##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936561.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258813
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96116887482468	33.0	31.0	34.0	30.0	34.0
2	32.09018866903904	33.0	31.0	34.0	30.0	34.0
3	31.58097158952603	33.0	31.0	34.0	28.0	34.0
4	35.53185504592118	37.0	35.0	37.0	33.0	37.0
5	35.75614826148609	37.0	35.0	37.0	35.0	37.0
6	35.790516705111415	37.0	35.0	37.0	35.0	37.0
7	26.960763176501953	35.0	11.0	37.0	0.0	37.0
8	31.281029160049922	37.0	21.0	37.0	17.0	37.0
9	36.06723000776623	39.0	32.0	39.0	32.0	39.0
10	36.77425013426683	38.0	35.0	39.0	33.0	39.0
11	37.130298709879334	39.0	37.0	39.0	34.0	39.0
12	37.26682199116737	39.0	37.0	39.0	34.0	39.0
13	37.301572950354114	39.0	37.0	39.0	34.0	39.0
14	38.60050306592018	40.0	38.0	41.0	35.0	41.0
15	38.65655511894688	40.0	38.0	41.0	35.0	41.0
16	38.56493298250088	40.0	38.0	41.0	34.0	41.0
17	38.464938005432494	40.0	38.0	41.0	34.0	41.0
18	38.28546479504507	40.0	38.0	41.0	34.0	41.0
19	38.0918075985364	40.0	37.0	41.0	34.0	41.0
20	38.041929887602244	40.0	36.0	41.0	34.0	41.0
21	37.9751094419523	40.0	36.0	41.0	33.0	41.0
22	37.96570496845212	40.0	36.0	41.0	34.0	41.0
23	37.88813544914668	40.0	36.0	41.0	33.0	41.0
24	37.81905854806366	40.0	36.0	41.0	33.0	41.0
25	37.70212856386657	40.0	35.0	41.0	33.0	41.0
26	37.61465227789949	40.0	35.0	41.0	33.0	41.0
27	37.51449115770846	40.0	35.0	41.0	33.0	41.0
28	37.49363440012673	40.0	35.0	41.0	33.0	41.0
29	37.441036578533534	40.0	35.0	41.0	33.0	41.0
30	37.328155849976625	39.0	35.0	41.0	33.0	41.0
31	37.17860772063227	39.0	35.0	41.0	32.0	41.0
32	36.95252170486027	39.0	35.0	41.0	31.0	41.0
33	36.72324033182259	39.0	35.0	41.0	31.0	41.0
34	36.394999478387874	39.0	35.0	41.0	30.0	41.0
35	36.187648997538766	39.0	35.0	41.0	29.0	41.0
36	35.9457639299417	39.0	35.0	41.0	27.0	41.0
37	35.83252386858465	39.0	35.0	41.0	26.0	41.0
38	35.68244639952398	39.0	35.0	41.0	25.0	41.0
39	35.61769308342317	39.0	35.0	41.0	25.0	41.0
40	35.49873460761245	39.0	35.0	41.0	25.0	41.0
41	35.39524676117506	39.0	35.0	40.0	24.0	41.0
42	35.31631332274654	39.0	35.0	40.0	24.0	41.0
43	35.29787529992697	39.0	35.0	40.0	24.0	41.0
44	35.10063636679765	39.0	35.0	40.0	23.0	41.0
45	35.00734507153814	38.0	34.0	40.0	23.0	41.0
46	34.905553430469105	38.0	34.0	40.0	23.0	41.0
47	34.79909819058548	38.0	34.0	40.0	23.0	41.0
48	34.71009570616623	38.0	34.0	40.0	22.0	41.0
49	34.592253866691394	38.0	34.0	40.0	22.0	41.0
50	34.38161143373787	38.0	34.0	40.0	21.0	41.0
51	32.510260303771446	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	15.0
10	12.0
11	14.0
12	8.0
13	11.0
14	4.0
15	20.0
16	32.0
17	51.0
18	105.0
19	184.0
20	323.0
21	553.0
22	882.0
23	1357.0
24	2179.0
25	3268.0
26	4307.0
27	4708.0
28	4585.0
29	4724.0
30	5086.0
31	6058.0
32	7665.0
33	10069.0
34	15316.0
35	19943.0
36	22698.0
37	37075.0
38	49717.0
39	57821.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.87522265110331	19.581705710300486	23.566435998191746	18.976635640404464
2	35.45880616506899	23.322244245845454	25.327939477537836	15.891010111547722
3	30.18550072832508	22.88988574762473	31.316046720991604	15.60856680305858
4	26.70576825739047	24.218257970040145	31.73140452759328	17.344569244976103
5	23.751511709226353	29.004339040156406	29.609409110052432	17.63474014056481
6	24.911422532871224	33.32715126365368	28.37685896767164	13.384567235803457
7	58.7729364444599	27.11687589108739	10.589498981890399	3.520688682562313
8	80.93179245246567	4.036118742103372	12.030693975959476	3.001394829471472
9	74.59323913404658	7.20017928002071	13.740809001093455	4.465772584839247
10	36.5823973293459	30.312233156757966	20.367214938971383	12.738154574924753
11	26.040036628762852	25.26611878074131	31.949708863156022	16.744135727339817
12	24.439653340442714	23.35044993875887	33.68223389087874	18.527662829919674
13	21.906936668559926	23.89022189766356	35.23934269144131	18.9634987423352
