##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936565.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	75778
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.874699780939057	31.0	31.0	34.0	30.0	34.0
2	31.993362189553697	33.0	31.0	34.0	30.0	34.0
3	31.87279949325662	33.0	31.0	34.0	30.0	34.0
4	35.67089392699728	37.0	35.0	37.0	33.0	37.0
5	35.75353004829898	37.0	35.0	37.0	35.0	37.0
6	35.73435561772546	37.0	35.0	37.0	35.0	37.0
7	26.85728047718335	35.0	11.0	37.0	0.0	37.0
8	31.171197445168783	35.0	19.0	37.0	17.0	37.0
9	35.90984190662198	39.0	32.0	39.0	32.0	39.0
10	36.6614320779118	38.0	35.0	39.0	33.0	39.0
11	36.98280503576236	39.0	37.0	39.0	34.0	39.0
12	36.88136398426984	39.0	35.0	39.0	33.0	39.0
13	36.66616960067566	39.0	35.0	39.0	33.0	39.0
14	37.67400828736572	40.0	36.0	41.0	33.0	41.0
15	37.85854733563831	40.0	36.0	41.0	33.0	41.0
16	37.81589643432131	40.0	36.0	41.0	33.0	41.0
17	37.74899047216871	40.0	36.0	41.0	33.0	41.0
18	37.672873393333155	39.0	36.0	41.0	33.0	41.0
19	37.59303491778616	39.0	36.0	41.0	33.0	41.0
20	37.6275436142416	39.0	35.0	41.0	33.0	41.0
21	37.54936789041675	39.0	35.0	41.0	33.0	41.0
22	37.53242365858165	39.0	35.0	41.0	33.0	41.0
23	37.46446198104991	39.0	35.0	41.0	33.0	41.0
24	37.42222016944232	39.0	35.0	41.0	33.0	41.0
25	37.31428646836813	39.0	35.0	41.0	33.0	41.0
26	37.13785003563039	39.0	35.0	41.0	32.0	41.0
27	37.02977117369157	39.0	35.0	41.0	32.0	41.0
28	37.00560848795165	39.0	35.0	41.0	32.0	41.0
29	37.03399403520811	39.0	35.0	41.0	32.0	41.0
30	36.967959038243286	39.0	35.0	41.0	32.0	41.0
31	36.79977038190504	39.0	35.0	41.0	31.0	41.0
32	36.625682915885875	39.0	35.0	40.0	31.0	41.0
33	36.49378447570535	38.0	35.0	40.0	31.0	41.0
34	36.37860592784185	38.0	35.0	40.0	30.0	41.0
35	36.24548021853308	38.0	35.0	40.0	30.0	41.0
36	36.10435746522737	38.0	35.0	40.0	30.0	41.0
37	36.00843252659083	38.0	35.0	40.0	30.0	41.0
38	35.873010636332445	38.0	35.0	40.0	29.0	41.0
39	35.90633165298635	38.0	35.0	40.0	30.0	41.0
40	35.73087175697432	38.0	35.0	40.0	29.0	41.0
41	35.69414605822271	38.0	35.0	40.0	29.0	41.0
42	35.59708622555359	38.0	35.0	40.0	28.0	41.0
43	35.58960384280398	38.0	35.0	40.0	28.0	41.0
44	35.36895932856502	38.0	34.0	40.0	27.0	41.0
45	35.26468104199108	38.0	34.0	40.0	27.0	41.0
46	35.12008762437647	38.0	34.0	40.0	26.0	41.0
47	35.04796906753939	37.0	34.0	40.0	26.0	41.0
48	34.97096782707382	37.0	34.0	40.0	26.0	41.0
49	34.85230541845918	37.0	34.0	40.0	26.0	41.0
50	34.57181503866558	37.0	34.0	40.0	24.0	41.0
51	32.40631845654411	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	6.0
11	7.0
12	5.0
13	0.0
14	2.0
15	5.0
16	9.0
17	16.0
18	31.0
19	53.0
20	106.0
21	147.0
22	256.0
23	346.0
24	495.0
25	650.0
26	823.0
27	929.0
28	1117.0
29	1303.0
30	1617.0
31	2048.0
32	2755.0
33	3896.0
34	6637.0
35	8228.0
36	7141.0
37	10732.0
38	13181.0
39	13229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.758821821636886	21.16841299585632	24.31312518145108	12.759640001055716
2	31.509145134471744	24.462244978753727	25.570746126844202	18.457863759930323
3	32.55826229248594	23.985853413919607	27.50270527065903	15.953179022935416
4	29.94800601757766	25.879542875240837	26.353295151627123	17.819155955554383
5	24.255060835598723	30.164427670300086	25.719865924146852	19.86064556995434
6	26.300509382670434	33.58626514291747	25.223679695953972	14.889545778458126
7	59.79439942991369	28.178363113304655	7.322705798516719	4.704531658264932
8	80.40460291905302	5.340600174193037	9.715220776478661	4.539576130275278
9	74.1692839610441	8.293963947319803	11.496740478766924	6.04001161286917
10	44.20544221277943	25.77793026999921	17.609332523951544	12.407294993269815
