##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936557.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51482
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.018550172875955	33.0	31.0	34.0	30.0	34.0
2	32.157899848490736	33.0	31.0	34.0	30.0	34.0
3	32.06670292529428	33.0	31.0	34.0	30.0	34.0
4	35.80037683073696	37.0	35.0	37.0	35.0	37.0
5	35.841556272095104	37.0	35.0	37.0	35.0	37.0
6	35.816615516102715	37.0	35.0	37.0	35.0	37.0
7	26.599763023969544	35.0	10.0	37.0	0.0	37.0
8	31.044850627403754	35.0	19.0	37.0	17.0	37.0
9	35.86774018103415	39.0	32.0	39.0	32.0	39.0
10	36.65673439260324	38.0	35.0	39.0	33.0	39.0
11	37.02742706188571	39.0	37.0	39.0	34.0	39.0
12	37.0551260634785	39.0	37.0	39.0	34.0	39.0
13	36.965230566023074	39.0	37.0	39.0	33.0	39.0
14	38.15587972495241	40.0	37.0	41.0	33.0	41.0
15	38.281982051979334	40.0	37.0	41.0	34.0	41.0
16	38.21316188182277	40.0	37.0	41.0	34.0	41.0
17	38.137057612369375	40.0	37.0	41.0	34.0	41.0
18	38.029719125131116	40.0	37.0	41.0	33.0	41.0
19	37.912260595936445	40.0	36.0	41.0	33.0	41.0
20	37.93461792471155	40.0	36.0	41.0	33.0	41.0
21	37.89860533778796	40.0	36.0	41.0	33.0	41.0
22	37.87440270385766	40.0	36.0	41.0	33.0	41.0
23	37.8212579153879	40.0	36.0	41.0	33.0	41.0
24	37.751602501845305	40.0	36.0	41.0	33.0	41.0
25	37.642651800629345	39.0	36.0	41.0	33.0	41.0
26	37.52047317509032	39.0	35.0	41.0	33.0	41.0
27	37.404549162814185	39.0	35.0	41.0	33.0	41.0
28	37.36051435453168	39.0	35.0	41.0	33.0	41.0
29	37.331280836020355	39.0	35.0	41.0	33.0	41.0
30	37.2149489141836	39.0	35.0	41.0	32.0	41.0
31	37.118623985082166	39.0	35.0	41.0	32.0	41.0
32	36.98092537197467	39.0	35.0	41.0	31.0	41.0
33	36.865991997202904	39.0	35.0	41.0	31.0	41.0
34	36.74556155549512	39.0	35.0	41.0	31.0	41.0
35	36.70818927003613	39.0	35.0	41.0	31.0	41.0
36	36.531137096460895	39.0	35.0	41.0	30.0	41.0
37	36.3953032127734	39.0	35.0	41.0	30.0	41.0
38	36.312050813876695	39.0	35.0	40.0	30.0	41.0
39	36.278466260052056	39.0	35.0	40.0	30.0	41.0
40	36.093430713647486	39.0	35.0	40.0	30.0	41.0
41	36.02235732877511	38.0	35.0	40.0	29.0	41.0
42	35.97233984693679	38.0	35.0	40.0	29.0	41.0
43	35.92704246144284	38.0	35.0	40.0	29.0	41.0
44	35.7296919311604	38.0	35.0	40.0	28.0	41.0
45	35.62122683656423	38.0	35.0	40.0	28.0	41.0
46	35.52853424497883	38.0	35.0	40.0	27.0	41.0
47	35.417233207723086	38.0	34.0	40.0	27.0	41.0
48	35.3507827978711	38.0	34.0	40.0	27.0	41.0
49	35.19637931704285	38.0	34.0	40.0	26.0	41.0
50	34.98098364476905	38.0	34.0	40.0	26.0	41.0
51	32.837399479429706	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	5.0
11	2.0
12	2.0
13	6.0
14	4.0
15	8.0
16	6.0
17	13.0
18	30.0
19	43.0
20	57.0
21	89.0
22	146.0
23	210.0
24	306.0
25	404.0
26	519.0
27	544.0
28	687.0
29	784.0
30	1003.0
31	1271.0
32	1619.0
33	2289.0
34	3801.0
35	4931.0
36	4900.0
37	7765.0
38	9866.0
39	10162.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88807738627093	20.071092809137177	24.824210403636222	14.216619400955674
2	30.026028514820712	25.809020628569208	26.976418942543024	17.188531914067052
3	31.735363816479545	24.63579503515792	27.254185929062587	16.37465521929995
4	28.984887921992154	26.03239967367235	26.55879724952411	18.42391515481139
5	25.162192611009672	30.276601530632064	25.467153568237443	19.094052290120818
6	26.045996659026454	34.58684588788314	25.01651062507284	14.35064682801756
7	60.41140592828561	28.62165417038965	6.759644147469018	4.207295753855717
8	82.59780117322559	5.605842818849307	7.499708636028127	4.296647371896974
9	76.17031195369255	8.200924595004079	9.374150188415369	6.254613262887999
10	41.7310904782254	29.183015422866244	15.549123965657898	13.536770133250457
11	32.46183131968455	25.55456275979954	25.22435025834272	16.759255662173185