14	19.602956574824294	26.25988648174551	34.385830696294235	19.751326247135964
15	19.011796161707487	25.53619794987114	36.76283648812076	18.689169400300603
16	22.000054093109696	24.993721335481602	34.717344182865624	18.28888038854308
17	22.440140178429985	24.564453872100707	33.1826453848918	19.812760564577513
18	22.025168751183287	25.11543083229977	33.030025539675364	19.82937487684158
19	22.98184403410957	26.64897049220866	31.420755526190725	18.948429947491046
20	23.49688771429565	26.046991457152462	32.01462059479238	18.44150023375951
21	23.330744591654977	25.77150297705293	32.424182711069385	18.47356972022271
22	22.0356009937677	24.44544903076739	32.31792838845035	21.201021587014562
23	20.682500492633675	25.878143679026945	33.166031072627725	20.273324755711652
24	20.867576203668285	25.23173101814824	34.02456599938952	19.876126778793957
25	20.57276875581984	26.64897049220866	32.297836661991475	20.480424089980023
26	20.255937684737628	27.26679108081897	31.952027139285892	20.525244095157507
27	20.18948043568136	26.51605599409612	32.919907423506544	20.374556146715967
28	19.262556363088407	26.623469454780093	33.75757786509951	20.356396317031987
29	20.53413082032201	26.103016463624318	32.911793457052	20.45105925900167
30	21.02831001533926	25.740979008009646	32.81172120411262	20.418989772538474
31	21.38416540127428	26.2208621668927	31.406845869411505	20.988126562421517
32	21.588560080057803	27.552325424147938	30.904166328584733	19.95494816720953
33	21.468009721304572	26.666357563182686	30.94550891956741	20.920123795945337
34	20.066998180153238	27.757106482286435	31.825294710853008	20.350600626707312
35	20.348282350577442	27.507119039615475	30.985305993130176	21.159292616676908
36	20.61179307067265	28.596708820654293	29.978015014701736	20.81348309397132
37	20.209185782785255	28.945995757554684	30.318415226437622	20.526403233222442
38	20.782959124928038	28.52947881288807	29.891079659831615	20.79648240235228
39	20.861394133988632	27.519869558329756	29.610954627472346	22.007781680209263
40	22.093944276369427	27.096397785273535	30.142226240567517	20.667431697789524
41	19.536499325768027	27.907021672018022	30.05645002376233	22.50002897845162
42	20.393102355754927	28.008639442377316	29.77477947398315	21.823478727884613
43	21.241591419287285	26.83790999679305	30.029789848268827	21.890708735650836
44	20.537221855161835	27.794585279719335	29.99347018890087	21.674722676217964
45	20.71804739329168	27.31392936212632	29.76743826623856	22.20058497834344
46	20.62724824487178	28.00091185527775	29.370626668675836	22.00121323117463
47	20.36914683574627	27.25094952726486	30.55372025361941	21.82618338336946
48	20.302303207335026	26.634288076719486	30.82689045758907	22.23651825835642
49	20.50631150676357	26.432984432775786	30.637950953004676	22.42275310745596
50	19.446472936058083	27.033031571057098	30.69513509754147	22.825360395343356
51	19.910900920742	26.48050909343812	30.021289502459304	23.587300483360572
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	267.0
2	431.0
3	3554.0
4	6677.0
5	4636.0
6	2595.0
7	2256.0
8	1917.0
9	1800.5
10	1684.0
11	1657.5
12	1631.0
13	1578.5
14	1526.0
15	1455.5
16	1385.0
17	1383.0
18	1381.0
19	1370.5
20	1360.0
21	1404.0
22	1448.0
23	1575.0
24	1702.0
25	2125.5
26	2867.5
27	3186.0
28	3813.0
29	4440.0
30	5095.5
31	5751.0
32	6598.5
33	7446.0
34	8150.5
35	8855.0
36	9705.5
37	10556.0
38	11340.0
39	12124.0
40	12965.0
41	13806.0
42	14818.0
43	15830.0
44	16519.5
45	17209.0
46	18646.5
47	20084.0
48	19699.0
49	19314.0
50	18540.0
51	17766.0
52	16310.5
53	14855.0
54	13424.0
55	11993.0
56	11165.0
57	10337.0
58	9891.5
59	9446.0
60	8850.5
61	8255.0
62	7465.5
63	6676.0
64	5675.5
65	4675.0
66	3868.0
67	3061.0
68	2684.0
69	2307.0
70	1943.5
71	1580.0
72	1328.0
73	1076.0
74	914.0
75	629.0
76	506.0
77	391.0
78	276.0
79	210.0
80	144.0
81	102.5
82	61.0
83	46.0
84	31.0
85	22.5
