##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936552.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410179
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.836861467798204	31.0	31.0	34.0	30.0	34.0
2	31.931717615967663	33.0	31.0	34.0	30.0	34.0
3	31.54909685771334	33.0	31.0	34.0	28.0	34.0
4	35.468522279297574	37.0	35.0	37.0	33.0	37.0
5	35.65005278183427	37.0	35.0	37.0	33.0	37.0
6	35.628671872523945	37.0	35.0	37.0	33.0	37.0
7	26.809278388215876	35.0	11.0	37.0	0.0	37.0
8	31.03880013360021	35.0	19.0	37.0	17.0	37.0
9	35.82608324658259	39.0	32.0	39.0	32.0	39.0
10	36.63882597597634	38.0	35.0	39.0	33.0	39.0
11	36.91838685061887	39.0	37.0	39.0	33.0	39.0
12	36.89481177729723	39.0	35.0	39.0	33.0	39.0
13	36.8194178639082	39.0	35.0	39.0	33.0	39.0
14	37.906145853395714	40.0	37.0	41.0	33.0	41.0
15	37.97303860022088	40.0	37.0	41.0	33.0	41.0
16	37.952535356515085	40.0	37.0	41.0	33.0	41.0
17	37.840196597095414	40.0	36.0	41.0	33.0	41.0
18	37.63143164325819	39.0	36.0	41.0	33.0	41.0
19	37.47733306678304	39.0	36.0	41.0	32.0	41.0
20	37.45181006341134	39.0	35.0	41.0	32.0	41.0
21	37.43252336175182	39.0	35.0	41.0	33.0	41.0
22	37.47319584864169	39.0	35.0	41.0	33.0	41.0
23	37.4522098888534	39.0	35.0	41.0	33.0	41.0
24	37.35374799782534	39.0	35.0	41.0	33.0	41.0
25	37.26005231862187	39.0	35.0	41.0	33.0	41.0
26	37.11074677153145	39.0	35.0	41.0	32.0	41.0
27	37.02165639879175	39.0	35.0	41.0	32.0	41.0
28	36.98191521262668	39.0	35.0	41.0	32.0	41.0
29	36.92944299927593	39.0	35.0	41.0	32.0	41.0
30	36.7783333617762	39.0	35.0	41.0	31.0	41.0
31	36.565143510516144	39.0	35.0	40.0	31.0	41.0
32	36.3679076695784	39.0	35.0	40.0	30.0	41.0
33	36.19932761062853	39.0	35.0	40.0	30.0	41.0
34	35.90509021671026	38.0	35.0	40.0	29.0	41.0
35	35.632277615382556	38.0	35.0	40.0	27.0	41.0
36	35.473485965883185	38.0	34.0	40.0	26.0	41.0
37	35.33656037973665	38.0	34.0	40.0	25.0	41.0
38	35.0853481041204	38.0	34.0	40.0	24.0	41.0
39	34.93045962860117	38.0	34.0	40.0	24.0	41.0
40	34.66558258711441	38.0	34.0	40.0	23.0	41.0
41	34.50088376050456	38.0	33.0	40.0	23.0	41.0
42	34.41161054076391	38.0	33.0	40.0	22.0	41.0
43	34.34305266725015	38.0	33.0	40.0	22.0	41.0
44	34.14924947401013	37.0	33.0	40.0	21.0	41.0
45	34.06120498611582	37.0	33.0	40.0	21.0	41.0
46	33.84480190355918	37.0	33.0	40.0	20.0	41.0
47	33.64219767467374	37.0	33.0	40.0	20.0	41.0
48	33.46201048810397	36.0	32.0	40.0	18.0	41.0
49	33.30166829603661	36.0	32.0	40.0	18.0	41.0
50	33.105836720066115	36.0	32.0	40.0	17.0	41.0
51	30.81577311368939	35.0	27.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	16.0
9	26.0
10	23.0
11	20.0
12	15.0
13	15.0
14	23.0
15	29.0
16	75.0
17	128.0
18	252.0
19	408.0
20	730.0
21	1104.0
22	1661.0
23	2559.0
24	3866.0
25	5507.0
26	7198.0
27	8372.0
28	8913.0
29	9278.0
30	10695.0
31	13115.0
32	15897.0
33	21660.0
34	31118.0
35	35528.0
36	38020.0
37	57065.0
38	67833.0
39	69024.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.190116997701004	17.095706996213845	20.8516281915944	19.862547814490746
2	32.22666201828957	26.481853044646364	24.72969118360521	16.561793753458858
3	29.44324307192713	26.43699458041489	28.83814139680481	15.281620950853164