12	29.216036673011924	23.320772308768113	27.86022299055981	19.602968027660154
13	24.319179519055204	27.67569247503982	28.21180218328736	19.793325822617614
14	20.205120236199058	30.179480206674175	27.937920049726117	21.677479507400648
15	19.212540305349442	25.803193349131735	35.05691309583932	19.9273532496795
16	21.1413697991531	24.37550988695078	33.24462919078513	21.23849112311099
17	22.32430752496018	24.02004584126491	29.035391010450258	24.620255623324656
18	23.796666796161762	25.152480478613885	29.528767336156324	21.522085389068025
19	25.387514082591974	26.183908939046656	26.397575851754013	22.031001126607357
20	26.593760926148946	25.622547686570062	27.95540188803854	19.828289499242455
21	24.171555106639214	26.795773279981354	28.124392991725262	20.90827862165417
22	23.07214171943592	25.168019890447148	27.087137251855015	24.672701138261914
23	22.45250767258459	27.724253137018763	27.4833922536032	22.339846936793442
24	22.050425391398935	24.909677168719163	30.08818616215376	22.951711277728137
25	20.58777825259314	27.667922769123187	28.270074977662098	23.474224000621575
26	20.469290237364515	29.78711005788431	26.85210364787693	22.891496056874246
27	21.23849112311099	29.132512334408144	27.57857115108193	22.050425391398935
28	19.81469251388835	28.58669049376481	30.19501961850744	21.4035973738394
29	21.428848918068454	27.116273649042384	29.07423954003341	22.380637892855756
30	22.060137523794722	27.524183209665516	28.949924245367313	21.46575502117245
31	24.309467386659414	27.30080416456237	25.925566217318675	22.46416223145954
32	24.82615283011538	28.227341595120624	26.0945573210054	20.851948253758597
33	23.612136280641778	27.95540188803854	26.751097470960723	21.68136436035896
34	21.82121906685832	27.652383357289928	27.370731517812054	23.155666058039703
35	23.19451458762286	26.290742395400336	28.068062623829686	22.44668039314712
36	23.899615399557128	27.27749504681248	27.836913872809916	20.98597568082048
37	22.91286274814498	28.299211374849463	28.340002330911773	20.44792354609378
38	23.788897090245133	26.665630705877785	26.61707004389884	22.928402159978244
39	23.98508216464007	26.32959092498349	27.846626005205703	21.83870090517074
40	24.517307019929298	25.274853346800825	29.28984887921992	20.91799075404996
41	21.30647604988151	25.65168408375743	28.516763140515135	24.525076725845928
42	23.42760576512179	26.011032982401616	26.93562798648071	23.625733265995883
43	24.185152091993317	26.424769822462217	26.786061147585567	22.6040169379589
44	21.287051785089933	27.50281651839478	27.611592401227615	23.598539295287672
45	22.149489141835982	27.55137718037372	26.98807350141797	23.311060176372326
46	23.169263043393805	27.147352472708906	27.51058622431141	22.172798259585875
47	21.426906491589293	27.186201002292066	28.97129093663805	22.415601569480597
48	23.221708558331066	25.288450332154927	28.58086321432734	22.908977895186666
49	22.180567965502508	24.690182976574338	29.402509615011073	23.726739442912088
50	20.42072957538557	26.54714269064916	29.202439687657822	23.82968804630745
51	20.38770832523989	26.240239306942232	27.73008041645624	25.64197195136164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	32.0
1	65.0
2	98.0
3	171.0
4	244.0
5	183.0
6	122.0
7	116.5
8	111.0
9	119.0
10	127.0
11	122.0
12	117.0
13	125.0
14	133.0
15	126.0
16	119.0
17	122.0
18	125.0
19	121.0
20	117.0
21	152.0
22	187.0
23	235.5
24	284.0
25	316.0
26	452.0
27	556.0
28	633.5
29	711.0
30	847.5
31	984.0
32	1073.0
33	1162.0
34	1308.5
35	1455.0
36	1571.0
37	1687.0
38	1839.5
39	1992.0
40	2313.5
41	2635.0
42	3004.0
43	3373.0
44	3671.0
45	3969.0
46	4314.0
47	4659.0
48	4713.0
49	4767.0
50	4576.5
51	4386.0
52	3898.5
53	3411.0
54	3061.0
55	2711.0
