##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936563.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153885
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.058972609416124	33.0	31.0	34.0	30.0	34.0
2	32.181726614029955	33.0	31.0	34.0	30.0	34.0
3	32.00651785424181	33.0	31.0	34.0	30.0	34.0
4	35.77190109497352	37.0	35.0	37.0	35.0	37.0
5	35.867024076420705	37.0	35.0	37.0	35.0	37.0
6	35.838021899470384	37.0	35.0	37.0	35.0	37.0
7	26.80048087857816	36.0	10.0	37.0	0.0	37.0
8	31.165474217760014	36.0	19.0	37.0	17.0	37.0
9	36.013698541118366	39.0	32.0	39.0	32.0	39.0
10	36.75328329596777	38.0	35.0	39.0	33.0	39.0
11	37.111167430223865	39.0	37.0	39.0	34.0	39.0
12	37.10495499886279	39.0	37.0	39.0	34.0	39.0
13	37.02723462325763	39.0	37.0	39.0	33.0	39.0
14	38.10206322903467	40.0	37.0	41.0	33.0	41.0
15	38.221931962179546	40.0	37.0	41.0	33.0	41.0
16	38.21753907138447	40.0	37.0	41.0	33.0	41.0
17	38.12695194463398	40.0	37.0	41.0	33.0	41.0
18	38.02311466354745	40.0	37.0	41.0	33.0	41.0
19	37.90244663222536	40.0	37.0	41.0	33.0	41.0
20	37.928004678818596	40.0	36.0	41.0	33.0	41.0
21	37.84567046820678	40.0	36.0	41.0	33.0	41.0
22	37.88154141079377	40.0	36.0	41.0	33.0	41.0
23	37.80872729635767	40.0	36.0	41.0	33.0	41.0
24	37.748318549566235	40.0	36.0	41.0	33.0	41.0
25	37.63224485817331	40.0	36.0	41.0	33.0	41.0
26	37.520174156025604	40.0	35.0	41.0	33.0	41.0
27	37.4136205608084	39.0	35.0	41.0	33.0	41.0
28	37.35121681775352	39.0	35.0	41.0	33.0	41.0
29	37.292458654189815	39.0	35.0	41.0	32.0	41.0
30	37.179887578386456	39.0	35.0	41.0	32.0	41.0
31	37.0520388601878	39.0	35.0	41.0	31.0	41.0
32	36.888618123923706	39.0	35.0	41.0	31.0	41.0
33	36.76668941092374	39.0	35.0	41.0	31.0	41.0
34	36.55186665366995	39.0	35.0	41.0	30.0	41.0
35	36.44541703219937	39.0	35.0	41.0	30.0	41.0
36	36.22369301751308	39.0	35.0	41.0	30.0	41.0
37	36.11686649121097	39.0	35.0	41.0	29.0	41.0
38	35.90647561490724	39.0	35.0	40.0	28.0	41.0
39	35.80521818240894	39.0	35.0	40.0	28.0	41.0
40	35.62440783702115	38.0	35.0	40.0	27.0	41.0
41	35.50180979302726	38.0	35.0	40.0	26.0	41.0
42	35.39765409234168	38.0	35.0	40.0	26.0	41.0
43	35.38543717711278	38.0	34.0	40.0	26.0	41.0
44	35.19778405952497	38.0	34.0	40.0	26.0	41.0
45	35.08135295837801	38.0	34.0	40.0	25.0	41.0
46	34.99289729343341	38.0	34.0	40.0	25.0	41.0
47	34.83674172271501	38.0	34.0	40.0	24.0	41.0
48	34.75624654774669	37.0	34.0	40.0	24.0	41.0
49	34.62552555479741	37.0	34.0	40.0	24.0	41.0
50	34.41905318906976	37.0	33.0	40.0	23.0	41.0
51	32.355674692140234	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	7.0
10	12.0
11	12.0
12	6.0
13	6.0
14	10.0
15	17.0
16	18.0
17	53.0
18	86.0
19	148.0
20	242.0
21	351.0
22	534.0
23	784.0
24	1082.0
25	1490.0
26	1896.0
27	2091.0
28	2409.0
29	2582.0
30	3040.0
31	3957.0
32	4995.0
33	7097.0
34	10834.0
35	13254.0
36	14721.0
37	23214.0
38	28644.0
39	30280.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.70266757643695	19.790752834909185	24.63333008415375	12.873249504500114
2	32.03951002371901	24.29671507944244	26.1721415342626	17.49163336257595
3	32.14803262176301	23.80024043928908	27.34184618383858	16.709880755109335
4	27.900055236052896	25.57299281931312	27.598531370828866	18.928420573805113
5	25.691262956103582	30.261558956363515	24.99203951002372	19.05513857750918
6	27.332098645092113	34.76232251356532	24.10176430451311	13.803814536829451
7	62.38944666471716	27.816876238749717	6.278714624557299	3.5149624719758266
8	85.31240861682426	4.665821879975306	6.969490203723559	3.052279299476882
9	79.302076225753	7.155993111739285	8.789030769730644	4.752899892777074