11	36.902531077621475	23.25213122542163	23.054184591834044	16.79115310512286
12	32.130697563936764	22.631898440180528	27.576605347198395	17.660798648684313
13	23.773390693868933	29.092876560479297	29.72366649951173	17.41006624614004
14	19.913431338911032	31.478793317321653	29.754018316661828	18.85375702710549
15	19.20874132333923	25.39127451239146	37.308981498587976	18.091002665681334
16	20.587769537332733	23.776029982316768	34.974530866478396	20.6616696138721
17	21.02457177544934	22.985563092190343	29.7289450764074	26.260920055952912
18	23.876322943334475	24.21943044155296	31.111932223072657	20.792314392039906
19	25.238195782417062	25.12866531183193	28.269418564754943	21.363720340996068
20	26.82044920689382	24.8198685634353	27.977777191269233	20.381905038401644
21	24.03336060598063	26.919422523687615	29.410910818443348	19.63630605188841
22	22.308585605320804	24.664150545013065	27.76927340389031	25.257990445775818
23	21.74773681015598	26.416638074375147	29.01897648393993	22.816648631528942
24	22.74010926654174	23.85124970308005	31.74008287365726	21.668558156720948
25	19.70360790730819	26.23452717147457	30.102404391775977	23.959460529441262
26	18.584549605426375	28.558420649792815	29.699912903481223	23.157116841299587
27	21.42310433107234	27.662382221753017	29.16809628124258	21.746417165932066
28	17.88117923407849	27.642587558394254	33.028055636200484	21.44817757132677
29	20.139090501200876	25.82543746206023	31.53025944205442	22.505212594684473
30	22.146269365778988	25.404470954630632	29.892580960173138	22.556678719417246
31	25.19596716725171	26.229248594578902	26.765024149489296	21.80976008868009
32	25.967959038243286	27.911794980073374	25.804323154477554	20.315922827205785
33	24.574414737786693	25.97719654781071	26.528807833408113	22.919580880994484
34	20.874132333922773	26.256961123281165	29.585103856000423	23.28380268679564
35	20.560057008630473	27.613555385468082	28.001530787299743	23.824856818601706
36	23.220459764047614	26.92470110058328	28.624402860988678	21.230436274380427
37	23.10697036079073	27.401092665417405	28.53202776531447	20.959909208477395
38	21.731901079468976	28.529388476866636	26.64097759244108	23.09773285122331
39	22.70711816094381	26.421916651270816	27.182031724247143	23.68893346353823
40	22.627939507508778	25.550951463485443	27.498746337987278	24.3223626910185
41	19.858006281506505	26.560479294782123	27.275726464145265	26.3057879595661
42	21.854627992293278	25.441420992900316	28.624402860988678	24.079548153817733
43	23.447438570561378	24.16532502837235	28.276016785874532	24.111219615191747
44	21.190846947662912	25.160336773205945	28.94243711895273	24.706379160178415
45	22.049935337433027	26.24376468104199	26.47206313177967	25.234236849745308
46	22.667528834226292	26.51957032384069	27.160917416664464	23.651983425268547
47	20.120615482066036	25.804323154477554	29.920293488875398	24.15476787458101
48	21.16841299585632	23.35506347488717	30.561640581699173	24.914882947557338
49	20.872812689698858	22.54348227717807	32.03964211248647	24.5440629206366
50	19.64026498456016	25.248752936208398	29.893900604397057	25.217081474834384
51	19.669297157486344	25.429544194885057	27.748159096307635	27.152999551320967
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	149.5
2	230.0
3	362.0
4	494.0
5	352.5
6	211.0
7	200.5
8	190.0
9	196.5
10	203.0
11	214.5
12	226.0
13	227.5
14	229.0
15	243.0
16	257.0
17	266.0
18	275.0
19	271.0
20	267.0
21	255.0
22	243.0
23	253.5
24	264.0
25	319.5
26	508.0
27	641.0
28	747.5
29	854.0
30	1009.5
31	1165.0
32	1285.5
33	1406.0
34	1718.5
35	2031.0
36	2042.5
37	2054.0
38	2442.0
39	2830.0
40	3210.0
41	3590.0
42	4096.5
43	4603.0
