##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936566.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189188
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.993012241791234	33.0	31.0	34.0	30.0	34.0
2	32.11044569423008	33.0	31.0	34.0	30.0	34.0
3	31.97745628686809	33.0	31.0	34.0	30.0	34.0
4	35.73790620969618	37.0	35.0	37.0	35.0	37.0
5	35.83747383554982	37.0	35.0	37.0	35.0	37.0
6	35.823704463285196	37.0	35.0	37.0	35.0	37.0
7	26.905977123284774	35.0	11.0	37.0	0.0	37.0
8	31.211525043871703	36.0	19.0	37.0	17.0	37.0
9	35.978164577034484	39.0	32.0	39.0	32.0	39.0
10	36.62545721715965	38.0	35.0	39.0	32.0	39.0
11	36.98523162145591	39.0	37.0	39.0	34.0	39.0
12	37.04404613400427	39.0	37.0	39.0	34.0	39.0
13	37.00533860498552	39.0	37.0	39.0	33.0	39.0
14	38.18868532887921	40.0	37.0	41.0	33.0	41.0
15	38.26213607628391	40.0	37.0	41.0	34.0	41.0
16	38.22314311689959	40.0	37.0	41.0	34.0	41.0
17	38.1053819481151	40.0	37.0	41.0	33.0	41.0
18	38.01088335412394	40.0	37.0	41.0	33.0	41.0
19	37.91448189102903	40.0	37.0	41.0	33.0	41.0
20	37.96498192274351	40.0	36.0	41.0	34.0	41.0
21	37.91100915491469	40.0	36.0	41.0	33.0	41.0
22	37.91297545298856	40.0	36.0	41.0	33.0	41.0
23	37.88562170962217	40.0	36.0	41.0	33.0	41.0
24	37.848896335919825	40.0	36.0	41.0	33.0	41.0
25	37.76210436179885	40.0	36.0	41.0	33.0	41.0
26	37.65031608770112	40.0	36.0	41.0	33.0	41.0
27	37.553164048459735	40.0	36.0	41.0	33.0	41.0
28	37.527174027951034	40.0	36.0	41.0	33.0	41.0
29	37.50483117322452	40.0	36.0	41.0	33.0	41.0
30	37.41557075501618	39.0	36.0	41.0	33.0	41.0
31	37.31630970251813	39.0	35.0	41.0	32.0	41.0
32	37.200033828784065	39.0	35.0	41.0	32.0	41.0
33	37.0870562614965	39.0	35.0	41.0	32.0	41.0
34	36.941433917584625	39.0	35.0	41.0	31.0	41.0
35	36.856962386620715	39.0	35.0	41.0	31.0	41.0
36	36.720251813011394	39.0	35.0	41.0	31.0	41.0
37	36.649496796837006	39.0	35.0	41.0	30.0	41.0
38	36.565500983149036	39.0	35.0	41.0	30.0	41.0
39	36.50624775355731	39.0	35.0	41.0	30.0	41.0
40	36.346491320802585	39.0	35.0	41.0	30.0	41.0
41	36.294194134934564	39.0	35.0	40.0	30.0	41.0
42	36.20108040679113	39.0	35.0	40.0	30.0	41.0
43	36.15914857179102	39.0	35.0	40.0	30.0	41.0
44	35.95367042307123	38.0	35.0	40.0	29.0	41.0
45	35.84665517897541	38.0	35.0	40.0	29.0	41.0
46	35.681729285155505	38.0	35.0	40.0	28.0	41.0
47	35.56962386620716	38.0	35.0	40.0	27.0	41.0
48	35.494925682390004	38.0	35.0	40.0	27.0	41.0
49	35.34781275768019	38.0	34.0	40.0	27.0	41.0
50	35.133718840518426	38.0	34.0	40.0	26.0	41.0
51	32.93094170877646	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	11.0
10	18.0
11	11.0
12	8.0
13	5.0
14	12.0
15	14.0
16	21.0
17	32.0
18	73.0
19	119.0
20	181.0
21	314.0
22	468.0
23	638.0
24	958.0
25	1320.0
26	1779.0
27	2013.0
28	2478.0
29	2939.0
30	3644.0
31	4703.0
32	6011.0
33	8506.0
34	13836.0
35	16820.0
36	17586.0
37	27841.0
38	36571.0
39	40242.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66120472757257	20.221155675835675	24.939742478381294	13.177897118210458
2	31.24141066029558	25.239972936972748	26.10894982768463	17.40966657504704
3	30.850265344525024	24.654840687570037	27.906103981225023	16.588789986679917
4	27.770260270207416	25.167029621329046	28.236991775376875	18.825718333086666
5	24.514768378544094	29.645643486901918	26.06719242235237	19.77239571220162
6	25.701418694631794	34.67926084106814	25.479417299194452	14.139903165105608
7	61.65930185846883	27.611687844895027	6.684884876419223	4.044125420216927
8	83.1437511892932	5.088589128274521	7.489904222255111	4.277755460177178
9	77.20891388460156	6.97401526523881	9.781275768019112	6.035795082140516
10	41.45823202317272	26.130621392477327	17.950398545362283	14.460748038987672
11	30.5389348161617	25.636404000253716	25.903334249529568	17.921326934055017
12	30.59496373977208	21.844937311034528	27.79774615726156	19.762352791931832