10	41.94235955421256	30.6852519738766	14.506287162491471	12.866101309419372
11	33.14942976898333	26.349546739448286	23.390193976021052	17.110829515547323
12	29.30565032329337	24.44812684797089	26.99938265587939	19.246840172856352
13	26.0259284530656	25.84267472463203	29.054163823634532	19.07723299866784
14	22.403743054878646	27.298307177437696	30.177080287227476	20.120869480456186
15	21.075478441693473	25.125255872892094	33.29564285018033	20.503622835234104
16	26.720603047730446	25.419631543035383	28.662962601943008	19.196802807291157
17	27.046170841862434	24.564447477012056	26.75634402313416	21.63303765799136
18	27.77723624784742	23.401891022516814	28.211326640023394	20.609546089612373
19	27.01042986645872	26.193586119504825	26.154595964518958	20.6413880495175
20	28.313350878903076	26.295610358384508	26.319004451376028	19.072034311336388
21	26.713454852649704	26.116905481365954	27.90265457971862	19.266985086265716
22	26.773239756961363	23.29466809630568	27.45166845371544	22.480423693017514
23	24.045228579783604	26.528251616466843	27.835071644409787	21.591448159339766
24	23.77684634629756	25.79068785131754	29.207525099912274	21.224940702472626
25	24.2791695096988	26.78688631120642	27.054618708776033	21.879325470318744
26	24.083568898853038	26.870715144426033	26.775839100627092	22.269876856093838
27	23.65662670175781	26.400883776846346	27.82467426974689	22.11781525164896
28	21.53426259869383	27.449069110049713	28.32764726906456	22.689021022191895
29	23.081521915716284	27.00523117912727	27.66026578288982	22.252981122266625
30	25.300711570328495	25.88946291061507	27.84936803457127	20.96045748448517
31	26.019430093901292	26.27351593722585	26.429476557169313	21.277577411703543
32	26.72320239139617	26.005783539656235	25.78938817948468	21.48162588946291
33	26.133801215193163	25.95444650225818	25.242226337849694	22.66952594469896
34	23.11206420378854	28.0254735679241	27.78243493517887	21.08002729310849
35	24.11281151509244	28.069662410241413	26.87851317542321	20.939012899242943
36	25.899860285277963	28.86051272053806	25.120057185560647	20.11956980862332
37	24.505962244533254	28.75199012249407	25.242876173766128	21.49917145920655
38	24.20638788705852	28.86376190012022	25.44627481560906	21.483575397212203
39	24.28501803294668	27.04032231861455	25.550898398154466	23.123761250284304
40	26.631575527179386	25.899210449361536	26.23777496182214	21.231439061636937
41	23.372648406277417	25.89661110569581	26.578938817948465	24.151801670078303
42	24.451376027553042	26.386587386684862	26.647171589173734	22.51486499658836
43	24.115410858758164	26.15654547226825	26.88176235500536	22.846281313968223
44	23.791142736459044	26.28326347597232	27.131299346914904	22.794294440653733
45	23.45647723949703	26.228677258992107	26.354745426779736	23.96010007473113
46	24.010787276212756	26.92270201774052	25.549598726321605	23.51691197972512
47	22.937258342268578	27.213828508301653	27.368489456412252	22.480423693017514
48	23.273223511063456	27.144945901159957	27.744094616109432	21.837735971667154
49	24.67426974688891	25.899860285277963	27.066965591188225	22.358904376644897
50	22.50121844234331	27.50625467069565	27.44192091496897	22.55060597199207
51	23.46947395782565	26.460018845241578	25.613932482048284	24.45657471488449
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	135.5
2	226.0
3	730.5
4	1235.0
5	870.0
6	505.0
7	444.5
8	384.0
9	384.0
10	384.0
11	377.0
12	370.0
13	369.0
14	368.0
15	375.0
16	382.0
17	382.0
18	382.0
19	373.5
20	365.0
21	450.5
22	536.0
23	599.0
24	662.0
25	792.5
26	1035.5
27	1148.0
28	1355.0
29	1562.0
30	1991.5
31	2421.0
32	2843.5
