##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936559.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197419
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.003079744097576	33.0	31.0	34.0	30.0	34.0
2	32.112020626180865	33.0	31.0	34.0	30.0	34.0
3	31.896965337682797	33.0	31.0	34.0	30.0	34.0
4	35.6881201910657	37.0	35.0	37.0	35.0	37.0
5	35.804643929915564	37.0	35.0	37.0	35.0	37.0
6	35.78188522887868	37.0	35.0	37.0	35.0	37.0
7	26.622402099088742	35.0	10.0	37.0	0.0	37.0
8	30.97599521829206	35.0	19.0	37.0	17.0	37.0
9	35.83528434446532	39.0	32.0	39.0	32.0	39.0
10	36.632558163094735	38.0	35.0	39.0	33.0	39.0
11	37.01300280114882	39.0	37.0	39.0	34.0	39.0
12	37.08151191121422	39.0	37.0	39.0	34.0	39.0
13	37.05520745217026	39.0	37.0	39.0	33.0	39.0
14	38.3625740176984	40.0	38.0	41.0	34.0	41.0
15	38.37330246835411	40.0	38.0	41.0	34.0	41.0
16	38.335008281877634	40.0	38.0	41.0	34.0	41.0
17	38.2115044651224	40.0	37.0	41.0	33.0	41.0
18	38.07671500716749	40.0	37.0	41.0	33.0	41.0
19	37.894062881485574	40.0	37.0	41.0	33.0	41.0
20	37.956665771784884	40.0	37.0	41.0	33.0	41.0
21	37.98917024197266	40.0	37.0	41.0	33.0	41.0
22	37.98332987199814	40.0	37.0	41.0	33.0	41.0
23	37.981643104260485	40.0	37.0	41.0	34.0	41.0
24	37.881227237499935	40.0	36.0	41.0	33.0	41.0
25	37.80886844731257	40.0	36.0	41.0	33.0	41.0
26	37.6336117597597	40.0	36.0	41.0	33.0	41.0
27	37.544451141987345	40.0	36.0	41.0	33.0	41.0
28	37.534842137788154	40.0	36.0	41.0	33.0	41.0
29	37.49316935046779	40.0	36.0	41.0	33.0	41.0
30	37.410188482364916	40.0	36.0	41.0	33.0	41.0
31	37.29687112182718	40.0	36.0	41.0	32.0	41.0
32	37.14711856508239	40.0	35.0	41.0	32.0	41.0
33	37.00543007511941	39.0	35.0	41.0	31.0	41.0
34	36.859258733961774	39.0	35.0	41.0	31.0	41.0
35	36.74096718147696	39.0	35.0	41.0	31.0	41.0
36	36.523434927742514	39.0	35.0	41.0	30.0	41.0
37	36.39012455741342	39.0	35.0	41.0	30.0	41.0
38	36.21463992827438	39.0	35.0	41.0	29.0	41.0
39	36.053672645490046	39.0	35.0	41.0	29.0	41.0
40	35.77415547642324	39.0	35.0	40.0	27.0	41.0
41	35.68132246642927	39.0	35.0	40.0	26.0	41.0
42	35.572102989074	39.0	35.0	40.0	26.0	41.0
43	35.496213636985296	38.0	35.0	40.0	26.0	41.0
44	35.28214609536063	38.0	34.0	40.0	25.0	41.0
45	35.16016188917987	38.0	34.0	40.0	24.0	41.0
46	34.85412245022009	38.0	34.0	40.0	23.0	41.0
47	34.65260689194049	38.0	34.0	40.0	23.0	41.0
48	34.56567503634402	38.0	34.0	40.0	22.0	41.0
49	34.405842396121955	38.0	34.0	40.0	22.0	41.0
50	34.26404753341877	38.0	33.0	40.0	22.0	41.0
51	32.02793044235864	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	17.0
10	25.0
11	13.0
12	5.0
13	7.0
14	5.0
15	12.0
16	38.0
17	60.0
18	117.0
19	140.0
20	232.0
21	357.0
22	548.0
23	912.0
24	1290.0
25	1938.0
26	2540.0
27	2879.0
28	3192.0
29	3596.0
30	4276.0
31	5050.0
32	6449.0
33	8659.0
34	12862.0
35	16164.0
36	18362.0
37	28817.0
38	37281.0
39	41555.0
40	10.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.24792446522371	19.193694629189693	23.22876724124831	12.329613664338286
2	32.34187185630562	27.771389785177718	24.107608690146336	15.77912966837032
3	30.533028735835966	27.50697754522108	26.580521631656527	15.37947208728643
4	27.344885750611642	24.347707160911565	30.106018164411736	18.20138892406506
5	26.246713842132724	29.3963600261373	25.2665650216038	19.090361110126178
6	25.70319979333296	36.873857126213785	24.839047913321412	12.583895167131837
7	58.8651548229907	28.344789508608592	9.114624225631777	3.675431442768933
8	79.25883526914836	7.58741559829601	9.45147123630451	3.702277896251121
9	73.33286056559906	7.813837573891064	10.457959973457468	8.395341887052412
10	40.13747410330313	25.48944123919177	19.266635936763937	15.106448720741165
11	31.804436249803718	26.088674342388522	24.201824545763074	17.905064862044686
12	28.296161970225764	22.998799507646172	29.262634295584515	19.442404226543545
13	23.68059811872211	24.862855145654674	31.883455999675814	19.573090735947403
