##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936553.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	210139
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06542812138632	33.0	31.0	34.0	30.0	34.0
2	32.19134953530758	33.0	31.0	34.0	30.0	34.0
3	32.06986803972609	33.0	31.0	34.0	30.0	34.0
4	35.81783486168679	37.0	35.0	37.0	35.0	37.0
5	35.87932273399988	37.0	35.0	37.0	35.0	37.0
6	35.84525004877724	37.0	35.0	37.0	35.0	37.0
7	26.705423552981596	36.0	10.0	37.0	0.0	37.0
8	31.112011573291962	36.0	19.0	37.0	17.0	37.0
9	35.99453694935257	39.0	32.0	39.0	32.0	39.0
10	36.743246137080696	38.0	35.0	39.0	33.0	39.0
11	37.052308234073635	39.0	37.0	39.0	34.0	39.0
12	37.04905324570879	39.0	37.0	39.0	34.0	39.0
13	36.963343310856146	39.0	37.0	39.0	33.0	39.0
14	38.041772350682166	40.0	37.0	41.0	33.0	41.0
15	38.181341873712164	40.0	37.0	41.0	33.0	41.0
16	38.165685570027456	40.0	37.0	41.0	33.0	41.0
17	38.10225136695235	40.0	37.0	41.0	33.0	41.0
18	37.995564840415156	40.0	37.0	41.0	33.0	41.0
19	37.8817116289694	40.0	37.0	41.0	33.0	41.0
20	37.9177163686893	40.0	36.0	41.0	33.0	41.0
21	37.8513602900937	40.0	36.0	41.0	33.0	41.0
22	37.87557283512342	40.0	36.0	41.0	33.0	41.0
23	37.8367128424519	40.0	36.0	41.0	33.0	41.0
24	37.76467956923751	40.0	36.0	41.0	33.0	41.0
25	37.6739919767392	40.0	36.0	41.0	33.0	41.0
26	37.534246379777194	40.0	35.0	41.0	33.0	41.0
27	37.419441417347564	39.0	35.0	41.0	33.0	41.0
28	37.38094784880484	39.0	35.0	41.0	33.0	41.0
29	37.31483446671013	39.0	35.0	41.0	32.0	41.0
30	37.192215628702904	39.0	35.0	41.0	32.0	41.0
31	37.05783314853502	39.0	35.0	41.0	31.0	41.0
32	36.92529706527584	39.0	35.0	41.0	31.0	41.0
33	36.81723049981203	39.0	35.0	41.0	31.0	41.0
34	36.64789972351634	39.0	35.0	41.0	31.0	41.0
35	36.533860920628726	39.0	35.0	41.0	30.0	41.0
36	36.32570822170087	39.0	35.0	41.0	30.0	41.0
37	36.235515539714186	39.0	35.0	41.0	30.0	41.0
38	36.02937103536231	39.0	35.0	40.0	29.0	41.0
39	35.93621364906086	39.0	35.0	40.0	29.0	41.0
40	35.745401853059164	38.0	35.0	40.0	28.0	41.0
41	35.64818049005658	38.0	35.0	40.0	27.0	41.0
42	35.57767953592622	38.0	35.0	40.0	27.0	41.0
43	35.561994679712	38.0	35.0	40.0	27.0	41.0
44	35.36943166189998	38.0	34.0	40.0	26.0	41.0
45	35.25115280837922	38.0	34.0	40.0	26.0	41.0
46	35.17715416938312	38.0	34.0	40.0	26.0	41.0
47	35.01322933867583	38.0	34.0	40.0	25.0	41.0
48	34.92343639210237	38.0	34.0	40.0	25.0	41.0
49	34.79472634779836	37.0	34.0	40.0	24.0	41.0
50	34.56992752416258	37.0	34.0	40.0	24.0	41.0
51	32.51149953126264	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	18.0
10	16.0
11	16.0
12	8.0
13	8.0
14	12.0
15	16.0
16	27.0
17	69.0
18	115.0
19	185.0
20	307.0
21	454.0
22	700.0
23	1001.0
24	1336.0
25	1832.0
26	2441.0
27	2776.0
28	3101.0
29	3560.0
30	4357.0
31	5301.0
32	6860.0
33	9785.0
34	15291.0
35	17747.0
36	19767.0
37	31905.0
38	39363.0
39	41752.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23775691328121	19.105925125750097	23.61627303832225	15.04004492264644
2	30.311365334373914	24.876867216461484	27.280514326231685	17.531253122932917
3	32.0368898681349	24.618942699832015	27.28479720565911	16.05937022637397
4	28.695768039250208	25.860025982801858	27.146793312997588	18.29741266495034
5	26.00231275489081	30.367518642422397	25.25566410804277	18.37450449464402
6	27.84633028614393	34.75604242905886	23.581058251918968	13.816569032878236
7	62.062729907347034	28.205140407063894	6.158304741147526	3.5738249444415366
8	85.11937336715222	4.875344414887288	6.833572064205122	3.1717101537553716
9	78.88968730221426	6.935885294971424	8.714707883829274	5.459719518985053
10	45.006400525366544	25.505022865817388	15.544948819590843	13.943627789225227
