##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936562.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200698
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900023916531307	31.0	31.0	34.0	30.0	34.0
2	32.00498759329938	33.0	31.0	34.0	30.0	34.0
3	31.870511913422156	33.0	31.0	34.0	30.0	34.0
4	35.65798861971719	37.0	35.0	37.0	33.0	37.0
5	35.76063538251502	37.0	35.0	37.0	35.0	37.0
6	35.738691965042	37.0	35.0	37.0	35.0	37.0
7	26.936257461459505	35.0	11.0	37.0	0.0	37.0
8	31.21366929416337	35.0	19.0	37.0	17.0	37.0
9	35.96566482974419	39.0	32.0	39.0	32.0	39.0
10	36.58095247585925	38.0	35.0	39.0	32.0	39.0
11	36.92676060548685	39.0	37.0	39.0	33.0	39.0
12	37.03894408514285	39.0	37.0	39.0	34.0	39.0
13	37.02730968918475	39.0	37.0	39.0	33.0	39.0
14	38.225602646762795	40.0	37.0	41.0	33.0	41.0
15	38.29270844751816	40.0	37.0	41.0	34.0	41.0
16	38.227112377801475	40.0	37.0	41.0	34.0	41.0
17	38.138068142183776	40.0	37.0	41.0	33.0	41.0
18	38.02125581719798	40.0	37.0	41.0	33.0	41.0
19	37.895275488544975	40.0	37.0	41.0	33.0	41.0
20	37.96793191760755	40.0	37.0	41.0	33.0	41.0
21	37.93103070284706	40.0	37.0	41.0	33.0	41.0
22	37.924533378509004	40.0	37.0	41.0	33.0	41.0
23	37.89362624440702	40.0	36.0	41.0	33.0	41.0
24	37.842315319534826	40.0	36.0	41.0	33.0	41.0
25	37.73941444359186	40.0	36.0	41.0	33.0	41.0
26	37.62848159921872	40.0	36.0	41.0	33.0	41.0
27	37.545536079083995	39.0	36.0	41.0	33.0	41.0
28	37.51394632731766	40.0	36.0	41.0	33.0	41.0
29	37.48739897756829	40.0	36.0	41.0	33.0	41.0
30	37.43719917487967	40.0	36.0	41.0	33.0	41.0
31	37.33325195069209	39.0	36.0	41.0	32.0	41.0
32	37.111735044694015	39.0	35.0	41.0	31.0	41.0
33	37.02770331542915	39.0	35.0	41.0	31.0	41.0
34	36.93818573179603	39.0	35.0	41.0	31.0	41.0
35	36.809450019432184	39.0	35.0	41.0	31.0	41.0
36	36.67392799131033	39.0	35.0	41.0	30.0	41.0
37	36.529457194391576	39.0	35.0	41.0	30.0	41.0
38	36.39297850501749	39.0	35.0	41.0	30.0	41.0
39	36.36827472122293	39.0	35.0	40.0	30.0	41.0
40	36.260007573568245	39.0	35.0	40.0	30.0	41.0
41	36.21813371334044	39.0	35.0	40.0	30.0	41.0
42	36.1576298717476	39.0	35.0	40.0	29.0	41.0
43	36.12617963308055	39.0	35.0	40.0	29.0	41.0
44	35.91450836580334	39.0	35.0	40.0	28.0	41.0
45	35.77673918026089	38.0	35.0	40.0	27.0	41.0
46	35.67743574923517	38.0	35.0	40.0	27.0	41.0
47	35.58261666782928	38.0	34.0	40.0	27.0	41.0
48	35.49081206589005	38.0	34.0	40.0	26.0	41.0
49	35.27349051809186	38.0	34.0	40.0	26.0	41.0
50	35.05940766724133	38.0	34.0	40.0	26.0	41.0
51	32.802359764422164	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	8.0
11	11.0
12	2.0
13	3.0
14	9.0
15	11.0
16	11.0
17	29.0
18	75.0
19	146.0
20	187.0
21	353.0
22	479.0
23	723.0
24	1098.0
25	1520.0
26	1983.0
27	2268.0
28	2765.0
29	3128.0
30	4120.0
31	5041.0
32	6682.0
33	9092.0
34	13390.0
35	17163.0
36	19526.0
37	30191.0
38	38736.0
39	41927.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92667590110514	20.62950303440991	25.067016113762968	12.37680495072198
2	30.089985949037857	26.079980866774953	27.077997787720854	16.752035396466333
3	30.933043677565298	25.097908300032884	28.297740884313743	15.671307138088073
4	27.412829226001257	24.75709772892605	29.19710211362345	18.63297093144924
5	25.155706584021765	30.60020528356037	25.889146877397884	18.35494125501998
6	24.75610120678831	36.212617963308055	25.488544978026688	13.54273585187695
7	61.95178825897617	27.174162174012693	7.008540194720425	3.8655093722907057
8	84.30577285274393	5.049875932993851	7.107694147425485	3.5366570668367396
9	78.26136782628626	6.877995794676579	8.770391334243492	6.09024504479367
10	38.62270675343053	30.219533826943966	16.78741193235608	14.37034748726943
