##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936564.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	346120
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69461169536577	31.0	31.0	34.0	30.0	34.0
2	31.808055009823182	31.0	31.0	34.0	30.0	34.0
3	30.929307754535998	31.0	31.0	34.0	27.0	34.0
4	35.119787934820295	37.0	35.0	37.0	32.0	37.0
5	35.50681266612735	37.0	35.0	37.0	33.0	37.0
6	35.56181671096729	37.0	35.0	37.0	33.0	37.0
7	27.32541604067953	35.0	25.0	37.0	0.0	37.0
8	31.384248237605455	35.0	28.0	37.0	17.0	37.0
9	35.947038599329716	39.0	32.0	39.0	32.0	39.0
10	36.6153790592858	38.0	35.0	39.0	32.0	39.0
11	37.01110886397781	39.0	37.0	39.0	34.0	39.0
12	37.19989021148734	39.0	37.0	39.0	34.0	39.0
13	37.30159771177626	39.0	37.0	39.0	34.0	39.0
14	38.59943372240841	40.0	38.0	41.0	35.0	41.0
15	38.600381370622905	40.0	38.0	41.0	34.0	41.0
16	38.48428579683347	40.0	38.0	41.0	34.0	41.0
17	38.37228995724026	40.0	38.0	41.0	34.0	41.0
18	38.10702357563851	39.0	37.0	41.0	33.0	41.0
19	37.836082861435344	39.0	37.0	41.0	33.0	41.0
20	37.6975037559228	39.0	35.0	41.0	34.0	41.0
21	37.683540390615974	39.0	35.0	41.0	33.0	41.0
22	37.67042066335375	39.0	35.0	41.0	33.0	41.0
23	37.610805500982316	39.0	35.0	41.0	33.0	41.0
24	37.5388882468508	39.0	35.0	41.0	33.0	41.0
25	37.42018375130013	39.0	35.0	41.0	33.0	41.0
26	37.296440540852885	39.0	35.0	41.0	33.0	41.0
27	37.15797411302439	39.0	35.0	41.0	33.0	41.0
28	37.07074425054894	39.0	35.0	41.0	33.0	41.0
29	36.98022362186525	39.0	35.0	41.0	32.0	41.0
30	36.80365480180284	39.0	35.0	40.0	31.0	41.0
31	36.51383046342309	39.0	35.0	40.0	31.0	41.0
32	36.185756385068764	38.0	35.0	40.0	30.0	41.0
33	35.82311048191379	38.0	35.0	40.0	27.0	41.0
34	35.36667340806657	38.0	35.0	40.0	25.0	41.0
35	35.05245290650642	38.0	35.0	40.0	23.0	41.0
36	34.71445452444239	38.0	34.0	40.0	20.0	41.0
37	34.51378423668092	38.0	34.0	40.0	18.0	41.0
38	34.298899225702066	38.0	34.0	40.0	18.0	41.0
39	34.088310412573676	38.0	33.0	40.0	15.0	41.0
40	33.84001502369121	38.0	33.0	40.0	15.0	41.0
41	33.65043626487923	38.0	33.0	40.0	15.0	41.0
42	33.447847567317694	38.0	33.0	40.0	14.0	41.0
43	33.33451115220155	38.0	33.0	40.0	12.0	41.0
44	33.07426903963943	38.0	32.0	40.0	10.0	41.0
45	32.978183866866985	38.0	31.0	40.0	10.0	41.0
46	32.760481913787125	38.0	31.0	40.0	10.0	41.0
47	32.52402346007165	37.0	31.0	40.0	10.0	41.0
48	32.34774644631919	37.0	30.0	40.0	10.0	41.0
49	32.14492083670403	37.0	30.0	40.0	9.0	41.0
50	31.905165838437537	37.0	29.0	40.0	8.0	41.0
51	29.919623252051313	35.0	24.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	15.0
10	16.0
11	19.0
12	10.0
13	8.0
14	7.0
15	14.0
16	28.0
17	55.0
18	134.0
19	269.0
20	525.0
21	873.0
22	1529.0
23	2696.0
24	4483.0
25	7370.0
26	9520.0
27	9986.0
28	9597.0
29	9074.0
30	9459.0
31	10137.0
32	11853.0
33	14683.0
34	20209.0
35	25754.0
36	29888.0
37	46580.0
38	58994.0
39	62319.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.73962787472553	18.187333872645326	20.859239570091297	25.213798682537846
2	39.870565121923036	23.03247428637467	22.362764359181785	14.734196232520514
3	26.796486767595052	22.89379405986363	35.86732924997111	14.442389922570205
4	22.91546284525598	22.663816017566162	38.23298278053854	16.18773835663932
5	22.20039292730845	26.23714318733387	34.50450710736161	17.05795677799607
6	21.671963480873686	30.813012827920954	34.351092106783774	13.163931584421587
7	52.96949035016757	25.47555761007743	17.256442852189995	4.298509187565006
8	71.8288454871143	4.143360684155784	19.583092569051196	4.444701259678724
9	65.71824800647175	5.882064024037906	21.490523517855078	6.90916445163527
10	31.54108401710389	23.03507454062175	29.924592626834624	15.499248815439731
11	22.43730498093147	23.549924881543973	35.815613082168035	18.197157055356524
12	19.439789668323122	21.149890211487346	39.629319311221536	19.78100080896799
13	18.71460765052583	21.71067837744135	42.218883624176584	17.355830347856234