11	33.841409733557306	25.93949718995522	23.20606836427317	17.013024712214296
12	30.683499969068095	23.25127653600712	27.127282417828198	18.93794107709659
13	26.34827423752849	24.953007295171293	29.2791913923641	19.419527074936113
14	21.899790138908056	26.435359452552838	30.67493421021324	20.98991619832587
15	20.928528259866088	24.910654376388962	33.51638677256483	20.644430591180125
16	25.581162944527193	24.505208457259243	29.60516610434046	20.3084624938731
17	26.750864903706596	23.384045798257343	27.524162578103066	22.340926719932995
18	27.292887089022027	23.889425570693685	27.994327564136118	20.823359776148166
19	26.94740148187628	25.265657493373432	26.533389803891712	21.253551220858576
20	27.421373471844827	26.60381937669828	26.953111987779515	19.02169516367738
21	26.48960925863357	25.509305745244816	28.485906947306304	19.515178048815308
22	25.991367618576277	23.287443073394275	28.578226792741944	22.1429625152875
23	24.5309057338238	25.628274618228886	28.603448193814568	21.23737145413274
24	23.69241311703206	25.113377335953825	29.17545053512199	22.01875901189213
25	25.083873055453772	26.34351548260913	26.67853182893228	21.89407963300482
26	23.670998719894925	26.517210037165878	26.984043894755377	22.82774734818382
27	23.080913109893928	26.44582871337543	28.258914337652698	22.214343839077944
28	22.29191154426356	26.50245789691585	28.44307815303204	22.76255240578855
29	24.202075768895824	26.177434935923365	27.466581643578774	22.153907651602033
30	25.305155159204144	25.020581615026245	27.76971433194219	21.904548893827418
31	25.78007890015656	25.57783181608364	27.157262573820184	21.48482670993961
32	27.151552067916953	25.73439485293068	26.40014466614955	20.71390841300282
33	26.48056762428678	26.35350886793979	25.570693683704594	21.59522982406883
34	24.256325574976564	26.600964123746664	28.605351695782318	20.537358605494457
35	24.509967212178605	27.11538553052979	27.00688591836832	21.367761338923284
36	25.779603024664627	27.96720266109575	25.3570255878252	20.896168726414423
37	24.730773440437044	28.63152484783881	25.664441155616046	20.9732605561081
38	24.40051584903326	27.763052075055082	25.585445823954622	22.250986251957038
39	24.43239950699299	26.280224042181604	26.239298749875083	23.048077700950323
40	25.17095827047811	25.064362160284382	27.569370749837013	22.19530881940049
41	22.68022594568357	25.519775006067412	27.358082031417304	24.441917016831717
42	23.385473424733153	26.3325703462946	27.276707322296197	23.00524890667606
43	23.642922065870685	25.841942714108278	27.466581643578774	23.04855357644226
44	23.989359424000302	25.765802635398476	27.100633390279764	23.144204550321454
45	23.1408734218779	25.559272671898125	26.965960626061797	24.33389328016218
46	23.465896382870387	26.09463260032645	26.900765683666528	23.53870533313664
47	22.634541898457687	25.98565711267304	28.6129657036533	22.766835285215976
48	23.818520122395174	26.446780464359303	27.65217308543393	22.082526327811593
49	23.74000066622569	25.91284816240679	27.53891471835309	22.808236453014434
50	21.945950061625876	26.619047392440244	27.763052075055082	23.671950470878798
51	22.556498317780136	25.896668395680955	26.30544544325423	25.241387843284684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	204.5
2	332.0
3	865.0
4	1398.0
5	985.0
6	572.0
7	510.0
8	448.0
9	459.0
10	470.0
11	465.5
12	461.0
13	485.5
14	510.0
15	507.5
16	505.0
17	497.5
18	490.0
19	493.0
20	496.0
21	589.0
22	682.0
23	823.5
24	965.0
25	1109.5
26	1587.0
27	1920.0
28	2294.0
29	2668.0
30	2919.5
31	3171.0
32	3763.5
33	4356.0
34	5148.0
35	5940.0
36	6440.0
37	6940.0
38	7558.0
39	8176.0
40	8934.5
41	9693.0
42	10448.5
43	11204.0
44	12092.0
45	12980.0
46	14343.0