33	3266.0
34	3763.5
35	4261.0
36	4645.5
37	5030.0
38	5387.0
39	5744.0
40	6533.0
41	7322.0
42	7900.5
43	8479.0
44	9214.5
45	9950.0
46	10865.0
47	11780.0
48	11896.0
49	12012.0
50	12075.5
51	12139.0
52	11564.0
53	10989.0
54	10199.5
55	9410.0
56	8973.0
57	8536.0
58	8026.5
59	7517.0
60	6764.5
61	6012.0
62	5638.0
63	5264.0
64	4722.5
65	4181.0
66	3607.0
67	3033.0
68	2720.0
69	2407.0
70	2030.5
71	1654.0
72	1352.0
73	1050.0
74	943.5
75	695.0
76	553.0
77	420.5
78	288.0
79	202.5
80	117.0
81	91.0
82	65.0
83	47.5
84	30.0
85	24.5
86	19.0
87	20.5
88	22.0
89	16.0
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	153885.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.368879357962115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.4676592999279	35.171069305000486
2	12.006476995281409	12.335185365695162
3	6.422598641349037	9.897650843162102
4	4.092398385811332	8.40887675861845
5	2.7552530708800873	7.076713129934692
6	1.9456286607041202	5.9966858368262015
7	1.3991321838353425	5.031029665009585
8	0.9766094447747599	4.013386619878481
9	0.6401092992953737	2.9593527634272347
>10	1.2802185985907475	8.146992884296715
>50	0.010120305127199584	0.3769048315300387
>100	0.002530076281799896	0.1787048770185528
>500	0.001265038140899948	0.4074471196023004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	627	0.4074471196023004	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	166	0.1078727621275628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.044188842317314875	0.0
2	0.0	0.0	0.0	0.1754556974363973	0.0
3	0.0	0.0	0.0	0.24238879682880074	0.0
4	0.0	0.0	0.0	0.353510738538519	0.0
5	0.0	0.0	0.0	0.6855768918348117	0.0
6	0.0	0.0	0.0	0.9266660168307502	0.0
7	0.0	0.0	0.0	1.1580076030802222	0.0
8	0.0	0.0	0.0	1.6668291256457743	0.0
9	0.0	0.0	0.0	1.9241641485524905	0.0
10	0.0	0.0	0.0	2.4388341943659224	0.0
11	0.0	0.0	0.0	2.8924196640348314	0.0
12	0.0	0.0	0.0	3.346654969620171	0.0
13	0.0	0.0	0.0	3.5500536114631056	0.0
14	0.0	0.0	0.0	3.6566267017578062	0.0
15	0.0	0.0	0.0	3.7891932287097507	0.0
16	0.0	0.0	0.0	4.025083666374241	0.0
17	0.0	0.0	0.0	4.330506547096858	0.0
18	0.0	0.0	0.0	4.643727458816649	0.0
19	0.0	0.0	0.0	4.865321506319654	0.0
20	0.0	0.0	0.0	5.08691555382266	0.0
21	0.0	0.0	0.0	5.338402053481496	0.0
22	0.0	0.0	0.0	5.58858888130747	0.0
23	0.0	0.0	0.0	5.858920622542808	0.0
24	0.0	0.0	0.0	6.073366474965072	0.0
25	0.0	0.0	0.0	6.27351593722585	0.0
26	0.0	0.0	0.0	6.45027130649511	0.0
27	0.0	0.0	0.0	6.68031322091172	0.0
28	0.0	0.0	0.0	6.906456119829743	0.0
29	0.0	0.0	0.0	7.154693439906423	0.0
30	0.0	0.0	0.0	7.504305162946356	0.0
31	0.0	0.0	0.0	7.781135263346005	0.0
32	0.0	0.0	0.0	8.024173896091236	0.0
33	0.0	0.0	0.0	8.2386197485135	0.0
34	0.0	0.0	0.0	8.518049192578873	0.0
35	0.0	0.0	0.0	8.826721252883647	0.0
36	0.0	0.0	0.0	9.080807096208208	0.0
37	0.0	0.0	0.0	9.354388017025702	0.0
38	0.0	0.0	0.0	9.687753842154855	0.0
39	0.0	0.0	0.0	10.216070442213342	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	60	0.0	45.000004	21
GTTGATC	25	3.875017E-5	45.000004	16
ACACGTG	60	0.0	45.000004	42
ACTAAGG	25	3.875017E-5	45.000004	3
CGCATAG	20	7.0142304E-4	45.0	2
AGGCGAT	70	0.0	45.0	7
TTATCGT	20	7.0142304E-4	45.0	23
TACGCGG	20	7.0142304E-4	45.0	2
AGTAGCG	20	7.0142304E-4	45.0	1
TATCGTA	20	7.0142304E-4	45.0	24
GTTATCG	20	7.0142304E-4	45.0	22
CGACCAA	65	0.0	41.53846	29
CACGACC	65	0.0	41.53846	27
TCAAGCG	65	0.0	41.53846	17
ACGACCA	65	0.0	41.53846	28
AGCGACT	55	6.002665E-11	40.909092	19
GCCCTAG	50	1.0677468E-9	40.500004	20
CGAATAT	50	1.0677468E-9	40.500004	14
ATGGGCG	35	6.2130803E-6	38.571426	5
ACACGAC	70	0.0	38.571426	26
>>END_MODULE