14	20.971132464453778	29.238827063251254	28.096586448112898	21.693454024182067
15	19.927159999797386	26.44426321681297	34.49161428231326	19.13696250107639
16	22.32814470744964	25.140943880781485	30.706264341324797	21.82464707044408
17	22.77693636377451	24.229177536103414	30.187570598574602	22.80631550154747
18	23.619307158885416	26.51872413496168	28.345802582324904	21.516166123828
19	24.875518567108536	27.41732052132774	26.66004791838678	21.04711299317695
20	26.82669854471961	26.17529214513294	28.886783946833894	18.11122536331356
21	25.50413080807825	26.876339156818744	27.45733693312194	20.162193101981067
22	23.309813138553025	25.476777817737904	27.31601314969684	23.897395894012227
23	23.557509662190572	28.141668228488648	28.297681580800226	20.003140528520557
24	23.32703539173028	25.142463491355947	28.088481858382426	23.442019258531346
25	22.274958337343417	29.323925255421212	27.051094372882044	21.35002203435333
26	22.143765291081404	28.113302164431996	27.319558907703918	22.42337363678268
27	23.632477117197435	28.702911067323818	26.4528743434016	21.211737472077154
28	21.47868239632457	27.497859881774296	29.829449039859384	21.194008682041748
29	23.218636504085218	26.74008074197519	28.880198967677885	21.16108378626171
30	22.450220090264867	28.763695490302354	27.69388964588008	21.0921947735527
31	25.319751391710017	26.626616485748585	26.112481574721784	21.941150547819614
32	25.685977540155708	28.564119968189484	25.872383103956558	19.877519387698246
33	23.392378646432206	28.961751401840758	25.436254869085552	22.209615082641488
34	23.348816476630923	27.4244120373419	28.683662666713943	20.54310881931324
35	23.070221204645954	26.66663289754279	26.562792841621118	23.700353056190135
36	24.878557788257464	28.2601978532968	26.561273231046655	20.299971127399086
37	23.887771693707293	29.157274629088388	26.905718294591708	20.049235382612615
38	23.963752222430465	28.257158632147867	25.02038810854072	22.75870103688095
39	25.486908554901	25.696108277318796	27.460376154270865	21.356607013509336
40	22.521135250406495	27.39199367842001	28.62085209630279	21.466018974870707
41	21.85503928193335	27.56472274705069	28.730264057664158	21.849973913351807
42	22.673602844710995	27.088578100385476	28.331619550296576	21.906199504606953
43	25.506156955510868	26.789214817216173	26.227465441522853	21.477162785750107
44	23.39592440443929	27.53078477755434	26.971568086151787	22.101722731854583
45	22.324092412584402	26.42349520562864	26.64181259149322	24.61059979029374
46	23.365025656091866	28.54183234643069	25.932660990076943	22.160481007400502
47	22.68525319244855	27.027287140548783	29.712945562483856	20.574514104518816
48	23.423783931637786	26.21074972520375	26.845440408471323	23.52002593468714
49	21.01773385540399	26.17225292398401	29.633419275753596	23.176593944858396
50	21.010642339389825	26.496943050061038	28.645165865494203	23.847248745054934
51	21.57745708366469	25.786778374928453	27.114918017009508	25.52084652439735
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	246.0
2	421.0
3	1122.5
4	1824.0
5	1354.0
6	884.0
7	749.0
8	614.0
9	608.0
10	602.0
11	612.5
12	623.0
13	587.5
14	552.0
15	605.5
16	659.0
17	637.0
18	615.0
19	652.5
20	690.0
21	758.0
22	826.0
23	896.5
24	967.0
25	1212.0
26	1712.0
27	1967.0
28	2286.0
29	2605.0
30	3145.0
31	3685.0
32	4147.0
33	4609.0
34	5218.5
35	5828.0
36	6173.5
37	6519.0
38	7049.0
39	7579.0
40	8678.0
41	9777.0
42	10671.0
43	11565.0
44	12799.5
45	14034.0
46	17043.5
47	20053.0
48	17793.0
49	15533.0
50	14757.0
51	13981.0
52	12926.0
53	11871.0
54	11066.5
55	10262.0
56	9737.5
57	9213.0
58	9060.0
59	8907.0
60	8820.0
61	8733.0
62	7637.0
63	6541.0
64	5445.5
65	4350.0
66	3659.0
67	2968.0
68	2512.5
69	2057.0
70	1756.0
71	1455.0
72	1219.0
73	983.0
74	821.5
75	544.5
76	429.0
77	311.0
78	193.0
79	157.5
80	122.0
81	89.0
82	56.0
83	45.5
84	35.0
85	25.5
86	16.0
87	15.5