11	28.044126000259094	26.838334213594557	26.346550538620217	18.770989247526135
12	26.740176783027238	22.698282992356674	31.167226379934032	19.39431384468206
13	23.609104226250384	26.04360780874747	31.43927692353686	18.908011041465286
14	19.70074440203689	30.07752942231612	29.450716997678107	20.77100917796889
15	18.755543154391173	26.95941165332988	34.87727829873741	19.407766893541538
16	21.240869365912964	25.894627749155447	32.03569542297382	20.82880746195777
17	22.335050673150704	24.806425574744143	29.44075177630071	23.417771975804442
18	22.362953293007404	26.415808827193093	30.63558181945012	20.58565606034938
19	23.95788697445914	28.210545197261556	27.420303142034303	20.411264686245005
20	23.740645148431973	27.820406780336626	29.018226389899255	19.42072168133215
21	23.088421409281608	27.625586702408594	29.157241228113882	20.128750660195916
22	21.572212976711278	25.811916411723086	28.76760107225782	23.848269539307815
23	20.788448315379327	27.699329340601302	29.09047424488535	22.421748099134025
24	20.917497932216563	25.91206688656589	30.344597355230245	22.825837825987307
25	20.550279524459636	28.93950114101785	28.7481688905719	21.762050443950613
26	19.97130016243311	28.946476795982022	28.72325583712842	22.358967204456448
27	21.24635023767053	28.37347656678193	28.58374273784492	21.796430457702616
28	19.402286021783972	27.458669244337266	31.0511315508874	22.087913182991358
29	20.703245672602616	25.563782399426003	30.08948768796899	23.64348424000239
30	22.05801751885918	26.986317751048837	29.611655322922996	21.34400940716898
31	23.425744152906358	26.87769683803526	27.24989785648088	22.446661152577505
32	23.09539706424578	27.24391872365445	28.38493657136593	21.27574764073384
33	22.04605925320631	26.757615920437672	29.460682219055496	21.73564260730052
34	20.7839639657595	26.15322524389879	30.5653270087395	22.49748378160221
35	22.079442744820575	24.825857756430057	29.947981544410005	23.146717954339355
36	23.32310237271921	26.35551923785987	29.492072666394286	20.829305723026636
37	22.247356725029647	26.692841981484616	31.69986746255568	19.359933830930053
38	21.619547778253896	27.21003697097131	30.11639378568795	21.054021465086848
39	21.199513697196785	25.191581380980377	32.48114081854329	21.127764103279553
40	22.371921992247056	24.903586483173722	31.39393516626972	21.3305563583095
41	20.36243510149578	25.86373556288553	30.50154959192418	23.272279743694508
42	21.676349540105033	25.705786804053854	30.050623324597154	22.56724033124396
43	22.483034210604988	25.399854507767888	30.046138974977328	22.070972306649793
44	21.65193474773042	25.616598072726187	29.857298029875736	22.874169149667658
45	21.060000597913284	25.68236853381698	29.113892515122224	24.143738353147516
46	22.388364607519755	26.558311492889814	29.064564669304126	21.988759230286302
47	20.822828329131333	25.257351842071174	32.15477981843367	21.76504001036383
48	21.733649563025043	23.82734257441529	30.89019322564251	23.548814636917157
49	20.897567489461778	23.859729543891817	32.11691197719958	23.12579098944683
50	20.14917936401957	24.47856979142792	31.504050862489912	23.868199982062603
51	20.663384787093044	24.44568456088252	29.28728736708886	25.603643284935572
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	186.0
2	314.0
3	800.5
4	1287.0
5	896.0
6	505.0
7	484.5
8	464.0
9	439.0
10	414.0
11	412.0
12	410.0
13	398.5
14	387.0
15	404.5
16	422.0
17	435.0
18	448.0
19	540.0
20	632.0
21	747.5
22	863.0
23	979.0
24	1095.0
25	1363.5
26	1949.0
27	2266.0
28	2773.5
29	3281.0
30	3822.5
31	4364.0
32	4894.5
33	5425.0
34	6219.0
35	7013.0
36	7512.5
37	8012.0
38	8672.5
39	9333.0
40	10075.5
41	10818.0
42	11846.0
43	12874.0
44	14046.0
45	15218.0
46	17921.0
47	20624.0
48	19828.5