4	26.275114035579588	23.33615324041455	32.94122809797674	17.447504626029122
5	25.583952372013197	27.11596644391839	28.328363958174357	18.97171722589406
6	25.6054064201239	32.3373454028607	28.504628467083883	13.552619709931518
7	57.121403094746434	27.16887017619137	11.66880800821105	4.040918720851141
8	76.37421711009095	5.79259298988978	13.324426652754044	4.508763247265218
9	71.3115493479676	5.724573905538801	14.388839994246414	8.57503675224719
10	42.79765663283591	20.85699170362208	21.745628128207443	14.599723535334574
11	31.76588757591198	22.958513234465926	27.050385319579988	18.225213870042104
12	28.12260013311262	20.80676972736293	31.65569178334337	19.414938356181082
13	24.101428888363372	21.775127444359658	35.18171334953764	18.941730317739328
14	20.06928682355752	24.41080601395976	34.36645952133093	21.15344764115179
15	19.37666238398358	23.167202611542763	38.00023892008123	19.455896084392425
16	24.352051177656584	24.284032093305605	31.132261768642472	20.23165496039534
17	24.581219418839094	23.642117221993324	30.488640325321388	21.288023033846198
18	24.340836561598717	24.059495976146998	31.389466549969647	20.210200912284638
19	24.271354701240192	25.52787929172386	28.242791561732805	21.95797444530315
20	24.239173629074138	27.31295361293484	30.085645535241927	18.36222722274909
21	24.2945153213597	25.565180079916328	31.420428642129412	18.71987595659456
22	23.654550818057483	22.51748626819023	31.52014120664393	22.30782170710836
23	22.728369809278387	24.4181198939975	32.62258672433255	20.230923572391564
24	24.373749021768546	22.959000826468447	30.783877282844806	21.883372868918205
25	23.27081591207741	26.514521708814932	28.47586053893544	21.73880184017222
26	21.817791744579807	23.766696978636155	29.382781663615155	25.032729613168886
27	23.190850823664793	23.64138583398955	30.10636819534886	23.061395146996798
28	20.68462793073268	24.254045185150876	30.461822765183005	24.599504118933442
29	22.46287596390844	24.160671316669063	28.97539854551306	24.40105417390944
30	23.44415486897184	23.02872648282823	31.772713863947207	21.754404784252728
31	25.629054632245925	22.74616691737022	29.17116673452322	22.453611715860635
32	25.64612035233398	24.78015695586561	28.242303969730287	21.33141872207012
33	24.062421528162094	22.855875117936318	28.264245609843506	24.817457744058082
34	22.46750808793234	23.288369224167983	30.288971400291093	23.95515128760858
35	20.987178768293845	24.883526460398997	31.925573956735963	22.2037208145712
36	23.866897135153188	24.643874991162395	29.808693277812857	21.68053459587156
37	21.9199422691069	26.139807254881404	30.70586256244225	21.234387913569442
38	23.383937256661117	24.728472203598916	28.268146345863638	23.619444193876333
39	23.580924425677573	24.12995302051056	28.74525512032552	23.54386743348636
40	23.29129477618308	23.38930076868879	31.058147784260044	22.261256670868086
41	20.8516281915944	25.093922409484637	29.693377769217825	24.36107162970313
42	21.739289432174733	23.478286309147958	32.20788972619271	22.5745345324846
43	23.23985381991765	23.431477476906423	29.49468402819257	23.83398467498336
44	22.708134741173975	23.646749346017227	29.131915578320683	24.513200334488115