47	15706.0
48	15602.0
49	15498.0
50	15516.0
51	15534.0
52	15099.5
53	14665.0
54	13791.0
55	12917.0
56	12434.0
57	11951.0
58	11493.5
59	11036.0
60	10510.0
61	9984.0
62	8924.0
63	7864.0
64	6843.5
65	5823.0
66	5151.0
67	4479.0
68	3825.0
69	3171.0
70	2603.5
71	2036.0
72	1775.5
73	1515.0
74	1247.0
75	782.0
76	585.0
77	441.5
78	298.0
79	231.5
80	165.0
81	134.5
82	104.0
83	75.5
84	47.0
85	42.5
86	38.0
87	27.0
88	16.0
89	16.0
90	16.0
91	9.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	210139.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.14926786555566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.41467919538954	31.314034995883677
2	11.41816126525298	10.767158880550493
3	6.628044287891481	9.375223066636845
4	4.346026907821032	8.196479473110656
5	3.146983720061769	7.418898919286758
6	2.2567849897556496	6.384345599817263
7	1.6592819871012021	5.476375161202823
8	1.195006005308895	4.507492659620537
9	0.9204775986838785	3.905986037813067
>10	1.9963867217069207	11.401025035809631
>50	0.012111547351103666	0.400687164210356
>100	0.0050464780629598605	0.5272700450654091
>500	0.001009295612591972	0.3250229609924859
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.3250229609924859	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCGTCTTC	300	0.14276264758088691	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGCCG	284	0.13514863970990632	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGATCATCGTATGC	268	0.12753463183892566	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041877043290393504	0.0
2	0.0	0.0	0.0	0.2241373567019925	0.0
3	0.0	0.0	0.0	0.29884980893599	0.0
4	0.0	0.0	0.0	0.39402490732324796	0.0
5	0.0	0.0	0.0	0.8275474804772079	0.0
6	0.0	0.0	0.0	1.1125969001470455	0.0
7	0.0	0.0	0.0	1.3586245294781074	0.0
8	0.0	0.0	0.0	1.8221272586240536	0.0
9	0.0	0.0	0.0	2.081955277221268	0.0
10	0.0	0.0	0.0	2.7267665687949405	0.0
11	0.0	0.0	0.0	3.285920271820081	0.0
12	0.0	0.0	0.0	3.8798128857565706	0.0
13	0.0	0.0	0.0	4.091101604176283	0.0
14	0.0	0.0	0.0	4.195318336910331	0.0
15	0.0	0.0	0.0	4.370440517942885	0.0
16	0.0	0.0	0.0	4.638834295394953	0.0
17	0.0	0.0	0.0	4.957670874992267	0.0
18	0.0	0.0	0.0	5.276983330081517	0.0
19	0.0	0.0	0.0	5.473519908251205	0.0
20	0.0	0.0	0.0	5.710505903235478	0.0
21	0.0	0.0	0.0	5.991748318969825	0.0
22	0.0	0.0	0.0	6.273942485688044	0.0
23	0.0	0.0	0.0	6.551853772978838	0.0
24	0.0	0.0	0.0	6.7774187561566395	0.0
25	0.0	0.0	0.0	6.984900470640862	0.0
26	0.0	0.0	0.0	7.183340550778294	0.0
27	0.0	0.0	0.0	7.456968958641661	0.0
28	0.0	0.0	0.0	7.663974797633947	0.0
29	0.0	0.0	0.0	7.90476779655371	0.0
30	0.0	0.0	0.0	8.1983829750784	0.0
31	0.0	0.0	0.0	8.405388814070687	0.0
32	0.0	0.0	0.0	8.642374809054958	0.0
33	0.0	0.0	0.0	8.868891543216632	0.0
34	0.0	0.0	0.0	9.104449911725096	0.0
35	0.0	0.0	0.0	9.399968592217531	0.0
36	0.0	0.0	0.0	9.669789996145408	0.0
37	0.0	0.0	0.0	9.93009389023456	0.0
38	0.0	0.0	0.0	10.232274827614104	0.0
39	0.0	0.0	0.0	10.71243319897782	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	20	7.020117E-4	45.0	2
GAATCTA	20	7.020117E-4	45.0	1
TATGCGG	25	3.8798928E-5	45.0	2
CCGCTAT	20	7.020117E-4	45.0	12
CGCTATA	20	7.020117E-4	45.0	13
TAGGGTC	20	7.020117E-4	45.0	5
CGAATGA	20	7.020117E-4	45.0	45
CGTAAGG	45	3.8198777E-10	45.0	3
ACGTAAG	20	7.020117E-4	45.0	2
GGCGATT	90	0.0	40.0	8
CATGCGG	40	3.4402728E-7	39.375	2
GACACGA	40	3.4402728E-7	39.375	25
CGACCAA	40	3.4402728E-7	39.375	29
AGACACG	40	3.4402728E-7	39.375	24
ACGACCA	40	3.4402728E-7	39.375	28
ACGTGAG	35	6.2239487E-6	38.571426	44
TATACTG	30	1.1367491E-4	37.500004	18
GCGATTG	55	2.7266651E-9	36.81818	9
GCGATGT	55	2.7266651E-9	36.81818	9
ACACGAC	50	4.8465154E-8	36.0	26
>>END_MODULE