49	19033.0
50	18218.5
51	17404.0
52	15193.5
53	12983.0
54	11415.0
55	9847.0
56	9017.0
57	8187.0
58	7583.5
59	6980.0
60	6447.0
61	5914.0
62	5035.5
63	4157.0
64	3565.5
65	2974.0
66	2329.0
67	1684.0
68	1470.5
69	1257.0
70	1039.0
71	821.0
72	673.0
73	525.0
74	441.5
75	281.5
76	205.0
77	159.0
78	113.0
79	76.5
80	40.0
81	33.5
82	27.0
83	21.5
84	16.0
85	10.5
86	5.0
87	5.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200698.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.2224851731377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.31499937988974	36.318389841519746
2	10.031999913974333	10.076639338729892
3	5.081999956421209	7.656920023837517
4	3.268000815352582	6.565084899793878
5	2.392999993391479	6.009120334371108
6	1.6859999857630883	5.080506557213825
7	1.336999988538998	4.700322347061957
8	1.0579999909275868	4.2508311086032435
9	0.7589999934914804	3.430697932758373
>10	2.0389999825153176	13.088983973583456
>50	0.013999999879948233	0.44748233905543927
>100	0.012999999888523359	1.0606988777610413
>500	0.003999999965699495	1.3143224257105326
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	744	0.37070623523901586	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	723	0.3602427527927533	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.2984583802529173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	551	0.2745418489471743	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	325	0.1619348473826346	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12805309469949874	0.0
2	0.0	0.0	0.0	0.6043906765388793	0.0
3	0.0	0.0	0.0	0.8056881483622159	0.0
4	0.0	0.0	0.0	1.1006587011330458	0.0
5	0.0	0.0	0.0	2.2008191411972216	0.0
6	0.0	0.0	0.0	2.7220002192348702	0.0
7	0.0	0.0	0.0	3.2476656468923455	0.0
8	0.0	0.0	0.0	4.184396456367279	0.0
9	0.0	0.0	0.0	4.559587041226121	0.0
10	0.0	0.0	0.0	5.971658910402694	0.0
11	0.0	0.0	0.0	7.162502865001146	0.0
12	0.0	0.0	0.0	8.371782479147774	0.0
13	0.0	0.0	0.0	8.764910462485924	0.0
14	0.0	0.0	0.0	8.918873132766645	0.0
15	0.0	0.0	0.0	9.241248044325305	0.0
16	0.0	0.0	0.0	9.872544818583146	0.0
17	0.0	0.0	0.0	10.667769484499098	0.0
18	0.0	0.0	0.0	11.530757655781324	0.0
19	0.0	0.0	0.0	12.034997857477403	0.0
20	0.0	0.0	0.0	12.507349350765827	0.0
21	0.0	0.0	0.0	13.118715682268881	0.0
22	0.0	0.0	0.0	13.763465505386202	0.0
23	0.0	0.0	0.0	14.434124904084744	0.0
24	0.0	0.0	0.0	14.932884234023259	0.0
25	0.0	0.0	0.0	15.383810501350288	0.0
26	0.0	0.0	0.0	15.794377622098875	0.0
27	0.0	0.0	0.0	16.23533866804851	0.0
28	0.0	0.0	0.0	16.667829275827362	0.0
29	0.0	0.0	0.0	17.137191202702567	0.0
30	0.0	0.0	0.0	17.663354891428913	0.0
31	0.0	0.0	0.0	18.153145522127772	0.0
32	0.0	0.0	0.0	18.628486581829414	0.0
33	0.0	0.0	0.0	19.115785907183927	0.0
34	0.0	0.0	0.0	19.543792165342953	0.0
35	0.0	0.0	0.0	20.033582796041813	0.0
36	0.0	0.0	0.0	20.507429072536848	0.0
37	0.0	0.0	0.0	20.946895335279873	0.0
38	0.0	0.0	0.0	21.408285085053166	0.0
39	0.0	0.0	0.0	21.869176573757585	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGGC	35	1.205226E-7	45.000004	4
GCCCTAC	45	3.8198777E-10	45.000004	26
AGCTACG	35	1.205226E-7	45.000004	9
CCACGGG	35	1.205226E-7	45.000004	3
CACGGGT	25	3.879265E-5	45.0	4
CGTGCGG	20	7.0193585E-4	45.0	2
ATAGCGG	20	7.0193585E-4	45.0	2
CTCGGTT	20	7.0193585E-4	45.0	11
GCGATCT	20	7.0193585E-4	45.0	9
CGGTAGT	20	7.0193585E-4	45.0	12
CGTTTTA	20	7.0193585E-4	45.0	1
GACCTTA	25	3.879265E-5	45.0	9
ACGACGG	20	7.0193585E-4	45.0	2
TAGTACG	20	7.0193585E-4	45.0	1
GGTATGC	30	2.156521E-6	44.999996	8
AGGCGAT	95	0.0	42.63158	7
CCCTACA	50	1.0713848E-9	40.5	27
GGCGATC	50	1.0713848E-9	40.5	8
AATGGGC	40	3.439236E-7	39.375	4
CTACGAA	40	3.439236E-7	39.375	11
>>END_MODULE