45	21.745384332206182	22.99825198267098	28.49633940304111	26.760024282081723
46	23.922970215442525	24.912781980549955	27.72594403906587	23.438303764941647
47	20.420109269367764	23.711111490349335	32.459243403489694	23.409535836793204
48	21.592768035418683	23.948324999573355	29.713125245319727	24.745781719688235
49	21.92140504511445	22.64840472086577	31.838782580288118	23.591407653731665
50	21.063486916687594	23.628708441924136	31.234656089170826	24.073148552217447
51	21.055441648646077	23.535090777441066	29.04268624186026	26.36678133205259
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	342.5
2	600.0
3	4915.0
4	9230.0
5	6200.5
6	3171.0
7	2755.5
8	2340.0
9	2201.5
10	2063.0
11	1977.0
12	1891.0
13	1871.0
14	1851.0
15	1712.0
16	1573.0
17	1571.0
18	1569.0
19	1544.0
20	1519.0
21	1486.0
22	1453.0
23	1623.0
24	1793.0
25	2204.5
26	3105.0
27	3594.0
28	3979.0
29	4364.0
30	5010.5
31	5657.0
32	6748.5
33	7840.0
34	8731.5
35	9623.0
36	10324.5
37	11026.0
38	12261.0
39	13496.0
40	14385.0
41	15274.0
42	16903.0
43	18532.0
44	21209.5
45	23887.0
46	32130.5
47	40374.0
48	35997.0
49	31620.0
50	31205.5
51	30791.0
52	28540.0
53	26289.0
54	25047.0
55	23805.0
56	22711.0
57	21617.0
58	21075.0
59	20533.0
60	19128.0
61	17723.0
62	16262.5
63	14802.0
64	13410.5
65	12019.0
66	10247.0
67	8475.0
68	7212.5
69	5950.0
70	5005.0
71	4060.0
72	3446.5
73	2833.0
74	2357.0
75	1508.0
76	1135.0
77	851.0
78	567.0
79	419.0
80	271.0
81	226.0
82	181.0
83	132.5
84	84.0
85	71.0
86	58.0
87	39.0
88	20.0
89	22.5
90	25.0
91	16.0
92	7.0
93	7.5
94	8.0
95	5.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410179.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.71269938738631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.3321372659636	33.08023423255924
2	11.23863243103062	10.724509814141891
3	6.059346994276762	8.673234048653697
4	3.828323596645486	7.306386116975346
5	2.5959442352211424	6.19297534607624
6	1.972147196436084	5.6457879798738935
7	1.4854489438200078	4.961234523825617
8	1.026224256856567	3.9171143577153034
9	0.7778436639260714	3.340171881655901
>10	1.652544706735112	9.896559970981928
>50	0.013677115248281089	0.43867345937572794
>100	0.011650875952211828	1.1613366238548137
>500	0.0035459187680644696	1.1400676076426963
>1k	0.0025327991200460496	3.5217140366677397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4761	1.1607127619892779	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	4619	1.1260937298106435	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	2399	0.5848666070179117	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1640	0.3998254420630992	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCT	1152	0.28085299344920145	No Hit
GAATCTATCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	902	0.21990399313470463	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	887	0.21624705311583478	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTT	664	0.16188054483530362	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	641	0.1562732368063699	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGAAACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	555	0.13530678069818297	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCT	553	0.134819188695667	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTT	515	0.1255549406478635	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTC	464	0.11312134458370614	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	443	0.1080016285572884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.8759200251597475E-4	0.0	0.0	0.07557676038997609	0.0
