##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936560.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	256066
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15272234502042	33.0	31.0	34.0	30.0	34.0
2	32.30224629587684	33.0	31.0	34.0	30.0	34.0
3	32.15578015042997	33.0	31.0	34.0	30.0	34.0
4	35.891707606632664	37.0	35.0	37.0	35.0	37.0
5	35.95819437176353	37.0	35.0	37.0	35.0	37.0
6	35.92569493802379	37.0	35.0	37.0	35.0	37.0
7	26.88212023462701	36.0	10.0	37.0	0.0	37.0
8	31.21579592761241	36.0	19.0	37.0	17.0	37.0
9	36.09235119070865	39.0	32.0	39.0	32.0	39.0
10	36.826798559746315	38.0	37.0	39.0	34.0	39.0
11	37.14632946193559	39.0	37.0	39.0	34.0	39.0
12	37.18131653558067	39.0	37.0	39.0	34.0	39.0
13	37.14616934696524	39.0	37.0	39.0	34.0	39.0
14	38.35772027524154	40.0	38.0	41.0	34.0	41.0
15	38.45086032507244	40.0	38.0	41.0	34.0	41.0
16	38.39642904563667	40.0	38.0	41.0	34.0	41.0
17	38.27558129544727	40.0	37.0	41.0	34.0	41.0
18	38.18730717861801	40.0	37.0	41.0	34.0	41.0
19	38.06544015995876	40.0	37.0	41.0	34.0	41.0
20	38.09125381737521	40.0	37.0	41.0	34.0	41.0
21	38.01992845594495	40.0	37.0	41.0	34.0	41.0
22	38.01802269727336	40.0	37.0	41.0	34.0	41.0
23	37.93106855263877	40.0	36.0	41.0	33.0	41.0
24	37.86677653417478	40.0	36.0	41.0	33.0	41.0
25	37.73556036334382	40.0	36.0	41.0	33.0	41.0
26	37.623593136144585	40.0	36.0	41.0	33.0	41.0
27	37.50310857357088	40.0	35.0	41.0	33.0	41.0
28	37.447771277717464	40.0	35.0	41.0	33.0	41.0
29	37.392027836573384	40.0	35.0	41.0	33.0	41.0
30	37.26483016097412	39.0	35.0	41.0	32.0	41.0
31	37.105968773675535	39.0	35.0	41.0	32.0	41.0
32	36.95221934969891	39.0	35.0	41.0	31.0	41.0
33	36.813020861809065	39.0	35.0	41.0	31.0	41.0
34	36.614286941647855	39.0	35.0	41.0	30.0	41.0
35	36.47527200018745	39.0	35.0	41.0	30.0	41.0
36	36.253829090937494	39.0	35.0	41.0	30.0	41.0
37	36.16991322549655	39.0	35.0	41.0	29.0	41.0
38	36.0023040934759	39.0	35.0	40.0	29.0	41.0
39	35.93974990822679	39.0	35.0	40.0	29.0	41.0
40	35.736161770793466	38.0	35.0	40.0	28.0	41.0
41	35.632087040059986	38.0	35.0	40.0	27.0	41.0
42	35.52570431060742	38.0	35.0	40.0	27.0	41.0
43	35.439730382010886	38.0	34.0	40.0	27.0	41.0
44	35.219006037505956	38.0	34.0	40.0	26.0	41.0
45	35.08789140299766	38.0	34.0	40.0	26.0	41.0
46	34.9788999711012	38.0	34.0	40.0	26.0	41.0
47	34.83454656221443	37.0	34.0	40.0	25.0	41.0
48	34.768622152101415	37.0	34.0	40.0	25.0	41.0
49	34.58239672584412	37.0	34.0	40.0	24.0	41.0
50	34.372072043926174	37.0	33.0	40.0	24.0	41.0
51	32.341599431396595	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	19.0
9	28.0
10	26.0
11	21.0
12	12.0
13	14.0
14	16.0
15	21.0
16	49.0
17	58.0
18	148.0
19	213.0
20	332.0
21	514.0
22	764.0
23	1167.0
24	1668.0
25	2274.0
26	2801.0
27	3134.0
28	3612.0
29	4221.0
30	4973.0
31	6229.0
32	8074.0
33	11570.0
34	18600.0
35	22815.0
36	25363.0
37	39214.0
38	47842.0
39	50266.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.386595643310706	19.890184561792662	24.995508970343582	13.727710824553045
2	30.77487835167496	24.86936961564597	26.716159115228105	17.63959291745097
3	32.708364249841836	23.418181250146446	27.311318175782805	16.56213632422891
4	28.482110080994744	26.408425952684073	26.31743378660189	18.79203017971929
5	25.652370873134274	30.642490607890156	24.88928635586138	18.815852163114197
6	26.62790061937157	34.885537322409064	23.748564823131538	14.73799723508783
7	63.22315340576258	27.8818742043067	5.324018026602516	3.570954363328204
8	86.64953566658595	4.408238501011458	5.961744237813689	2.9804815945888947
9	81.23843071708076	6.408113533229715	7.826107331703545	4.527348417985988