14	17.805963249739975	25.184906968681382	39.111868716052236	17.897261065526408
15	17.346584999422166	25.653241650294696	41.079105512539	15.921067837744134
16	19.358603952386456	25.61597133941985	37.65023691205363	17.375187796140068
17	19.412631457298048	26.182248931006587	35.92886860048538	18.476251011209985
18	19.76857737200971	25.52380677221773	36.28539234947417	18.422223506298394
19	18.801860626372356	28.152085981740438	33.97983358372819	19.066219808159023
20	19.018837397434414	26.60840171038946	35.60845949381717	18.764301398358953
21	19.30255402750491	27.311626025655844	36.398070033514394	16.98774991332486
22	18.854443545591124	23.941407604299087	36.200739627874725	21.003409222235064
23	17.90910666820756	25.483358372818678	36.572287068068874	20.035247890904888
24	19.188720674910435	24.51635271004276	36.69969952617589	19.59522708887091
25	19.025193574482838	27.294579914480526	34.48486074193921	19.19536576909742
26	18.657402057090028	27.125274471281635	34.526175892753955	19.69114757887438
27	19.579914480526988	26.744192765514853	33.54096845024847	20.134924303709695
28	19.107534958973766	26.3628221426095	34.81509303131862	19.714549867098118
29	18.577372009707616	27.075002889171383	33.94343002426904	20.40419507685196
30	18.131572864902346	28.52103316768751	33.96769906390847	19.379694903501676
31	17.837166300704958	28.982144920836706	33.19831272391078	19.982376054547558
32	17.72679995377326	28.500520050849413	34.485438576216346	19.287241419160985
33	17.208193690049693	27.09811626025656	33.66202473130706	22.031665318386686
34	16.523171154512887	27.479197966023346	32.74009014214723	23.257540737316535
35	16.560441465387726	26.75979429099734	32.33271697677107	24.34704726684387
36	17.208193690049693	27.285912400323586	30.71420316653184	24.79169074309488
37	17.120073962787473	26.959147116606957	32.88512654570669	23.03565237489888
38	17.310759274240148	26.948457182480062	31.423783658846645	24.316999884433145
39	18.778747255287183	26.03981278169421	31.312261643360685	23.869178319657923
40	19.05870796255634	25.56945568011094	32.4468392465041	22.924997110828617
41	17.880792788628224	26.967236796486766	31.612735467467928	23.539234947417082
42	18.743499364382295	27.337050733849534	32.275222466196695	21.644227435571477
43	19.69230324742864	26.9629030394083	31.853981278169417	21.490812434993643
44	19.891944990176817	26.84040217265688	30.97018375130013	22.297469085866172
45	18.576505258291924	26.062348318502256	31.34606494857275	24.015081474633078
46	20.271871027389345	26.718479140182595	29.429677568473362	23.579972263954698
47	19.144805269848607	25.99705304518664	32.361030856350396	22.497110828614353
48	18.91078238761123	25.557032243152666	31.53097191725413	24.00121345198197
49	18.51323240494626	25.331099040795102	32.73922339073154	23.4164451635271
50	17.89754998266497	24.667167456373512	34.27539581647983	23.159886744481682
51	17.821564775222466	24.343291344042527	32.998670981162604	24.836472899572403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	206.5
2	303.0
3	7066.5
4	13830.0
5	9715.0
6	5600.0
7	4874.5
8	4149.0
9	3945.5
10	3742.0
11	3642.5
12	3543.0
13	3394.0
14	3245.0
15	3073.5
16	2902.0
17	2740.5
18	2579.0
19	2427.0
20	2275.0
21	2259.5
22	2244.0
23	2294.0
24	2344.0
25	2536.5
26	3166.5
27	3604.0
28	4050.5
29	4497.0
30	5227.0
31	5957.0
32	6922.5
33	7888.0
34	9101.0
35	10314.0
36	11425.0
37	12536.0
38	13547.5
39	14559.0
40	15760.0
41	16961.0
42	18265.0
43	19569.0
44	21983.0
45	24397.0
46	30497.5
47	36598.0
48	35093.0
49	33588.0
50	31163.0
51	28738.0
52	24761.0
53	20784.0
54	17925.0
55	15066.0
56	13314.5
57	11563.0
58	10534.0
59	9505.0
60	8267.5
61	7030.0
62	5987.0
63	4944.0
64	4081.0
65	3218.0
66	2613.5
67	2009.0
68	1617.5
69	1226.0
70	991.0
71	756.0
72	610.5
73	465.0
74	402.0
75	267.0
76	195.0
77	153.0
78	111.0
79	80.0
80	49.0
81	37.5
82	26.0
83	16.0
84	6.0
85	10.0
86	14.0
87	9.5
88	5.0
89	6.0