86	14.0
87	9.0
88	4.0
89	3.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258813.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.97887373965122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73202542215547	35.54860119606213
2	11.498817828392612	11.72393564457755
3	6.164714681525553	9.42810634171396
4	3.8089914016259376	7.767123669556233
5	2.646811688816754	6.746573944841117
6	1.8391505630599432	5.625469460546478
7	1.3288203056934567	4.741923381064203
8	0.9242461166994638	3.769362087006793
9	0.6478991590992751	2.9726252484973332
>10	1.3858170605491893	8.895157032001482
>50	0.01210974527057915	0.39432667563868334
>100	0.009082308952934362	0.8376548070563419
>500	7.568590794111968E-4	0.24423560242591638
>1k	7.568590794111968E-4	1.3049049090117681
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3382	1.3067349785366267	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	633	0.24457813170126694	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	344	0.13291449811253683	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.863793549783048E-4	0.0	0.0	0.05332035098700606	0.0
2	3.863793549783048E-4	0.0	0.0	0.21212226588308933	0.0
3	3.863793549783048E-4	0.0	0.0	0.34928693690038753	0.0
4	3.863793549783048E-4	0.0	0.0	0.4937928156622735	0.0
5	3.863793549783048E-4	0.0	0.0	0.9524251100215213	0.0
6	3.863793549783048E-4	0.0	0.0	1.4010115411513333	0.0
7	3.863793549783048E-4	0.0	0.0	1.7688446870906793	0.0
8	3.863793549783048E-4	0.0	0.0	2.485578390575435	0.0
9	3.863793549783048E-4	0.0	0.0	2.903254473306982	0.0
10	3.863793549783048E-4	0.0	0.0	3.6006692090428225	0.0
11	3.863793549783048E-4	0.0	0.0	4.164396687956169	0.0
12	3.863793549783048E-4	0.0	0.0	4.680985885562163	0.0
13	7.727587099566096E-4	0.0	0.0	4.9135862572591025	0.0
14	7.727587099566096E-4	0.0	0.0	5.039545926982029	0.0
15	7.727587099566096E-4	0.0	0.0	5.174392321869458	0.0
16	7.727587099566096E-4	0.0	0.0	5.433266489704922	0.0
17	7.727587099566096E-4	0.0	0.0	5.757052389176741	0.0
18	7.727587099566096E-4	0.0	0.0	6.135317777700502	0.0
19	7.727587099566096E-4	0.0	0.0	6.359804182942897	0.0
20	7.727587099566096E-4	0.0	0.0	6.569221793341138	0.0
21	7.727587099566096E-4	0.0	0.0	6.8389145831159945	0.0
22	7.727587099566096E-4	0.0	0.0	7.121357891605136	0.0
23	7.727587099566096E-4	0.0	0.0	7.419642753648387	0.0
24	7.727587099566096E-4	0.0	0.0	7.67194847244922	0.0
25	7.727587099566096E-4	0.0	0.0	7.887161773172136	0.0
26	7.727587099566096E-4	0.0	0.0	8.103920591314965	0.0
27	7.727587099566096E-4	0.0	0.0	8.313338201713206	0.0
28	7.727587099566096E-4	0.0	0.0	8.547870470185037	0.0
29	7.727587099566096E-4	0.0	0.0	8.783561876721803	0.0
30	7.727587099566096E-4	0.0	0.0	9.074891910375445	0.0
31	7.727587099566096E-4	0.0	0.0	9.338402630470648	0.0
32	7.727587099566096E-4	0.0	0.0	9.574866795717371	0.0
33	7.727587099566096E-4	0.0	0.0	9.82833165258314	0.0
34	7.727587099566096E-4	0.0	0.0	10.072909784284406	0.0
35	7.727587099566096E-4	0.0	0.0	10.322510847600391	0.0
36	7.727587099566096E-4	0.0	0.0	10.60070398318477	0.0
37	7.727587099566096E-4	0.0	0.0	10.858805392310279	0.0
38	7.727587099566096E-4	0.0	0.0	11.148976287898986	0.0
39	7.727587099566096E-4	0.0	0.0	11.447647529297214	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	20	7.023149E-4	45.0	5
TACGGGT	20	7.023149E-4	45.0	4
CGACACC	20	7.023149E-4	45.0	35
AGGCGAT	135	0.0	45.0	7
AATGCGG	20	7.023149E-4	45.0	2
TTAAGCC	20	7.023149E-4	45.0	10
TCGACAC	20	7.023149E-4	45.0	34
CGTTTGG	20	7.023149E-4	45.0	2
CGAAGGC	20	7.023149E-4	45.0	4
CGTTTTA	205	0.0	43.90244	1
CGTTTTT	1590	0.0	43.301888	1
CGGGAAT	65	0.0	41.538464	6
GGTATGC	45	1.9183062E-8	40.000004	8
TACACGC	45	1.9183062E-8	40.000004	35
ACACGCG	40	3.444402E-7	39.375	36
TACGAAT	40	3.444402E-7	39.375	12
CGAATAT	40	3.444402E-7	39.375	14
CTGTTAT	35	6.2295458E-6	38.571426	23
CGGGATC	35	6.2295458E-6	38.571426	6
TGCGGGC	65	9.094947E-12	38.076927	4
>>END_MODULE