FastQCFastQC Report
Sat 14 Jan 2017
SRR2936552.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936552.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410179
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47611.1607127619892779No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC46191.1260937298106435No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG23990.5848666070179117No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC16400.3998254420630992No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT11520.28085299344920145No Hit
GAATCTATCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC9020.21990399313470463No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.21624705311583478No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTT6640.16188054483530362No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC6410.1562732368063699TruSeq Adapter, Index 13 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG5550.13530678069818297No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT5530.134819188695667No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT5150.1255549406478635No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC4640.11312134458370614No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.1080016285572884No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT207.0279744E-445.0000044
CGCAATG207.0279744E-445.00000430
CATGCGG502.1827873E-1145.02
TAGACGG302.1620763E-644.9999962
CGTTTTT16800.043.5267871
AGACACG750.042.024
AACACGT750.042.041
CGTTTTA3800.041.447371
TACGGCT1800.040.07
GAATCTG7850.039.8407631
ACACGAC800.039.37500426
GACACGA800.039.37500425
TGATACC3850.038.571434
TAGCGAC356.238466E-638.57142629
TTGAGCG356.238466E-638.5714261
TTCGGTA356.238466E-638.57142624
TCGGTAG356.238466E-638.57142625
AATCTGT8150.038.098162
ACACGCG1950.038.07692336
ACAACGA900.037.513
Produced by FastQC (version 0.11.5)