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	346120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.64306370109257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00256558536448	51.71610943385553
2	10.55610944557324	13.647585106517921
3	4.127186802877222	8.003819982141026
4	2.2040702840479818	5.6991142309359555
5	1.2447851801665035	4.023336384783964
6	0.6917182619489202	2.682887260222385
7	0.41235710583164364	1.8659218661911177
8	0.2408669265937716	1.2456300863430054
9	0.14116541235730673	0.8212828269063941
>10	0.3521529169708325	3.572607911916231
>50	0.011517607130698292	0.5088599508138894
>100	0.010631637351347393	1.4684489745419713
>500	0.0026579093378368483	1.114509245108688
>1k	0.0017719395585578987	1.5489158547772082
>5k	4.4298488963947466E-4	2.0809708849447164
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7267	2.0995608459493815	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	1629	0.4706460187218306	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTT	1490	0.4304865364613429	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1230	0.3553680804345314	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTC	1060	0.30625216687853923	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCT	825	0.23835663931584422	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	738	0.21322084826071883	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCG	641	0.18519588581994684	No Hit
CGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	593	0.1713278631688432	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGC	568	0.1641049347047267	No Hit
GAACTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	527	0.15225933202357564	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCT	450	0.13001271235409684	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14561423783658847	0.0
2	0.0	0.0	0.0	0.6012365653530567	0.0
3	0.0	0.0	0.0	1.0759274240147925	0.0
4	0.0	0.0	0.0	1.7283023228937942	0.0
5	0.0	0.0	0.0	3.5224777533803304	0.0
6	0.0	0.0	0.0	5.348145151970415	0.0
7	0.0	0.0	0.0	6.53646134288686	0.0
8	0.0	0.0	0.0	8.206691320929158	0.0
9	0.0	0.0	0.0	9.155206286836934	0.0
10	0.0	0.0	0.0	10.553276320351323	0.0
11	0.0	0.0	0.0	11.399803536345775	0.0
12	0.0	0.0	0.0	12.377499133248584	0.0
13	0.0	0.0	0.0	12.946665896220964	0.0
14	0.0	0.0	0.0	13.28123194267884	0.0
15	0.0	0.0	0.0	13.571593666936323	0.0
16	0.0	0.0	0.0	13.95036403559459	0.0
17	0.0	0.0	0.0	14.35889286952502	0.0
18	0.0	0.0	0.0	14.865942447705997	0.0
19	0.0	0.0	0.0	15.190107477175546	0.0
20	0.0	0.0	0.0	15.540275049115914	0.0
21	0.0	0.0	0.0	15.882064024037906	0.0
22	0.0	0.0	0.0	16.247255287183634	0.0
23	0.0	0.0	0.0	16.64740552409569	0.0
24	0.0	0.0	0.0	17.007396278747255	0.0
25	0.0	0.0	0.0	17.35178550791633	0.0
26	0.0	0.0	0.0	17.6537039177164	0.0
27	0.0	0.0	0.0	17.99664856119265	0.0
28	0.0	0.0	0.0	18.33872645325321	0.0
29	0.0	0.0	0.0	18.726742170345545	0.0
30	0.0	0.0	0.0	19.170807812319428	0.0
31	0.0	0.0	0.0	19.551600600947648	0.0
32	0.0	0.0	0.0	19.930370969605917	0.0
33	0.0	0.0	0.0	20.28458338148619	0.0
34	0.0	0.0	0.0	20.65555298740321	0.0
35	0.0	0.0	0.0	21.001097885126544	0.0
36	0.0	0.0	0.0	21.352132208482608	0.0
37	0.0	0.0	0.0	21.702877614700103	0.0
38	0.0	0.0	0.0	22.075002889171387	0.0
39	0.0	0.0	0.0	22.430082052467352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.026448E-4	45.0	25
CTCACGA	20	7.026448E-4	45.0	24
CACGGGC	20	7.026448E-4	45.0	4
CGTTCTG	180	0.0	45.0	1
CGTTAAG	20	7.026448E-4	45.0	2
GCGATCG	20	7.026448E-4	45.0	9
CGGTCTA	20	7.026448E-4	45.0	31
TATCCGG	20	7.026448E-4	45.0	2
CGTTTCA	30	2.1610904E-6	44.999996	1
CGTTTTA	645	0.0	43.95349	1
CGTTTTT	4030	0.0	43.380898	1
AGGCGAT	120	0.0	43.124996	7
CGTTCTA	70	0.0	41.785713	1
CGGTTTT	315	0.0	41.428574	1
CTGGGTC	60	3.6379788E-12	41.249996	5
CGTTTCT	230	0.0	41.08696	1
GTTTTAT	700	0.0	40.17857	2
ATTGGGC	45	1.9215804E-8	40.0	4
CGGTTTA	75	0.0	39.000004	1
CGCTGTA	35	6.235643E-6	38.571426	1
>>END_MODULE