56	2513.5
57	2316.0
58	2208.5
59	2101.0
60	1924.5
61	1748.0
62	1628.5
63	1509.0
64	1308.5
65	1108.0
66	908.5
67	709.0
68	571.0
69	433.0
70	378.0
71	323.0
72	283.5
73	244.0
74	188.0
75	113.5
76	95.0
77	74.0
78	53.0
79	43.5
80	34.0
81	27.5
82	21.0
83	19.0
84	17.0
85	12.0
86	7.0
87	5.0
88	3.0
89	3.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	51482.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.89825570102172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.59560714992415	41.57958121285109
2	13.211529582481365	15.562720951012004
3	6.787151243321681	11.992541082320034
4	3.8783721390409602	9.137174157958121
5	2.4635578128091815	7.254962899654248
6	1.2960886485060352	4.580241637854007
7	0.7750148407097157	3.1952915582145214
8	0.43532748499439355	2.0512023619905984
9	0.17479058109623374	0.9265374305582533
>10	0.3627729041619946	2.8340002330911775
>50	0.016489677461908846	0.6798492677052174
>100	0.0032979354923817687	0.205897206790723
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.205897206790723	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	79	0.15345169185346336	No Hit
CCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	77	0.14956683889514782	No Hit
GCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGC	71	0.13791228002020123	No Hit
CTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCGTCTTCTGCT	64	0.12431529466609689	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAACGTTGGTCGTATGCCG	59	0.11460316227030808	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16510625072840993	0.0
2	0.0	0.0	0.0	0.6390583116429043	0.0
3	0.0	0.0	0.0	0.8527252243502583	0.0
4	0.0	0.0	0.0	1.1246649314323454	0.0
5	0.0	0.0	0.0	1.8977506701371354	0.0
6	0.0	0.0	0.0	2.360048172176683	0.0
7	0.0	0.0	0.0	2.804863835903811	0.0
8	0.0	0.0	0.0	3.818810458024164	0.0
9	0.0	0.0	0.0	4.257798842313818	0.0
10	0.0	0.0	0.0	5.219299949496912	0.0
11	0.0	0.0	0.0	6.128355541742745	0.0
12	0.0	0.0	0.0	7.017986869197001	0.0
13	0.0	0.0	0.0	7.3326599588205585	0.0
14	0.0	0.0	0.0	7.451147974049182	0.0
15	0.0	0.0	0.0	7.6900664309855875	0.0
16	0.0	0.0	0.0	8.167903344858397	0.0
17	0.0	0.0	0.0	8.69624334718931	0.0
18	0.0	0.0	0.0	9.40717143856105	0.0
19	0.0	0.0	0.0	9.67716871916398	0.0
20	0.0	0.0	0.0	10.052057029641428	0.0
21	0.0	0.0	0.0	10.42500291363972	0.0
22	0.0	0.0	0.0	10.82320034186706	0.0
23	0.0	0.0	0.0	11.14370071092809	0.0
24	0.0	0.0	0.0	11.470028359426596	0.0
25	0.0	0.0	0.0	11.749737772425314	0.0
26	0.0	0.0	0.0	11.990598655840877	0.0
27	0.0	0.0	0.0	12.25671108348549	0.0
28	0.0	0.0	0.0	12.52670836408842	0.0
29	0.0	0.0	0.0	12.81224505652461	0.0
30	0.0	0.0	0.0	13.2220970436269	0.0
31	0.0	0.0	0.0	13.529000427333825	0.0
32	0.0	0.0	0.0	13.835903811040753	0.0
33	0.0	0.0	0.0	14.144749621226836	0.0
34	0.0	0.0	0.0	14.41863175478808	0.0
35	0.0	0.0	0.0	14.70611087370343	0.0
36	0.0	0.0	0.0	15.02855366924362	0.0
37	0.0	0.0	0.0	15.310205508721495	0.0
38	0.0	0.0	0.0	15.609339186511791	0.0
39	0.0	0.0	0.0	15.953148673322715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACTAT	30	2.125198E-6	45.000004	23
TAGGGAC	30	2.125198E-6	45.000004	5
CCTATGC	35	1.1819611E-7	45.0	35
TCAAGGG	20	6.9705734E-4	45.0	4
CTGGGTT	25	3.838919E-5	45.0	5
AAGGGTG	20	6.9705734E-4	45.0	6
GCTACGA	35	1.1819611E-7	45.0	10
TACGAAT	35	1.1819611E-7	45.0	12
CTACGAA	35	1.1819611E-7	45.0	11
CTATGAA	35	1.1819611E-7	45.0	26
CCCCACG	20	6.9705734E-4	45.0	38
TTGCTAG	20	6.9705734E-4	45.0	1
GCCCCCC	20	6.9705734E-4	45.0	35
AGGGATA	55	5.820766E-11	40.909092	6
TCAGGGG	40	3.3731703E-7	39.375	4
AGCTACG	40	3.3731703E-7	39.375	9
CGAATAT	40	3.3731703E-7	39.375	14
AGACCTA	40	3.3731703E-7	39.375	32
ACCTATG	40	3.3731703E-7	39.375	34
ACTATGA	40	3.3731703E-7	39.375	25
>>END_MODULE