10	46.30798309810752	24.36988901298884	14.568119156779893	14.754008732123749
11	32.31510626166691	24.635836073512298	23.25923785274109	19.7898198120797
12	29.001507423867285	22.099771152749682	28.597705279107732	20.301016144275305
13	24.804152054548435	23.982098365265205	29.701327001632393	21.512422578553966
14	21.742441401826092	24.208602469675785	30.906485046823867	23.142471081674255
15	21.314036225035732	22.95033311724321	33.25626986792468	22.47936078979638
16	25.92339474979107	22.41062850983731	29.617754797591246	22.048221942780376
17	26.197152296673515	23.163559394843517	27.14729796224411	23.49199034623886
18	26.674763537525482	23.127631157592184	27.462841611147127	22.734763693735207
19	26.94656846281818	25.18178907000539	25.079862223020626	22.791780244155806
20	28.374325369240744	24.84515710793311	25.954636695227013	20.825880827599136
21	27.76354533596807	25.222794123390063	26.447868908796952	20.565791631844917
22	28.254043879312363	22.8195074707302	25.704701131739473	23.22174751821796
23	26.424046925402045	23.577515171869752	26.69585185069474	23.302586052033462
24	25.294650597892733	23.180742464833283	28.872634398944026	22.65197253832996
25	25.557082939554647	24.368717440034988	26.301031765248023	23.77316785516234
26	24.339428116188795	25.194285848179764	25.53989986956488	24.92638616606656
27	23.729038607234074	25.07048963938984	26.831754313341094	24.368717440034988
28	22.029867299836763	25.75585981739083	26.993431380972094	25.22084150180032
29	23.924691290526663	24.8244593190818	27.285153046480204	23.965696343911336
30	25.107589449595025	24.647161278732828	26.597439722571526	23.647809549100625
31	25.67228761334968	25.487569610959675	25.800379589637046	23.039763186053598
32	27.420274460490656	24.53234712925574	24.797122616825348	23.250255793428256
33	26.78645349245898	24.71159779119446	24.571009036732715	23.93093967961385
34	24.720970374825242	24.951770246733265	26.885646669218094	23.441612709223403
35	25.06267915303086	25.72657049354463	26.754821022705084	22.455929330719425
36	25.624643646559868	27.761983238696274	24.32068294892723	22.292690165816627
37	24.654971765091812	27.62920497059352	24.800637335686893	22.915185928627775
38	24.49837151359415	27.278904657393017	25.594182749759824	22.628541079253004
39	24.225395015347605	26.575179836448413	25.20443948044645	23.994985667757533
40	26.19129443190428	24.97090593831278	25.958151414088555	22.87964821569439
41	22.8472346973046	24.985355338076904	26.607202830520254	25.56020713409824
42	24.003967727070364	25.220060453164418	25.68478439152406	25.091187428241156
43	23.046792623776682	24.670202213491834	27.008661829372116	25.27434333335937
44	23.597822436403113	24.682308467348264	27.09262455773121	24.627244538517413
45	24.13596494653722	25.00839627283591	25.86950239391407	24.9861363867128
46	24.36285957526575	25.47390125983145	25.80467535713449	24.35856380776831
47	22.848796794576398	25.62620574383167	27.528059172244657	23.996938289347277
48	23.668897862269883	24.947083954917872	27.422617606398354	23.961400576413894
49	23.674365202721173	25.250911874282412	27.45346902751634	23.62125389548007
50	23.027266407879218	25.57036076636492	27.7994735732194	23.602899252536456
51	22.742183655776245	25.429381487585236	27.343341169854646	24.485093686783877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	93.0
1	276.5
2	460.0
3	1207.5
4	1955.0
5	1334.0
6	713.0
7	644.0
8	575.0
9	563.5
10	552.0
11	553.0
12	554.0
13	533.5
14	513.0
15	535.5
16	558.0
17	552.0
18	546.0
19	558.0
20	570.0
21	632.5
22	695.0
23	729.5
24	764.0
25	1005.0
26	1515.5
27	1785.0
28	2242.0
29	2699.0
30	3274.5
31	3850.0
32	4725.5
33	5601.0
34	6552.0
35	7503.0
36	7610.0
37	7717.0