2	4.8759200251597475E-4	0.0	0.0	0.48295987849207295	0.0
3	4.8759200251597475E-4	0.0	0.0	0.6687324314506593	0.0
4	4.8759200251597475E-4	0.0	0.0	1.1919186501503003	0.0
5	4.8759200251597475E-4	0.0	0.0	3.1042544840179533	0.0
6	4.8759200251597475E-4	0.0	0.0	3.8544147798887805	0.0
7	4.8759200251597475E-4	0.0	0.0	4.538262563417435	0.0
8	4.8759200251597475E-4	0.0	0.0	5.550991152643114	0.0
9	4.8759200251597475E-4	0.0	0.0	6.120986203584289	0.0
10	4.8759200251597475E-4	0.0	0.0	7.664214891547349	0.0
11	4.8759200251597475E-4	0.0	0.0	8.301985230838243	0.0
12	4.8759200251597475E-4	0.0	0.0	9.31495761606518	0.0
13	4.8759200251597475E-4	0.0	0.0	9.588009137474128	0.0
14	4.8759200251597475E-4	0.0	0.0	9.789140838511967	0.0
15	4.8759200251597475E-4	0.0	0.0	9.981252087503261	0.0
16	4.8759200251597475E-4	0.0	0.0	10.207250980669414	0.0
17	4.8759200251597475E-4	0.0	0.0	10.44275791788463	0.0
18	4.8759200251597475E-4	0.0	0.0	10.698987515206776	0.0
19	4.8759200251597475E-4	0.0	0.0	10.880371740142719	0.0
20	4.8759200251597475E-4	0.0	0.0	11.079065481167978	0.0
21	4.8759200251597475E-4	0.0	0.0	11.302626414321553	0.0
22	4.8759200251597475E-4	0.0	0.0	11.556418051631118	0.0
23	4.8759200251597475E-4	0.0	0.0	11.802895808902942	0.0
24	4.8759200251597475E-4	0.0	0.0	12.019142862018777	0.0
25	4.8759200251597475E-4	0.0	0.0	12.220274563056616	0.0
26	4.8759200251597475E-4	0.0	0.0	12.406047116015204	0.0
27	4.8759200251597475E-4	0.0	0.0	12.644723401246774	0.0
28	4.8759200251597475E-4	0.0	0.0	12.845855102284613	0.0
29	4.8759200251597475E-4	0.0	0.0	13.07989926349228	0.0
30	4.8759200251597475E-4	0.0	0.0	13.381718713049668	0.0
31	4.8759200251597475E-4	0.0	0.0	13.628440266322752	0.0
32	4.8759200251597475E-4	0.0	0.0	13.897103459709054	0.0
33	7.313880037739621E-4	0.0	0.0	14.130903824915464	0.0
34	7.313880037739621E-4	0.0	0.0	14.38688962623635	0.0
35	7.313880037739621E-4	0.0	0.0	14.647995143583655	0.0
36	7.313880037739621E-4	0.0	0.0	14.88837800082403	0.0
37	7.313880037739621E-4	0.0	0.0	15.16703683026191	0.0
38	7.313880037739621E-4	0.0	0.0	15.50737604801806	0.0
39	7.313880037739621E-4	0.0	0.0	16.12174197118819	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	20	7.0279744E-4	45.000004	4
CGCAATG	20	7.0279744E-4	45.000004	30
CATGCGG	50	2.1827873E-11	45.0	2
TAGACGG	30	2.1620763E-6	44.999996	2
CGTTTTT	1680	0.0	43.526787	1
AGACACG	75	0.0	42.0	24
AACACGT	75	0.0	42.0	41
CGTTTTA	380	0.0	41.44737	1
TACGGCT	180	0.0	40.0	7
GAATCTG	785	0.0	39.840763	1
ACACGAC	80	0.0	39.375004	26
GACACGA	80	0.0	39.375004	25
TGATACC	385	0.0	38.57143	4
TAGCGAC	35	6.238466E-6	38.571426	29
TTGAGCG	35	6.238466E-6	38.571426	1
TTCGGTA	35	6.238466E-6	38.571426	24
TCGGTAG	35	6.238466E-6	38.571426	25
AATCTGT	815	0.0	38.09816	2
ACACGCG	195	0.0	38.076923	36
ACAACGA	90	0.0	37.5	13
>>END_MODULE