44	5462.5
45	6322.0
46	6797.5
47	7273.0
48	7547.0
49	7821.0
50	7372.0
51	6923.0
52	6226.0
53	5529.0
54	4840.5
55	4152.0
56	3781.0
57	3410.0
58	3237.0
59	3064.0
60	2791.5
61	2519.0
62	2281.5
63	2044.0
64	1721.0
65	1398.0
66	1160.5
67	923.0
68	733.5
69	544.0
70	511.0
71	478.0
72	380.5
73	283.0
74	238.0
75	133.5
76	74.0
77	67.5
78	61.0
79	44.0
80	27.0
81	17.5
82	8.0
83	8.0
84	8.0
85	7.0
86	6.0
87	6.5
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	75778.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.31832457969332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91966673523966	37.42115125762094
2	9.632791606665295	9.886774525587901
3	5.004114379757252	7.704082979228801
4	3.137214564904341	6.439863812716092
5	2.494342727833779	6.400274485998575
6	1.6971816498662826	5.225791126712238
7	1.200884591647809	4.313916967985431
8	1.085167660975108	4.4551188999445746
9	0.7585887677432627	3.5036554145002508
>10	2.0520469039292326	13.708464198052205
>50	0.007714462044846739	0.2322573834094328
>100	0.010285949393128985	0.7086489482435535
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	192	0.25337169099210854	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	121	0.15967695109398505	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT	116	0.1530787299743989	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	108	0.14252157618306105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19266805669191586	0.0
2	0.0	0.0	0.0	0.836654437963525	0.0
3	0.0	0.0	0.0	1.1718440708385018	0.0
4	0.0	0.0	0.0	1.50175512681781	0.0
5	0.0	0.0	0.0	2.5363561983689196	0.0
6	0.0	0.0	0.0	3.0483781572488056	0.0
7	0.0	0.0	0.0	3.597350154398374	0.0
8	0.0	0.0	0.0	4.711129879384518	0.0
9	0.0	0.0	0.0	5.129457098366281	0.0
10	0.0	0.0	0.0	6.207606429306659	0.0
11	0.0	0.0	0.0	7.598511441315422	0.0
12	0.0	0.0	0.0	8.849534165588958	0.0
13	0.0	0.0	0.0	9.405104383858111	0.0
14	0.0	0.0	0.0	9.646599276834966	0.0
15	0.0	0.0	0.0	10.041172899786218	0.0
16	0.0	0.0	0.0	10.909498799123757	0.0
17	0.0	0.0	0.0	11.830610467417984	0.0
18	0.0	0.0	0.0	12.982659874897728	0.0
19	0.0	0.0	0.0	13.55802475652564	0.0
20	0.0	0.0	0.0	14.17825754176674	0.0
21	0.0	0.0	0.0	14.886906490010293	0.0
22	0.0	0.0	0.0	15.557285755760248	0.0
23	0.0	0.0	0.0	16.250098973316796	0.0
24	0.0	0.0	0.0	16.806988835809864	0.0
25	0.0	0.0	0.0	17.279421467972234	0.0
26	0.0	0.0	0.0	17.737338013671515	0.0
27	0.0	0.0	0.0	18.129272348174933	0.0
28	0.0	0.0	0.0	18.5937871149938	0.0
29	0.0	0.0	0.0	19.05566259336483	0.0
30	0.0	0.0	0.0	19.60331494629048	0.0
31	0.0	0.0	0.0	20.13777085697696	0.0
32	0.0	0.0	0.0	20.668267834991685	0.0
33	0.0	0.0	0.0	21.165773707408484	0.0
34	0.0	0.0	0.0	21.593338435957666	0.0
35	0.0	0.0	0.0	22.107999683285385	0.0
36	0.0	0.0	0.0	22.631898440180528	0.0
37	0.0	0.0	0.0	23.091134630103724	0.0
38	0.0	0.0	0.0	23.567526194937845	0.0
39	0.0	0.0	0.0	24.091424951832987	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCT	20	6.99159E-4	45.000004	5
ATAGGAT	35	1.1919474E-7	45.000004	5
TCCTGTA	20	6.99159E-4	45.000004	12
TTTAATG	20	6.99159E-4	45.000004	20
TAAACCA	20	6.99159E-4	45.000004	31
GTAGGGC	20	6.99159E-4	45.000004	4
CAAGGGT	20	6.99159E-4	45.000004	4
GGTATCC	20	6.99159E-4	45.000004	9
GTCAAGC	20	6.99159E-4	45.000004	16
GCGATGT	20	6.99159E-4	45.000004	9
CGAGACA	20	6.99159E-4	45.000004	22
CTAATGG	20	6.99159E-4	45.000004	42
AGTCGGG	20	6.99159E-4	45.000004	3
ATTGGGC	25	3.8562874E-5	44.999996	4
GGTTGCC	25	3.8562874E-5	44.999996	11
GCCAGAC	25	3.8562874E-5	44.999996	1
CAATCTT	25	3.8562874E-5	44.999996	17
GTGGTAT	25	3.8562874E-5	44.999996	25
TAGTAGG	25	3.8562874E-5	44.999996	2
CTATCTC	50	1.0531949E-9	40.499996	5
>>END_MODULE