88	15.0
89	11.5
90	8.0
91	6.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.78649471428789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.38079928373759	34.5422679681287
2	11.63824678495849	11.588550240858277
3	6.251017418199576	9.336487369503441
4	3.9262168321666935	7.81890294247261
5	2.6615660100927885	6.6255021046606455
6	1.8221959954419664	5.443245077728081
7	1.2514243854794076	4.3612823487101045
8	0.9187286342178089	3.6592222633079894
9	0.6012941559498617	2.6942695485236983
>10	1.5159531173693634	9.746275687750419
>50	0.014243854794074556	0.5085630055871015
>100	0.014243854794074556	1.4568000040522948
>500	0.001017418199576754	0.43359555058023797
>1k	0.003052254598730262	1.7850358881364004
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	1335	0.6762267056362357	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	1105	0.5597232282607044	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	1084	0.5490859542394602	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	856	0.43359555058023797	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTT	398	0.20160166954548445	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCT	312	0.1580394997441989	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	250	0.12663421453862092	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCT	234	0.11852962480814917	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	208	0.1053596664961326	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	199	0.10080083477274222	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05318637010622078	0.0
2	0.0	0.0	0.0	0.3961118230768062	0.0
3	0.0	0.0	0.0	0.49286036298431257	0.0
4	0.0	0.0	0.0	0.8773218383235656	0.0
5	0.0	0.0	0.0	2.2166052912840204	0.0
6	0.0	0.0	0.0	2.5205274061767104	0.0
7	0.0	0.0	0.0	2.9424726090193953	0.0
8	0.0	0.0	0.0	3.4799082155213026	0.0
9	0.0	0.0	0.0	3.6612484107406074	0.0
10	0.0	0.0	0.0	5.1874439643600665	0.0
11	0.0	0.0	0.0	5.793768583570984	0.0
12	0.0	0.0	0.0	7.215617544410619	0.0
13	0.0	0.0	0.0	7.437480688282283	0.0
14	0.0	0.0	0.0	7.557529923664895	0.0
15	0.0	0.0	0.0	7.799148005004584	0.0
16	0.0	0.0	0.0	8.169426448315512	0.0
17	0.0	0.0	0.0	8.541224502200903	0.0
18	0.0	0.0	0.0	8.973806979064832	0.0
19	0.0	0.0	0.0	9.203268175808812	0.0
20	0.0	0.0	0.0	9.453497383737128	0.0
21	0.0	0.0	0.0	9.751847593190119	0.0
22	0.0	0.0	0.0	10.086668456430232	0.0
23	0.0	0.0	0.0	10.405786677067557	0.0
24	0.0	0.0	0.0	10.674757748747588	0.0
25	0.0	0.0	0.0	10.876359418293072	0.0
26	0.0	0.0	0.0	11.08403953013641	0.0
27	0.0	0.0	0.0	11.3256576114761	0.0
28	0.0	0.0	0.0	11.572847598255487	0.0
29	0.0	0.0	5.065368581544836E-4	11.821050658751185	0.0
30	0.0	0.0	5.065368581544836E-4	12.13814273195589	0.0
31	0.0	0.0	0.0010130737163089672	12.412179172217467	0.0
32	0.0	0.0	0.0015196105744634508	12.686722149337196	0.0
33	0.0	0.0	0.0015196105744634508	12.938470967839974	0.0
34	0.0	0.0	0.0015196105744634508	13.213013944959705	0.0
35	0.0	0.0	0.0015196105744634508	13.48705038522128	0.0
36	0.0	0.0	0.0015196105744634508	13.755514920043156	0.0
37	0.0	0.0	0.0020261474326179344	14.042214781758595	0.0
38	0.0	0.0	0.0020261474326179344	14.324862348608796	0.0
39	0.0	0.0	0.0020261474326179344	14.585222293700202	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACAC	25	3.8790356E-5	45.0	15
CGACCCG	20	7.01908E-4	45.0	32
ATTCGGC	20	7.01908E-4	45.0	17
AAGGTAT	25	3.8790356E-5	45.0	6
CCGCTGA	20	7.01908E-4	45.0	36
TACGAAT	25	3.8790356E-5	45.0	12
TGTTATG	20	7.01908E-4	45.0	1
CGAATAT	20	7.01908E-4	45.0	14
GCTACGA	20	7.01908E-4	45.0	10
ACGGCTG	255	0.0	43.23529	8
TACGGCT	215	0.0	42.90698	7
AAACGGC	65	0.0	41.538464	6
CGGCTGT	275	0.0	40.090908	9
AACGGCT	45	1.9144863E-8	40.0	7
TGTATGG	35	6.222035E-6	38.571426	2
CTATCTC	130	0.0	38.076927	5
AGGCGAT	95	0.0	37.894737	7
CGTTTTT	475	0.0	37.894737	1
GTCATAC	30	1.1365003E-4	37.499996	39
CGGGTAT	30	1.1365003E-4	37.499996	6
>>END_MODULE