13	24.388967587796266	25.546017717825652	29.031968200942977	21.0330464934351
14	19.336321542592554	28.404021396705918	29.651986383914412	22.607670676787112
15	19.001733725183414	26.196693236357486	34.834661817874284	19.966911220584816
16	21.05313233397467	25.039114531577056	32.49571854451656	21.412034589931707
17	21.171004503456878	25.134786561515533	29.841744719538237	23.852464215489356
18	21.933737869209462	26.18559316658562	30.859779690043766	21.020889274161153
19	23.67433452438844	28.04036196798951	27.0212698479819	21.26403365964015
20	24.52533987356492	26.769139691735205	28.98386789859822	19.721652536101654
21	22.609256401040234	28.760809353658797	28.083705097574903	20.546229147726073
22	21.982366746305264	25.737890352453647	27.837389263589657	24.442353637651436
23	22.025181301139607	26.597352897646786	28.286677801974758	23.090787999238852
24	21.79313698543248	24.763198511533503	30.513563228111718	22.9301012749223
25	20.400871091189714	27.575744761824218	28.563650971520392	23.459733175465676
26	19.190963486056198	28.08899084508531	29.09063999830856	23.62940567054993
27	19.722709685603736	29.086939975051273	28.048819164006172	23.141531175338816
28	18.805632492547094	28.360678267120537	30.564306404211685	22.269382836120684
29	20.63555828065205	24.97145696344377	30.86929403556251	23.52369072034167
30	20.728058862084275	27.55618749603569	28.95479628729095	22.760957354589088
31	22.152567816140557	28.703194705795294	26.793982705034146	22.350254773030002
32	23.3064464976637	27.387572150453515	27.186185170306786	22.119796181576
33	22.081738799501025	27.311457386303573	26.73953950567689	23.86726430851851
34	20.249170137640863	27.195170941074487	29.661500729433154	22.894158191851492
35	19.40450768547688	27.1031989343933	29.238112353849083	24.254181026280737
36	21.861323128316805	28.858595682601436	26.450409116857305	22.829672072224454
37	20.824788041524833	29.01346808465653	29.417299194452077	20.744444679366556
38	20.335327822060595	27.139142017464106	28.204220140812314	24.32131001966298
39	20.49707169587923	26.53762395077912	28.746537835380682	24.218766517960972
40	21.357591390574456	25.430259847347614	29.22965515783242	23.982493604245512
41	18.552445186798316	26.24056494069391	29.045182569719007	26.16180730278876
42	20.330042074550185	25.5555320633444	28.870224327124344	25.244201534981077
43	20.8358881112967	25.755333319238005	28.230120303613337	25.178658265851954
44	20.9268029684758	25.226758568196715	28.203162991310233	25.64327547201725
45	19.868596316891136	26.756982472461253	28.26342051292894	25.111000697718673
46	20.067340423282662	28.310463665771614	27.38440070194727	24.237795208998456
47	19.414550605746665	25.904919973782693	30.449605683235724	24.230923737234917
48	19.98012558936085	25.027485887054148	30.187961181470286	24.80442734211472
49	20.620758187622894	25.04545742858955	29.386641858891682	24.94714252489587
50	19.198892107321818	24.81869886039284	30.216504218026515	25.765904814258832
51	18.51333065522126	25.211429900416515	28.25179186840603	28.02344757595619
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	75.0
1	223.0
2	371.0
3	854.5
4	1338.0
5	925.0
6	512.0
7	466.5
8	421.0
9	425.0
10	429.0
11	402.0
12	375.0
13	386.5
14	398.0
15	434.5
16	471.0
17	443.0
18	415.0
19	443.0
20	471.0
21	491.5
22	512.0
23	631.5
24	751.0
25	952.0
26	1534.0
27	1915.0
28	2406.0
29	2897.0
30	3161.0
31	3425.0
32	4106.5
33	4788.0
34	5455.0
35	6122.0
36	6686.5
37	7251.0
38	7750.5
39	8250.0
40	9194.0
41	10138.0
42	11055.0
43	11972.0
44	13080.5
45	14189.0
46	15965.5
47	17742.0
48	17634.5
49	17527.0
50	16793.0
51	16059.0
52	14214.0
53	12369.0
54	11104.0
55	9839.0
56	8930.5
57	8022.0
58	7549.5
59	7077.0
60	6679.5
61	6282.0
62	5833.0
63	5384.0
64	4533.5
65	3683.0
66	3010.5
67	2338.0
68	1952.5
69	1567.0
70	1241.0
71	915.0
72	814.5
73	714.0
