736	30.426453866562863	21.643327630891527
42	21.302717708642785	26.947622901279466	30.12374640182751	21.62591298825024
43	21.883547260266955	26.679232526454893	29.572111986396095	21.865108226882064
44	21.877400915805325	26.8380130917137	28.820721375961643	22.463864616519324
45	21.69864473104621	26.33093967362911	28.472428523135868	23.497987072188817
46	22.03413269957693	27.284647455925587	28.11440395824583	22.56681588625165
47	20.612278347452854	26.84774480377795	30.7855028221965	21.754474026572694
48	20.878619940790216	26.793452094366877	30.487917311179174	21.840010653663732
49	21.426668988619017	25.80952478513404	30.113502494391458	22.650303731855477
50	20.930863868714084	26.01491512922689	30.57755150124464	22.476669500814392
51	20.818693082289307	26.043598070047842	29.256599637365678	23.881109210297176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	206.5
2	352.0
3	1873.0
4	3394.0
5	2365.5
6	1337.0
7	1205.0
8	1073.0
9	995.0
10	917.0
11	933.5
12	950.0
13	906.0
14	862.0
15	858.0
16	854.0
17	810.5
18	767.0
19	814.0
20	861.0
21	947.5
22	1034.0
23	1126.5
24	1219.0
25	1415.0
26	1948.0
27	2285.0
28	2750.0
29	3215.0
30	3587.5
31	3960.0
32	4838.5
33	5717.0
34	6321.0
35	6925.0
36	7424.5
37	7924.0
38	8412.5
39	8901.0
40	9744.0
41	10587.0
42	11367.0
43	12147.0
44	12937.0
45	13727.0
46	14535.5
47	15344.0
48	15470.5
49	15597.0
50	14834.5
51	14072.0
52	13116.0
53	12160.0
54	11143.5
55	10127.0
56	9303.0
57	8479.0
58	7717.0
59	6955.0
60	6465.0
61	5975.0
62	5456.5
63	4938.0
64	4259.0
65	3580.0
66	3075.0
67	2570.0
68	2084.5
69	1599.0
70	1347.5
71	1096.0
72	981.0
73	866.0
74	710.5
75	444.5
76	334.0
77	234.0
78	134.0
79	103.5
80	73.0
81	62.0
82	51.0
83	36.5
84	22.0
85	19.5
86	17.0
87	9.0
88	1.0
89	4.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	195238.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.06143830653184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.8885352845628	35.864362297446085
2	12.065918604472916	12.563381540768031
3	6.624610761912443	10.346602934582746
4	4.051991204480162	8.438099604426144
5	2.7529820110260816	7.166210156301315
6	1.8129877874898777	5.663205110933996
7	1.1889919797119455	4.333044281911422
8	0.812994515634135	3.3860531055388208
9	0.5939959929607023	2.7831857167665652
>10	1.1969919252075512	7.850291324931358
>50	0.002999979762424892	0.1192198894760434
>100	0.005999959524849784	0.6106765517703008
>500	0.0	0.0
>1k	9.999932541416307E-4	0.8756674851471833
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1682	0.8615126153720075	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	288	0.14751226707915469	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.14187811798932584	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021000010243907435	0.0
2	0.0	0.0	0.0	0.1557073930279966	0.0
3	0.0	0.0	0.0	0.2484147553242709	0.0
4	0.0	0.0	0.0	0.3959270224034256	0.0
5	0.0	0.0	0.0	0.906585808090638	0.0
6	0.0	0.0	0.0	1.2758786711603274	0.0
7	0.0	0.0	0.0	1.5652690562288079	0.0
8	0.0	0.0	0.0	2.11690347166023	0.0
9	0.0	0.0	0.0	2.385806041856606	0.0
10	0.0	0.0	0.0	3.043977094622973	0.0
11	0.0	0.0	0.0	3.6007334637724213	0.0
12	0.0	0.0	0.0	4.217928886794579	0.0
13	0.0	0.0	0.0	4.447392413362153	0.0
14	0.0	0.0	0.0	4.54470953400465	0.0
15	0.0	0.0	0.0	4.717319374302134	0.0
16	0.0	0.0	0.0	4.986221944498509	0.0
17	0.0	0.0	0.0	5.322222108401029	0.0
18	0.0	0.0	0.0	5.685368627009086	0.0
19	0.0	0.0	0.0	5.897417510935371	0.0
20	0.0	0.0	0.0	6.143783484772432	0.0
21	0.0	0.0	0.0	6.433173869840912	0.0
22	0.0	0.0	0.0	6.7640520800254045	0.0
23	0.0	0.0	0.0	7.0626619817863325	0.0
24	0.0	0.0	0.0	7.2992962435591435	0.0
25	0.0	0.0	0.0	7.521076839549678	0.0
26	0.0	0.0	0.0	7.721857425296305	0.0
27	0.0	0.0	0.0	7.960540468556326	0.0
28	0.0	0.0	0.0	8.191540581239307	0.0
29	0.0	0.0	0.0	8.441491922678987	0.0
30	0.0	0.0	0.0	8.75290670873498	0.0
31	0.0	0.0	0.0	9.029492209508394	0.0
32	0.0	0.0	0.0	9.284053309294297	0.0
33	0.0	0.0	0.0	9.546809535029041	0.0
34	0.0	0.0	0.0	9.816736495969023	0.0
35	0.0	0.0	0.0	10.112273225499134	0.0
36	0.0	0.0	0.0	10.380663600323707	0.0
37	0.0	0.0	0.0	10.659297882584333	0.0
38	0.0	0.0	0.0	10.945615095421998	0.0
39	0.0	0.0	0.0	11.344103094684437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.2050077E-7	45.0	21
TAAAGCG	20	7.0188876E-4	45.0	1
GGATCGA	20	7.0188876E-4	45.0	8
TATGCGG	25	3.878875E-5	45.0	2
CGTTTTA	140	0.0	45.0	1
ACACGAC	30	2.1562191E-6	44.999996	26
CGTTTTT	840	0.0	43.125	1
AGCGACT	90	0.0	42.5	19
CACAACG	90	0.0	42.5	12
TACGGCT	40	3.4385994E-7	39.375	7
ATGCATA	40	3.4385994E-7	39.375	26
GAGCGAC	105	0.0	38.57143	18
TGCGGGC	35	6.221677E-6	38.571426	4
AGACACG	35	6.221677E-6	38.571426	24
GTGAAAC	35	6.221677E-6	38.571426	37
AACGAGC	100	0.0	38.25	15
GGCGATA	65	9.094947E-12	38.076927	8
TCGCAGG	30	1.13645365E-4	37.499996	2
TTGCCGG	30	1.13645365E-4	37.499996	2
CACCGGG	30	1.13645365E-4	37.499996	3
>>END_MODULE