38	8421.5
39	9126.0
40	10116.0
41	11106.0
42	11654.0
43	12202.0
44	12865.5
45	13529.0
46	14156.5
47	14784.0
48	15300.5
49	15817.0
50	16170.5
51	16524.0
52	16037.5
53	15551.0
54	15364.5
55	15178.0
56	15274.5
57	15371.0
58	15304.5
59	15238.0
60	15033.5
61	14829.0
62	13889.5
63	12950.0
64	11624.5
65	10299.0
66	9037.0
67	7775.0
68	6793.5
69	5812.0
70	4988.5
71	4165.0
72	3471.5
73	2778.0
74	2263.0
75	1420.0
76	1092.0
77	834.5
78	577.0
79	445.0
80	313.0
81	235.5
82	158.0
83	116.0
84	74.0
85	65.5
86	57.0
87	43.0
88	29.0
89	22.0
90	15.0
91	13.0
92	11.0
93	7.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	256066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.03488928646178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.35206662400133	24.765403721250596
2	12.329454543542372	10.11875604313449
3	7.5015434218305	9.234750113772009
4	5.136281753329784	8.430670123678457
5	3.7162829911683493	7.624863054987712
6	2.845010708023662	7.004681965352953
7	2.1115557986701123	6.06532208944301
8	1.6422067059861305	5.391021629250076
9	1.2216897358238488	4.511871274674454
>10	3.132946391233069	15.96942260250459
>50	0.006975389521472952	0.1766474763903287
>100	0.0029894526520598363	0.3263071438876905
>500	9.964842173532788E-4	0.3802827616736243
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	930	0.3631876156928292	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	493	0.1925284887489944	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03553771293338436	0.0
2	0.0	0.0	0.0	0.14215085173353745	0.0
3	0.0	0.0	0.0	0.20580631555926987	0.0
4	0.0	0.0	0.0	0.2803964602875821	0.0
5	0.0	0.0	0.0	0.4893269703904462	0.0
6	0.0	0.0	0.0	0.6982574804933103	0.0
7	0.0	0.0	0.0	0.8860996774269134	0.0
8	0.0	0.0	0.0	1.305913319222388	0.0
9	0.0	0.0	0.0	1.5054712456944694	0.0
10	0.0	0.0	0.0	1.8674872884334508	0.0
11	0.0	0.0	0.0	2.2767567736442946	0.0
12	0.0	0.0	0.0	2.603235103449892	0.0
13	0.0	0.0	0.0	2.7481196254090743	0.0
14	0.0	0.0	0.0	2.819585575593792	0.0
15	0.0	0.0	0.0	2.9277608116657423	0.0
16	0.0	0.0	0.0	3.1390344676763022	0.0
17	0.0	0.0	0.0	3.3624143775432898	0.0
18	0.0	0.0	0.0	3.657260237594995	0.0
19	0.0	0.0	0.0	3.826747791584982	0.0
20	0.0	0.0	0.0	3.993501675349324	0.0
21	0.0	0.0	0.0	4.20711847726758	0.0
22	0.0	0.0	0.0	4.431279435770465	0.0
23	0.0	0.0	0.0	4.64528676200667	0.0
24	0.0	0.0	0.0	4.8343005318941215	0.0
25	0.0	0.0	0.0	5.022923777463623	0.0
26	0.0	0.0	0.0	5.188115563956168	0.0
27	0.0	0.0	0.0	5.374005139300024	0.0
28	0.0	0.0	0.0	5.553255801238743	0.0
29	0.0	0.0	0.0	5.736411706356955	0.0
30	0.0	0.0	0.0	5.956667421680348	0.0
31	0.0	0.0	0.0	6.159349542696024	0.0
32	0.0	0.0	0.0	6.358516944850156	0.0
33	0.0	0.0	0.0	6.562370638819679	0.0
34	0.0	0.0	0.0	6.7814547811892245	0.0
35	0.0	0.0	0.0	7.002101020830567	0.0
36	0.0	0.0	0.0	7.233681941374489	0.0
37	0.0	0.0	0.0	7.46604391055431	0.0
38	0.0	0.0	0.0	7.707387939046964	0.0
39	0.0	0.0	0.0	8.035428366124359	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCACG	20	7.0230075E-4	45.0	23
GACAATT	20	7.0230075E-4	45.0	9
CGCATCG	25	3.8822876E-5	45.0	21
TAACGCC	25	3.8822876E-5	45.0	12
TCGATCA	25	3.8822876E-5	45.0	17
AATGACG	20	7.0230075E-4	45.0	1
GCGATAC	20	7.0230075E-4	45.0	9
TAAGGCG	20	7.0230075E-4	45.0	5
TAGGCTC	35	1.2069904E-7	45.0	6
ACACGAC	30	2.158873E-6	44.999996	26
TACGGGT	30	2.158873E-6	44.999996	4
CGTTTTA	60	3.6379788E-12	41.249996	1
AACGGGA	55	6.002665E-11	40.909092	4
ATTAGGA	45	1.9183062E-8	40.000004	4
GGGTAAC	40	3.444202E-7	39.375	7
TATGCCA	80	0.0	39.375	10
CACGACC	35	6.2292856E-6	38.571426	27
CGAGACA	35	6.2292856E-6	38.571426	22
TGTACGG	35	6.2292856E-6	38.571426	2
CGTTTTT	370	0.0	37.7027	1
>>END_MODULE