74	563.5
75	324.0
76	235.0
77	222.5
78	210.0
79	139.0
80	68.0
81	54.0
82	40.0
83	31.0
84	22.0
85	16.0
86	10.0
87	8.0
88	6.0
89	5.0
90	4.0
91	7.0
92	10.0
93	8.0
94	6.0
95	6.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	189188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46250290716113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3826582450848	36.52610102120642
2	9.913165530172098	10.00486288770958
3	5.105322146455918	7.7288200097257755
4	3.213608605934911	6.486669344778738
5	2.340026605494977	5.904179969131235
6	1.7513538426085953	5.302661902446244
7	1.2852339502875278	4.539928536693659
8	1.0767893242832753	4.346998752563588
9	0.7667410363573516	3.4822504598600332
>10	2.1378667420838178	13.658371566906993
>50	0.015711906482732615	0.5560606380954395
>100	0.010474604321821743	1.1163498741992093
>500	0.0010474604321821742	0.3467450366830877
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.3467450366830877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	450	0.2378586379685815	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	299	0.15804385056134637	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	275	0.14535805653635536	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	239	0.12632936549886883	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	201	0.10624352495929973	Illumina PCR Primer Index 3 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	194	0.1025435017020107	RNA PCR Primer, Index 7 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10148635219992812	0.0
2	0.0	0.0	0.0	0.5143032327631774	0.0
3	0.0	0.0	0.0	0.6919043491130515	0.0
4	0.0	0.0	0.0	0.9271201133264266	0.0
5	0.0	0.0	0.0	1.7479967016935536	0.0
6	0.0	0.0	0.0	2.253314163689029	0.0
7	0.0	0.0	0.0	2.7644459479459584	0.0
8	0.0	0.0	0.0	3.834809818804575	0.0
9	0.0	0.0	0.0	4.299955599720913	0.0
10	0.0	0.0	0.0	5.624563925830391	0.0
11	0.0	0.0	0.0	6.938600756919043	0.0
12	0.0	0.0	0.0	8.088250840433854	0.0
13	0.0	0.0	0.0	8.506882043258557	0.0
14	0.0	0.0	0.0	8.66281159481574	0.0
15	0.0	0.0	0.0	8.976784996934267	0.0
16	0.0	0.0	0.0	9.635917711482758	0.0
17	0.0	0.0	0.0	10.416622618770747	0.0
18	0.0	0.0	0.0	11.302513901515953	0.0
19	0.0	0.0	0.0	11.765016808677084	0.0
20	0.0	0.0	0.0	12.229105440091338	0.0
21	0.0	0.0	0.0	12.840666427046113	0.0
22	0.0	0.0	0.0	13.504027739602934	0.0
23	0.0	0.0	0.0	14.165803327906632	0.0
24	0.0	0.0	0.0	14.668477916146902	0.0
25	0.0	0.0	0.0	15.137323720320527	0.0
26	0.0	0.0	0.0	15.544854853373364	0.0
27	0.0	0.0	0.0	15.953443135928284	0.0
28	0.0	0.0	0.0	16.380002960018604	0.0
29	0.0	0.0	0.0	16.79123411632873	0.0
30	0.0	0.0	0.0	17.312408820855445	0.0
31	0.0	0.0	0.0	17.78072605027803	0.0
32	0.0	0.0	0.0	18.211514472376685	0.0
33	0.0	0.0	0.0	18.625388502442014	0.0
34	0.0	0.0	0.0	19.069391293316702	0.0
35	5.285747510412923E-4	0.0	0.0	19.560965811785103	0.0
36	5.285747510412923E-4	0.0	0.0	20.015011522929573	0.0
37	5.285747510412923E-4	0.0	0.0	20.493371672621944	0.0
38	5.285747510412923E-4	0.0	0.0	20.959574603040362	0.0
39	5.285747510412923E-4	0.0	0.0	21.439520476985855	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTC	20	7.018332E-4	45.000004	31
GGATGCG	20	7.018332E-4	45.000004	8
AAGACGG	20	7.018332E-4	45.000004	2
TATGCGG	20	7.018332E-4	45.000004	2
CCACTGG	20	7.018332E-4	45.000004	2
TCGGACG	20	7.018332E-4	45.000004	1
CGCTCGT	20	7.018332E-4	45.000004	42
GACGAGG	20	7.018332E-4	45.000004	2
TATACTG	35	1.2047349E-7	45.000004	18
TAGTCCT	20	7.018332E-4	45.000004	27
ATCTACC	45	3.8198777E-10	45.0	15
TTTACGG	25	3.8784157E-5	45.0	2
TTACTAT	25	3.8784157E-5	45.0	15
AATGGGT	50	2.1827873E-11	45.0	4
CAAGGCG	25	3.8784157E-5	45.0	5
TCAACGG	25	3.8784157E-5	45.0	2
AGACCTA	30	2.1558608E-6	44.999996	32
GACCTAT	30	2.1558608E-6	44.999996	33
CTACCTT	60	3.6379788E-12	41.249996	17
AATATAC	55	6.002665E-11	40.909092	16
>>END_MODULE